Thomas von Zglinicki

Summary

Affiliation: University of Newcastle
Country: UK

Publications

  1. ncbi request reprint Accumulation of single-strand breaks is the major cause of telomere shortening in human fibroblasts
    T von Zglinicki
    Institute of Pathology, Charite, Humboldt University Berlin, Germany
    Free Radic Biol Med 28:64-74. 2000
  2. ncbi request reprint Role of oxidative stress in telomere length regulation and replicative senescence
    T von Zglinicki
    Institute of Pathology, Charite, Humboldt University Berlin, Germany
    Ann N Y Acad Sci 908:99-110. 2000
  3. ncbi request reprint Short telomeres in patients with vascular dementia: an indicator of low antioxidative capacity and a possible risk factor?
    T von Zglinicki
    Institute of Pathology at the Evangelische Geriatriezentrum Berlin, Germany
    Lab Invest 80:1739-47. 2000
  4. doi request reprint Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress
    Shaheda Ahmed
    Crucible Laboratory, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, NE4 6BE, UK
    J Cell Sci 121:1046-53. 2008
  5. pmc Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response
    Diana Jurk
    Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne NE4 5PL, UK
    Aging Cell 11:996-1004. 2012
  6. pmc DNA damage in telomeres and mitochondria during cellular senescence: is there a connection?
    Joao F Passos
    Henry Wellcome Laboratory for Biogerontology Research, Institute for Ageing and Health, University of Newcastle, Newcastle upon Tyne NE4 6BE, UK
    Nucleic Acids Res 35:7505-13. 2007
  7. pmc Mitochondrial turnover in liver is fast in vivo and is accelerated by dietary restriction: application of a simple dynamic model
    Satomi Miwa
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Aging Cell 7:920-3. 2008
  8. pmc Feedback between p21 and reactive oxygen production is necessary for cell senescence
    Joao F Passos
    Ageing Research Laboratories, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Mol Syst Biol 6:347. 2010
  9. pmc Adult-onset, short-term dietary restriction reduces cell senescence in mice
    Chunfang Wang
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Aging (Albany NY) 2:555-66. 2010
  10. pmc A senescent cell bystander effect: senescence-induced senescence
    Glyn Nelson
    Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne, UK
    Aging Cell 11:345-9. 2012

Detail Information

Publications89

  1. ncbi request reprint Accumulation of single-strand breaks is the major cause of telomere shortening in human fibroblasts
    T von Zglinicki
    Institute of Pathology, Charite, Humboldt University Berlin, Germany
    Free Radic Biol Med 28:64-74. 2000
    ..Quantitatively, stress-mediated telomere damage contributes most to telomere shortening under standard conditions...
  2. ncbi request reprint Role of oxidative stress in telomere length regulation and replicative senescence
    T von Zglinicki
    Institute of Pathology, Charite, Humboldt University Berlin, Germany
    Ann N Y Acad Sci 908:99-110. 2000
    ..Free G-rich telomeric single strands are a strong inductor of the p53 pathway, and exposure of the overhangs seems to be the first step in the signal transduction cascade to replicative senescence...
  3. ncbi request reprint Short telomeres in patients with vascular dementia: an indicator of low antioxidative capacity and a possible risk factor?
    T von Zglinicki
    Institute of Pathology at the Evangelische Geriatriezentrum Berlin, Germany
    Lab Invest 80:1739-47. 2000
    ..No correlation was found to polymorphisms in the apolipoprotein E and glutathione-S-transferase genes. Telomere length may be an independent predictor for the risk of vascular dementia...
  4. doi request reprint Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress
    Shaheda Ahmed
    Crucible Laboratory, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, NE4 6BE, UK
    J Cell Sci 121:1046-53. 2008
    ..We propose protection of mitochondria under mild stress as a novel function of TERT...
  5. pmc Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response
    Diana Jurk
    Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne NE4 5PL, UK
    Aging Cell 11:996-1004. 2012
    ..Senescence-like neurons might be a source of oxidative and inflammatory stress and a contributor to brain aging...
  6. pmc DNA damage in telomeres and mitochondria during cellular senescence: is there a connection?
    Joao F Passos
    Henry Wellcome Laboratory for Biogerontology Research, Institute for Ageing and Health, University of Newcastle, Newcastle upon Tyne NE4 6BE, UK
    Nucleic Acids Res 35:7505-13. 2007
    ..Together, these data suggest a self-amplifying cycle between mitochondrial and telomeric DNA damage during cellular senescence...
