Steve P Watson

Summary

Affiliation: University of Birmingham
Country: UK

Publications

  1. doi request reprint GPVI and CLEC-2 in hemostasis and vascular integrity
    S P Watson
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
    J Thromb Haemost 8:1456-67. 2010
  2. doi request reprint Platelets in lymph vessel development and integrity
    Steve P Watson
    Centre for Cardiovascular Sciences, The College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
    Adv Anat Embryol Cell Biol 214:93-105. 2014
  3. doi request reprint Genotyping and phenotyping of platelet function disorders
    S P Watson
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
    J Thromb Haemost 11:351-63. 2013
  4. pmc Studies on the actin-binding protein HS1 in platelets
    Steven G Thomas
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    BMC Cell Biol 8:46. 2007
  5. ncbi request reprint Platelet activation by extracellular matrix proteins in haemostasis and thrombosis
    Steve P Watson
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
    Curr Pharm Des 15:1358-72. 2009
  6. ncbi request reprint GPVI and integrin alphaIIb beta3 signaling in platelets
    S P Watson
    Division of Medical Sciences, Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 3:1752-62. 2005
  7. ncbi request reprint Mapping the platelet proteome: a report of the ISTH Platelet Physiology Subcommittee
    S P Watson
    Centre for Cardiovascular Sciences, The Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham, UK
    J Thromb Haemost 3:2098-101. 2005
  8. pmc Minimal regulation of platelet activity by PECAM-1
    Tarvinder S Dhanjal
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
    Platelets 18:56-67. 2007
  9. doi request reprint Phosphorylation of CLEC-2 is dependent on lipid rafts, actin polymerization, secondary mediators, and Rac
    Alice Y Pollitt
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, The College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
    Blood 115:2938-46. 2010
  10. doi request reprint Critical role of Src-Syk-PLC{gamma}2 signaling in megakaryocyte migration and thrombopoiesis
    Alexandra Mazharian
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, United Kingdom
    Blood 116:793-800. 2010

Detail Information

Publications81

  1. doi request reprint GPVI and CLEC-2 in hemostasis and vascular integrity
    S P Watson
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
    J Thromb Haemost 8:1456-67. 2010
    ....
  2. doi request reprint Platelets in lymph vessel development and integrity
    Steve P Watson
    Centre for Cardiovascular Sciences, The College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
    Adv Anat Embryol Cell Biol 214:93-105. 2014
    ..In this review, we describe the events that led to the identification of this novel role of platelets and discuss possible molecular mechanisms and the physiological and pathophysiological significance. ..
  3. doi request reprint Genotyping and phenotyping of platelet function disorders
    S P Watson
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
    J Thromb Haemost 11:351-63. 2013
    ..Mucocutaneous bleeding is a complex trait, and this has important implications for NGS in the assessment of a PFD...
  4. pmc Studies on the actin-binding protein HS1 in platelets
    Steven G Thomas
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    BMC Cell Biol 8:46. 2007
    ..The Arp2/3 complex is highly regulated by a number of actin-binding proteins including the haematopoietic-specific protein HS1 and its homologue cortactin. The present study investigates the role of HS1 in platelets using HS1-/- mice...
  5. ncbi request reprint Platelet activation by extracellular matrix proteins in haemostasis and thrombosis
    Steve P Watson
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
    Curr Pharm Des 15:1358-72. 2009
    ..This review will focus on the role of the subendothelial matrix in haemostasis and thrombosis, highlighting its potential as a target for novel antithrombotics...
  6. ncbi request reprint GPVI and integrin alphaIIb beta3 signaling in platelets
    S P Watson
    Division of Medical Sciences, Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 3:1752-62. 2005
    ..The significance of these signaling cascades in platelet adhesion and platelet aggregation at arterial rates of shear is discussed...
  7. ncbi request reprint Mapping the platelet proteome: a report of the ISTH Platelet Physiology Subcommittee
    S P Watson
    Centre for Cardiovascular Sciences, The Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham, UK
    J Thromb Haemost 3:2098-101. 2005
    ..It is recommended that proteomic information be combined with data from other experimental approaches to establish a database on protein expression and function in platelets...
