C Watts

Summary

Affiliation: University of Dundee
Country: UK

Publications

  1. ncbi request reprint Pathways of antigen processing and presentation
    C Watts
    Department of Biochemistry, University of Dundee, UK
    Rev Immunogenet 1:60-74. 1999
  2. pmc Cystatin F is a cathepsin C-directed protease inhibitor regulated by proteolysis
    Garth Hamilton
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 27:499-508. 2008
  3. pmc TLR ligand-induced podosome disassembly in dendritic cells is ADAM17 dependent
    Michele A West
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 182:993-1005. 2008
  4. pmc Internalization of exogenous cystatin F supresses cysteine proteases and induces the accumulation of single-chain cathepsin L by multiple mechanisms
    Jeff D Colbert
    Division of Cell Signaling and Immunology, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, United Kingdom
    J Biol Chem 286:42082-90. 2011
  5. pmc A multifunctional protease inhibitor to regulate endolysosomal function
    Sander I van Kasteren
    Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, U K
    ACS Chem Biol 6:1198-204. 2011
  6. pmc The endosome-lysosome pathway and information generation in the immune system
    Colin Watts
    Division of Cell Signaling and Immunology, College of Life Sciences, University of Dundee, Dundee, UK
    Biochim Biophys Acta 1824:14-21. 2012
  7. doi request reprint TLR signalling regulated antigen presentation in dendritic cells
    Colin Watts
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Curr Opin Immunol 22:124-30. 2010
  8. ncbi request reprint Immunology. The bell tolls for phagosome maturation
    Colin Watts
    Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Science 304:976-7. 2004
  9. ncbi request reprint The exogenous pathway for antigen presentation on major histocompatibility complex class II and CD1 molecules
    Colin Watts
    Division of Cell Biology and Immunology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nat Immunol 5:685-92. 2004
  10. ncbi request reprint Asparaginyl endopeptidase: case history of a class II MHC compartment protease
    Colin Watts
    Division of Cell Biology and Immunology, School of Life Science, University of Dundee, Dundee, UK
    Immunol Rev 207:218-28. 2005