  7. pmc Mitochondrial turnover in liver is fast in vivo and is accelerated by dietary restriction: application of a simple dynamic model
    Satomi Miwa
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Aging Cell 7:920-3. 2008
    ..83 days, and this decreased to 1.16 days following 3 months of dietary restriction, supporting the hypothesis that this intervention might promote mitochondrial turnover as a part of its beneficial effects...
  8. pmc Feedback between p21 and reactive oxygen production is necessary for cell senescence
    Joao F Passos
    Ageing Research Laboratories, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Mol Syst Biol 6:347. 2010
    ..These ROS in turn replenish short-lived DNA damage foci and maintain an ongoing DDR. We show that this loop is both necessary and sufficient for the stability of growth arrest during the establishment of the senescent phenotype...
  9. pmc Adult-onset, short-term dietary restriction reduces cell senescence in mice
    Chunfang Wang
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Aging (Albany NY) 2:555-66. 2010
    ....
  10. pmc A senescent cell bystander effect: senescence-induced senescence
    Glyn Nelson
    Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne, UK
    Aging Cell 11:345-9. 2012
    ..Hepatocytes bearing senescence markers clustered together in mice livers. Thus, senescent cells can induce a bystander effect, spreading senescence towards their neighbours in vitro and, possibly, in vivo...
  11. pmc Mitochondrial dysfunction and cell senescence--skin deep into mammalian aging
    Joao F Passos
    Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, NE4 5PL, UK
    Aging (Albany NY) 4:74-5. 2012
    ....
  12. ncbi request reprint Replicative senescence and the art of counting
    Thomas von Zglinicki
    Henry Wellcome Biogerontology Laboratory, Newcastle University, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE, UK
    Exp Gerontol 38:1259-64. 2003
    ..This identifies telomere-driven human cell replicative senescence as a stress response with high potential importance for human aging...
  13. ncbi request reprint Oxidative stress shortens telomeres
    Thomas von Zglinicki
    Dept Gerontology, University of Newcastle, Wolfson Research Centre, General Hospital, Newcastle upon Tyne, UK
    Trends Biochem Sci 27:339-44. 2002
    ..This might have evolved to block the growth of cells that have been exposed to a high risk of mutation...
  14. ncbi request reprint Telomeres and replicative senescence: Is it only length that counts?
    T von Zglinicki
    Department of Gerontology, University of Newcastle, Wolfson Research Centre, Newcastle General Hospital, Westgate Road, NE4 6BE, Newcastle upon Tyne, UK
    Cancer Lett 168:111-6. 2001
    ....
  15. pmc Mitochondrial dysfunction accounts for the stochastic heterogeneity in telomere-dependent senescence
    Joao F Passos
    Henry Wellcome Laboratory for Biogerontology Research, Institute for Ageing and Health, University of Newcastle, Newcastle upon Tyne, United Kingdom
    PLoS Biol 5:e110. 2007
    ..X foci. We propose that mitochondrial ROS is a major determinant of telomere-dependent senescence at the single-cell level that is responsible for cell-to-cell variation in replicative lifespan...
  16. ncbi request reprint Stochastic variation in telomere shortening rate causes heterogeneity of human fibroblast replicative life span
    Carmen Martin-Ruiz
    Henry Wellcome Biogerontology Laboratory, School of Clinical Medical Sciences, University of Newcastle, General Hospital, Newcastle NE4 6BE, United Kingdom
    J Biol Chem 279:17826-33. 2004
    ..The data show that heterogeneity of the human fibroblast replicative life span can be caused by significant stochastic cell-to-cell variation in telomere shortening...
  17. ncbi request reprint DNA damage response and cellular senescence in tissues of aging mice
    Chunfang Wang
    Ageing Research Laboratories, Institute for Ageing and Health and Center for Integrated Systems Biology of Ageing and Nutrition CISBAN, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
    Aging Cell 8:311-23. 2009
    ..X foci and telomeres even in crypts from very old mice, indicating that senescence in the crypt enterocytes is telomere independent. The results suggest that stress-dependent cell senescence could play a causal role for aging of mice...
  18. ncbi request reprint Mitochondria, telomeres and cell senescence
    Joao F Passos
    Henry Wellcome Laboratory for Biogerontology Research, Newcastle University, Newcastle NE4 6BE, UK
    Exp Gerontol 40:466-72. 2005
    ..Not much data exists concerning the role of mitochondria in this model. Here, we review evidence supporting an involvement of mitochondria in replicative senescence and a possible link to telomere shortening...