  8. pmc Minimal regulation of platelet activity by PECAM-1
    Tarvinder S Dhanjal
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
    Platelets 18:56-67. 2007
    ....
  9. doi request reprint Phosphorylation of CLEC-2 is dependent on lipid rafts, actin polymerization, secondary mediators, and Rac
    Alice Y Pollitt
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, The College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
    Blood 115:2938-46. 2010
    ..These results reveal a unique series of proximal events in CLEC-2 phosphorylation involving actin polymerization, secondary mediators, and Rac activation...
  10. doi request reprint Critical role of Src-Syk-PLC{gamma}2 signaling in megakaryocyte migration and thrombopoiesis
    Alexandra Mazharian
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, United Kingdom
    Blood 116:793-800. 2010
    ..This correlation also holds for mice deficient in PLCgamma2. This study identifies a model in which integrin signaling via Src family kinases and Syk kinase to PLCgamma2 is required for MK spreading, migration, and platelet formation...
  11. pmc G6b-B inhibits constitutive and agonist-induced signaling by glycoprotein VI and CLEC-2
    Jun Mori
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, B15 2TT, UK
    J Biol Chem 283:35419-27. 2008
    ..We speculate that inhibition of constitutive signaling through Src and Syk tyrosine kinases by G6b-B may help to prevent unwanted platelet activation...
  12. pmc GPVI potentiation of platelet activation by thrombin and adhesion molecules independent of Src kinases and Syk
    Sascha C Hughan
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, UK
    Arterioscler Thromb Vasc Biol 27:422-9. 2007
    ..The present study investigates the role of Src and Syk tyrosine kinases in signaling by G-protein coupled and platelet adhesion receptors...
  13. doi request reprint CLEC-2 activates Syk through dimerization
    Craig E Hughes
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
    Blood 115:2947-55. 2010
    ..This is a unique mode of activation of Syk by a single YxxL-containing receptor...
  14. pmc The physiological and pathophysiological roles of platelet CLEC-2
    Leyre Navarro-Nuñez
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK
    Thromb Haemost 109:991-8. 2013
    ..This review summarises the current understanding of the molecular pathways regulating CLEC-2 and podoplanin function and suggests other physiological and pathological processes where this molecular interaction might exert crucial roles...
  15. pmc Evaluation of participants with suspected heritable platelet function disorders including recommendation and validation of a streamlined agonist panel
    Ban B Dawood
    Department of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
    Blood 120:5041-9. 2012
    ..We further demonstrate that most PFDs can be reliably diagnosed using a streamlined panel of key platelet agonists and specified concentrations suitable for testing in most clinical diagnostic laboratories...
  16. pmc CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for development
    Brenda A Finney
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
    Blood 119:1747-56. 2012
    ....
  17. pmc Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signaling
    Sonia Severin
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
    J Biol Chem 286:4107-16. 2011
    ....
  18. pmc The tyrosine phosphatase CD148 is an essential positive regulator of platelet activation and thrombosis
    Yotis A Senis
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, United Kingdom
    Blood 113:4942-54. 2009
    ..G protein-coupled receptor responses to thrombin and other agonists were also marginally reduced. These results highlight CD148 as a global regulator of platelet activation and a novel antithrombotic drug target...
  19. pmc Megakaryocyte-specific deletion of the protein-tyrosine phosphatases Shp1 and Shp2 causes abnormal megakaryocyte development, platelet production, and function
    Alexandra Mazharian
    Centre for Cardiovascular Sciences, School of Clinical and Experimental Medicine, Institute of Biomedical Research, University of Birmingham, United Kingdom
    Blood 121:4205-20. 2013
    ..This study establishes Shp1 and Shp2 as major regulators of megakaryocyte development, platelet production, and function...
