Ludovic Vallier

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi Enhancing and diminishing gene function in human embryonic stem cells
    Ludovic Vallier
    Department of Surgery, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    Stem Cells 22:2-11. 2004
  2. ncbi Human embryonic stem cells: an in vitro model to study mechanisms controlling pluripotency in early mammalian development
    Ludovic Vallier
    Department of Surgery and Cambride Institute for Medical Research Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 2XY, UK
    Stem Cell Rev 1:119-30. 2005
  3. ncbi Inhibition of Activin/Nodal signaling promotes specification of human embryonic stem cells into neuroectoderm
    Joseph R Smith
    Department of Surgery and Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
    Dev Biol 313:107-17. 2008
  4. doi SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells
    Zhenzhi Chng
    Laboratory for Regenerative Medicine, West Forvie Building, Robinson Way, University of Cambridge, Cambridge, CB2 0SZ, UK
    Cell Stem Cell 6:59-70. 2010
  5. ncbi Heps with pep: direct reprogramming into human hepatocytes
    Ludovic Vallier
    Wellcome Trust Medical Research Council Stem Cell Institute, Anne McLaren Institute for Regenerative Medicine, Department of Surgery, West Forvie Site, Robinson Way, Cambridge CB20SZ, UK Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK Electronic address
    Cell Stem Cell 14:267-9. 2014
  6. pmc Inhibition of activin/nodal signalling is necessary for pancreatic differentiation of human pluripotent stem cells
    C H H Cho
    Wellcome Trust Medical Research Council Cambridge Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine, West Forvie Site, Robinson Way, Cambridge, UK
    Diabetologia 55:3284-95. 2012
  7. pmc Integration-deficient lentivectors: an effective strategy to purify and differentiate human embryonic stem cell-derived hepatic progenitors
    Guanghua Yang
    INSERM U 972, IFR 93, Bicetre Hospital, and Paul Brousse Hospital, Villejuif F 94807, France
    BMC Biol 11:86. 2013
  8. doi Signaling pathways controlling pluripotency and early cell fate decisions of human induced pluripotent stem cells
    Ludovic Vallier
    Laboratory for Regenerative Medicine, University of Cambridge, Cambridge CB2 0SZ, United Kingdom
    Stem Cells 27:2655-66. 2009
  9. pmc Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways
    Ludovic Vallier
    Department of Surgery and Laboratory For Regenerative Medicine, University of Cambridge, Cambridge, UK
    PLoS ONE 4:e6082. 2009
  10. pmc Activin/Nodal signalling maintains pluripotency by controlling Nanog expression
    Ludovic Vallier
    Department of Surgery and Laboratory For Regenerative Medicine, West Forvie Building, Robinson Way, University of Cambridge, Cambridge CB2 0SZ, UK
    Development 136:1339-49. 2009

Collaborators

Detail Information

Publications38

  1. ncbi Enhancing and diminishing gene function in human embryonic stem cells
    Ludovic Vallier
    Department of Surgery, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    Stem Cells 22:2-11. 2004
    ..Application of these gain- and loss-of-function approaches should have widespread use, not only in revealing the developmental roles of specific human genes, but also for their utility in modulating differentiation...
  2. ncbi Human embryonic stem cells: an in vitro model to study mechanisms controlling pluripotency in early mammalian development
    Ludovic Vallier
    Department of Surgery and Cambride Institute for Medical Research Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 2XY, UK
    Stem Cell Rev 1:119-30. 2005
    ..With the new perspective of these findings, we suggest a previously unrecognized role of TGF-beta pathway signaling in maintaining pluripotency at early stages of mammalian embryonic development...
  3. ncbi Inhibition of Activin/Nodal signaling promotes specification of human embryonic stem cells into neuroectoderm
    Joseph R Smith
    Department of Surgery and Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
    Dev Biol 313:107-17. 2008
    ..These results show that inhibition of Nodal signaling promotes neuronal specification, indicating a role for this pathway in controlling early neural development of pluripotent cells...
