N J Turner

Summary

Affiliation: University of Edinburgh
Country: UK

Publications

  1. ncbi request reprint Applications of transketolases in organic synthesis
    N J Turner
    Department of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JJ, UK
    Curr Opin Biotechnol 11:527-31. 2000
  2. ncbi request reprint Enzyme catalysed deracemisation and dynamic kinetic resolution reactions
    Nicholas J Turner
    School of Chemistry, University of Edinburgh, UK
    Curr Opin Chem Biol 8:114-9. 2004
  3. ncbi request reprint Directed evolution of enzymes for applied biocatalysis
    Nicholas J Turner
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JJ, Scotland, UK
    Trends Biotechnol 21:474-8. 2003
  4. ncbi request reprint Controlling chirality
    Nicholas J Turner
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, EH9 3JJ, UK
    Curr Opin Biotechnol 14:401-6. 2003
  5. ncbi request reprint Tuning lipase enantioselectivity in organic media using solid-state buffers
    M Quiros
    Department of Chemistry, University of Glasgow, Glasgow, G12 8QQ, UK
    J Org Chem 66:5074-9. 2001
  6. ncbi request reprint Preparative deracemization of unnatural amino acids
    I Fotheringham
    Ingenza Ltd, Joseph Black Building, Kings Buildings, West Mains Road, Edinburgh EH9 3JJ, Scotland, UK
    Biochem Soc Trans 34:287-90. 2006
  7. ncbi request reprint The desymmetrization of bicyclic beta -diketones by an enzymatic retro-Claisen reaction. A new reaction of the crotonase superfamily
    G Grogan
    Edinburgh Centre for Protein Technology, Department of Chemistry, University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh EH9 3JJ, United Kingdom
    J Biol Chem 276:12565-72. 2001
  8. ncbi request reprint Directed evolution of an amine oxidase for the preparative deracemisation of cyclic secondary amines
    Reuben Carr
    School of Chemistry, The University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JJ, Scotland, UK
    Chembiochem 6:637-9. 2005
  9. ncbi request reprint Efficient terpene hydroxylation catalysts based upon P450 enzymes derived from actinomycetes
    Ayhan Celik
    School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JJ, UK
    Org Biomol Chem 3:2930-4. 2005
  10. ncbi request reprint Directed evolution of enzymes: new biocatalysts for asymmetric synthesis
    Marina Alexeeva
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, UK EH9 3JJ
    Org Biomol Chem 1:4133-7. 2003

Collaborators

  • Gianfranco Gilardi
  • I Fotheringham
  • G A Roberts
  • R V Ulijn
  • Veronique Hannaert
  • Thomas A Kost
  • Matthew D Truppo
  • Martin A Wear
  • L P Wackett
  • Reuben Carr
  • Marina Alexeeva
  • Cara E Humphrey
  • Ayhan Celik
  • Colin J Dunsmore
  • Michael J Dawson
  • Sabine L Flitsch
  • Matthew W Nowicki
  • Franck Escalettes
  • Malcolm D Walkinshaw
  • Yuande Yang
  • Robert E Speight
  • Morag A M Easson
  • Alexis Enright
  • Roger J Lins
  • Tony Cass
  • M Quiros
  • G Grogan
  • Liam Worralll
  • Paul A M Michels
  • Lindsay B Tulloch
  • Linda A Fothergill-Gilmore
  • Iain W McNae
  • George Kontopidis
  • Kirk Malone
  • Antony P Page
  • Paul Taylor
  • Elizabeth Moir
  • Toni Fleming
  • Davide Sperandio
  • Vicente Gotor-Fern├índez
  • Tom S C Eve
  • Patrick Condreay
  • Ed Irving
  • Jeroen Hugenholtz
  • Mahmoud Mahmoudian
  • Steven Projan
  • Jim Dunwell
  • Stuart A Brown
  • Michiel Kleerebezem
  • S L Flitsch
  • D Bougioukou
  • M C Parker