  20. pmc Critical Role for an acidic amino acid region in platelet signaling by the HemITAM (hemi-immunoreceptor tyrosine-based activation motif) containing receptor CLEC-2 (C-type lectin receptor-2)
    Craig E Hughes
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, The College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Biol Chem 288:5127-35. 2013
    ..These results further emphasize the distinct nature of the proximal events in signaling by hemITAM relative to ITAM receptors...
  21. doi request reprint Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1B
    Jun Mori
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
    Arterioscler Thromb Vasc Biol 32:2956-65. 2012
    ..In the present study, we compared the relative contributions of these PTPs in platelet activation by the major glycoprotein, glycoprotein VI, α(IIb)β(3), and C-type lectin-like receptor 2 (CLEC-2)...
  22. doi request reprint Mice lacking the ITIM-containing receptor G6b-B exhibit macrothrombocytopenia and aberrant platelet function
    Alexandra Mazharian
    Centre of Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK
    Sci Signal 5:ra78. 2012
    ..Together, these findings establish G6b-B as a major inhibitory receptor regulating megakaryocyte activation, function, and platelet production...
  23. pmc Identification of Tspan9 as a novel platelet tetraspanin and the collagen receptor GPVI as a component of tetraspanin microdomains
    Majd B Protty
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham B152TT, UK
    Biochem J 417:391-400. 2009
    ..These findings suggest a role for Tspan9 in regulating platelet function in concert with other platelet tetraspanins and their associated proteins...
  24. doi request reprint Critical role of FcR gamma-chain, LAT, PLCgamma2 and thrombin in arteriolar thrombus formation upon mild, laser-induced endothelial injury in vivo
    Neena Kalia
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, United Kingdom
    Microcirculation 15:325-35. 2008
    ..This study hypothesized that collagen exposure plays a critical role in thrombus formation in this model, which was tested by investigating contributions of FcR gamma-chain, LAT, PLCgamma2 and thrombin...
  25. doi request reprint Endothelial cell-borne platelet bridges selectively recruit monocytes in human and mouse models of vascular inflammation
    Christopher J Kuckleburg
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, The Medical School, The University of Birmingham, Birmingham B15 2TT, UK
    Cardiovasc Res 91:134-41. 2011
    ..In this study, we sought to expand on this knowledge using a complex coculture model of the diseased vessel wall...
  26. pmc Dasatinib enhances megakaryocyte differentiation but inhibits platelet formation
    Alexandra Mazharian
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, UK
    Blood 117:5198-206. 2011
    ..We propose that dasatinib causes thrombocytopenia as a consequence of ineffective thrombopoiesis, promoting MK differentiation but also impairing MK migration and proplatelet formation...
  27. ncbi request reprint Adhesion of human and mouse platelets to collagen under shear: a unifying model
    Jocelyn M Auger
    Centre for Cardiovascular Sciences, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham, UK
    FASEB J 19:825-7. 2005
    ..These observations form the basis of a unifying two-state model of platelet adhesion and aggregate formation on collagen that is conserved between human and mouse platelets...
  28. pmc Role of the p110delta PI 3-kinase in integrin and ITAM receptor signalling in platelets
    Yotis A Senis
    Centre for Cardiovascular Sciences, Division of Medical Sciences, Institute of Biomedical Research, Wolfson Drive, The Medical School, University of Birmingham, Edgbaston, Birmingham, UK
    Platelets 16:191-202. 2005
    ..The more pronounced inhibitory effect of LY294002 and wortmannin indicates that other isoforms of PI 3-kinase play a more significant role in signalling by the two platelet glycoprotein receptors...
  29. pmc Vav family proteins are required for optimal regulation of PLCgamma2 by integrin alphaIIbbeta3
    Andrew C Pearce
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, Division of Medical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Biochem J 401:753-61. 2007
    ..These results demonstrate that Vav1 and Vav3 are required for optimal spreading and regulation of PLCgamma2 by integrin alphaIIbbeta3, but that their requirement is by-passed upon G-protein receptor activation...