  4. doi SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells
    Zhenzhi Chng
    Laboratory for Regenerative Medicine, West Forvie Building, Robinson Way, University of Cambridge, Cambridge, CB2 0SZ, UK
    Cell Stem Cell 6:59-70. 2010
    ....
  5. ncbi Heps with pep: direct reprogramming into human hepatocytes
    Ludovic Vallier
    Wellcome Trust Medical Research Council Stem Cell Institute, Anne McLaren Institute for Regenerative Medicine, Department of Surgery, West Forvie Site, Robinson Way, Cambridge CB20SZ, UK Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK Electronic address
    Cell Stem Cell 14:267-9. 2014
    ..Now in Cell Stem Cell, two papers demonstrate efficient direct reprogramming of human fibroblasts into induced hepatocytes, which exhibit metabolic properties similar to primary hepatocytes. ..
  6. pmc Inhibition of activin/nodal signalling is necessary for pancreatic differentiation of human pluripotent stem cells
    C H H Cho
    Wellcome Trust Medical Research Council Cambridge Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine, West Forvie Site, Robinson Way, Cambridge, UK
    Diabetologia 55:3284-95. 2012
    ..However, developing methods to differentiate hESCs/hIPSCs into specific cell types following a natural pathway of development remains a major challenge...
  7. pmc Integration-deficient lentivectors: an effective strategy to purify and differentiate human embryonic stem cell-derived hepatic progenitors
    Guanghua Yang
    INSERM U 972, IFR 93, Bicetre Hospital, and Paul Brousse Hospital, Villejuif F 94807, France
    BMC Biol 11:86. 2013
    ..To date, purification of progenitors and mature cells generated from either embryonic or induced pluripotent stem cells remains challenging with use of conventional methods...
  8. doi Signaling pathways controlling pluripotency and early cell fate decisions of human induced pluripotent stem cells
    Ludovic Vallier
    Laboratory for Regenerative Medicine, University of Cambridge, Cambridge CB2 0SZ, United Kingdom
    Stem Cells 27:2655-66. 2009
    ..Together these data reveal that human iPSCs rely on mechanisms similar to human ESCs to maintain their pluripotency and to control their differentiation, showing that these pluripotent cell types are functionally equivalent...
  9. pmc Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways
    Ludovic Vallier
    Department of Surgery and Laboratory For Regenerative Medicine, University of Cambridge, Cambridge, UK
    PLoS ONE 4:e6082. 2009
    ....
  10. pmc Activin/Nodal signalling maintains pluripotency by controlling Nanog expression
    Ludovic Vallier
    Department of Surgery and Laboratory For Regenerative Medicine, West Forvie Building, Robinson Way, University of Cambridge, Cambridge CB2 0SZ, UK
    Development 136:1339-49. 2009
    ..This negative-feedback loop imposes stasis in neuroectoderm and mesendoderm differentiation, thereby maintaining the pluripotent status of human ESCs and mouse EpiSCs...
  11. ncbi Conditional gene expression in human embryonic stem cells
    Ludovic Vallier
    Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Hills Road, Cambridge CB2 0XY, United Kingdom
    Stem Cells 25:1490-7. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  12. ncbi Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal default pathway
    Ludovic Vallier
    Department of Surgery, University of Cambridge, Cambridge CB2 2QQ, United Kingdom
    Dev Biol 275:403-21. 2004
    ..The effects of Nodal on early differentiation illustrate how hESCs can augment mouse embryos as a model for analyzing mechanisms of early mammalian development...
  13. ncbi Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells
    Ludovic Vallier
    Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
    J Cell Sci 118:4495-509. 2005
    ....
  14. pmc Multiple roles of Activin/Nodal, bone morphogenetic protein, fibroblast growth factor and Wnt/β-catenin signalling in the anterior neural patterning of adherent human embryonic stem cell cultures
    Giuseppe Lupo
    Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK
    Open Biol 3:120167. 2013
    ..These results show that the key mechanisms controlling neural patterning in model vertebrate species are preserved in adherent, chemically defined hESC cultures and reveal new insights into the signals regulating eye field specification...
  15. doi Activin/Nodal signaling controls divergent transcriptional networks in human embryonic stem cells and in endoderm progenitors
    Stephanie Brown
    The Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, United Kingdom
    Stem Cells 29:1176-85. 2011
    ....
