Tobias von der Haar

Summary

Affiliation: University of Kent
Country: UK

Publications

  1. pmc Optimized protein extraction for quantitative proteomics of yeasts
    Tobias von der Haar
    Protein Science Group, Department of Biosciences, University of Kent, Canterbury, United Kingdom
    PLoS ONE 2:e1078. 2007
  2. ncbi request reprint Regulated translational bypass of stop codons in yeast
    Tobias von der Haar
    Protein Science Group, Department of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
    Trends Microbiol 15:78-86. 2007
  3. ncbi request reprint Folding transitions during assembly of the eukaryotic mRNA cap-binding complex
    Tobias von der Haar
    Manchester Interdisciplinary Biocentre, c o Jackson s Mill, University of Manchester, P O Box 88, Manchester M60 1QD, UK
    J Mol Biol 356:982-92. 2006
  4. pmc A quantitative estimation of the global translational activity in logarithmically growing yeast cells
    Tobias von der Haar
    Protein Science Group, Department of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
    BMC Syst Biol 2:87. 2008
  5. pmc Probing the role of structural features of mouse PrP in yeast by expression as Sup35-PrP fusions
    Lyne Jossé
    Kent Fungal Group, School of Biosciences, University of Kent, Canterbury, UK
    Prion 6:201-10. 2012
  6. pmc Experimental and in silico modelling analyses of the gene expression pathway for recombinant antibody and by-product production in NS0 cell lines
    Emma J Mead
    School of Biosciences, University of Kent, Canterbury, United Kingdom
    PLoS ONE 7:e47422. 2012
  7. ncbi request reprint The mRNA cap-binding protein eIF4E in post-transcriptional gene expression
    Tobias von der Haar
    Post transcriptional Control Group, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, PO Box 88, Manchester M60 1QD, UK
    Nat Struct Mol Biol 11:503-11. 2004
  8. pmc Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast
    Gloria H Merritt
    Kent Fungal Group and Protein Science Group, School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
    Nucleic Acids Res 38:5479-92. 2010
  9. pmc The architecture of eukaryotic translation
    Dominique Chu
    School of Computing, University of Kent, CT2 7NF Canterbury, UK
    Nucleic Acids Res 40:10098-106. 2012
  10. doi request reprint Control and regulation of mRNA translation
    Emma J Mead
    Centre for Molecular Processing, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, U K
    Biochem Soc Trans 42:151-4. 2014

Collaborators

  • Dominique Chu
  • C Mark Smales
  • Mick Tuite
  • Gerhard Wagner
  • Alan G Hinnebusch
  • Katsura Asano
  • Bin Li
  • Emma J Mead
  • John E G McCarthy
  • Lyne Jossé
  • Lesley M Chiverton
  • Gloria H Merritt
  • Patrick Studte
  • Robert J C Gilbert
  • Hui He
  • John D Gross
  • Rosalyn J Masterton
  • Elaine B Martin
  • Jo Zenthon
  • Gary A Montague
  • Sarah K Spurgeon
  • Ricardo Marchante
  • Pierre Mugnier
  • Helen M Webb
  • Wesley R Naemi
  • Christian Bär
  • Sabrina Zink
  • Daniel Jablonowski
  • Raffael Schaffrath
  • Maria Nardelli
  • Steven Marsden
  • Andreas F P Sonnen
  • David I Stuart
  • Gregor Hofmann
  • Yulya Gordiyenko
  • Nathan J Moerke
  • Alexey A Lugovskoy
  • Alan B Sachs
  • Miki Ii
  • C Ranjit Singh