  30. pmc Critical role for ERK1/2 in bone marrow and fetal liver-derived primary megakaryocyte differentiation, motility, and proplatelet formation
    Alexandra Mazharian
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
    Exp Hematol 37:1238-1249.e5. 2009
    ....
  31. pmc The C-type lectin receptors CLEC-2 and Dectin-1, but not DC-SIGN, signal via a novel YXXL-dependent signaling cascade
    Gemma L J Fuller
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Birmingham B15 2TT, United Kingdom
    J Biol Chem 282:12397-409. 2007
    ..These results demonstrate that CLEC-2 and Dectin-1 signal through a single YXXL motif that requires the tandem SH2 domains of Syk but is only partially dependent on the SLP-76/BLNK family of adapters...
  32. ncbi request reprint A novel role for PECAM-1 in megakaryocytokinesis and recovery of platelet counts in thrombocytopenic mice
    Tarvinder S Dhanjal
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Birmingham, United Kingdom
    Blood 109:4237-44. 2007
    ..This study identifies a novel role for PECAM-1 in regulating MK migration and thrombopoiesis...
  33. pmc A major role for Scar/WAVE-1 downstream of GPVI in platelets
    S D J Calaminus
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 5:535-41. 2007
    ..Rac mediates actin assembly via Scar/WAVE, a family of scaffolding proteins that direct actin reorganization by relaying signals from Rac to the Arp2/3 complex...
  34. doi request reprint Proteomic analysis of integrin alphaIIbbeta3 outside-in signaling reveals Src-kinase-independent phosphorylation of Dok-1 and Dok-3 leading to SHIP-1 interactions
    Y A Senis
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
    J Thromb Haemost 7:1718-26. 2009
    ..The aim of this study was to identify novel tyrosine phosphorylated signaling proteins downstream of alphaIIbbeta3, and explore their role in platelet signaling...
  35. pmc Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow
    Owen J T McCarty
    Centre for Cardiovascular Sciences, The Institute of Biomedical Research, School of Biosciences, University of Birmingham, United Kingdom
    J Biol Chem 280:39474-84. 2005
    ....
  36. doi request reprint Dynamic tyrosine kinase-regulated signaling and actin polymerisation mediate aggregate stability under shear
    Jocelyn M Auger
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Birmingham, B15 2TT, UK
    Arterioscler Thromb Vasc Biol 28:1499-504. 2008
    ..We have investigated the role of these receptors and the actin cytoskeleton in maintaining aggregate stability under shear...
  37. ncbi request reprint von Willebrand factor mediates platelet spreading through glycoprotein Ib and alpha(IIb)beta3 in the presence of botrocetin and ristocetin, respectively
    O J T McCarty
    Centre for Cardiovascular Sciences, University of Birmingham, Birmingham, UK
    J Thromb Haemost 4:1367-78. 2006
    ..To accomplish this, VWF binds to two distinct platelet receptors: glycoprotein (GP)Ib-IX-V and integrin alpha(IIb)beta3...
  38. doi request reprint Molecular basis of platelet activation by an alphaIIbbeta3-CHAMPS peptide
    B Grygielska
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 7:339-46. 2009
    ....
  39. ncbi request reprint Differential regulation of adapter proteins Dok2 and Dok1 in platelets, leading to an association of Dok2 with integrin alphaIIbbeta3
    S C Hughan
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Birmingham, UK
    J Thromb Haemost 5:387-94. 2007
    ....
  40. doi request reprint Growth factor receptor-bound protein 2 contributes to (hem)immunoreceptor tyrosine-based activation motif-mediated signaling in platelets
    Sebastian Dütting
    From the Department of Experimental Biomedicine, University Hospital Würzburg S D, T V, M M, S S, B N and Rudolf Virchow Center for Experimental Biomedicine S D, T V, C M S, H M H, B N, University of Wurzburg, Wurzburg, Germany Centre for Cardiovascular Sciences, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom S K W, C E H, S P W and Department of Biology, Division of Genetics, University of Erlangen, Erlangen, Germany J A A, D R, L N
    Circ Res 114:444-53. 2014
    ..Growth factor receptor-bound protein 2 (Grb2) is a ubiquitously expressed adapter molecule involved in signaling processes of numerous receptors in different cell types, but its function in platelets and MKs is unknown...
