T R Walsh

Summary

Affiliation: University of Bristol
Country: UK

Publications

  1. pmc Emergence of the extended-spectrum beta-lactamase GES-1 in a Pseudomonas aeruginosa strain from Brazil: report from the SENTRY antimicrobial surveillance program
    Mariana Castanheira
    Antimicrob Agents Chemother 48:2344-5. 2004
  2. ncbi request reprint Emerging metallo-beta-lactamase-mediated resistances: a summary report from the worldwide SENTRY antimicrobial surveillance program
    Thomas R Fritsche
    Jones Microbiology Institute Laboratories, North Liberty, IA 52317, USA
    Clin Infect Dis 41:S276-8. 2005
  3. doi request reprint Clinically significant carbapenemases: an update
    Timothy R Walsh
    Department of Medical Microbiology, School of Medical Sciences, Cardiff University, Heath Park Hospital, Cardiff, UK
    Curr Opin Infect Dis 21:367-71. 2008
  4. pmc Evaluation of a new Etest for detecting metallo-beta-lactamases in routine clinical testing
    Timothy R Walsh
    University of Bristol, United Kingdom
    J Clin Microbiol 40:2755-9. 2002
  5. pmc Nucleotide and amino acid sequences of the metallo-beta-lactamase, ImiS, from Aeromonas veronii bv. sobria
    T R Walsh
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Microbiology and Pathology, Medical School, University of Bristol, United Kingdom
    Antimicrob Agents Chemother 42:436-9. 1998
  6. pmc Metallo-beta-lactamases: the quiet before the storm?
    Timothy R Walsh
    Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    Clin Microbiol Rev 18:306-25. 2005
  7. ncbi request reprint Characterization of a carbapenemase-producing clinical isolate of Bacteroides fragilis in Scandinavia: genetic analysis of a unique insertion sequence
    T R Walsh
    Department of Pathology and Microbiology, University of Bristol, Bristol, UK
    Scand J Infect Dis 37:676-9. 2005
  8. ncbi request reprint The emergence and implications of metallo-beta-lactamases in Gram-negative bacteria
    T R Walsh
    Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol, UK
    Clin Microbiol Infect 11:2-9. 2005
  9. ncbi request reprint Evolution of an integron carrying blaVIM-2 in Eastern Europe: report from the SENTRY Antimicrobial Surveillance Program
    Timothy R Walsh
    Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, UK
    J Antimicrob Chemother 52:116-9. 2003
  10. ncbi request reprint Combinatorial genetic evolution of multiresistance
    Timothy R Walsh
    Department of Molecular and Cellular Medicine, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Opin Microbiol 9:476-82. 2006

Collaborators

Detail Information

Publications77

  1. pmc Emergence of the extended-spectrum beta-lactamase GES-1 in a Pseudomonas aeruginosa strain from Brazil: report from the SENTRY antimicrobial surveillance program
    Mariana Castanheira
    Antimicrob Agents Chemother 48:2344-5. 2004
  2. ncbi request reprint Emerging metallo-beta-lactamase-mediated resistances: a summary report from the worldwide SENTRY antimicrobial surveillance program
    Thomas R Fritsche
    Jones Microbiology Institute Laboratories, North Liberty, IA 52317, USA
    Clin Infect Dis 41:S276-8. 2005
    ....
  3. doi request reprint Clinically significant carbapenemases: an update
    Timothy R Walsh
    Department of Medical Microbiology, School of Medical Sciences, Cardiff University, Heath Park Hospital, Cardiff, UK
    Curr Opin Infect Dis 21:367-71. 2008
    ..To provide a brief synopsis of studies which have extended our understanding of carbapenem resistance in clinical isolates in terms of epidemiology, mechanism of action and genetic factors affecting their carriage and spread...
  4. pmc Evaluation of a new Etest for detecting metallo-beta-lactamases in routine clinical testing
    Timothy R Walsh
    University of Bristol, United Kingdom
    J Clin Microbiol 40:2755-9. 2002
    ..The Etest MBL strip appears to be an acceptable diagnostic reagent to detect metallo-beta-lactamase phenotypes in the clinical microbiology laboratory...
  5. pmc Nucleotide and amino acid sequences of the metallo-beta-lactamase, ImiS, from Aeromonas veronii bv. sobria
    T R Walsh
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Microbiology and Pathology, Medical School, University of Bristol, United Kingdom
    Antimicrob Agents Chemother 42:436-9. 1998
    ..Both possessed the putative active-site sequence Asn-Tyr-His-Thr-Asp at positions 88 to 92, which is unique to the Aeromonas metallo-beta-lactamases...
