Research Topics
Genomes and Genes
| Steve P WatsonSummaryAffiliation: University of Birmingham Country: UK Publications
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Detail Information
Publications
Studies on the actin-binding protein HS1 in plateletsSteven G Thomas
School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
BMC Cell Biol 8:46. 2007..The Arp2/3 complex is highly regulated by a number of actin-binding proteins including the haematopoietic-specific protein HS1 and its homologue cortactin. The present study investigates the role of HS1 in platelets using HS1-/- mice...
GPVI and integrin alphaIIb beta3 signaling in plateletsS P Watson
Division of Medical Sciences, Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
J Thromb Haemost 3:1752-62. 2005..The significance of these signaling cascades in platelet adhesion and platelet aggregation at arterial rates of shear is discussed...- Mapping the platelet proteome: a report of the ISTH Platelet Physiology SubcommitteeS P Watson
Centre for Cardiovascular Sciences, The Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham, UK
J Thromb Haemost 3:2098-101. 2005..It is recommended that proteomic information be combined with data from other experimental approaches to establish a database on protein expression and function in platelets... - Platelet activation by extracellular matrix proteins in haemostasis and thrombosisSteve P Watson
Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
Curr Pharm Des 15:1358-72. 2009..This review will focus on the role of the subendothelial matrix in haemostasis and thrombosis, highlighting its potential as a target for novel antithrombotics... 
GPVI and CLEC-2 in hemostasis and vascular integrityS P Watson
Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
J Thromb Haemost 8:1456-67. 2010....- Minimal regulation of platelet activity by PECAM-1Tarvinder S Dhanjal
Centre for Cardiovascular Sciences, Institute of Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
Platelets 18:56-67. 2007.... - Critical role of Src-Syk-PLC{gamma}2 signaling in megakaryocyte migration and thrombopoiesisAlexandra Mazharian
Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, United Kingdom
Blood 116:793-800. 2010..This correlation also holds for mice deficient in PLCgamma2. This study identifies a model in which integrin signaling via Src family kinases and Syk kinase to PLCgamma2 is required for MK spreading, migration, and platelet formation... - Phosphorylation of CLEC-2 is dependent on lipid rafts, actin polymerization, secondary mediators, and RacAlice Y Pollitt
Centre for Cardiovascular Sciences, Institute for Biomedical Research, The College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
Blood 115:2938-46. 2010..These results reveal a unique series of proximal events in CLEC-2 phosphorylation involving actin polymerization, secondary mediators, and Rac activation... - G6b-B inhibits constitutive and agonist-induced signaling by glycoprotein VI and CLEC-2Jun Mori
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, B15 2TT, UK
J Biol Chem 283:35419-27. 2008..We speculate that inhibition of constitutive signaling through Src and Syk tyrosine kinases by G6b-B may help to prevent unwanted platelet activation... - GPVI potentiation of platelet activation by thrombin and adhesion molecules independent of Src kinases and SykSascha C Hughan
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, UK
Arterioscler Thromb Vasc Biol 27:422-9. 2007..The present study investigates the role of Src and Syk tyrosine kinases in signaling by G-protein coupled and platelet adhesion receptors... - CLEC-2 activates Syk through dimerizationCraig E Hughes
Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
Blood 115:2947-55. 2010..This is a unique mode of activation of Syk by a single YxxL-containing receptor... - Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signalingSonia Severin
Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
J Biol Chem 286:4107-16. 2011.... - The tyrosine phosphatase CD148 is an essential positive regulator of platelet activation and thrombosisYotis A Senis
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, United Kingdom
Blood 113:4942-54. 2009..G protein-coupled receptor responses to thrombin and other agonists were also marginally reduced. These results highlight CD148 as a global regulator of platelet activation and a novel antithrombotic drug target... - CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for developmentBrenda A Finney
Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