  41. ncbi request reprint Regulation of proplatelet formation and platelet release by integrin alpha IIb beta3
    Mark K Larson
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, University of Birmingham, United Kingdom
    Blood 108:1509-14. 2006
    ..These results demonstrate that fibrinogen is able to regulate proplatelet formation via integrin alpha IIb beta3...
  42. doi request reprint The novel Syk inhibitor R406 reveals mechanistic differences in the initiation of GPVI and CLEC-2 signaling in platelets
    J C Spalton
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, The College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, UK
    J Thromb Haemost 7:1192-9. 2009
    ..A recent study identified the novel small molecule R406 as a selective inhibitor of Syk...
  43. ncbi request reprint Reference curves for aggregation and ATP secretion to aid diagnose of platelet-based bleeding disorders: effect of inhibition of ADP and thromboxane A(2) pathways
    Ban B Dawood
    Centre for Cardiovascular Sciences, Division of Medical Sciences, Institute of Biomedical Research, Wolfson Drive, The Medical School, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Platelets 18:329-45. 2007
    ..The present data-set are an invaluable resource to the clinical laboratory to aid evaluation of patients with suspected platelet-based bleeding disorders...
  44. ncbi request reprint Isolation and characterization of cotiaractivase, a novel low molecular weight prothrombin activator from the venom of Bothrops cotiara
    Yotis A Senis
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Wolfson Drive, Edgbaston, Birmingham B15 2TT, UK
    Biochim Biophys Acta 1764:863-71. 2006
    ..Cotiaractivase is a unique low molecular weight snake venom prothrombin activator that likely belongs to the metalloproteinase family of proteins...
  45. ncbi request reprint Vav1 and vav3 have critical but redundant roles in mediating platelet activation by collagen
    Andrew C Pearce
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK
    J Biol Chem 279:53955-62. 2004
    ..This is the first time that absolute redundancy between two protein isoforms has been observed with respect to the regulation of PLC gamma 2 in platelets...
  46. pmc Differential roles for the adapters Gads and LAT in platelet activation by GPVI and CLEC-2
    C E Hughes
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 6:2152-9. 2008
    ..SLP-76 is constitutively associated with the adapter Gads in platelets, which also binds to tyrosine phosphorylated LAT, thereby providing a potential pathway of regulation of SLP-76...
  47. doi request reprint Src family kinases are essential for primary aggregation by G(i) -coupled receptors
    C A Nash
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, UK
    J Thromb Haemost 8:2273-82. 2010
    ..In the present study, we demonstrate that both primary and secondary wave aggregation induced by adrenaline in plasma is blocked by two structurally distinct inhibitors of Src family kinases, dasatinib and PD0173952...
  48. ncbi request reprint c-Cbl negatively regulates platelet activation by glycoprotein VI
    J M Auger
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, UK
    J Thromb Haemost 1:2419-26. 2003
    ..c-Cbl is tyrosine-phosphorylated in response to stimulation of GpVI, whereas phosphorylation of c-Cbl in thrombin-activated platelets is dependent on fibrinogen binding to the integrin GpIIb/IIIa...
  49. ncbi request reprint Collagen promotes sustained glycoprotein VI signaling in platelets and cell lines
    M G Tomlinson
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 5:2274-83. 2007
    ..Glycoprotein (GP)VI is the major signaling receptor for collagen on platelets and signals via the associated FcRgamma-chain, which has an immunoreceptor tyrosine-containing activation motif (ITAM)...
  50. ncbi request reprint MyosinIIa contractility is required for maintenance of platelet structure during spreading on collagen and contributes to thrombus stability
    S D J Calaminus
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 5:2136-45. 2007
    ..MyosinIIa phosphorylation underlies platelet rounding and stress fiber formation...