  6. pmc Metallo-beta-lactamases: the quiet before the storm?
    Timothy R Walsh
    Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    Clin Microbiol Rev 18:306-25. 2005
    ..Their rapid dissemination is worrisome and necessitates the implementation of not just surveillance studies but also metallo-beta-lactamase inhibitor studies securing the longevity of important anti-infectives...
  7. ncbi request reprint Characterization of a carbapenemase-producing clinical isolate of Bacteroides fragilis in Scandinavia: genetic analysis of a unique insertion sequence
    T R Walsh
    Department of Pathology and Microbiology, University of Bristol, Bristol, UK
    Scand J Infect Dis 37:676-9. 2005
    ..The -10 promoter region was most similar to that of IS613 (100%) and the -35 promoter region to IS612 (100%), demonstrating the plasticity of these genetic regions...
  8. ncbi request reprint The emergence and implications of metallo-beta-lactamases in Gram-negative bacteria
    T R Walsh
    Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol, UK
    Clin Microbiol Infect 11:2-9. 2005
    ..There is also a pressing need for new, and hopefully novel, compounds active against pan-resistant Gram-negative bacteria--a growing problem that needs to be addressed by both government and industry...
  9. ncbi request reprint Evolution of an integron carrying blaVIM-2 in Eastern Europe: report from the SENTRY Antimicrobial Surveillance Program
    Timothy R Walsh
    Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, UK
    J Antimicrob Chemother 52:116-9. 2003
    ..This appears to be the first report of a metallo-beta-lactamase gene arising from a pathogenic strain in Eastern Europe...
  10. ncbi request reprint Combinatorial genetic evolution of multiresistance
    Timothy R Walsh
    Department of Molecular and Cellular Medicine, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Opin Microbiol 9:476-82. 2006
    ....
  11. ncbi request reprint The prevalence and mechanisms of vancomycin resistance in Staphylococcus aureus
    Timothy R Walsh
    Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, United Kingdom
    Annu Rev Microbiol 56:657-75. 2002
    ..There may be many different ways in which strains achieve this endpoint. Current knowledge and theories are summarized...
  12. pmc Evaluation of current methods for detection of staphylococci with reduced susceptibility to glycopeptides
    T R Walsh
    Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Clin Microbiol 39:2439-44. 2001
    ..0 McF inoculum, six different media were assessed against a selection of SRSV (n = 48) and MRSA (n = 12). Brain heart infusion agar yielded the highest sensitivity and specificity values: 88 and 88%, respectively...
  13. ncbi request reprint Characterization, cloning and sequence analysis of the inducible Ochrobactrum anthropi AmpC beta-lactamase
    C S Higgins
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    J Antimicrob Chemother 47:745-54. 2001
    ..Genomic searches of other non-gamma-subdivision bacteria revealed a homologous ampR-ampC cluster in the plant symbiont, Sinorhizobium meliloti...
  14. ncbi request reprint Aeromonas hydrophila AmpH and CepH beta-lactamases: derepressed expression in mutants of Escherichia coli lacking creB
    M B Avison
    Bristol Centre for Antimicrobial Research and Evaluation BCARE, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    J Antimicrob Chemother 46:695-702. 2000
    ..The entire cepH-containing fragment was sequenced, but it contained no genes that were obviously related to any known class of DNA-binding protein...
  15. ncbi request reprint beta-lactamase expression in Plesiomonas shigelloides
    M B Avison
    Bristol Centre for Antimicrobial Research and Evaluation BCARE, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    J Antimicrob Chemother 45:877-80. 2000
    ..3. The environmental isolates produced a variety of penicillinases, indicating that there is a reservoir of heterogeneous beta-lactamase genes in this species...
  16. ncbi request reprint The activity of vancomycin against heterogeneous vancomycin-intermediate methicillin-resistant Staphylococcus aureus explored using an in vitro pharmacokinetic model
    J Turner
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS1 5NB, UK
    J Antimicrob Chemother 48:727-30. 2001
    ..Short-term exposure of hVISA to vancomycin at gradient concentrations did not increase the proportion of cells with vancomycin-intermediate phenotype...
  17. ncbi request reprint A modified population analysis profile (PAP) method to detect hetero-resistance to vancomycin in Staphylococcus aureus in a UK hospital
    M Wootton
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Microbiology, North Bristol Health Trust and University of Bristol, Southmead Hospital, Westbury on Trym, Bristol, BS10 5NB, UK
    J Antimicrob Chemother 47:399-403. 2001
    ..The modified population analysis reliably detected vancomycin hetero-resistance in Mu 3 and identified no hVRSAs within the 100 MRSA sample...
