M D Walkinshaw

Summary

Affiliation: University of Edinburgh
Country: UK

Publications

  1. ncbi Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA
    M D Walkinshaw
    Institute of Cell and Molecular Biology, The King s Buildings, University of Edinburgh, EH9 3JR, Edinburgh, United Kingdom
    Mol Cell 9:187-94. 2002
  2. ncbi Crystallization and preliminary X-ray analysis of ocr, the product of gene 0.3 of bacteriophage T7
    S S Sturrock
    Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, Scotland
    Acta Crystallogr D Biol Crystallogr 57:1652-4. 2001
  3. ncbi Purification of factor X by hydrophobic interaction chromatography
    H Husi
    Centre for Genome Research, The University of Edinburgh, UK
    J Chromatogr B Biomed Sci Appl 755:367-71. 2001
  4. ncbi Structural and spectroscopic analysis of the F393H mutant of flavocytochrome P450 BM3
    T W Ost
    Department of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JJ, UK
    Biochemistry 40:13430-8. 2001
  5. ncbi Two structures of cyclophilin 40: folding and fidelity in the TPR domains
    P Taylor
    Structural Biochemistry Group, Institute of Cell and Molecular Biology, The University of Edinburgh, Michael Swann Building, King s Buildings, Mayfield Road, EH9 3JR, Edinburgh, United Kingdom
    Structure 9:431-8. 2001
  6. ncbi Crystallization and preliminary X-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina
    S L Pealing
    Department of Chemistry, University of Edinburgh, Edinburgh, Scotland, United Kingdom
    J Struct Biol 127:76-8. 1999
  7. ncbi X-ray structures and analysis of 11 cyclosporin derivatives complexed with cyclophilin A
    J Kallen
    The University of Edinburgh, Michael Swann Building, Edinburgh, EH9 3JR, UK
    J Mol Biol 283:435-49. 1998
  8. ncbi X-ray structures of small ligand-FKBP complexes provide an estimate for hydrophobic interaction energies
    P Burkhard
    Department of Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, Basel, CH, 4056, Switzerland
    J Mol Biol 295:953-62. 2000
  9. ncbi Kinetic and crystallographic analysis of the key active site acid/base arginine in a soluble fumarate reductase
    C G Mowat
    Department of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK
    Biochemistry 40:12292-8. 2001
  10. ncbi A new conformation of the integrin-binding fragment of human VCAM-1 crystallizes in a highly hydrated packing arrangement
    P Taylor
    Structural Biochemistry Group, Institute of Cell and Molecular Biology, The University of Edinburgh, Michael Swann Building, King's Buildings, Edinburgh EH9 3JR, Scotland
    Acta Crystallogr D Biol Crystallogr 57:1579-83. 2001