  19. doi request reprint Assessment of a large panel of candidate biomarkers of ageing in the Newcastle 85+ study
    Carmen Martin-Ruiz
    Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne NE4 5PL, UK
    Mech Ageing Dev 132:496-502. 2011
    ..As future data accrues on health outcomes within the cohort, it will become possible also to evaluate the predictive value of these and others of the candidate biomarkers...
  20. doi request reprint Frailty and the role of inflammation, immunosenescence and cellular ageing in the very old: cross-sectional findings from the Newcastle 85+ Study
    Joanna Collerton
    Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne NE4 5PL, United Kingdom
    Mech Ageing Dev 133:456-66. 2012
    ..Difficulties in operationalizing the Fried model, due to high levels of co-morbidity, limit its utility in the very old...
  21. ncbi request reprint Mitochondrial dysfunction and cell senescence: cause or consequence?
    Joao F Passos
    Henry Wellcome Laboratory for Biogerontology Research, Institute for Aging and Health, University of Newcastle, Newcastle upon Tyne, United Kingdom
    Rejuvenation Res 9:64-8. 2006
    ..Moreover, we suggest that this process might be more complex than originally formulated, because variations in nuclear gene expression involved in mitochondrion nucleus cross-talk are observed in both senescence and immortalization...
  22. doi request reprint The relationship between the aging- and photo-dependent T414G mitochondrial DNA mutation with cellular senescence and reactive oxygen species production in cultured skin fibroblasts
    Matthew J Birket
    Dermatological Sciences, Institute of Cellular Medicine, Medical School, Newcastle University, Newcastle upon Tyne, UK
    J Invest Dermatol 129:1361-6. 2009
    ..In conclusion, we propose that this particular mutation may have little effect on ROS production and the onset of cellular senescence in cultured fibroblasts...
  23. pmc Cellular senescence: unravelling complexity
    Joao F Passos
    Ageing Biology Laboratories and Centre for Integrated Systems Biology of Ageing and Nutrition CISBAN, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Age (Dordr) 31:353-63. 2009
    ..Finally, we illustrate the use of systems biology to address the mechanistic, functional and biochemical complexity of senescence...
  24. pmc Frailty and mortality are not influenced by mitochondrial DNA haplotypes in the very old
    Joanna Collerton
    Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne NE4 5PL, UK
    Neurobiol Aging 34:2889.e1-4. 2013
    ..Conflicting data from different populations underscore our conclusion that there is currently no compelling link between inherited mtDNA variants and aging. ..
  25. ncbi request reprint Tissue differences in BER-related incision activity and non-specific nuclease activity as measured by the comet assay
    Joanna P Gorniak
    Centre for Brain Ageing and Vitality, Human Nutrition Research Centre, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne NE4 5PL, UK
    Mutagenesis 28:673-81. 2013
    ..In conclusion, the comet-based DNA repair assay can be easily adapted to study a range of mammalian tissues. ..
  26. pmc A stochastic step model of replicative senescence explains ROS production rate in ageing cell populations
    Conor Lawless
    Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom
    PLoS ONE 7:e32117. 2012
    ..We conclude that longitudinal studies of single cells and their lineages are now required for testing hypotheses about roles and mechanisms of ROS increase during replicative senescence...
  27. doi request reprint An important role for CDK2 in G1 to S checkpoint activation and DNA damage response in human embryonic stem cells
    Irina Neganova
    Institute of Human Genetics, Newcastle University, International Centre for Life, Newcastle upon Tyne, United Kingdom
    Stem Cells 29:651-9. 2011
    ....
  28. ncbi request reprint Assessment of sleep and circadian rhythm disorders in the very old: the Newcastle 85+ Cohort Study
    Kirstie N Anderson
    Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne NE4 5PL, UK
    Age Ageing 43:57-63. 2014
    ..to examine the association between subjective and objective measures of sleep and wake and other health parameters in a cohort of the very old...
  29. pmc Capability and dependency in the Newcastle 85+ cohort study. Projections of future care needs
    Carol Jagger
    Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne NE4 5PL UK
    BMC Geriatr 11:21. 2011
    ..Little is known of the capabilities of the oldest old, the fastest growing age group in the population. We aimed to estimate capability and dependency in a cohort of 85 year olds and to project future demand for care...
  30. ncbi request reprint Stress defense in murine embryonic stem cells is superior to that of various differentiated murine cells
    Gabriele Saretzki
    Henry Wellcome Laboratory for Biogerontology, Newcastle General Hospital, University of Newcastle upon Tyne, Newcastle upon Tyne NE4 6BE, UK
    Stem Cells 22:962-71. 2004
    ....