  18. pmc Vancomycin susceptibility within methicillin-resistant Staphylococcus aureus lineages
    Robin A Howe
    Southmead Hospital, Bristol, United Kingdom
    Emerg Infect Dis 10:855-7. 2004
    ..Increasing antimicrobial resistance in genetically distinct pandemic clones may lead to MRSA infections that will become increasingly difficult to treat...
  19. pmc Plasmid location and molecular heterogeneity of the L1 and L2 beta-lactamase genes of Stenotrophomonas maltophilia
    M B Avison
    Bristol Centre for Antimicrobial Research and Evaluation BCARE, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 ITD, United Kingdom
    Antimicrob Agents Chemother 45:413-9. 2001
    ..It is therefore apparent that the L1 and L2 genes have evolved relatively quickly, perhaps because of their presence on a plasmid...
  20. ncbi request reprint Cefuroxime resistance in non-beta-lactamase Haemophilus influenzae is linked to mutations in ftsI
    K Straker
    Bristol Centre of Antimicrobial Research and Evaluation BCARE, Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, UK
    J Antimicrob Chemother 51:523-30. 2003
    ..Whilst other groups have examined the contribution of H. influenzae PBPs in ampicillin resistance, this is the first report analysing their role in CXM(R)...
  21. ncbi request reprint Role of the 'cre/blr-tag' DNA sequence in regulation of gene expression by the Aeromonas hydrophila beta-lactamase regulator, BlrA
    Matthew B Avison
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    J Antimicrob Chemother 53:197-202. 2004
    ....
  22. pmc Complete Sequence of p07-406, a 24,179-base-pair plasmid harboring the blaVIM-7 metallo-beta-lactamase gene in a Pseudomonas aeruginosa isolate from the United States
    Hongyang Li
    Department of Cellular Molecular Medicine, University of Bristol, Bristol BS8 1TD, United Kingdom
    Antimicrob Agents Chemother 52:3099-105. 2008
    ..This is the first plasmid to be sequenced carrying an MBL gene and highlights the amelioration of DNA segments from disparate origins, most noticeably from plant pathogens...
  23. ncbi request reprint Resistance to antibiotics and biocides among non-fermenting Gram-negative bacteria
    C S Higgins
    Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, University Walf, Bristol BS8 1TD, UK
    Clin Microbiol Infect 7:308-15. 2001
    ..To investigate the antibiotic and biocide susceptibilities of clinical isolates of rarely encountered Gram-negative, non-fermenting bacteria...
  24. ncbi request reprint A TEM-2beta-lactamase encoded on an active Tn1-like transposon in the genome of a clinical isolate of Stenotrophomonas maltophilia
    M B Avison
    Bristol Centre for Antimicrobial Research and Evaluation BCARE, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    J Antimicrob Chemother 46:879-84. 2000
    ..This represents the first identification of a TEM beta-lactamase in S. maltophilia and the first evidence that this important clinical pathogen is able to act as a reservoir for mobile beta-lactamase genes in the hospital environment...
  25. ncbi request reprint Analysis of AmpC beta-lactamase expression and sequence in biochemically atypical ceftazidime-resistant Enterobacteriaceae from paediatric patients
    Matthew B Avison
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology and Microbiology, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    J Antimicrob Chemother 53:584-91. 2004
    ..To analyse the variation of ampC beta-lactamase gene sequence and expression in biochemically atypical Enterobacteriaceae isolates, and to identify them definitively...
  26. ncbi request reprint Common regions e.g. orf513 and antibiotic resistance: IS91-like elements evolving complex class 1 integrons
    Mark A Toleman
    Bristol Centre for Antimicrobial Research and Evaluation BCARE, Department of Cellular and Molecular Medicine, School of Medical Sciences, University of Bristol, University Walk Bristol BS8 1TD, UK
    J Antimicrob Chemother 58:1-6. 2006
    ..In this article, we provide a brief description of these new and clinically important mobile elements, and how they are able to mobilize antibiotic resistance genes...
  27. ncbi request reprint Expression of tcaA and mprF and glycopeptide resistance in clinical glycopeptide-intermediate Staphylococcus aureus (GISA) and heteroGISA strains
    M Wootton
    Department of Pathology and Microbiology, Bristol Centre for Antimicrobial Research and Evaluation, Medical Sciences, University of Bristol, and North Bristol Healthcare Trust, Southmead Hospital, Bristol, UK
    Biochim Biophys Acta 1726:326-7. 2005
    ..This study reveals that expression levels of both genes are similar in groups of clinical GISA, heteroGISA and glycopeptide susceptible strains, suggesting no association with clinical isolates...