Blood 119:1747-56. 2012.... - Identification of Tspan9 as a novel platelet tetraspanin and the collagen receptor GPVI as a component of tetraspanin microdomainsMajd B Protty
Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham B152TT, UK
Biochem J 417:391-400. 2009..These findings suggest a role for Tspan9 in regulating platelet function in concert with other platelet tetraspanins and their associated proteins... - Endothelial cell-borne platelet bridges selectively recruit monocytes in human and mouse models of vascular inflammationChristopher J Kuckleburg
Centre for Cardiovascular Sciences, Institute for Biomedical Research, The Medical School, The University of Birmingham, Birmingham B15 2TT, UK
Cardiovasc Res 91:134-41. 2011..In this study, we sought to expand on this knowledge using a complex coculture model of the diseased vessel wall... - Critical role of FcR gamma-chain, LAT, PLCgamma2 and thrombin in arteriolar thrombus formation upon mild, laser-induced endothelial injury in vivoNeena Kalia
Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, United Kingdom
Microcirculation 15:325-35. 2008..This study hypothesized that collagen exposure plays a critical role in thrombus formation in this model, which was tested by investigating contributions of FcR gamma-chain, LAT, PLCgamma2 and thrombin... - Dasatinib enhances megakaryocyte differentiation but inhibits platelet formationAlexandra Mazharian
Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, UK
Blood 117:5198-206. 2011..We propose that dasatinib causes thrombocytopenia as a consequence of ineffective thrombopoiesis, promoting MK differentiation but also impairing MK migration and proplatelet formation... - Adhesion of human and mouse platelets to collagen under shear: a unifying modelJocelyn M Auger
Centre for Cardiovascular Sciences, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham, UK
FASEB J 19:825-7. 2005..These observations form the basis of a unifying two-state model of platelet adhesion and aggregate formation on collagen that is conserved between human and mouse platelets... - The C-type lectin receptors CLEC-2 and Dectin-1, but not DC-SIGN, signal via a novel YXXL-dependent signaling cascadeGemma L J Fuller
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Birmingham B15 2TT, United Kingdom
J Biol Chem 282:12397-409. 2007..These results demonstrate that CLEC-2 and Dectin-1 signal through a single YXXL motif that requires the tandem SH2 domains of Syk but is only partially dependent on the SLP-76/BLNK family of adapters... - Vav family proteins are required for optimal regulation of PLCgamma2 by integrin alphaIIbbeta3Andrew C Pearce
Centre for Cardiovascular Sciences, Institute of Biomedical Research, Division of Medical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
Biochem J 401:753-61. 2007..These results demonstrate that Vav1 and Vav3 are required for optimal spreading and regulation of PLCgamma2 by integrin alphaIIbbeta3, but that their requirement is by-passed upon G-protein receptor activation... - Role of the p110delta PI 3-kinase in integrin and ITAM receptor signalling in plateletsYotis A Senis
Centre for Cardiovascular Sciences, Division of Medical Sciences, Institute of Biomedical Research, Wolfson Drive, The Medical School, University of Birmingham, Edgbaston, Birmingham, UK
Platelets 16:191-202. 2005..The more pronounced inhibitory effect of LY294002 and wortmannin indicates that other isoforms of PI 3-kinase play a more significant role in signalling by the two platelet glycoprotein receptors... - A novel role for PECAM-1 in megakaryocytokinesis and recovery of platelet counts in thrombocytopenic miceTarvinder S Dhanjal
Centre for Cardiovascular Sciences, Institute of Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Birmingham, United Kingdom
Blood 109:4237-44. 2007..This study identifies a novel role for PECAM-1 in regulating MK migration and thrombopoiesis... - Critical role for ERK1/2 in bone marrow and fetal liver-derived primary megakaryocyte differentiation, motility, and proplatelet formationAlexandra Mazharian
Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
Exp Hematol 37:1238-1249.e5. 2009.... - A major role for Scar/WAVE-1 downstream of GPVI in plateletsS D J Calaminus
Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
J Thromb Haemost 5:535-41. 2007..Rac mediates actin assembly via Scar/WAVE, a family of scaffolding proteins that direct actin reorganization by relaying signals from Rac to the Arp2/3 complex... - Proteomic analysis of integrin alphaIIbbeta3 outside-in signaling reveals Src-kinase-independent phosphorylation of Dok-1 and Dok-3 leading to SHIP-1 interactionsY A Senis
Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
J Thromb Haemost 7:1718-26. 2009..The aim of this study was to identify novel tyrosine phosphorylated signaling proteins downstream of alphaIIbbeta3, and explore their role in platelet signaling... - Rac1 is essential for platelet lamellipodia formation and aggregate stability under flowOwen J T McCarty
Centre for Cardiovascular Sciences, The Institute of Biomedical Research, School of Biosciences, University of Birmingham, United Kingdom
J Biol Chem 280:39474-84. 2005.... - Dynamic tyrosine kinase-regulated signaling and actin polymerisation mediate aggregate stability under shearJocelyn M Auger
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Birmingham, B15 2TT, UK
Arterioscler Thromb Vasc Biol 28:1499-504. 2008..We have investigated the role of these receptors and the actin cytoskeleton in maintaining aggregate stability under shear... - Molecular basis of platelet activation by an alphaIIbbeta3-CHAMPS peptideB Grygielska
Centre for Cardiovascular Sciences, Institute for Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Birmingham, UK
J Thromb Haemost 7:339-46. 2009.... - Differential regulation of adapter proteins Dok2 and Dok1 in platelets, leading to an association of Dok2 with integrin alphaIIbbeta3S C Hughan
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Birmingham, UK
J Thromb Haemost 5:387-94. 2007.... - von Willebrand factor mediates platelet spreading through glycoprotein Ib and alpha(IIb)beta3 in the presence of botrocetin and ristocetin, respectivelyO J T McCarty
Centre for Cardiovascular Sciences, University of Birmingham, Birmingham, UK
J Thromb Haemost 4:1367-78. 2006..To accomplish this, VWF binds to two distinct platelet receptors: glycoprotein (GP)Ib-IX-V and integrin alpha(IIb)beta3... - Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1BJun Mori
Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
Arterioscler Thromb Vasc Biol 32:2956-65. 2012..In the present study, we compared the relative contributions of these PTPs in platelet activation by the major glycoprotein, glycoprotein VI, α(IIb)β(3), and C-type lectin-like receptor 2 (CLEC-2)... - Regulation of proplatelet formation and platelet release by integrin alpha IIb beta3Mark K Larson
Centre for Cardiovascular Sciences, Institute for Biomedical Research, University of Birmingham, United Kingdom
Blood 108:1509-14. 2006..These results demonstrate that fibrinogen is able to regulate proplatelet formation via integrin alpha IIb beta3... - The novel Syk inhibitor R406 reveals mechanistic differences in the initiation of GPVI and CLEC-2 signaling in plateletsJ C Spalton
Centre for Cardiovascular Sciences, Institute for Biomedical Research, The College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, UK
J Thromb Haemost 7:1192-9. 2009..A recent study identified the novel small molecule R406 as a selective inhibitor of Syk... - Reference curves for aggregation and ATP secretion to aid diagnose of platelet-based bleeding disorders: effect of inhibition of ADP and thromboxane A(2) pathwaysBan B Dawood
Centre for Cardiovascular Sciences, Division of Medical Sciences, Institute of Biomedical Research, Wolfson Drive, The Medical School, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
Platelets 18:329-45. 2007..The present data-set are an invaluable resource to the clinical laboratory to aid evaluation of patients with suspected platelet-based bleeding disorders... - Isolation and characterization of cotiaractivase, a novel low molecular weight prothrombin activator from the venom of Bothrops cotiaraYotis A Senis
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Wolfson Drive, Edgbaston, Birmingham B15 2TT, UK
Biochim Biophys Acta 1764:863-71. 2006..Cotiaractivase is a unique low molecular weight snake venom prothrombin activator that likely belongs to the metalloproteinase family of proteins... - Vav1 and vav3 have critical but redundant roles in mediating platelet activation by collagenAndrew C Pearce
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK
J Biol Chem 279:53955-62. 2004..This is the first time that absolute redundancy between two protein isoforms has been observed with respect to the regulation of PLC gamma 2 in platelets... - Differential roles for the adapters Gads and LAT in platelet activation by GPVI and CLEC-2C E Hughes
Centre for Cardiovascular Sciences, Institute of Biomedical Research, Medical School, University of Birmingham, Birmingham, UK