  31. pmc Childhood growth, IQ and education as predictors of white blood cell telomere length at age 49-51 years: the Newcastle Thousand Families Study
    Mark S Pearce
    Institute of Health and Society, Newcastle University, Newcastle upon Tyne, England, United Kingdom
    PLoS ONE 7:e40116. 2012
    ..We investigated whether birth weight, infant growth, childhood cognition and adult height, as well as a range of lifestyle, socio-economic and educational factors, were associated with white blood cell telomere length at age 49-51 years...
  32. ncbi request reprint The role of telomeres in Etoposide induced tumor cell death
    Jessie Jeyapalan
    Henry Wellcome Biogerontology Laboratory, Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, UK
    Cell Cycle 3:1169-76. 2004
    ..However, upregulation of telomerase might be part of an attempted adaptative response, which protects cells by a mechanism that might be independent of telomere length maintenance...
  33. ncbi request reprint MitoQ counteracts telomere shortening and elongates lifespan of fibroblasts under mild oxidative stress
    Gabriele Saretzki
    Institute of Aging and Health, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
    Aging Cell 2:141-3. 2003
  34. ncbi request reprint TRF2 overexpression diminishes repair of telomeric single-strand breaks and accelerates telomere shortening in human fibroblasts
    Torsten Richter
    Henry Wellcome Biogerontology Laboratory and Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, University of Newcastle upon Tyne, UK
    Mech Ageing Dev 128:340-5. 2007
    ....
  35. ncbi request reprint Oxygen free radicals in cell senescence: are they signal transducers?
    Joao F Passos
    Henry Wellcome Laboratory for Biogerontology Research, Institute for Ageing and Health, Newcastle upon Tyne, UK
    Free Radic Res 40:1277-83. 2006
    ..ROS appear to be involved as inducers of DNA damage rather than as specific signalling molecules. The upregulation of ROS production often seen in premature senescence might be related to retrograde response initiated by mitochondria...
  36. doi request reprint Gross energy metabolism in mice under late onset, short term caloric restriction
    Kerry M Cameron
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne, UK
    Mech Ageing Dev 132:202-9. 2011
    ..Reduction of body mass and basal temperature were major compensatory mechanisms to reduced food availability during late-onset, short-term CR...
  37. ncbi request reprint A continuous correlation between oxidative stress and telomere shortening in fibroblasts
    Torsten Richter
    Henry Wellcome Laboratory for Biogerontology Research, Institute for Ageing and Health, University of Newcastle, Newcastle upon Tyne NE4 6BE, UK
    Exp Gerontol 42:1039-42. 2007
    ..This correlation suggests stress-mediated telomere DNA damage as an important determinant of telomere shortening...
  38. ncbi request reprint Telomere length predicts poststroke mortality, dementia, and cognitive decline
    Carmen Martin-Ruiz
    Institute for Ageing and Health, Newcastle General Hospital, Newcastle upon Tyne, United Kingdom
    Ann Neurol 60:174-80. 2006
    ..Early identification of those at risk is highly desirable to target interventions for secondary prevention. Telomere length in peripheral blood mononuclear cells was tested as prognostic risk marker...
  39. pmc Reactive oxygen species production and mitochondrial dysfunction in white blood cells are not valid biomarkers of ageing in the very old
    Laura Wiley
    Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne, United Kingdom
    PLoS ONE 9:e91005. 2014
    ..No associations were found between the measured parameters and age-related outcomes, including cognitive impairment, disability, co-morbidity and survival - questioning the validity of these parameters as candidate BoA in the very old. ..
  40. ncbi request reprint Replicative aging, telomeres, and oxidative stress
    Gabriele Saretzki
    Department of Gerontology, University of Newcastle, Newcastle upon Tyne NE6 4BE, United Kingdom
    Ann N Y Acad Sci 959:24-9. 2002
    ..These data suggest that parameters that characterise replicative senescence in vitro offer potential for understanding of, and intervention into, the aging process in vivo...
  41. ncbi request reprint Acquisition of aberrant DNA methylation is associated with frailty in the very old: findings from the Newcastle 85+ Study
    Joanna Collerton
    Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne, NE4 5PL, UK
    Biogerontology 15:317-28. 2014
    ..47 (95 % CI 0.26-0.85); n = 321, p = 0.013]. Overall this study suggests a potential role for age-related changes in CpG island methylation in the development of frailty. ..