  28. ncbi request reprint A novel metallo-beta-lactamase, Mbl1b, produced by the environmental bacterium Caulobacter crescentus
    A M Simm
    Department of Pathology and Microbiology, University of Bristol, University Walk, UK
    FEBS Lett 509:350-4. 2001
    ..The main differences between Mbl1 and L1 are in the N-terminal region...
  29. ncbi request reprint pUb6060: a broad-host-range, DNA polymerase-I-independent ColE2-like plasmid
    M B Avison
    Department of Pathology and Microbiology, Bristol Centre for Antimicrobial Research and Evaluation, Bristol, BS8 1TD, United Kingdom
    Plasmid 45:88-100. 2001
    ..Additionally, it carries two ORFs encoding products of unknown function. The pUB6060 replicon maintains a moderate plasmid copy number (10 per chromosome copy) and permits replication in diverse gram-negative bacteria...
  30. ncbi request reprint Escherichia coli CreBC is a global regulator of gene expression that responds to growth in minimal media
    M B Avison
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, United Kingdom
    J Biol Chem 276:26955-61. 2001
    ..The diverse functions encoded by the cre regulon suggest that CreBC is a global regulator that sits right at the heart of metabolic control in Escherichia coli...
  31. ncbi request reprint Novel mechanism of hydrolysis of therapeutic beta-lactams by Stenotrophomonas maltophilia L1 metallo-beta-lactamase
    J Spencer
    Department of Pathology and Microbiology, University of Bristol School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 276:33638-44. 2001
    ....
  32. doi request reprint Induction of beta-lactamase production in Aeromonas hydrophila is responsive to beta-lactam-mediated changes in peptidoglycan composition
    Amy E Tayler
    Department of Cellular and Molecular Medicine, University of Bristol, Bristol BS8 1TD, UK
    Microbiology 156:2327-35. 2010
    ..Taken together, these data strongly suggest that the Aeromonas spp. beta-lactamase regulatory sensor kinase, BlrB, responds to the concentration of monomer-disaccharide-pentapeptide in peptidoglycan...
  33. pmc Bulgecin A: a novel inhibitor of binuclear metallo-beta-lactamases
    Alan M Simm
    Department of Pathology and Microbiology, University of Bristol, University Walk, Bristol BS8 1TD, UK
    Biochem J 387:585-90. 2005
    ..Docking experiments support the conclusion that bulgecin A co-ordinates to the zinc II site in metallo-beta-lactamases via the terminal sulphonate group on the sugar moiety...
  34. pmc ISCR elements: novel gene-capturing systems of the 21st century?
    Mark A Toleman
    Department of Cellular and Molecular Medicine, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Microbiol Mol Biol Rev 70:296-316. 2006
    ..It appears that ISCR elements have now firmly established themselves within that regimen...
  35. ncbi request reprint Italian metallo-beta-lactamases: a national problem? Report from the SENTRY Antimicrobial Surveillance Programme
    Mark A Toleman
    Department of Pathology and Microbiology, University of Bristol, Bristol, UK The JONES Group JMI Laboratories, North Liberty, IA, USA
    J Antimicrob Chemother 55:61-70. 2005
    ....
  36. ncbi request reprint A new approach to the inhibition of metallo-beta-lactamases
    James Spencer
    Department of Cellular and Molecular Medicine, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    Angew Chem Int Ed Engl 45:1022-6. 2006
  37. pmc A multicenter study evaluating the current strategies for isolating Staphylococcus aureus strains with reduced susceptibility to glycopeptides
    Mandy Wootton
    NPHS Microbiology Cardiff, University Hospital Wales, Cardiff, United Kingdom
    J Clin Microbiol 45:329-32. 2007
    ..MET exhibited the least interlaboratory variation. It is essential that laboratories use appropriate methods to detect GISA/hGISA strains so that the prevalence and clinical importance of these strains can be assessed properly...
  38. ncbi request reprint Crystal structure of Pseudomonas aeruginosa SPM-1 provides insights into variable zinc affinity of metallo-beta-lactamases
    Tanya A Murphy
    Department of Cellular and Molecular Medicine, University of Bristol School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    J Mol Biol 357:890-903. 2006
    ..We further predict that SPM-1 possesses the capacity to evolve variants of enhanced catalytic activity by point mutations altering geometry and hydrogen bonding in the vicinity of the second Zn2+ site...