Detail Information

Publications23

  1. ncbi request reprint Applications of transketolases in organic synthesis
    N J Turner
    Department of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JJ, UK
    Curr Opin Biotechnol 11:527-31. 2000
    ..X-ray structural studies have been used to probe the origin of the asymmetry of the carbon-carbon bond-forming process...
  2. ncbi request reprint Enzyme catalysed deracemisation and dynamic kinetic resolution reactions
    Nicholas J Turner
    School of Chemistry, University of Edinburgh, UK
    Curr Opin Chem Biol 8:114-9. 2004
    ..In some examples of dynamic kinetic resolution, a second enzyme (racemase) is used to interconvert the enantiomers of the starting material...
  3. ncbi request reprint Directed evolution of enzymes for applied biocatalysis
    Nicholas J Turner
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JJ, Scotland, UK
    Trends Biotechnol 21:474-8. 2003
    ....
  4. ncbi request reprint Controlling chirality
    Nicholas J Turner
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, EH9 3JJ, UK
    Curr Opin Biotechnol 14:401-6. 2003
    ..Moreover, a much greater understanding is being developed of the parameters that can affect the stereochemical outcome of the reaction (e.g. solvent, substrate design, immobilization and directed evolution)...
  5. ncbi request reprint Tuning lipase enantioselectivity in organic media using solid-state buffers
    M Quiros
    Department of Chemistry, University of Glasgow, Glasgow, G12 8QQ, UK
    J Org Chem 66:5074-9. 2001
    ..Significant differences to both the enantioselectivity and catalytic rate were observed, especially under hydrated conditions where byproduct acid was formed...
  6. ncbi request reprint Preparative deracemization of unnatural amino acids
    I Fotheringham
    Ingenza Ltd, Joseph Black Building, Kings Buildings, West Mains Road, Edinburgh EH9 3JJ, Scotland, UK
    Biochem Soc Trans 34:287-90. 2006
    ..We have developed efficient methods to adapt the process towards new target compounds and to optimize key factors that influence process efficiency and offer competitive economics at scale...
  7. ncbi request reprint The desymmetrization of bicyclic beta -diketones by an enzymatic retro-Claisen reaction. A new reaction of the crotonase superfamily
    G Grogan
    Edinburgh Centre for Protein Technology, Department of Chemistry, University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh EH9 3JJ, United Kingdom
    J Biol Chem 276:12565-72. 2001
    ..A mechanism for 6-oxocamphor hydrolase based on sequence homology and the known mechanism of the crotonase enzymes is proposed...
  8. ncbi request reprint Directed evolution of an amine oxidase for the preparative deracemisation of cyclic secondary amines
    Reuben Carr
    School of Chemistry, The University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JJ, Scotland, UK
    Chembiochem 6:637-9. 2005
  9. ncbi request reprint Efficient terpene hydroxylation catalysts based upon P450 enzymes derived from actinomycetes
    Ayhan Celik
    School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JJ, UK
    Org Biomol Chem 3:2930-4. 2005
    ..Both substrates are hydroxylated with a high degree of regioselectivity and for alpha-ionone 1 the reaction is highly diastereoselective yielding the anti-isomer...
  10. ncbi request reprint Directed evolution of enzymes: new biocatalysts for asymmetric synthesis
    Marina Alexeeva
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, UK EH9 3JJ
    Org Biomol Chem 1:4133-7. 2003
    ..Directed evolution has been employed to generate new enzymes for the deracemisation of chiral amines...
  11. ncbi request reprint Lipase-catalyzed kinetic resolution on solid-phase via a "capture and release" strategy
    Cara E Humphrey
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, Scotland, UK
    J Am Chem Soc 125:13952-3. 2003
    ..The (R)-acyl group was subsequently released from the resin by NaOH hydrolysis, and the yield of the reaction could be improved by triple acylation of the resin...
  12. doi request reprint Directed evolution of galactose oxidase: generation of enantioselective secondary alcohol oxidases
    Franck Escalettes
    School of Chemistry, University of Manchester, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester M1 7DN, UK