  42. ncbi request reprint Immortalisation of human ovarian surface epithelium with telomerase and temperature-sensitive SV40 large T antigen
    Barry R Davies
    Department of Surgery, University of Newcastle, Newcastle upon Tyne, NE2 4HH, UK
    Exp Cell Res 288:390-402. 2003
    ..OSE-C2 cells may be useful in studying the physiology and differentiation of human OSE cells and provide insight into the poorly understood earliest stages of epithelial ovarian cancer...
  43. doi request reprint Cell sorting of young and senescent cells
    Graeme Hewitt
    Ageing Research Laboratories, Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle, UK
    Methods Mol Biol 1048:31-47. 2013
    ..Here, we describe various methods by which senescent cells can be sorted from mixed cultures and discuss how different methods impact on the posterior analysis of sorted populations. ..
  44. doi request reprint Measuring reactive oxygen species in senescent cells
    Joao F Passos
    Ageing Research Laboratories, Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Methods Mol Biol 965:253-63. 2013
    ..In this chapter, we present several protocols used for detection of (intra- and extracellular) ROS in live cells...
  45. pmc The Newcastle 85+ study: biological, clinical and psychosocial factors associated with healthy ageing: study protocol
    Joanna Collerton
    Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    BMC Geriatr 7:14. 2007
    ....
  46. ncbi request reprint Mitochondria and ageing: winning and losing in the numbers game
    Joao F Passos
    Centre for Integrated Systems Biology of Ageing and Nutrition, Henry Wellcome Laboratory for Biogerontology Research, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Bioessays 29:908-17. 2007
    ....
  47. ncbi request reprint DNA damage foci in mitosis are devoid of 53BP1
    Glyn Nelson
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Cell Cycle 8:3379-83. 2009
    ..This suggests a possible mechanism for cells to continue through the G(2) checkpoint with gammaH2A.X-flagged double strand breaks via inhibition of 53BP1-mediated DNA damage signalling...
  48. pmc Health and disease in 85 year olds: baseline findings from the Newcastle 85+ cohort study
    Joanna Collerton
    Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne NE4 5PL
    BMJ 339:b4904. 2009
    ....
  49. doi request reprint Monitoring DNA damage during cell senescence
    Glyn Nelson
    Ageing Research Laboratories, Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Methods Mol Biol 965:197-213. 2013
    ..Here, we describe various methods by which the DDR can be used as a robust marker of cellular senescence, and how to utilize a DDR marker to investigate the induction and stabilization of the senescent phenotype...
  50. doi request reprint Conserved cysteine residues in the mammalian lamin A tail are essential for cellular responses to ROS generation
    Vanja Pekovic
    School of Biological and Biomedical Sciences, Durham University, Durham, UK
    Aging Cell 10:1067-79. 2011
    ..Our findings suggest that the conserved C-terminal cysteine residues are essential for lamin A function and that loss or oxidative damage to these cysteine residues promotes cellular senescence...
  51. ncbi request reprint No association between socio-economic status and white blood cell telomere length
    Jean Adams
    Institute of Health and Society, Newcastle University, 21 Claremont Place, Newcastle upon Tyne NE2 4AA, UK
    Aging Cell 6:125-8. 2007
    ..The results of this, and the previous study, suggest that there is little evidence of a strong or consistent correlation between white blood cell telomere length and markers of socio-economic status...
  52. doi request reprint Measuring DNA repair incision activity of mouse tissue extracts towards singlet oxygen-induced DNA damage: a comet-based in vitro repair assay
    Sabine A S Langie
    Centre for Brain Ageing and Vitality, Human Nutrition Research Centre, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
    Mutagenesis 26:461-71. 2011
    ....
  53. ncbi request reprint Telomeres: influencing the rate of aging
    T von Zglinicki
    Institute of Pathology, Charite, Humboldt University, Berlin, Germany
    Ann N Y Acad Sci 854:318-27. 1998
    ..Not only the shortening of telomeres down to a "signal value," but accumulation of telomeric single-stranded DNA fragments, as well, could be relevant triggers for proliferative senescence...
  54. ncbi request reprint Human fibroblasts in vitro senesce with a donor-specific telomere length
    Violeta Serra
    Institute of Pathology, Charite, Humboldt University Berlin, Berlin, Germany
    FEBS Lett 516:71-4. 2002
    ..The data suggest a donor-specific, age-dependent regulation of the telomere length threshold that triggers senescence in human fibroblasts...
  55. ncbi request reprint Lysosomal redox-active iron is important for oxidative stress-induced DNA damage
    Tino Kurz
    School of Clinical Medical Sciences Gerontology, University of Newcastle upon Tyne, Newcastle General Hospital, Henry Wellcome Laboratory for Biogerontology Research, NE4 6BE, United Kingdom
    Ann N Y Acad Sci 1019:285-8. 2004
    ..Under oxidative stress conditions, this iron may then relocate to the nucleus and play an important role for DNA damage by taking part in Fenton reactions...