Collaborators

Detail Information

Publications13

  1. ncbi Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA
    M D Walkinshaw
    Institute of Cell and Molecular Biology, The King s Buildings, University of Edinburgh, EH9 3JR, Edinburgh, United Kingdom
    Mol Cell 9:187-94. 2002
    ..This first structure of an antirestriction protein demonstrates the construction of structural mimetics of long segments of B-form DNA...
  2. ncbi Crystallization and preliminary X-ray analysis of ocr, the product of gene 0.3 of bacteriophage T7
    S S Sturrock
    Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, Scotland
    Acta Crystallogr D Biol Crystallogr 57:1652-4. 2001
    ..8 A. The presence of caesium was found to be required for good crystal growth. Anomalous X-ray data was used to identify possible positions for Se and Cs atoms in the unit cell...
  3. ncbi Purification of factor X by hydrophobic interaction chromatography
    H Husi
    Centre for Genome Research, The University of Edinburgh, UK
    J Chromatogr B Biomed Sci Appl 755:367-71. 2001
    ..This single purification step, in conjunction with classical purification strategies, is a powerful tool in generating high purity factor X and is based on resins which are readily available...
  4. ncbi Structural and spectroscopic analysis of the F393H mutant of flavocytochrome P450 BM3
    T W Ost
    Department of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JJ, UK
    Biochemistry 40:13430-8. 2001
    ....
  5. ncbi Two structures of cyclophilin 40: folding and fidelity in the TPR domains
    P Taylor
    Structural Biochemistry Group, Institute of Cell and Molecular Biology, The University of Edinburgh, Michael Swann Building, King s Buildings, Mayfield Road, EH9 3JR, Edinburgh, United Kingdom
    Structure 9:431-8. 2001
    ..The competitive binding of specific large immunophilins and other TPR-Hsp90 proteins provides a regulatory mechanism for Hsp90 chaperone activity...
  6. ncbi Crystallization and preliminary X-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina
    S L Pealing
    Department of Chemistry, University of Edinburgh, Edinburgh, Scotland, United Kingdom
    J Struct Biol 127:76-8. 1999
    ..359 A, b = 88.051 A, c = 77.473 A, and beta = 104.499 degrees. Anomalous data have also been collected from the NCIMB400 crystal allowing the heme iron positions to be identified...
  7. ncbi X-ray structures and analysis of 11 cyclosporin derivatives complexed with cyclophilin A
    J Kallen
    The University of Edinburgh, Michael Swann Building, Edinburgh, EH9 3JR, UK
    J Mol Biol 283:435-49. 1998
    ..The effect of small chemical differences on protein-ligand hydrogen-bonding, van der Waals interactions and water structure is presented...
  8. ncbi X-ray structures of small ligand-FKBP complexes provide an estimate for hydrophobic interaction energies
    P Burkhard
    Department of Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, Basel, CH, 4056, Switzerland
    J Mol Biol 295:953-62. 2000
    ..Analysis of the calculated buried surface areas of these related ligands provides an estimated van der Waals contribution to the binding energy of -0.5 kJ/A(2) for non-polar interactions between ligand and protein...
  9. ncbi Kinetic and crystallographic analysis of the key active site acid/base arginine in a soluble fumarate reductase
    C G Mowat
    Department of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK
    Biochemistry 40:12292-8. 2001
    ....
  10. ncbi A new conformation of the integrin-binding fragment of human VCAM-1 crystallizes in a highly hydrated packing arrangement
    P Taylor
    Structural Biochemistry Group, Institute of Cell and Molecular Biology, The University of Edinburgh, Michael Swann Building, King's Buildings, Edinburgh EH9 3JR, Scotland
    Acta Crystallogr D Biol Crystallogr 57:1579-83. 2001
    ..3 to 39.9 degrees. An analysis of the torsion angles shows that residues Ile88, Tyr89, Ser90, Pro92 and Glu96 play a major role in defining the interdomain conformations...
  11. ncbi X-ray structure of a monomeric cyclophilin A-cyclosporin A crystal complex at 2.1 A resolution
    V Mikol
    Preclinical Research, Sandoz AG, Basel, Switzerland
    J Mol Biol 234:1119-30. 1993
    ..The fact that this monomeric crystal form can be obtained shows that the formation of pentamer or decamer complexes is not a generally observed phenomenon and is not a prerequisite for biological activity...
  12. ncbi X-ray structure of a cyclophilin B/cyclosporin complex: comparison with cyclophilin A and delineation of its calcineurin-binding domain
    V Mikol
    Preclinical Research, Sandoz AG, Basel, Switzerland
    Proc Natl Acad Sci U S A 91:5183-6. 1994
    ..The three residues Arg90, Lys113, and Ala128 and the loop containing Arg158 on the surface of CypB are likely to modulate the differences in calcineurin inhibition between CypA and CypB...
  13. ncbi Structure of human cyclophilin and its binding site for cyclosporin A determined by X-ray crystallography and NMR spectroscopy
    J Kallen
    Preclinical Research, Sandoz Pharma AG, Basel, Switzerland
    Nature 353:276-9. 1991
    ..These results may help to provide a structural basis for rationalizing the immunosuppressive function of the cyclosporin-cyclophilin system and will also be important in the design of improved immunosuppressant drugs...