Research Topics
Genomes and Genes | Ludovic VallierSummaryAffiliation: University of Cambridge Country: UK Publications
| Collaborators
|
Detail Information
Publications
Enhancing and diminishing gene function in human embryonic stem cellsLudovic Vallier
Department of Surgery, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
Stem Cells 22:2-11. 2004..Application of these gain- and loss-of-function approaches should have widespread use, not only in revealing the developmental roles of specific human genes, but also for their utility in modulating differentiation...- Human embryonic stem cells: an in vitro model to study mechanisms controlling pluripotency in early mammalian developmentLudovic Vallier
Department of Surgery and Cambride Institute for Medical Research Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 2XY, UK
Stem Cell Rev 1:119-30. 2005..With the new perspective of these findings, we suggest a previously unrecognized role of TGF-beta pathway signaling in maintaining pluripotency at early stages of mammalian embryonic development... - Inhibition of Activin/Nodal signaling promotes specification of human embryonic stem cells into neuroectodermJoseph R Smith
Department of Surgery and Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
Dev Biol 313:107-17. 2008..These results show that inhibition of Nodal signaling promotes neuronal specification, indicating a role for this pathway in controlling early neural development of pluripotent cells... 
SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cellsZhenzhi Chng
Laboratory for Regenerative Medicine, West Forvie Building, Robinson Way, University of Cambridge, Cambridge, CB2 0SZ, UK
Cell Stem Cell 6:59-70. 2010....- Signaling pathways controlling pluripotency and early cell fate decisions of human induced pluripotent stem cellsLudovic Vallier
Laboratory for Regenerative Medicine, University of Cambridge, Cambridge CB2 0SZ, United Kingdom
Stem Cells 27:2655-66. 2009..Together these data reveal that human iPSCs rely on mechanisms similar to human ESCs to maintain their pluripotency and to control their differentiation, showing that these pluripotent cell types are functionally equivalent... 
Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathwaysLudovic Vallier
Department of Surgery and Laboratory For Regenerative Medicine, University of Cambridge, Cambridge, UK
PLoS ONE 4:e6082. 2009....- Activin/Nodal signalling maintains pluripotency by controlling Nanog expressionLudovic Vallier
Department of Surgery and Laboratory For Regenerative Medicine, West Forvie Building, Robinson Way, University of Cambridge, Cambridge CB2 0SZ, UK
Development 136:1339-49. 2009..This negative-feedback loop imposes stasis in neuroectoderm and mesendoderm differentiation, thereby maintaining the pluripotent status of human ESCs and mouse EpiSCs... - Conditional gene expression in human embryonic stem cellsLudovic Vallier
Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Hills Road, Cambridge CB2 0XY, United Kingdom
Stem Cells 25:1490-7. 2007..Disclosure of potential conflicts of interest is found at the end of this article... - Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal default pathwayLudovic Vallier
Department of Surgery, University of Cambridge, Cambridge CB2 2QQ, United Kingdom
Dev Biol 275:403-21. 2004..The effects of Nodal on early differentiation illustrate how hESCs can augment mouse embryos as a model for analyzing mechanisms of early mammalian development... - Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cellsLudovic Vallier
Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
J Cell Sci 118:4495-509. 2005.... - Activin/Nodal signaling controls divergent transcriptional networks in human embryonic stem cells and in endoderm progenitorsStephanie Brown
The Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, United Kingdom
Stem Cells 29:1176-85. 2011.... - Status of genomic imprinting in epigenetically distinct pluripotent stem cellsBowen Sun
The Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, United Kingdom
Stem Cells 30:161-8. 2012..Our results also reinforce the view that hESCs and EpiSCs are in vitro counterparts, sharing an epigenetic status distinct from ESCs and iPSCs... - Pluripotency factors regulate definitive endoderm specification through eomesoderminAdrian Kee Keong Teo
Laboratory for Regenerative Medicine, University of Cambridge, Cambridge, UK
Genes Dev 25:238-50. 2011..Together, these results provide for the first time a comprehensive molecular model connecting the transition from pluripotency to endoderm specification during mammalian development... - Derivation of pluripotent epiblast stem cells from mammalian embryosI Gabrielle M Brons
Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 0XY, UK
Nature 448:191-5. 2007..Epiblast stem cells provide a valuable experimental system for determining whether distinctions between mouse and human embryonic stem cells reflect species differences or diverse temporal origins... - Multiple roles of Activin/Nodal, bone morphogenetic protein, fibroblast growth factor and Wnt/β-catenin signalling in the anterior neural patterning of adherent human embryonic stem cell culturesGiuseppe Lupo
Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK
Open Biol 3:120167. 2013..These results show that the key mechanisms controlling neural patterning in model vertebrate species are preserved in adherent, chemically defined hESC cultures and reveal new insights into the signals regulating eye field specification... - A practical and efficient cellular substrate for the generation of induced pluripotent stem cells from adults: blood-derived endothelial progenitor cellsImbisaat Geti
University of Cambridge, Cambridge, UK
Stem Cells Transl Med 1:855-65. 2012..This work identifies L-EPCs as a practical and efficient cellular substrate for iPSC generation, with the potential to address many of the factors currently limiting the translation of this technology... - Genomic targets of Brachyury (T) in differentiating mouse embryonic stem cellsAmanda L Evans
Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, United Kingdom
PLoS ONE 7:e33346. 2012..Work in the frog and the zebrafish has identified some direct genomic targets of Brachyury, but little is known about Brachyury targets in the mouse... - HOXB4 overexpression promotes hematopoietic development by human embryonic stem cellsKristian M Bowles
Department of Surgery, University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 2XY, UK
Stem Cells 24:1359-69. 2006.... - Differentiation of human embryonic stem cells in adherent and in chemically defined culture conditionsLudovic Vallier
Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge, United Kingdom
Curr Protoc Stem Cell Biol . 2008.... - Production of hepatocyte-like cells from human pluripotent stem cellsNicholas R F Hannan
Wellcome Trust Medical Research Council Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, UK
Nat Protoc 8:430-7. 2013..This approach works efficiently with human embryonic stem cells and human induced pluripotent stem cells and was recently used to model liver diseases in vitro... - The serpinopathies studying serpin polymerization in vivoJames A Irving
Department of Medicine, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
Methods Enzymol 501:421-66. 2011..We describe here the biochemical techniques, monoclonal antibodies, cell biology, animal models, and stem cell technology that are useful to characterize the serpin polymers that form in vivo... - Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cellsS Tamir Rashid
Laboratory for Regenerative Medicine, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
J Clin Invest 120:3127-36. 2010..These patient-derived hepatocytes demonstrate that it is possible to model diseases whose phenotypes are caused by pathological dysregulation of key processes within adult cells... - Emerging use of stem cells in regenerative medicineAdrian K K Teo
Laboratory for Regenerative Medicine, University of Cambridge, West Forvie Building, Forvie Site, Robinson Way, Cambridge CB2 0SZ, UK
Biochem J 428:11-23. 2010..The present review will attempt to summarize the advantages and challenges of each type of stem cell for current and future clinical applications using specific examples... - Serum-free and feeder-free culture conditions for human embryonic stem cellsLudovic Vallier
Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge, UK
Methods Mol Biol 690:57-66. 2011..Here, we describe the basic principles of growing hESCs in a chemically defined medium (CDM) devoid of serum and feeders... - Induced pluripotent stem cells--alchemist's tale or clinical reality?S Tamir Rashid
Laboratory for Regenerative Medicine, University of Cambridge, Cambridge, UK
Expert Rev Mol Med 12:25. 2010..In this review, we give an account of the historical development, current technologies and potential clinical applications of induced pluripotency and conclude with a perspective on the possible future directions for this dynamic field... - Production of hepatocyte-like cells from human pluripotent stem cellsNicholas R F Hannan
Wellcome Trust Medical Research Council Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, UK
Nat Protoc 8:430-7. 2013..This approach works efficiently with human embryonic stem cells and human induced pluripotent stem cells and was recently used to model liver diseases in vitro... - Real-time PCR mapping of DNaseI-hypersensitive sites using a novel ligation-mediated amplification techniqueGeorge A Follows
Department of Haematology, Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 2XY, UK
Nucleic Acids Res 35:e56. 2007..Presented examples include comparative DHS mapping of known TAL1/SCL regulatory elements between human embryonic stem cells and K562 cells... - Navigating the pathway from embryonic stem cells to beta cellsMarkus Stoffel
Laboratory of Metabolic Diseases, The Rockefeller University, New York, NY 10021, USA
Semin Cell Dev Biol 15:327-36. 2004.... - Pleiotrophin enhances clonal growth and long-term expansion of human embryonic stem cellsBoon Seng Soh
Stem Cell and Developmental Biology, Genome Institute of Singapore, Singapore
Stem Cells 25:3029-37. 2007..Disclosure of potential conflicts of interest is found at the end of this article...