Collaborators

Detail Information

Publications48

  1. ncbi request reprint Pathways of antigen processing and presentation
    C Watts
    Department of Biochemistry, University of Dundee, UK
    Rev Immunogenet 1:60-74. 1999
    ..Here, we provide an overview of what is currently known about the cellular biochemistry of antigen processing and the assembly of class I and class II MHC molecules...
  2. pmc Cystatin F is a cathepsin C-directed protease inhibitor regulated by proteolysis
    Garth Hamilton
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 27:499-508. 2008
    ..Thus, cystatin F is a latent protease inhibitor itself regulated by proteolysis in the endocytic pathway. By targeting cathepsin C, it may regulate diverse immune cell effector functions...
  3. pmc TLR ligand-induced podosome disassembly in dendritic cells is ADAM17 dependent
    Michele A West
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 182:993-1005. 2008
    ....
  4. pmc Internalization of exogenous cystatin F supresses cysteine proteases and induces the accumulation of single-chain cathepsin L by multiple mechanisms
    Jeff D Colbert
    Division of Cell Signaling and Immunology, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, United Kingdom
    J Biol Chem 286:42082-90. 2011
    ..However, we found equivalent loss of CatL activity in wild type and cystatin F-null macrophages suggesting that an inhibitory activity other than cystatin F quenches CatL activity in activated macrophages...
  5. pmc A multifunctional protease inhibitor to regulate endolysosomal function
    Sander I van Kasteren
    Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, U K
    ACS Chem Biol 6:1198-204. 2011
    ..We demonstrate that this cystatin-pepstatin inhibitor (CPI) can be taken up by cells to modulate protease activity and affect biological responses...
  6. pmc The endosome-lysosome pathway and information generation in the immune system
    Colin Watts
    Division of Cell Signaling and Immunology, College of Life Sciences, University of Dundee, Dundee, UK
    Biochim Biophys Acta 1824:14-21. 2012
    ..This article is part of a Special Issue entitled: Proteolysis 50 years after the discovery of lysosome...
  7. doi request reprint TLR signalling regulated antigen presentation in dendritic cells
    Colin Watts
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Curr Opin Immunol 22:124-30. 2010
    ..These rapid changes require activation of the MAP kinases, PI3-kinase and downstream signalling pathways and are observed in both myeloid DC and, with variations on the theme, in plasmacytoid DC...
  8. ncbi request reprint Immunology. The bell tolls for phagosome maturation
    Colin Watts
    Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Science 304:976-7. 2004
  9. ncbi request reprint The exogenous pathway for antigen presentation on major histocompatibility complex class II and CD1 molecules
    Colin Watts
    Division of Cell Biology and Immunology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nat Immunol 5:685-92. 2004
    ..Some features of CD1 antigen capture within the endocytic pathway are also discussed...
  10. ncbi request reprint Asparaginyl endopeptidase: case history of a class II MHC compartment protease
    Colin Watts
    Division of Cell Biology and Immunology, School of Life Science, University of Dundee, Dundee, UK
    Immunol Rev 207:218-28. 2005
    ..Overall, while there is undoubtedly redundancy in class II MHC antigen processing, the contributions of individual enzymes can be clearly dissected...
  11. ncbi request reprint Phagosome neutrality in host defense
    Colin Watts
    Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH
    Cell 126:17-9. 2006
    ..Here, the neutralization of phagosomal pH reduces protease activity, which preserves antigens for crosspresentation on class I MHC molecules...
  12. ncbi request reprint Proximal effects of Toll-like receptor activation in dendritic cells
    Colin Watts
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Curr Opin Immunol 19:73-8. 2007
    ..These changes, which mostly occur within minutes to a few hours after TLR engagement, are adaptations relevant to the antigen capture, processing and migratory phases of the DC life history...
  13. doi request reprint Location, location, location: identifying the neighborhoods of LPS signaling
    Colin Watts
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nat Immunol 9:343-5. 2008
  14. ncbi request reprint Creation versus destruction of T cell epitopes in the class II MHC pathway
    C Watts
    Division of Cell Biology Immunology, School of Life Sciences, University of Dundee, DD1 5EH, United Kingdom
    Ann N Y Acad Sci 987:9-14. 2003
    ..The emerging consensus seems to be that individual proteolytic enzymes make clear and non-redundant contributions to antigen processing...
  15. ncbi request reprint Antigen traffic pathways in dendritic cells
    C Watts
    Department of Biochemistry, University of Dundee, Dundee, DD1 5EH, UK
    Traffic 1:312-7. 2000
    ..Progress in DC culture methods has recently allowed in-depth studies on the cell biological features that enable them to fulfill their crucial role in the immune response...
  16. ncbi request reprint Capture and processing of exogenous antigens for presentation on MHC molecules
    C Watts
    Department of Biochemistry, Medical Sciences Institute, University of Dundee, United Kingdom
    Annu Rev Immunol 15:821-50. 1997
    ....
  17. ncbi request reprint Phagocytosis: how the phagosome became the phag-ER-some
    Colin Watts
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, MSI WTB complex, Dow Street, DD1 5EH, Dundee, UK
    Curr Biol 12:R666-8. 2002
    ..Surprisingly, the involvement of the endoplasmic reticulum in macrophage-mediated phagocytosis has only just been discovered...
  18. ncbi request reprint Modulation by epitope-specific antibodies of class II MHC-restricted presentation of the tetanus toxin antigen