  16. ncbi Derivation of pluripotent epiblast stem cells from mammalian embryos
    I Gabrielle M Brons
    Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 0XY, UK
    Nature 448:191-5. 2007
    ..Epiblast stem cells provide a valuable experimental system for determining whether distinctions between mouse and human embryonic stem cells reflect species differences or diverse temporal origins...
  17. ncbi Status of genomic imprinting in epigenetically distinct pluripotent stem cells
    Bowen Sun
    The Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, United Kingdom
    Stem Cells 30:161-8. 2012
    ..Our results also reinforce the view that hESCs and EpiSCs are in vitro counterparts, sharing an epigenetic status distinct from ESCs and iPSCs...
  18. pmc Pluripotency factors regulate definitive endoderm specification through eomesodermin
    Adrian Kee Keong Teo
    Laboratory for Regenerative Medicine, University of Cambridge, Cambridge, UK
    Genes Dev 25:238-50. 2011
    ..Together, these results provide for the first time a comprehensive molecular model connecting the transition from pluripotency to endoderm specification during mammalian development...
  19. pmc Production of hepatocyte-like cells from human pluripotent stem cells
    Nicholas R F Hannan
    Wellcome Trust Medical Research Council Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, UK
    Nat Protoc 8:430-7. 2013
    ..This approach works efficiently with human embryonic stem cells and human induced pluripotent stem cells and was recently used to model liver diseases in vitro...
  20. pmc A practical and efficient cellular substrate for the generation of induced pluripotent stem cells from adults: blood-derived endothelial progenitor cells
    Imbisaat Geti
    University of Cambridge, Cambridge, UK
    Stem Cells Transl Med 1:855-65. 2012
    ..This work identifies L-EPCs as a practical and efficient cellular substrate for iPSC generation, with the potential to address many of the factors currently limiting the translation of this technology...
  21. pmc Genomic targets of Brachyury (T) in differentiating mouse embryonic stem cells
    Amanda L Evans
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 7:e33346. 2012
    ..Work in the frog and the zebrafish has identified some direct genomic targets of Brachyury, but little is known about Brachyury targets in the mouse...
  22. ncbi HOXB4 overexpression promotes hematopoietic development by human embryonic stem cells
    Kristian M Bowles
    Department of Surgery, University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 2XY, UK
    Stem Cells 24:1359-69. 2006
    ....
  23. doi Differentiation of human embryonic stem cells in adherent and in chemically defined culture conditions
    Ludovic Vallier
    Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge, United Kingdom
    Curr Protoc Stem Cell Biol . 2008
    ....
  24. ncbi The cell-cycle state of stem cells determines cell fate propensity
    Siim Pauklin
    Wellcome Trust Medical Research Council Cambridge Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine and Department of Surgery, University of Cambridge, Cambridge CB2 0SZ, UK Electronic address
    Cell 155:135-47. 2013
    ..Our results demonstrate that cell fate decisions are tightly associated with the cell-cycle machinery and reveal insights in the mechanisms synchronizing differentiation and proliferation in developing tissues. ..
  25. ncbi Genetic background drives transcriptional variation in human induced pluripotent stem cells
    Foad Rouhani
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    PLoS Genet 10:e1004432. 2014
    ..Our data also suggest that future studies using hIPSCs as a model system should focus most effort on collection of large numbers of donors, rather than generating large numbers of lines from the same donor. ..
  26. doi Emerging use of stem cells in regenerative medicine
    Adrian K K Teo
    Laboratory for Regenerative Medicine, University of Cambridge, West Forvie Building, Forvie Site, Robinson Way, Cambridge CB2 0SZ, UK
    Biochem J 428:11-23. 2010
    ..The present review will attempt to summarize the advantages and challenges of each type of stem cell for current and future clinical applications using specific examples...
  27. pmc Transplantation of expanded fetal intestinal progenitors contributes to colon regeneration after injury
    Robert P Fordham
    Wellcome Trust and Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, CB2 1QR, UK Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, CB2 0SZ, UK
    Cell Stem Cell 13:734-44. 2013
    ..This work provides insight into mechanisms underlying development of the mammalian intestine and points to future opportunities for patient-specific regeneration of the digestive tract...
