Ludovic Vallier


Affiliation: University of Cambridge
Country: UK


  1. Grandy R, Tomaz R, Vallier L. Modeling Disease with Human Inducible Pluripotent Stem Cells. Annu Rev Pathol. 2019;14:449-468 pubmed publisher
  2. Snijders K, Cooper J, Vallier L, Bertero A. Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9. Methods Mol Biol. 2019;1961:185-209 pubmed publisher
    ..When implemented in human pluripotent stem cells (hPSCs), the approach can be then efficiently applied to virtually any hPSC-derived human cell type at various stages of development or disease. ..
  3. Chia C, Madrigal P, Denil S, Martinez I, Garcia Bernardo J, El Khairi R, et al. GATA6 Cooperates with EOMES/SMAD2/3 to Deploy the Gene Regulatory Network Governing Human Definitive Endoderm and Pancreas Formation. Stem Cell Reports. 2019;12:57-70 pubmed publisher
    ..Taken together, our data position GATA6 as a gatekeeper to early human, but not murine, pancreatic ontogeny. ..
  4. Yiangou L, Grandy R, Morell C, Tomaz R, Osnato A, Kadiwala J, et al. Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics. Stem Cell Reports. 2019;12:165-179 pubmed publisher
    ..Thus, our synchronization method provides a robust approach to study cell cycle mechanisms in hPSCs. ..
  5. Serrano F, Bernard W, Granata A, Iyer D, Steventon B, Kim M, et al. A novel human pluripotent stem cell-derived neural crest model of Treacher Collins Syndrome shows defects in cell death and migration. Stem Cells Dev. 2018;: pubmed publisher
    ..In conclusion, the developed protocol permits the generation of the large number of NC cells required for developmental studies, disease modeling, and for drug discovery platforms in vitro. ..
  6. Segeritz C, Rashid S, de Brito M, Serra M, Ordonez A, Morell C, et al. hiPSC hepatocyte model demonstrates the role of unfolded protein response and inflammatory networks in α1-antitrypsin deficiency. J Hepatol. 2018;69:851-860 pubmed publisher
    ..This study also demonstrates the interest of using hepatic cells generated from human-induced pluripotent stem cells to model liver disease in vitro for uncovering new mechanisms with clinical relevance. ..
  7. Vallier L, Touboul T, Chng Z, Brimpari M, Hannan N, Millan E, et al. Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. PLoS ONE. 2009;4:e6082 pubmed publisher
  8. Pauklin S, Vallier L. Activin/Nodal signalling in stem cells. Development. 2015;142:607-19 pubmed publisher
    ..Moreover, we will discuss future directions and questions that currently remain unanswered on the role of Activin/Nodal signalling in stem cell self-renewal, differentiation and proliferation. ..
  9. Vallier L. Cell Cycle Rules Pluripotency. Cell Stem Cell. 2015;17:131-2 pubmed publisher
    ..Now in Cell, Gonzales et al. (2015) and colleagues demonstrate that factors controlling the G2/M phase are necessary to block pluripotency upon induction of differentiation. ..

More Information


  1. Yiangou L, Ross A, Goh K, Vallier L. Human Pluripotent Stem Cell-Derived Endoderm for Modeling Development and Clinical Applications. Cell Stem Cell. 2018;22:485-499 pubmed publisher
    ..Here we describe recent advances in methods for generating endodermal cell types from human pluripotent stem cells and their use for disease modeling and cell-based therapy. ..
  2. Soares F, Sheldon M, Rao M, Mummery C, Vallier L. International coordination of large-scale human induced pluripotent stem cell initiatives: Wellcome Trust and ISSCR workshops white paper. Stem Cell Reports. 2014;3:931-9 pubmed publisher
    ..The purpose was to discuss strategies for making thousands of hiPSC lines widely available with as few restrictions as possible while retaining financial viability and donor privacy. The outcome of these discussions is described here. ..
  3. Vallier L, Alexander M, Pedersen R. Conditional gene expression in human embryonic stem cells. Stem Cells. 2007;25:1490-7 pubmed
    ..Disclosure of potential conflicts of interest is found at the end of this article. ..
  4. El Khairi R, Vallier L. The role of hepatocyte nuclear factor 1? in disease and development. Diabetes Obes Metab. 2016;18 Suppl 1:23-32 pubmed publisher
    ..This review discusses the role of HNF1B in human and murine pancreas and liver development, summarizes the disease phenotypes and identifies areas for future investigations in HNF1B-associated diabetes and liver disease. ..
  5. Vallier L, Touboul T, Brown S, Cho C, Bilican B, Alexander M, et al. Signaling pathways controlling pluripotency and early cell fate decisions of human induced pluripotent stem cells. Stem Cells. 2009;27:2655-66 pubmed publisher
    ..Together these data reveal that human iPSCs rely on mechanisms similar to human ESCs to maintain their pluripotency and to control their differentiation, showing that these pluripotent cell types are functionally equivalent. ..
  6. Ortmann D, Vallier L. Variability of human pluripotent stem cell lines. Curr Opin Genet Dev. 2017;46:179-185 pubmed publisher
    ..Here, we summarise our knowledge concerning the origin of this variability and describe potential solutions currently available to bypass this major challenge. ..
  7. Vallier L, Reynolds D, Pedersen R. Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal default pathway. Dev Biol. 2004;275:403-21 pubmed
    ..The effects of Nodal on early differentiation illustrate how hESCs can augment mouse embryos as a model for analyzing mechanisms of early mammalian development. ..
  8. Bertero A, Madrigal P, Galli A, Hubner N, Moreno I, Burks D, et al. Activin/nodal signaling and NANOG orchestrate human embryonic stem cell fate decisions by controlling the H3K4me3 chromatin mark. Genes Dev. 2015;29:702-17 pubmed publisher
    ..Our results reveal the mechanisms by which extracellular factors coordinate chromatin status and cell fate decisions in hESCs. ..
  9. Vallier L, Mendjan S, Brown S, Chng Z, Teo A, Smithers L, et al. Activin/Nodal signalling maintains pluripotency by controlling Nanog expression. Development. 2009;136:1339-49 pubmed publisher
    ..This negative-feedback loop imposes stasis in neuroectoderm and mesendoderm differentiation, thereby maintaining the pluripotent status of human ESCs and mouse EpiSCs...
  10. Vallier L, Alexander M, Pedersen R. Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. J Cell Sci. 2005;118:4495-509 pubmed