  39. ncbi request reprint Reduced expression of the atl autolysin gene and susceptibility to autolysis in clinical heterogeneous glycopeptide-intermediate Staphylococcus aureus (hGISA) and GISA strains
    Mandy Wootton
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Cellular and Molecular Medicine, Medical Sciences, University of Bristol, Bristol BS1 8TD, UK
    J Antimicrob Chemother 56:944-7. 2005
    ..To assess a link between resistance to Triton X-100 induced autolysis (TIA) and lowered atl expression in a collection of clinical glycopeptide-intermediate Staphylococcus aureus (GISA) and heterogeneous GISA (hGISA)...
  40. ncbi request reprint Molecular characterization of SPM-1, a novel metallo-beta-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme
    Mark A Toleman
    Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, UK
    J Antimicrob Chemother 50:673-9. 2002
    ..The predicted molecular weight of the protein was 27 515 Da, significantly higher than that of IMP (25 041 Da) or VIM (25 322 Da). SPM-1 possesses a unique loop of 23 residues that accounts for the higher molecular mass...
  41. pmc blaVIM-7, an evolutionarily distinct metallo-beta-lactamase gene in a Pseudomonas aeruginosa isolate from the United States
    Mark A Toleman
    Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, United Kingdom
    Antimicrob Agents Chemother 48:329-32. 2004
    ..This is the first report of a mobile metallo-beta-lactamase gene, bla(VIM-7), being detected within the United States...
  42. ncbi request reprint Characterization of monomeric L1 metallo-beta -lactamase and the role of the N-terminal extension in negative cooperativity and antibiotic hydrolysis
    Alan M Simm
    Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 277:24744-52. 2002
    ..These data indicate that while the N terminus of L1 is not essential for beta-lactam hydrolysis, it is clearly important to its activity and substrate specificity...
  43. ncbi request reprint Genetic characterization of a novel metallo-beta-lactamase gene, blaIMP-13, harboured by a novel Tn5051-type transposon disseminating carbapenemase genes in Europe: report from the SENTRY worldwide antimicrobial surveillance programme
    Mark A Toleman
    Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, UK
    J Antimicrob Chemother 52:583-90. 2003
    ..The isolate was resistant to all antibiotics except amikacin, and displayed an imipenem MIC of 64 mg/L that decreased to 8 mg/L in the presence of EDTA. The resistance determinant was investigated...
  44. pmc Molecular and biochemical characterization of OXA-45, an extended-spectrum class 2d' beta-lactamase in Pseudomonas aeruginosa
    Mark A Toleman
    Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, United Kingdom
    Antimicrob Agents Chemother 47:2859-63. 2003
    ..Biochemically OXA-45 is most similar to OXA-18 in its substrate profile and inhibition by clavulanic acid and is a member of the 2d' class of beta-lactamases...
  45. pmc Activity of AZD2563, a novel oxazolidinone, against Staphylococcus aureus strains with reduced susceptibility to vancomycin or linezolid
    Robin A Howe
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, and Department of Pathology and Microbiology, School of Medical Sciences, Clifton, United Kingdom
    Antimicrob Agents Chemother 47:3651-2. 2003
    ..Cross-resistance with linezolid was seen in laboratory-generated mutants, and for these strains the MIC of AZD2563 was twofold higher than that of linezolid...
  46. pmc Biochemical characterization of the acquired metallo-beta-lactamase SPM-1 from Pseudomonas aeruginosa
    Tanya A Murphy
    Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, United Kingdom
    Antimicrob Agents Chemother 47:582-7. 2003
    ....
  47. pmc Comparative bactericidal activities of daptomycin and vancomycin against glycopeptide-intermediate Staphylococcus aureus (GISA) and heterogeneous GISA isolates
    Mandy Wootton
    Bristol Centre for Antimicrobial Research and Evaluation, Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, United Kingdom
    Antimicrob Agents Chemother 50:4195-7. 2006
    ..For all isolates, the daptomycin MIC50 and MIC90 are four times lower and the log drops in viable counts at 6 h and 24 h are significantly greater than those for vancomycin...
  48. ncbi request reprint Genetic linkage of the penicillinase gene, amp, and blrAB, encoding the regulator of beta-lactamase expression in Aeromonas spp
    Pannika Niumsup
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology and Microbiology, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    J Antimicrob Chemother 51:1351-8. 2003
    ..The same hierarchy is seen following beta-lactam challenge of A. hydrophila T429125, and correlates with the number of blr-tag sequences (TTCAC) found upstream of each beta-lactamase gene: ampH (one), cepH (two) and imiH (three)...