  56. ncbi request reprint Tumour-cell apoptosis after cisplatin treatment is not telomere dependent
    Jessie C Jeyapalan
    Henry Wellcome Biogerontology Laboratory, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom
    Int J Cancer 118:2727-34. 2006
    ..X at nontelomeric sites. Interstitial DNA damage appears to be more important than telomere loss or telomeric damage as inducer of the signal pathway towards apoptosis and/or growth arrest in cisplatin-treated tumour cells...
  57. ncbi request reprint Telomere shortening in human fibroblasts is not dependent on the size of the telomeric-3'-overhang
    Barbara Keys
    Henry Wellcome Biogerontology Laboratory, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
    Aging Cell 3:103-9. 2004
    ....
  58. ncbi request reprint Downregulation of multiple stress defense mechanisms during differentiation of human embryonic stem cells
    Gabriele Saretzki
    Crucible Lab, Institute of Human Genetics, Central Parkway, Newcastle upon Tyne NE1 3BZ, United Kingdom
    Stem Cells 26:455-64. 2008
    ..These results confirm earlier data obtained during mouse embryonic stem cell differentiation and are in accordance with evolutionary predictions...
  59. ncbi request reprint BJ fibroblasts display high antioxidant capacity and slow telomere shortening independent of hTERT transfection
    M Lorenz
    Institute of Pathology, Charite, Humboldt University, Berlin, Germany
    Free Radic Biol Med 31:824-31. 2001
    ..It is possible that the high antioxidative capacity of BJ cells, by minimizing the accumulation of genomic damage, is instrumental in the successful immortalization of these cells by telomerase...
  60. ncbi request reprint Stress, DNA damage and ageing -- an integrative approach
    T von Zglinicki
    Department of Gerontology, Institute for the Health of the Elderly, Wolfson Research Centre, University of Newcastle upon Tyne, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE, UK
    Exp Gerontol 36:1049-62. 2001
    ..We describe how an integrative approach to studying these mechanisms, coupled with computational modelling, may be of considerable importance in resolving some of the complexity of cellular ageing...
  61. ncbi request reprint Human cell senescence as a DNA damage response
    T von Zglinicki
    Henry Wellcome Biogerontology Laboratory, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
    Mech Ageing Dev 126:111-7. 2005
    ..Thus, cellular senescence can be regarded as a permanently maintained DNA damage response state. This suggests that antibodies against DNA damage foci components might be useful markers for senescent cells in vivo...
  62. pmc Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice
    Satomi Miwa
    Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne NE4 5PL, UK
    Nat Commun 5:3837. 2014
    ..We propose that lower abundance of free catalytic complex I components supports complex I assembly, efficacy of substrate utilization and minimal ROS production, enabling enhanced longevity. ..
  63. pmc Chronic inflammation induces telomere dysfunction and accelerates ageing in mice
    Diana Jurk
    Institute for Ageing and Health, Newcastle University, NE4 5PL, UK
    Nat Commun 2:4172. 2014
    ..These data indicate that systemic chronic inflammation can accelerate ageing via ROS-mediated exacerbation of telomere dysfunction and cell senescence in the absence of any other genetic or environmental factor. ..
  64. ncbi request reprint Telomere length is associated with left ventricular function in the oldest old: the Newcastle 85+ study
    Joanna Collerton
    Institute for Ageing and Health, Newcastle University, and Department of Cardiology, Freeman Hospital, Newcastle upon Tyne, UK
    Eur Heart J 28:172-6. 2007
    ..We measured telomere length in peripheral blood mononuclear cells and carried out echocardiography in a group of 85-year old subjects recruited from the community as part of the Newcastle 85+ Study...
  65. ncbi request reprint Mitochondrial dysfunction in osteoarthritis is associated with down-regulation of superoxide dismutase 2
    Christos Gavriilidis
    Newcastle University, Newcastle upon Tyne, UK
    Arthritis Rheum 65:378-87. 2013
    ..Superoxide dismutase 2 (SOD2) is down- regulated in osteoarthritis (OA). This study was undertaken to investigate the functional effects of this down-regulation in the context of oxidative damage and mitochondrial dysfunction...