  28. ncbi The serpinopathies studying serpin polymerization in vivo
    James A Irving
    Department of Medicine, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    Methods Enzymol 501:421-66. 2011
    ..We describe here the biochemical techniques, monoclonal antibodies, cell biology, animal models, and stem cell technology that are useful to characterize the serpin polymers that form in vivo...
  29. doi Serum-free and feeder-free culture conditions for human embryonic stem cells
    Ludovic Vallier
    Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge, UK
    Methods Mol Biol 690:57-66. 2011
    ..Here, we describe the basic principles of growing hESCs in a chemically defined medium (CDM) devoid of serum and feeders...
  30. doi Induced pluripotent stem cells--alchemist's tale or clinical reality?
    S Tamir Rashid
    Laboratory for Regenerative Medicine, University of Cambridge, Cambridge, UK
    Expert Rev Mol Med 12:25. 2010
    ..In this review, we give an account of the historical development, current technologies and potential clinical applications of induced pluripotency and conclude with a perspective on the possible future directions for this dynamic field...
  31. pmc Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells
    S Tamir Rashid
    Laboratory for Regenerative Medicine, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
    J Clin Invest 120:3127-36. 2010
    ..These patient-derived hepatocytes demonstrate that it is possible to model diseases whose phenotypes are caused by pathological dysregulation of key processes within adult cells...
  32. ncbi Human stem cells for craniomaxillofacial reconstruction
    Morteza Jalali
    1 Anne Mclaren Laboratory for Regenerative Medicine, Wellcome Trust Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
    Stem Cells Dev 23:1437-51. 2014
    ..Here, we provide an overview of human stem cells in disease modeling, drug screening, and therapeutics, while also discussing the application of regenerative medicine for craniomaxillofacial tissue deficit and surgical reconstruction. ..
  33. doi Production of hepatocyte-like cells from human pluripotent stem cells
    Nicholas R F Hannan
    Wellcome Trust Medical Research Council Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, UK
    Nat Protoc 8:430-7. 2013
    ..This approach works efficiently with human embryonic stem cells and human induced pluripotent stem cells and was recently used to model liver diseases in vitro...
  34. ncbi Maturation of induced pluripotent stem cell derived hepatocytes by 3D-culture
    Richard L Gieseck
    Wellcome Trust Medical Research Council Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, United Kingdom Immunopathogenesis Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 9:e86372. 2014
    ..Overall, this research reveals a method to shift the phenotype of existing IPSC-Heps towards primary adult hepatocytes allowing such cells to be a more relevant replacement for the current primary standard. ..
  35. ncbi Investigating the feasibility of scale up and automation of human induced pluripotent stem cells cultured in aggregates in feeder free conditions
    Filipa A C Soares
    Wellcome Trust Medical Research Council Cambridge Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine and Department of Surgery, University of Cambridge, UK Centre for Biological Engineering, Loughborough University, UK Electronic address
    J Biotechnol 173:53-8. 2014
    ..Cellular morphology, pluripotency gene expression and differentiation into the three germ layers have been used compare the outcomes of manual and automated processes. ..
  36. pmc Real-time PCR mapping of DNaseI-hypersensitive sites using a novel ligation-mediated amplification technique
    George A Follows
    Department of Haematology, Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 2XY, UK
    Nucleic Acids Res 35:e56. 2007
    ..Presented examples include comparative DHS mapping of known TAL1/SCL regulatory elements between human embryonic stem cells and K562 cells...
  37. ncbi Navigating the pathway from embryonic stem cells to beta cells
    Markus Stoffel
    Laboratory of Metabolic Diseases, The Rockefeller University, New York, NY 10021, USA
    Semin Cell Dev Biol 15:327-36. 2004
    ....
  38. ncbi Pleiotrophin enhances clonal growth and long-term expansion of human embryonic stem cells
    Boon Seng Soh
    Stem Cell and Developmental Biology, Genome Institute of Singapore, Singapore
    Stem Cells 25:3029-37. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...