  49. pmc Pharmacodynamics of ceftazidime plus the serine beta-lactamase inhibitor AM-112 against Escherichia coli containing TEM-1 and CTX-M-1 beta-lactamases
    Karen E Bowker
    Bristol Centre for Antimicrobial Research and Evaluation, North Bristol NHS Trust, Department of Medical Microbiology, Southmead Hospital, Westbury on Trym, Bristol, BS10 5NB United Kingdom
    Antimicrob Agents Chemother 48:4482-4. 2004
    ..Ceftazidime alone was less effective than ceftazidime plus AM-112, and a single dose was more effective than three fractionated doses...
  50. ncbi request reprint Differential regulation of L1 and L2 beta-lactamase expression in Stenotrophomonas maltophilia
    Matthew B Avison
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    J Antimicrob Chemother 49:387-9. 2002
    ..The frequency of isolating type 3 mutants is c. 10(-9), however, implying that there is a significant overlap between the regulatory mechanisms...
  51. ncbi request reprint Preliminary analysis of the genetic basis for vancomycin resistance in Staphylococcus aureus strain Mu50
    Matthew B Avison
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    J Antimicrob Chemother 49:255-60. 2002
    ..These observations will inform targeted experiments aimed at a complete understanding of the mechanism(s) of vancomycin resistance in S. aureus Mu50 and other VRSA strains...
  52. pmc Thermodynamics of RTA3 peptide binding to membranes and consequences for antimicrobial activity
    Ayman Hawrani
    Department of Cellular and Molecular Medicine, Bristol University, Bristol, BS8 1TD, UK
    Biochim Biophys Acta 1798:1254-62. 2010
    ....
  53. doi request reprint Origin of low mammalian cell toxicity in a class of highly active antimicrobial amphipathic helical peptides
    Ayman Hawrani
    Department of Cellular and Molecular Medicine, Bristol University, Bristol BS8 1TD, United Kingdom
    J Biol Chem 283:18636-45. 2008
    ..We highlight a significant role for tryptophan in promoting binding of amphipathic helical peptides to neutral bilayers, augmenting the arsenal of strategies to reduce mammalian toxicity in antimicrobial peptides...
  54. pmc Effects of phenotype and genotype on methods for detection of extended-spectrum-beta-lactamase-producing clinical isolates of Escherichia coli and Klebsiella pneumoniae in Norway
    Ståle Tofteland
    Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway
    J Clin Microbiol 45:199-205. 2007
    ..coli strains. The relatively high proportion of ESBL-producing E. coli organisms with a low ceftazidime MIC in Norway emphasizes that cefpodoxime alone or both cefotaxime and ceftazidime should be used as substrates for ESBL detection...
  55. pmc Integrons containing the VIM-2 metallo-beta-lactamase gene among imipenem-resistant Pseudomonas aeruginosa strains from different Chinese hospitals
    Yun Song Yu
    Infectious Disease Department, The 1st Affiliated Hospital, Medical School, Zhejiang University, The Key Laboratory of the Infectious Diseases of Public Health Ministry, Hangzhou, Zhejiang, China
    J Clin Microbiol 44:4242-5. 2006
    ..Fourteen isolates were confirmed to contain the VIM-2 metallo-beta-lactamase gene. Twelve isolates harbored two kinds of class 1 integron, containing both VIM-2- and aminoglycoside-resistant genes...
  56. ncbi request reprint Nosocomial outbreak of CTX-M-15-producing E. coli in Norway
    Umaer Naseer
    Reference Centre for Detection of Antimicrobial Resistance K res, Department of Microbiology and Infection Control, University Hospital of Northern Norway, Norway
    APMIS 115:120-6. 2007
    ..The outbreak emphasises the epidemic potential of multiple-antibiotic-resistant CTX-M-15-producing E. coli also in a country with low antibiotic usage and low prevalence of antimicrobial resistance...
  57. pmc blaVIM-2-harboring integrons isolated in India, Russia, and the United States arise from an ancestral class 1 integron predating the formation of the 3' conserved sequence
    Mark A Toleman
    Department of Medical Microbiology, University of Cardiff, Heath Park, Cardiff CF14 4XN, United Kingdom
    Antimicrob Agents Chemother 51:2636-8. 2007
    ..These unusual structures are likely to have arisen from an ancestral integron predating the formation of the 3' conserved sequence...