  51. ncbi request reprint Collagen or collagen-related peptide cause (Ca2+)i elevation and increased tyrosine phosphorylation in human megakaryocytes
    J C Mountford
    Department of Pharmacology, University of Oxford, UK
    Thromb Haemost 82:1153-9. 1999
    ....
  52. doi request reprint Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading
    S D J Calaminus
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 6:1944-52. 2008
    ..In the present study, we report the identification of a novel actin-rich structure, termed an actin nodule, which appears prior to lamellipodia and stress fibre formation...
  53. ncbi request reprint Evaluation of the role of platelet integrins in fibronectin-dependent spreading and adhesion
    O J T McCarty
    Centre for Cardiovascular Sciences, The Institute of Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    J Thromb Haemost 2:1823-33. 2004
    ..These results highlight a possible role for fibronectin in supporting thrombus formation...
  54. doi request reprint Characterization of multiple platelet activation pathways in patients with bleeding as a high-throughput screening option: use of 96-well Optimul assay
    Marie Lordkipanidzé
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    Blood 123:e11-22. 2014
    ..This trial was registered at www.isrctn.org as #ISRCTN 77951167. ..
  55. ncbi request reprint Cellular pathology of atherosclerosis: smooth muscle cells promote adhesion of platelets to cocultured endothelial cells
    Samantha P Tull
    The Centre for Cardiovascular Sciences, The Medical School, The University of Birmingham, Edgbaston, Birmingham, United Kingdom
    Circ Res 98:98-104. 2006
    ..EC activated in this manner would therefore be capable of recruiting platelets in the low-shear environments that commonly exist at points of atheroma formation...
  56. ncbi request reprint Selective impairment of platelet activation to collagen in the absence of GATA1
    Sascha C Hughan
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    Blood 105:4369-76. 2005
    ..Our results are consistent with GATA1 regulating some but not all pathways of platelet activation, leading to an impairment of aggregate formation under flow, which cannot be attributed solely to the thrombocytopenia...
  57. ncbi request reprint A product of their environment: do megakaryocytes rely on extracellular cues for proplatelet formation?
    Mark K Larson
    Centre for Cardiovascular Sciences, Institute for Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Birmingham, UK B15 2TT, UK
    Platelets 17:435-40. 2006
    ..This review summarises this field and how these findings suggest a spatial and temporal regulation to ensure that platelets are produced in the correct location...
  58. pmc A germline mutation in BLOC1S3/reduced pigmentation causes a novel variant of Hermansky-Pudlak syndrome (HPS8)
    Neil V Morgan
    Section of Medical and Molecular Genetics, Division of Medical Sciences, Institute of Biomedical Research, University of Birmingham, United Kingdom
    Am J Hum Genet 78:160-6. 2006
    ..These findings define a novel form of human HPS (HPS8) and extend genotype-phenotype correlations in HPS...
  59. doi request reprint 50th anniversary of the discovery of ibuprofen: an interview with Dr Stewart Adams
    Gayle M Halford
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    Platelets 23:415-22. 2012
    ..In this article, Dr Adams gives his modest insight into the early stages and initial observations which led to this world-wide success...
  60. pmc CD31 is required on CD4+ T cells to promote T cell survival during Salmonella infection
    Ewan A Ross
    Medical Research Council Centre for Immune Regulation, School of Immunity and Infection, Institute for Biomedical Research, University of Birmingham, Birmingham B15 2TT, United Kingdom
    J Immunol 187:1553-65. 2011
    ..This identifies a novel role for CD31 in regulating CD4 T cell homeostasis...
  61. pmc Glycoprotein VI oligomerization in cell lines and platelets
    O Berlanga
    Institute of Biomedical Research, Medical School, University of Birmingham, Edgbaston, Birmingham, UK
    J Thromb Haemost 5:1026-33. 2007
    ..Constitutive dimerization of GPVI has been proposed as being necessary for the interaction with collagen, although direct evidence of dimerization has not been reported in cell lines or platelets...