    Chembiochem 9:857-60. 2008
  13. ncbi request reprint A surface plasmon resonance-based assay for small molecule inhibitors of human cyclophilin A
    Martin A Wear
    Institute of Structural and Molecular Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JR, UK
    Anal Biochem 345:214-26. 2005
    ..53+/-0.1 microM(-1) s(-1) and 1.2+/-0.1 (x 10(-2)) s(-1), respectively. These values agree well with the values for the corresponding binding constants determined from steady-state and kinetic fluorescence titrations in solution...
  14. ncbi request reprint Structure-based discovery of a family of synthetic cyclophilin inhibitors showing a cyclosporin-A phenotype in Caenorhabditis elegans
    Yuande Yang
    The Centre for Translational and Chemical Biology, The University of Edinburgh, Michael Swann Building, King s Buildings, Edinburgh EH9 3JR, UK
    Biochem Biophys Res Commun 363:1013-9. 2007
    ..These dimedone analogues provide a new scaffold for the synthesis of families of peptidomimetic molecules with potential activity against HIV, malaria, and helminth parasite infections...
  15. doi request reprint Rapid determination of both the activity and enantioselectivity of ketoreductases
    Matthew D Truppo
    School of Chemistry, University of Manchester, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester M17DN, UK
    Angew Chem Int Ed Engl 47:2639-41. 2008
  16. ncbi request reprint A chemo-enzymatic route to enantiomerically pure cyclic tertiary amines
    Colin J Dunsmore
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, EH9 3JJ, U K
    J Am Chem Soc 128:2224-5. 2006
    ..Deracemization of racemic chiral tertiary amines has been achieved by combination of an enantioselective amine oxidase, obtained through directed evolution, and ammonia borane in a one-pot process...
  17. ncbi request reprint Enantioselective epoxidation of linolenic acid catalysed by cytochrome P450(BM3) from Bacillus megaterium
    Ayhan Celik
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, UK EH9 3JJ
    Org Biomol Chem 3:2688-90. 2005
    ..The absolute configuration of the product is tentatively assigned as 15(R),16(S)-. The Michaelis-Menten parameters kcat and Km for the reaction were determined to be 3126 +/- 226 min(-1) and 24 +/- 6 microM respectively...
  18. ncbi request reprint Deracemization of alpha-methylbenzylamine using an enzyme obtained by in vitro evolution
    Marina Alexeeva
    Department of Chemistry, Centre for Protein Technology, The University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JJ, UK
    Angew Chem Int Ed Engl 41:3177-80. 2002
  19. ncbi request reprint Dimedone esters as novel hydrolase substrates and their application in the colorimetric detection of lipase and esterase activity
    Cara E Humphrey
    School of Chemistry, The University of Edinburgh, Kings Buildings, West Mains Road, Edinburgh EH9 3JJ, UK
    Chembiochem 5:1144-8. 2004
  20. ncbi request reprint Directed evolution of an amine oxidase possessing both broad substrate specificity and high enantioselectivity
    Reuben Carr
    School of Chemistry, The University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JJ, UK
    Angew Chem Int Ed Engl 42:4807-10. 2003
  21. ncbi request reprint Biotechnology. Paper Alert
    Tony Cass
    Imperial College of Science, Technology and Medicine, London, UK
    Curr Opin Biotechnol 13:1-8. 2002
    ..A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in biotechnology...
  22. ncbi request reprint Enzymatic generation and in situ screening of a dynamic combinatorial library of sialic acid analogues
    Roger J Lins
    Department of Chemistry, Edinburgh Protein Interaction Centre, The University of Edinburgh, King s Buildings West Mains Road, Edinburgh EH9 3JJ, UK
    Angew Chem Int Ed Engl 41:3405-7. 2002
  23. doi request reprint Design, synthesis and trypanocidal activity of lead compounds based on inhibitors of parasite glycolysis
    Matthew W Nowicki
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, EH9 3JJ, UK
    Bioorg Med Chem 16:5050-61. 2008
    ..Trypanocidal activity also showed potency in the low micromolar range and confirms these inhibitors as promising candidates for the development towards the design of anti-trypanosomal drugs...