  58. ncbi request reprint hGISA: seek and ye shall find
    Robin A Howe
    Lancet 364:500-1. 2004
  59. pmc Global emergence of trimethoprim/sulfamethoxazole resistance in Stenotrophomonas maltophilia mediated by acquisition of sul genes
    Mark A Toleman
    School of Medicine at Cardiff University, Cardiff, Wales, U K
    Emerg Infect Dis 13:559-65. 2007
    ..maltophilia and are geographically widespread, findings that reinforce the need for ongoing resistance surveillance...
  60. pmc A multi-center blinded study on the efficiency of phenotypic screening methods to detect glycopeptide intermediately susceptible Staphylococcus aureus (GISA) and heterogeneous GISA (h-GISA)
    Andreas Voss
    Radboud University Nijmegen Medical Centre, Nijmegen University Centre of Infectious Diseases, The Netherlands
    Ann Clin Microbiol Antimicrob 6:9. 2007
    ..To determine the true incidence of hGISA/GISA and its consequent clinical impact, methods must be defined that will reliably and reproducibly discriminate these resistant phenotypes from vancomycin susceptible S. aureus (VSSA)...
  61. pmc Dissemination of CTX-M-15 beta-lactamase genes carried on Inc FI and FII plasmids among clinical isolates of Escherichia coli in a university hospital in Istanbul, Turkey
    Nevriye Gonullu
    Department of Microbiology and Clinical Microbiology, Cerrahpasa Faculty of Medicine, University of Istanbul, Istanbul 34303, Turkey
    J Clin Microbiol 46:1110-2. 2008
    ..Plasmid typing of transconjugates carrying bla(CTX-M-15) showed that most isolates belonged to the Inc/rep FII group but that one isolate also belonged to the FI group...
  62. doi request reprint The AmpC phenotype in Norwegian clinical isolates of Escherichia coli is associated with an acquired ISEcp1-like ampC element or hyperproduction of the endogenous AmpC
    Bjørg Haldorsen
    Reference Centre for Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway
    J Antimicrob Chemother 62:694-702. 2008
    ..The aim of the study was to examine resistance mechanisms associated with an AmpC phenotype in Norwegian clinical isolates of Escherichia coli...
  63. ncbi request reprint Over-expression, purification, and characterization of metallo-beta-lactamase ImiS from Aeromonas veronii bv. sobria
    Patrick A Crawford
    Department of Chemistry and Biochemistry, 112 Hughes Hall, Miami University, Oxford, OH 45056, USA
    Protein Expr Purif 36:272-9. 2004
    ..This over-expression protocol now allows for detailed spectroscopic and mechanistic studies on ImiS as well as site-directed mutants of ImiS to be prepared for future structure/function studies...
  64. ncbi request reprint Characterization of fluoroquinolone-resistant beta-hemolytic Streptococcus spp. isolated in North America and Europe including the first report of fluoroquinolone-resistant Streptococcus dysgalactiae subspecies equisimilis: report from the SENTRY Antimicr
    Douglas J Biedenbach
    JMI Laboratories, North Liberty, IA 52317, USA
    Diagn Microbiol Infect Dis 55:119-27. 2006
    ....
  65. pmc Molecular characterization of a beta-lactamase gene, blaGIM-1, encoding a new subclass of metallo-beta-lactamase
    Mariana Castanheira
    Disciplina de Doencas Infecciosas e Parasitarias, Universidade Federal de Sao Paulo, Brazil
    Antimicrob Agents Chemother 48:4654-61. 2004
    ..GIM-1 appears to be a unique MbetaL, which is located in a distinct integron structure, and represents the fourth subclass of mobile MbetaL enzymes to be characterized...
  66. pmc Integron carrying a novel metallo-beta-lactamase gene, blaIMP-16, and a fused form of aminoglycoside-resistant gene aac(6')-30/aac(6')-Ib': report from the SENTRY Antimicrobial Surveillance Program
    Rodrigo E Mendes
    Disciplina de Doencas Infecciosas e Parasitarias, Universidade Federal de Sao Paulo, Sao Paulo, Brazil
    Antimicrob Agents Chemother 48:4693-702. 2004
    ..36 microM(-1) . s(-1)). This report further emphasizes the spread of metallo-beta-lactamase genes and their close association with various aminoglycoside resistance genes...
  67. pmc First isolation of bla(VIM-2) in Latin America: report from the SENTRY Antimicrobial Surveillance Program
    Rodrigo E Mendes
    Antimicrob Agents Chemother 48:1433-4. 2004
  68. ncbi request reprint Dissemination and diversity of metallo-beta-lactamases in Latin America: report from the SENTRY Antimicrobial Surveillance Program
    Helio S Sader
    The JONES Group JMI Laboratories, 345 Beaver Kreek Centre, Suite A, North Liberty, IA, USA
    Int J Antimicrob Agents 25:57-61. 2005
    ..The emergence and dissemination of mobile MbetaL-producing isolates represent an alarming factor for increasing resistance to carbapenems in several medical centres evaluated by the SENTRY Program in Latin America...