Detail Information

Publications22

  1. pmc Optimized protein extraction for quantitative proteomics of yeasts
    Tobias von der Haar
    Protein Science Group, Department of Biosciences, University of Kent, Canterbury, United Kingdom
    PLoS ONE 2:e1078. 2007
    ..Current protocols for the extraction of proteins from yeast cells are likely to introduce artifacts into quantification procedures because of incomplete or selective extraction...
  2. ncbi request reprint Regulated translational bypass of stop codons in yeast
    Tobias von der Haar
    Protein Science Group, Department of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
    Trends Microbiol 15:78-86. 2007
    ..Rather than being a translation 'error', stop-codon readthrough can have important effects on other cellular processes such as mRNA degradation and, in some cases, can confer a beneficial phenotype to the cell...
  3. ncbi request reprint Folding transitions during assembly of the eukaryotic mRNA cap-binding complex
    Tobias von der Haar
    Manchester Interdisciplinary Biocentre, c o Jackson s Mill, University of Manchester, P O Box 88, Manchester M60 1QD, UK
    J Mol Biol 356:982-92. 2006
    ..We present structural, kinetic and mutagenesis data that allow us to deduce some of the detailed folding transitions that take place during the eIF4E interactions...
  4. pmc A quantitative estimation of the global translational activity in logarithmically growing yeast cells
    Tobias von der Haar
    Protein Science Group, Department of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
    BMC Syst Biol 2:87. 2008
    ..Data on translational activity are difficult to generate experimentally under physiological conditions, however, translational activity as a parameter is in principle accessible through published genome-wide datasets...
  5. pmc Probing the role of structural features of mouse PrP in yeast by expression as Sup35-PrP fusions
    Lyne Jossé
    Kent Fungal Group, School of Biosciences, University of Kent, Canterbury, UK
    Prion 6:201-10. 2012
    ..Through this study we illustrate the benefits and limitations of yeast-based models for protein misfolding disorders...
  6. pmc Experimental and in silico modelling analyses of the gene expression pathway for recombinant antibody and by-product production in NS0 cell lines
    Emma J Mead
    School of Biosciences, University of Kent, Canterbury, United Kingdom
    PLoS ONE 7:e47422. 2012
    ..The models also highlight cell line specific limitations in the antibody expression pathway...
  7. ncbi request reprint The mRNA cap-binding protein eIF4E in post-transcriptional gene expression
    Tobias von der Haar
    Post transcriptional Control Group, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, PO Box 88, Manchester M60 1QD, UK
    Nat Struct Mol Biol 11:503-11. 2004
    ....
  8. pmc Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast
    Gloria H Merritt
    Kent Fungal Group and Protein Science Group, School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
    Nucleic Acids Res 38:5479-92. 2010
    ..We reassess current models of stop-codon recognition by eRF1 in the light of these new data...
  9. pmc The architecture of eukaryotic translation
    Dominique Chu
    School of Computing, University of Kent, CT2 7NF Canterbury, UK
    Nucleic Acids Res 40:10098-106. 2012
    ..Furthermore, we find that strongly ribosome limited regimes are optimal for cells in that they are resource efficient and simplify the dynamics of the system...
  10. doi request reprint Control and regulation of mRNA translation
    Emma J Mead
    Centre for Molecular Processing, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, U K
    Biochem Soc Trans 42:151-4. 2014
    ....
  11. doi request reprint A novel and versatile computational tool to model translation
    Dominique Chu
    School of Computing, University of Kent, Kent CT2 7NF, UK
    Bioinformatics 28:292-3. 2012
    ..The tool is written in Java, and is hence portable and can be parameterized for any organism...
  12. pmc The role of tRNA and ribosome competition in coupling the expression of different mRNAs in Saccharomyces cerevisiae
    Dominique Chu
    School of Computing, University of Kent, CT2 7NF, UK
    Nucleic Acids Res 39:6705-14. 2011
    ....
  13. ncbi request reprint Development of a novel yeast cell-based system for studying the aggregation of Alzheimer's disease-associated Abeta peptides in vivo
    Tobias von der Haar
    Protein Science Group, Department of Biosciences, University of Kent, Canterbury, UK
    Neurodegener Dis 4:136-47. 2007
    ..We conclude that we have established a useful new tool for studying the aggregation of Abeta peptides in an organism in vivo...
  14. doi request reprint Translation elongation can control translation initiation on eukaryotic mRNAs
    Dominique Chu
    School of Computing, University of Kent, Canterbury, UK
    EMBO J 33:21-34. 2014
    ..Codon usage thus evolved as a means to optimise translation on individual mRNAs, as well as global optimisation of ribosome availability. ..
  15. ncbi request reprint Studying the assembly of multicomponent protein and ribonucleoprotein complexes using surface plasmon resonance
    Tobias von der Haar
    Posttranscriptional Control Group, Department of Biomolecular Sciences, UMIST, PO Box 88, Manchester M60 1QD, UK
    Methods 29:167-74. 2003
    ..Ways to experimentally avoid or, where this is not possible, to control for these complications are discussed...
  16. ncbi request reprint Biophysical studies of the translation initiation pathway with immobilized mRNA analogs
    John E G McCarthy
    Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, United Kingdom
    Methods Enzymol 430:247-64. 2007
    ..In this chapter, we describe a number of approaches by which such immobilization can be achieved, followed by two specific examples for applications that use immobilized RNAs...
  17. doi request reprint Identification of the limitations on recombinant gene expression in CHO cell lines with varying luciferase production rates
    Emma J Mead
    Department of Biosciences, University of Kent, Canterbury, UK
    Biotechnol Bioeng 102:1593-602. 2009
    ..The implications for the development of strategies to engineer cells for enhanced recombinant protein production levels are discussed...
  18. doi request reprint tRNA and protein methylase complexes mediate zymocin toxicity in yeast
    Patrick Studte
    Institut fur Biologie, Bereich Genetik, Martin Luther Universitat, Halle Wittenberg, Weinbergweg 10, D 06120 Halle Saale, Germany
    Mol Microbiol 69:1266-77. 2008
    ..Sup35, we observe that SUP45 overexpression and sup45 mutants suppress zymocin. Intriguingly, this suppression correlates with upregulated levels of tRNA species targeted by zymocin's tRNase activity...
  19. ncbi request reprint Ribosome loading onto the mRNA cap is driven by conformational coupling between eIF4G and eIF4E
    John D Gross
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Cell 115:739-50. 2003
    ..Our data explain how mRNA, eIF4E, and eIF4G exists as a stable mRNP that may facilitate multiple rounds of ribosomal loading during translation initiation, a key determinant in the overall rate of protein synthesis...
  20. pmc The yeast eukaryotic initiation factor 4G (eIF4G) HEAT domain interacts with eIF1 and eIF5 and is involved in stringent AUG selection
    Hui He
    Division of Biology, Kansas State University, Manhattan, KS 66506, USA
    Mol Cell Biol 23:5431-45. 2003
    ..These results strongly suggest that the binding of the eIF4G HEAT domain to eIF1 and eIF5 is important for maintaining the integrity of the scanning ribosomal preinitiation complex...
  21. ncbi request reprint Intracellular translation initiation factor levels in Saccharomyces cerevisiae and their role in cap-complex function
    Tobias von der Haar
    Department of Biomolecular Sciences, Manchester, UK
    Mol Microbiol 46:531-44. 2002
    ..Overall, these data constitute an important basis for developing a quantitative model of the workings of the eukaryotic translation apparatus...
  22. pmc Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex
    Robert J C Gilbert
    Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, United Kingdom
    Proc Natl Acad Sci U S A 104:5788-93. 2007
    ..This is expected to increase the accessibility of the mRNA channel, thus enabling the 40S subunit to convert to a recruitment-competent state...