  62. ncbi request reprint A collagen-like peptide stimulates tyrosine phosphorylation of syk and phospholipase C gamma2 in platelets independent of the integrin alpha2beta1
    J Asselin
    Department of Pharmacology, University of Oxford, UK
    Blood 89:1235-42. 1997
    ..The results show an alpha2beta1-independent pathway of platelet activation by CRP that involves phosphorylation of syk and PLC gamma2. This pathway appears to contribute to platelet activation by collagen...
  63. ncbi request reprint Characterization of Grb2-binding proteins in human platelets activated by Fc gamma RIIA cross-linking
    A Robinson
    University Department of Pharmacology, Oxford, UK
    Blood 88:522-30. 1996
    ..p38 and p63 may provide a docking site for Grb2, thereby linking Grb2 SH3-binding proteins SOS1, SLP-76, and p120 to downstream signalling events...
  64. ncbi request reprint Dichotomous regulation of myosin phosphorylation and shape change by Rho-kinase and calcium in intact human platelets
    M Bauer
    Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Klinikum Innenstadt, Universitat Munchen, Munchen, Germany
    Blood 94:1665-72. 1999
    ....
  65. ncbi request reprint Evidence that phospholipase C-gamma2 interacts with SLP-76, Syk, Lyn, LAT and the Fc receptor gamma-chain after stimulation of the collagen receptor glycoprotein VI in human platelets
    B S Gross
    Department of Pharmacology, University of Oxford, UK
    Eur J Biochem 263:612-23. 1999
    ..These data indicate that the C-terminal SH2 domain of PLC-gamma2 is important for PLC-gamma2 regulation through possible interactions with SLP-76, Syk, Lyn, LAT and the FcR gamma-chain...
  66. ncbi request reprint Tyrosine phosphorylation of SLP-76 is downstream of Syk following stimulation of the collagen receptor in platelets
    B S Gross
    Department of Pharmacology, Mansfield Road, Oxford University, Oxford OX1 3QT, United Kingdom
    J Biol Chem 274:5963-71. 1999
    ..This work identifies SLP-76 as an important adapter molecule that is regulated by Syk and lies upstream of SLAP-130 and PLC-gamma2 in CRP-stimulated platelets...
  67. pmc Fetal hemorrhage and platelet dysfunction in SLP-76-deficient mice
    J L Clements
    Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Clin Invest 103:19-25. 1999
    ..These data provide one potential mechanism for the fetal hemorrhage observed in SLP-76-deficient mice and reveal that SLP-76 expression is required for optimal receptor-mediated signal transduction in platelets as well as T lymphocytes...
  68. ncbi request reprint Glycoprotein VI is the collagen receptor in platelets which underlies tyrosine phosphorylation of the Fc receptor gamma-chain
    J M Gibbins
    Department of Pharmacology, University of Oxford, UK
    FEBS Lett 413:255-9. 1997
    ..These results support a model where GPVI couples collagen-stimulation of platelets to phosphorylation of the Fc receptor gamma-chain leading to activation of Syk and phospholipase Cgamma2...
  69. pmc The Fc receptor gamma-chain and the tyrosine kinase Syk are essential for activation of mouse platelets by collagen
    A Poole
    Department of Pharmacology, University of Oxford, UK
    EMBO J 16:2333-41. 1997
    ..These observations provide the first direct evidence of an essential role for the immunoreceptor tyrosine-based activation motif (ITAM) in signalling by a non-immune receptor stimulus...
  70. ncbi request reprint Identification of the phosphorylation sites of cytosolic phospholipase A2 in agonist-stimulated human platelets and HeLa cells
    A G Borsch-Haubold
    Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 273:4449-58. 1998
    ....
  71. pmc Phosphatidylinositol 3,4,5-trisphosphate regulates Ca(2+) entry via btk in platelets and megakaryocytes without increasing phospholipase C activity
    J M Pasquet
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    EMBO J 19:2793-802. 2000
    ..These results demonstrate a novel pathway of Ca(2+) entry that involves PI3,4,5P(3) and Btk, and which is independent of increased PLC activity...