J Thromb Haemost 6:2152-9. 2008..SLP-76 is constitutively associated with the adapter Gads in platelets, which also binds to tyrosine phosphorylated LAT, thereby providing a potential pathway of regulation of SLP-76... - Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreadingS D J Calaminus
Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
J Thromb Haemost 6:1944-52. 2008..In the present study, we report the identification of a novel actin-rich structure, termed an actin nodule, which appears prior to lamellipodia and stress fibre formation... - Collagen promotes sustained glycoprotein VI signaling in platelets and cell linesM G Tomlinson
Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
J Thromb Haemost 5:2274-83. 2007..Glycoprotein (GP)VI is the major signaling receptor for collagen on platelets and signals via the associated FcRgamma-chain, which has an immunoreceptor tyrosine-containing activation motif (ITAM)... - MyosinIIa contractility is required for maintenance of platelet structure during spreading on collagen and contributes to thrombus stabilityS D J Calaminus
Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
J Thromb Haemost 5:2136-45. 2007..MyosinIIa phosphorylation underlies platelet rounding and stress fiber formation... - Src family kinases are essential for primary aggregation by G(i) -coupled receptorsC A Nash
Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, UK
J Thromb Haemost 8:2273-82. 2010..In the present study, we demonstrate that both primary and secondary wave aggregation induced by adrenaline in plasma is blocked by two structurally distinct inhibitors of Src family kinases, dasatinib and PD0173952... - Evaluation of the role of platelet integrins in fibronectin-dependent spreading and adhesionO J T McCarty
Centre for Cardiovascular Sciences, The Institute of Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
J Thromb Haemost 2:1823-33. 2004..These results highlight a possible role for fibronectin in supporting thrombus formation... - c-Cbl negatively regulates platelet activation by glycoprotein VIJ M Auger
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, UK
J Thromb Haemost 1:2419-26. 2003..c-Cbl is tyrosine-phosphorylated in response to stimulation of GpVI, whereas phosphorylation of c-Cbl in thrombin-activated platelets is dependent on fibrinogen binding to the integrin GpIIb/IIIa... - Collagen or collagen-related peptide cause (Ca2+)i elevation and increased tyrosine phosphorylation in human megakaryocytesJ C Mountford
Department of Pharmacology, University of Oxford, UK
Thromb Haemost 82:1153-9. 1999.... - Evaluation of participants with suspected heritable platelet function disorders including recommendation and validation of a streamlined agonist panelBan B Dawood
Department of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
Blood 120:5041-9. 2012..We further demonstrate that most PFDs can be reliably diagnosed using a streamlined panel of key platelet agonists and specified concentrations suitable for testing in most clinical diagnostic laboratories... - Cellular pathology of atherosclerosis: smooth muscle cells promote adhesion of platelets to cocultured endothelial cellsSamantha P Tull
The Centre for Cardiovascular Sciences, The Medical School, The University of Birmingham, Edgbaston, Birmingham, United Kingdom
Circ Res 98:98-104. 2006..EC activated in this manner would therefore be capable of recruiting platelets in the low-shear environments that commonly exist at points of atheroma formation... - A product of their environment: do megakaryocytes rely on extracellular cues for proplatelet formation?Mark K Larson
Centre for Cardiovascular Sciences, Institute for Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Birmingham, UK B15 2TT, UK
Platelets 17:435-40. 2006..This review summarises this field and how these findings suggest a spatial and temporal regulation to ensure that platelets are produced in the correct location... - Selective impairment of platelet activation to collagen in the absence of GATA1Sascha C Hughan
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
Blood 105:4369-76. 2005..Our results are consistent with GATA1 regulating some but not all pathways of platelet activation, leading to an impairment of aggregate formation under flow, which cannot be attributed solely to the thrombocytopenia... - 50th anniversary of the discovery of ibuprofen: an interview with Dr Stewart AdamsGayle M Halford
Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