  66. ncbi request reprint Telomere length in white blood cells is not associated with morbidity or mortality in the oldest old: a population-based study
    Carmen M Martin-Ruiz
    Henry Wellcome Biogerontology Research Laboratory, University of Newcastle, Newcastle upon Tyne, UK
    Aging Cell 4:287-90. 2005
    ..We conclude that blood monocyte telomere length is not a predictive indicator for age-related morbidity and mortality at ages over 85 years, possibly because of a high degree of telomere length instability in this group...
  67. doi request reprint Correction of radiolabel pulse-chase data by a mathematical model: application to mitochondrial turnover studies
    Satomi Miwa
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne NE4 5PL, UK
    Biochem Soc Trans 38:1322-8. 2010
    ..This example has important implications not only for similar pulse-chase experiments, but also in a more general context where comparable types of data are generated...
  68. ncbi request reprint Telomerase as a promising target for human cancer gene therapy
    Gabriele Saretzki
    Gerontology, Institute of Ageing and Health, Newcastle University, UK
    Drugs Today (Barc) 39:265-76. 2003
    ..Other important strategies are the use of telomerase promoters for the application of toxic or pro-apoptotic drugs into human cancer cells or the use of immunological properties of the telomerase enzyme for possible cancer therapies...
  69. doi request reprint Nutrition in advanced age: dietary assessment in the Newcastle 85+ study
    A J Adamson
    Human Nutrition Research Centre, Newcastle University, Newcastle upon Tyne, UK
    Eur J Clin Nutr 63:S6-18. 2009
    ..This paper describes a comparison of two different methods of dietary assessment within the Newcastle 85+ Study; a UK cohort study of health and ageing in the oldest old...
  70. ncbi request reprint Telomere shortening and haemodialysis
    Mark C Boxall
    Institute of Human Genetics and Institute for Ageing and Health, University of Newcastle upon Tyne, and Renal Unit, Newcastle upon Tyne Hospitals NHS Trust, Newcastle upon Tyne, UK
    Blood Purif 24:185-9. 2006
    ..Telomere shortening rate is increased by oxidative stress. In this study we have examined a possible relationship between oxidative stress and telomere shortening in haemodialysis...
  71. ncbi request reprint Telomeres as biomarkers for ageing and age-related diseases
    T von Zglinicki
    Henry Wellcome Laboratory for Biogerontology Research, Newcastle General Hospital, School of Clinical Medical Sciences Gerontology, University of Newcastle, Newcastle upon Tyne, NE4 6BE, UK
    Curr Mol Med 5:197-203. 2005
    ....
  72. ncbi request reprint hTERT gene dosage correlates with telomerase activity in human lung cancer cell lines
    Gabriele Saretzki
    Deptartment of Gerontology, University of Newcastle, Wolfson Research Centre, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE, UK
    Cancer Lett 176:81-91. 2002
    ..We found a significant correlation between hTERT gene dosage, hTERT mRNA expression and telomerase activity. Imbalances of chromosome 5p may exert functionally relevant hTERT gene dosage effects in human lung cancer...
  73. ncbi request reprint Proteasome inhibition by lipofuscin/ceroid during postmitotic aging of fibroblasts
    N Sitte
    Institute of Pathology, Clinics of Physical Medicine and Rehabilitation, and Institute of Medical Immunology, Charite, Humboldt University, Berlin, Germany
    FASEB J 14:1490-8. 2000
    ..Our results indicate that an accumulation of oxidized proteins (and lipids) such as lipofuscin/ceroid may actually cause further increases in damage accumulation during aging by inhibiting the proteasome...
  74. ncbi request reprint Similar gene expression pattern in senescent and hyperoxic-treated fibroblasts
    G Saretzki
    Institute of Pathology, Charite, Humboldt University Berlin, Germany
    J Gerontol A Biol Sci Med Sci 53:B438-42. 1998
    ..For all these cases, gene expression under hyperoxia was similar to that in senescent cells, suggesting that chronic mild hyperoxia is a valid model for accelerated senescence...
  75. ncbi request reprint Ribozyme cleavage of telomerase mRNA sensitizes breast epithelial cells to inhibitors of topoisomerase
    A Ludwig
    Institute of Pathology, , Humboldt-University, Berlin, Germany
    Cancer Res 61:3053-61. 2001
    ..The data validate a ribozyme approach for telomerase inhibition therapy in cancer and suggest that it might be combined advantageously with topoisomerase-directed chemotherapy...
  76. ncbi request reprint A DNA damage checkpoint response in telomere-initiated senescence
    Fabrizio d'Adda di Fagagna
    1 The Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, UK 2 Present address IFOM FIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milan, Italy
    Nature 426:194-8. 2003
    ..Thus, we propose that telomere-initiated senescence reflects a DNA damage checkpoint response that is activated with a direct contribution from dysfunctional telomeres...