  69. pmc Characterization of an integron carrying blaIMP-1 and a new aminoglycoside resistance gene, aac(6')-31, and its dissemination among genetically unrelated clinical isolates in a Brazilian hospital
    Rodrigo E Mendes
    Laboratório Especial de Microbiologia Clínica and Laboratório ALERTA, Division of Infectious Disease, Federal University of Sao Paulo, Sao Paulo, Brazil
    Antimicrob Agents Chemother 51:2611-4. 2007
    ..This report describes the ability of nonfermentative nosocomial pathogens to acquire and disseminate antimicrobial resistance determinants...
  70. ncbi request reprint Emerging epidemic of metallo-beta-lactamase-mediated resistances
    Ronald N Jones
    Diagn Microbiol Infect Dis 51:77-84. 2005
  71. pmc Functional characterization of OXA-57, a class D beta-lactamase from Burkholderia pseudomallei
    Karen E Keith
    Department of Biological Sciences, CMMI, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom
    Antimicrob Agents Chemother 49:1639-41. 2005
    ..Comparative kinetic analyses of wild-type and mutant forms of B. pseudomallei OXA-57 are reported. Implications of these data for beta-lactam resistance and the proposed role of Ser-104 in beta-lactam hydrolysis are discussed...
  72. ncbi request reprint Pseudomonas aeruginosa strains harbouring an unusual blaVIM-4 gene cassette isolated from hospitalized children in Poland (1998-2001)
    Jan Patzer
    The Children s Memorial Health Institute, Warsaw, Poland
    J Antimicrob Chemother 53:451-6. 2004
    ..These strains were investigated further to determine the mechanism of resistance...
  73. pmc Antimicrobial susceptibility and epidemiology of a worldwide collection of Chryseobacterium spp: report from the SENTRY Antimicrobial Surveillance Program (1997-2001)
    Jeffrey T Kirby
    The JONES Group JMI Laboratories, North Liberty, Iowa, USA
    J Clin Microbiol 42:445-8. 2004
    ..0% susceptibility) followed by rifampin (MIC(90), 2 microg/ml and 85.7% susceptibility). Trimethoprim-sulfamethoxazole, ciprofloxacin, and piperacillin-tazobactam also showed reasonable activity; vancomycin showed poor potency...
  74. ncbi request reprint Analysis of Salmonella spp. with resistance to extended-spectrum cephalosporins and fluoroquinolones isolated in North America and Latin America: report from the SENTRY Antimicrobial Surveillance Program (1997-2004)
    Douglas J Biedenbach
    JMI Laboratories, 345 Beaver Kreek Centre, Suite A, North Liberty, IA 52317, USA
    Diagn Microbiol Infect Dis 54:13-21. 2006
    ..No QRDR trends over time were detected in North America, but increased resistance was observed in Latin America...
  75. ncbi request reprint Penicillin-derived inhibitors that simultaneously target both metallo- and serine-beta-lactamases
    John D Buynak
    Department of Chemistry, Southern Methodist University, Dallas, TX 75275 0314, USA
    Bioorg Med Chem Lett 14:1299-304. 2004
    ..Selected (mercaptomethyl)penicillinates are shown to inactivate both metallo- and serine-beta-lactamases and to display synergism with piperacillin against beta-lactamase producing strains...
  76. ncbi request reprint Salmonella bloodstream infections: report from the SENTRY Antimicrobial Surveillance Program (1997-2001)
    Jennifer M Stephen
    The JONES Group JMI Laboratories, 345 Beaver Kreek Centre, Suite A, North Liberty, IA 52317, USA
    Int J Antimicrob Agents 22:395-405. 2003
    ..BSIs for the commonly used antimicrobial classes. With the exception of S. Typhimurium DT104, most Salmonella spp. remain highly susceptible to the tested antimicrobials that maybe utilized for Salmonella BSI...
  77. ncbi request reprint Evolution and dissemination of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae: epidemiology and molecular report from the SENTRY Antimicrobial Surveillance Program (1997-2003)
    Joseph R DiPersio
    Summa Health System, Department of Pathology, Akron, OH, USA
    Diagn Microbiol Infect Dis 51:1-7. 2005
    ..pneumoniae clones that have the ability to cause sustained epidemic outbreaks in US medical centers...