Platelets 23:415-22. 2012..In this article, Dr Adams gives his modest insight into the early stages and initial observations which led to this world-wide success... - Mice lacking the ITIM-containing receptor G6b-B exhibit macrothrombocytopenia and aberrant platelet functionAlexandra Mazharian
Centre of Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK
Sci Signal 5:ra78. 2012..Together, these findings establish G6b-B as a major inhibitory receptor regulating megakaryocyte activation, function, and platelet production... - CD31 is required on CD4+ T cells to promote T cell survival during Salmonella infectionEwan A Ross
Medical Research Council Centre for Immune Regulation, School of Immunity and Infection, Institute for Biomedical Research, University of Birmingham, Birmingham B15 2TT, United Kingdom
J Immunol 187:1553-65. 2011..This identifies a novel role for CD31 in regulating CD4 T cell homeostasis... - A germline mutation in BLOC1S3/reduced pigmentation causes a novel variant of Hermansky-Pudlak syndrome (HPS8)Neil V Morgan
Section of Medical and Molecular Genetics, Division of Medical Sciences, Institute of Biomedical Research, University of Birmingham, United Kingdom
Am J Hum Genet 78:160-6. 2006..These findings define a novel form of human HPS (HPS8) and extend genotype-phenotype correlations in HPS... - Glycoprotein VI oligomerization in cell lines and plateletsO Berlanga
Institute of Biomedical Research, Medical School, University of Birmingham, Edgbaston, Birmingham, UK
J Thromb Haemost 5:1026-33. 2007..Constitutive dimerization of GPVI has been proposed as being necessary for the interaction with collagen, although direct evidence of dimerization has not been reported in cell lines or platelets... - Characterization of Grb2-binding proteins in human platelets activated by Fc gamma RIIA cross-linkingA Robinson
University Department of Pharmacology, Oxford, UK
Blood 88:522-30. 1996..p38 and p63 may provide a docking site for Grb2, thereby linking Grb2 SH3-binding proteins SOS1, SLP-76, and p120 to downstream signalling events... - Identification of the phosphorylation sites of cytosolic phospholipase A2 in agonist-stimulated human platelets and HeLa cellsA G Borsch-Haubold
Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 273:4449-58. 1998.... - Glycoprotein VI is the collagen receptor in platelets which underlies tyrosine phosphorylation of the Fc receptor gamma-chainJ M Gibbins
Department of Pharmacology, University of Oxford, UK
FEBS Lett 413:255-9. 1997..These results support a model where GPVI couples collagen-stimulation of platelets to phosphorylation of the Fc receptor gamma-chain leading to activation of Syk and phospholipase Cgamma2... - The Fc receptor gamma-chain and the tyrosine kinase Syk are essential for activation of mouse platelets by collagenA Poole
Department of Pharmacology, University of Oxford, UK
EMBO J 16:2333-41. 1997..These observations provide the first direct evidence of an essential role for the immunoreceptor tyrosine-based activation motif (ITAM) in signalling by a non-immune receptor stimulus... - Activation of GPVI by collagen is regulated by alpha2beta1 and secondary mediatorsB T Atkinson
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
J Thromb Haemost 1:1278-87. 2003..Therefore we propose an important role of secondary mediators in collagen-induced signaling is the indirect regulation of GPVI signaling via activation of alpha(2)beta(1)... - Globular adiponectin induces platelet activation through the collagen receptor GPVI-Fc receptor gamma chain complexR Riba
Centre for Atherothrombosis Research, University of Bradford, Bradford, UK
J Thromb Haemost 6:1012-20. 2008..The adipocyte-derived cytokine, adiponectin (Ad), exerts potent vascular effects, although the direct effects of Ad on blood platelets are unclear... - A collagen-like peptide stimulates tyrosine phosphorylation of syk and phospholipase C gamma2 in platelets independent of the integrin alpha2beta1J Asselin
Department of Pharmacology, University of Oxford, UK
Blood 89:1235-42. 1997..The results show an alpha2beta1-independent pathway of platelet activation by CRP that involves phosphorylation of syk and PLC gamma2. This pathway appears to contribute to platelet activation by collagen... - Fetal hemorrhage and platelet dysfunction in SLP-76-deficient miceJ L Clements
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