    C Watts
    Department of Biochemistry, University of Dundee, UK
    Immunol Rev 164:11-6. 1998
    ..These studies show that protein/protein complexes can be processed in an unpredictable fashion by antigen presenting cells and indicate a possible mechanism whereby cryptic T-cell epitopes might be revealed in autoimmune disease...
  19. ncbi request reprint Antigen processing in the endocytic compartment
    C Watts
    Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Curr Opin Immunol 13:26-31. 2001
    ..However, the details of invariant-chain processing and antigen processing may differ depending on the allele of class II and the antigen substrate under consideration...
  20. ncbi request reprint Rac is required for constitutive macropinocytosis by dendritic cells but does not control its downregulation
    M A West
    Department of Biochemistry, MSI WTB complex, University of Dundee, Dundee, DDI 5EH, UK
    Curr Biol 10:839-48. 2000
    ..We have tested the possibility that one or more members of the Rho family of GTPases are involved in and control pinocytosis in dendritic cells...
  21. ncbi request reprint Bm-CPI-2, a cystatin homolog secreted by the filarial parasite Brugia malayi, inhibits class II MHC-restricted antigen processing
    B Manoury
    Department of Biochemistry, Wellcome Trust Biocentre, University of Dundee, DD1 5EH, Dundee, United Kingdom
    Curr Biol 11:447-51. 2001
    ....
  22. doi request reprint Distinct protease requirements for antigen presentation in vitro and in vivo
    Stephen P Matthews
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Immunol 184:2423-31. 2010
    ....
  23. ncbi request reprint An asparaginyl endopeptidase processes a microbial antigen for class II MHC presentation
    B Manoury
    Department of Biochemistry, University of Dundee, UK
    Nature 396:695-9. 1998
    ..This indicates that N-glycosylation could eliminate sites of processing by AEP in mammalian proteins, allowing preferential processing of microbial antigens...
  24. ncbi request reprint Multistep autoactivation of asparaginyl endopeptidase in vitro and in vivo
    Dongtao Ni Li
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, United Kingdom
    J Biol Chem 278:38980-90. 2003
    ..Moreover, we show that human monocyte-derived dendritic cells harbor inactive proforms of AEP that become activated upon maturation of dendritic cells with lipopolysaccharide...
  25. ncbi request reprint Membrane ruffling, macropinocytosis and antigen presentation in the absence of gelsolin in murine dendritic cells
    M A West
    Department of Biochemistry, University of Dundee, Dundee, GB
    Eur J Immunol 29:3450-5. 1999
    ..Thus the major rearrangements of the actin cytoskeleton needed for DC antigen uptake and presentation can proceed in the absence of a major actin filament regulatory protein...
  26. ncbi request reprint Asparagine deamidation perturbs antigen presentation on class II major histocompatibility complex molecules
    Catherine X Moss
    Division of Cell Biology and Immunology, University of Dundee, Scotland, United Kingdom
    J Biol Chem 280:18498-503. 2005
    ..Thus, deamidation of long-lived self-proteins may qualitatively or quantitatively affect the spectrum of self-peptides displayed to T cells and may thereby contribute to the onset or exacerbation of autoimmune disease...
  27. ncbi request reprint Structural basis of reduction-dependent activation of human cystatin F
    Alexander W Schuttelkopf
    Division of Biological Chemistry and Molecular Microbiology, Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland
    J Biol Chem 281:16570-5. 2006
    ....
  28. ncbi request reprint Characterization of transport of newly assembled, T cell-stimulatory MHC class II-peptide complexes from MHC class II compartments to the cell surface
    L Pond
    Department of Biochemistry, Medical Sciences Institute, University of Dundee, Scotland
    J Immunol 159:543-53. 1997
    ..Moreover, the brefeldin A sensitivity of this final step in the class II molecule biosynthetic pathway suggests a vesicular intermediate for transport between the MIIC and the plasma membrane...
  29. ncbi request reprint Enhanced dendritic cell antigen capture via toll-like receptor-induced actin remodeling
    Michele A West
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Science 305:1153-7. 2004
    ..Thus, the DC actin cytoskeleton can be rapidly mobilized in response to innate immune stimuli to enhance antigen capture and presentation...
  30. pmc Glycosylation directs targeting and activation of cystatin f from intracellular and extracellular sources
    Jeff D Colbert
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee, UK
    Traffic 10:425-37. 2009
    ....
  31. ncbi request reprint Asparagine endopeptidase can initiate the removal of the MHC class II invariant chain chaperone
    Benedicte Manoury
    Division of Cell Biology and Immunology, School of Life Sciences, University of Dundee, Dow Street, United Kingdom
    Immunity 18:489-98. 2003
    ..However, the initiation of Ii removal can also be performed by other proteases, reflecting the importance of this step...
  32. ncbi request reprint Antibody modulation of antigen presentation: positive and negative effects on presentation of the tetanus toxin antigen via the murine B cell isoform of FcgammaRII
    Antony N Antoniou
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, GB
    Eur J Immunol 32:530-40. 2002
    ..These results show that the B cell isoform of FcgammaRII (FcgammaRIIB1) can mediate capture and presentation of some antigen/antibody complexes and might play a role in BCR-independent antigen presentation in vivo...
  33. ncbi request reprint Destructive potential of the aspartyl protease cathepsin D in MHC class II-restricted antigen processing
    Catherine X Moss
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, University of Dundee, Dundee, UK