  77. ncbi request reprint Extended lifespan and long telomeres in rectal fibroblasts from late-onset ulcerative colitis patients
    Katherine M Getliffe
    Department of Biological Sciences, University of Warwick, Coventry, UK
    Eur J Gastroenterol Hepatol 18:133-41. 2006
    ..The aim of this study is to investigate whether the impaired function of rectal fibroblasts in UC is due to accelerated telomere shortening, oxidative stress and premature senescence...
  78. ncbi request reprint Fat depot-specific characteristics are retained in strains derived from single human preadipocytes
    Tamara Tchkonia
    Department of Medicine, Boston University, Boston, MA 02118, USA
    Diabetes 55:2571-8. 2006
    ....
  79. ncbi request reprint Cdkn1a deletion improves stem cell function and lifespan of mice with dysfunctional telomeres without accelerating cancer formation
    Aaheli Roy Choudhury
    Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Carl Neuberg Str 1, 30625 Hannover, Germany
    Nat Genet 39:99-105. 2007
    ..This study provides experimental evidence that telomere dysfunction induces p21-dependent checkpoints in vivo that can limit longevity at the organismal level...
  80. pmc Relocalized redox-active lysosomal iron is an important mediator of oxidative-stress-induced DNA damage
    Tino Kurz
    Division of Pathology II, Medical Faculty, University of Linkoping, SE 581 85 Linkoping, Sweden
    Biochem J 378:1039-45. 2004
    ....
  81. doi request reprint Architectural changes in the thymus of aging mice
    Danielle Aw
    Host Response and Genes and Development Group, Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
    Aging Cell 7:158-67. 2008
    ..These changes within the thymic epithelial cells may be in part accountable for thymic atrophy and responsible for the decline in T-cell output...
  82. ncbi request reprint Premature senescence of mesothelial cells is associated with non-telomeric DNA damage
    Krzysztof Ksiazek
    Department of Pathophysiology, University of Medical Sciences, Swiecickiego 6, 60781 Poznan, Poland
    Biochem Biophys Res Commun 362:707-11. 2007
    ..These results may indicate that premature senescence of HPMCs is largely related to oxidative stress-induced DNA damage in non-telomeric regions of the genome...
  83. ncbi request reprint Telomere-driven replicative senescence is a stress response
    Thomas von Zglinicki
    Nat Biotechnol 21:229-30. 2003
  84. ncbi request reprint Extracellular superoxide dismutase is a major antioxidant in human fibroblasts and slows telomere shortening
    Violeta Serra
    Institute of Pathology and Research Laboratory Cardiology, Charite Hospital, D 10098 Berlin, Germany
    J Biol Chem 278:6824-30. 2003
    ....
  85. ncbi request reprint Mitochondrial dysfunction is a possible cause of accelerated senescence of mesothelial cells exposed to high glucose
    Krzysztof Ksiazek
    Department of Pathophysiology, University of Medical Sciences, Swiecickiego 6, 60781 Poznan, Poland
    Biochem Biophys Res Commun 366:793-9. 2008
    ..Together, these results indicate that high glucose may accelerate senescence of HPMCs by impairing mitochondrial function, resulting in overproduction of reactive oxygen species and extensive DNA damage...
  86. ncbi request reprint Methods for cell sorting of young and senescent cells
    Joao F Passos
    Henry Wellcome Laboratory for Biogerontology Research Newcastle University, Newcastle, UK
    Methods Mol Biol 371:33-44. 2007
    ..Here, we describe various methods by which cells that reach senescence early can be physically sorted out of a bulk of growing cells, and discuss how different methods can affect the posterior analysis of the sorted populations...
  87. pmc Nucleoplasmic LAP2alpha-lamin A complexes are required to maintain a proliferative state in human fibroblasts
    Vanja Pekovic
    School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, England, UK
    J Cell Biol 176:163-72. 2007
    ..Our results suggest that LAP2alpha and lamin A/C are involved in controlling Rb localization and phosphorylation, and a lack or mislocalization of either protein leads to cell cycle arrest in HDFs...
  88. ncbi request reprint Telomeres, telomerase and the cancer cell: an introduction
    Thomas von Zglinicki
    Cancer Lett 194:137-8. 2003
  89. pmc Science fact and the SENS agenda. What can we reasonably expect from ageing research?
    Huber Warner
    Buck Institute for Age Research, Novato, CA, USA
    EMBO Rep 6:1006-8. 2005