J Clin Invest 103:19-25. 1999..These data provide one potential mechanism for the fetal hemorrhage observed in SLP-76-deficient mice and reveal that SLP-76 expression is required for optimal receptor-mediated signal transduction in platelets as well as T lymphocytes... - Tyrosine phosphorylation of SLP-76 is downstream of Syk following stimulation of the collagen receptor in plateletsB S Gross
Department of Pharmacology, Mansfield Road, Oxford University, Oxford OX1 3QT, United Kingdom
J Biol Chem 274:5963-71. 1999..This work identifies SLP-76 as an important adapter molecule that is regulated by Syk and lies upstream of SLAP-130 and PLC-gamma2 in CRP-stimulated platelets... - Evidence that phospholipase C-gamma2 interacts with SLP-76, Syk, Lyn, LAT and the Fc receptor gamma-chain after stimulation of the collagen receptor glycoprotein VI in human plateletsB S Gross
Department of Pharmacology, University of Oxford, UK
Eur J Biochem 263:612-23. 1999..These data indicate that the C-terminal SH2 domain of PLC-gamma2 is important for PLC-gamma2 regulation through possible interactions with SLP-76, Syk, Lyn, LAT and the FcR gamma-chain... - The snake venom toxin alboaggregin-A activates glycoprotein VIN Asazuma
Department of Pharmacology, University of Oxford, Mansfield Rd, Oxford, OX1 3QT, United Kingdom
Blood 97:3989-91. 2001..This may explain why responses to alboaggregin-A are distinct from those to von Willebrand factor-ristocetin. (Blood. 2001;97:3989-3991).. - Genetic and pharmacological analyses of involvement of Src-family, Syk and Btk tyrosine kinases in platelet shape change. Src-kinases mediate integrin alphaIIb beta3 inside-out signalling during shape changeM Bauer
, , Germany
Thromb Haemost 85:331-40. 2001.... - Fyn and Lyn phosphorylate the Fc receptor gamma chain downstream of glycoprotein VI in murine platelets, and Lyn regulates a novel feedback pathwayL S Quek
Department of Pharmacology, University of Oxford, United Kingdom
Blood 96:4246-53. 2000..Lyn plays an additional role in inhibiting platelet activation through an uncharacterized inhibitory pathway. (Blood. 2000;96:4246-4253).. - Evidence for two distinct epitopes within collagen for activation of murine plateletsV Schulte
Department of Molecular Oncology, General Surgery, Witten/Herdecke University, 42117 Wuppertal, Germany
J Biol Chem 276:364-8. 2001..The possibility that this response is mediated by a receptor that is not coupled to FcR gamma-chain is excluded on the grounds that aggregation is absent in platelets from FcR gamma-chain-deficient mice... - Interaction of linker for activation of T cells with multiple adapter proteins in platelets activated by the glycoprotein VI-selective ligand, convulxinN Asazuma
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, United Kingdom
J Biol Chem 275:33427-34. 2000.... - Phosphatidylinositol 3,4,5-trisphosphate regulates Ca(2+) entry via btk in platelets and megakaryocytes without increasing phospholipase C activityJ M Pasquet
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
EMBO J 19:2793-802. 2000..These results demonstrate a novel pathway of Ca(2+) entry that involves PI3,4,5P(3) and Btk, and which is independent of increased PLC activity... - Dichotomous regulation of myosin phosphorylation and shape change by Rho-kinase and calcium in intact human plateletsM Bauer
Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Klinikum Innenstadt, Universitat Munchen, Munchen, Germany
Blood 94:1665-72. 1999.... - Serine 727 phosphorylation and activation of cytosolic phospholipase A2 by MNK1-related protein kinasesY Hefner
Departments of Chemistry and Biochemistry, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 275:37542-51. 2000..The results suggest that MNK1 or a closely related kinase is responsible for in vivo phosphorylation of cPLA2 on Ser-727... - The endothelium and platelets in cardiovascular disease: potential targets for therapeutic interventionK T Tan
Haemostasis Thrombosis and Vascular Biology Unit, University Department of Medicine, City Hospital, Birmingham B18 7QH, England
Curr Med Chem Cardiovasc Hematol Agents 2:169-78. 2004..This review focuses on the recent advances in our understanding of the interaction(s) between platelets and endothelium and how this knowledge could be manipulated for therapeutic gain... - The use of snake venom toxins as tools to study platelet receptors for collagen and von Willebrand factorR K Andrews
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK
Haemostasis 31:155-72. 2001..This review discusses the substantial contribution that venom proteins have made to our understanding of the role of these two adhesive proteins and their receptors (excluding GPIIb-IIIa) in platelet regulation...