    Eur J Immunol 35:3442-51. 2005
    ..Our results indicate redundancy among lysosomal aspartyl proteases and show that while processing activities can be productive for MHC class II T cell epitope generation at one level, they can become destructive above an optimal level...
  34. ncbi request reprint 3-phosphoinositide-dependent kinase 1 deficiency perturbs Toll-like receptor signaling events and actin cytoskeleton dynamics in dendritic cells
    Rossana Zaru
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 283:929-39. 2008
    ..Reintroduction of PDK1 restored S6 kinase activity, increased levels of F-actin, and boosted macropinocytosis thus linking PDK1 and its downstream effectors to the unusual phenotype of PDK1(fl/-) DC...
  35. ncbi request reprint The MAPK-activated kinase Rsk controls an acute Toll-like receptor signaling response in dendritic cells and is activated through two distinct pathways
    Rossana Zaru
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nat Immunol 8:1227-35. 2007
    ..Thus, in DCs, p38 contributes to the activation of all known MK families...
  36. ncbi request reprint Destructive processing by asparagine endopeptidase limits presentation of a dominant T cell epitope in MBP
    Benedicte Manoury
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nat Immunol 3:169-74. 2002
    ..Consistent with this hypothesis, AEP is expressed abundantly in thymic antigen-presenting cells...
  37. ncbi request reprint A role for ARF6 in dendritic cell podosome formation and migration
    Henrik G Svensson
    Division of Cell Biology and Immunology, School of Life Sciences, University of Dundee, Dundee, UK
    Eur J Immunol 38:818-28. 2008
    ..They reveal for the first time a role for ARF6 in podosome formation and demonstrate functional effects of the T44N ARF6 mutant...
  38. pmc Reconstruction of a pathway of antigen processing and class II MHC peptide capture
    Catherine X Moss
    Division of Cell Biology and Immunology, School of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 26:2137-47. 2007
    ....
  39. doi request reprint Diverse regulatory roles for lysosomal proteases in the immune response
    Jeff D Colbert
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee, UK
    Eur J Immunol 39:2955-65. 2009
    ..We pay attention to reports demonstrating cathepsin activity outside of its canonical endosome/lysosome microenvironment...
  40. doi request reprint Asparagine endopeptidase is required for normal kidney physiology and homeostasis
    Gail Miller
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee, UK
    FASEB J 25:1606-17. 2011
    ..Thus, AEP is required for normal protein catabolism by PTCs, and its loss induces proliferative and other abnormalities in the murine kidney, at least in part through defective regulation of the EGF receptor...
  41. pmc Dendritic cell podosomes are protrusive and invade the extracellular matrix using metalloproteinase MMP-14
    Christian Gawden-Bone
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    J Cell Sci 123:1427-37. 2010
    ..We propose that podosomes mediate migration of dendritic cells through tissues by means of myosin-II-dependent protrusion coupled to MMP-14-dependent degradation and endocytosis...
  42. ncbi request reprint Class II MHC: sweetening the peptide only diet?
    Colin Watts
    Division of Cell Biology and Immunology, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom
    Cell 117:558-9. 2004
    ..In this issue of Cell, report an unexpected association of class II MHC molecules with processed zwitterionic polysaccharides from pathogenic bacteria. The complexes appear to modulate the T cell dependent pathology of abscess formation...
  43. ncbi request reprint Novel cell-permeable acyloxymethylketone inhibitors of asparaginyl endopeptidase
    Kylie Loak
    Medivir UK Ltd, Peterhouse Technology Park, 100 Fulbourn Road, Cambridge, CB1 9PT, UK
    Biol Chem 384:1239-46. 2003
    ..We show that this results in inhibition of AEP autoactivation and in perturbation of the processing and presentation of T cell epitopes from both tetanus toxin and myelin basic protein...
  44. ncbi request reprint Disulfide bonds in merozoite surface protein 1 of the malaria parasite impede efficient antigen processing and affect the in vivo antibody response
    Meike Hensmann
    Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London, GB
    Eur J Immunol 34:639-48. 2004
    ..Although the reduced form of MSP1(19) does not induce protective antibodies, our data suggest that inclusion of unfolded protein may improve the efficacy of MSP1(19) as a vaccine...
  45. ncbi request reprint Bm-CPI-2, a cystatin from Brugia malayi nematode parasites, differs from Caenorhabditis elegans cystatins in a specific site mediating inhibition of the antigen-processing enzyme AEP
    Janice Murray
    Institute of Immunology and Infection Research, Ashworth Laboratories, University of Edinburgh, West Mains Road, Scotland EH9 3JT, UK
    Mol Biochem Parasitol 139:197-203. 2005
    ..6) and Ce-CPI-2 (R01B10.1), both of which block cathepsins and neither of which possess the ability to block AEP. Thus, Brugia CPI-2 may have convergently evolved to inhibit an enzyme important only in the mammalian environment...
  46. ncbi request reprint Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification
    Tsing Cheng
    Department of Dermatology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    J Biol Chem 281:15893-9. 2006
    ....
  47. pmc Neuroprotective actions of PIKE-L by inhibition of SET proteolytic degradation by asparagine endopeptidase
    Zhixue Liu
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 615 Michael Street, Atlanta, GA 30322, USA
    Mol Cell 29:665-78. 2008
    ..Moreover, AEP also mediated stroke-provoked SET cleavage and cell death in brain. Thus, AEP might be one of the proteinases activated by acidosis triggering neuronal injury during neuroexcitotoxicity or ischemia...