  72. ncbi request reprint Interaction of linker for activation of T cells with multiple adapter proteins in platelets activated by the glycoprotein VI-selective ligand, convulxin
    N Asazuma
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, United Kingdom
    J Biol Chem 275:33427-34. 2000
    ....
  73. ncbi request reprint Activation of GPVI by collagen is regulated by alpha2beta1 and secondary mediators
    B T Atkinson
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    J Thromb Haemost 1:1278-87. 2003
    ..Therefore we propose an important role of secondary mediators in collagen-induced signaling is the indirect regulation of GPVI signaling via activation of alpha(2)beta(1)...
  74. ncbi request reprint The snake venom toxin alboaggregin-A activates glycoprotein VI
    N Asazuma
    Department of Pharmacology, University of Oxford, Mansfield Rd, Oxford, OX1 3QT, United Kingdom
    Blood 97:3989-91. 2001
    ..This may explain why responses to alboaggregin-A are distinct from those to von Willebrand factor-ristocetin. (Blood. 2001;97:3989-3991)..
  75. ncbi request reprint Genetic and pharmacological analyses of involvement of Src-family, Syk and Btk tyrosine kinases in platelet shape change. Src-kinases mediate integrin alphaIIb beta3 inside-out signalling during shape change
    M Bauer
    , , Germany
    Thromb Haemost 85:331-40. 2001
    ....
  76. ncbi request reprint Fyn and Lyn phosphorylate the Fc receptor gamma chain downstream of glycoprotein VI in murine platelets, and Lyn regulates a novel feedback pathway
    L S Quek
    Department of Pharmacology, University of Oxford, United Kingdom
    Blood 96:4246-53. 2000
    ..Lyn plays an additional role in inhibiting platelet activation through an uncharacterized inhibitory pathway. (Blood. 2000;96:4246-4253)..
  77. ncbi request reprint Evidence for two distinct epitopes within collagen for activation of murine platelets
    V Schulte
    Department of Molecular Oncology, General Surgery, Witten/Herdecke University, 42117 Wuppertal, Germany
    J Biol Chem 276:364-8. 2001
    ..The possibility that this response is mediated by a receptor that is not coupled to FcR gamma-chain is excluded on the grounds that aggregation is absent in platelets from FcR gamma-chain-deficient mice...
  78. ncbi request reprint Serine 727 phosphorylation and activation of cytosolic phospholipase A2 by MNK1-related protein kinases
    Y Hefner
    Departments of Chemistry and Biochemistry, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 275:37542-51. 2000
    ..The results suggest that MNK1 or a closely related kinase is responsible for in vivo phosphorylation of cPLA2 on Ser-727...
  79. doi request reprint Globular adiponectin induces platelet activation through the collagen receptor GPVI-Fc receptor gamma chain complex
    R Riba
    Centre for Atherothrombosis Research, University of Bradford, Bradford, UK
    J Thromb Haemost 6:1012-20. 2008
    ..The adipocyte-derived cytokine, adiponectin (Ad), exerts potent vascular effects, although the direct effects of Ad on blood platelets are unclear...
  80. ncbi request reprint The use of snake venom toxins as tools to study platelet receptors for collagen and von Willebrand factor
    R K Andrews
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK
    Haemostasis 31:155-72. 2001
    ..This review discusses the substantial contribution that venom proteins have made to our understanding of the role of these two adhesive proteins and their receptors (excluding GPIIb-IIIa) in platelet regulation...
  81. ncbi request reprint The endothelium and platelets in cardiovascular disease: potential targets for therapeutic intervention
    K T Tan
    Haemostasis Thrombosis and Vascular Biology Unit, University Department of Medicine, City Hospital, Birmingham B18 7QH, England
    Curr Med Chem Cardiovasc Hematol Agents 2:169-78. 2004
    ..This review focuses on the recent advances in our understanding of the interaction(s) between platelets and endothelium and how this knowledge could be manipulated for therapeutic gain...