Alan Tunnacliffe

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. pmc Multiple functionally divergent and conserved copies of alpha tubulin in bdelloid rotifers
    Isobel Eyres
    Department of Life Sciences, Imperial College London, Silwood Park Campus, Ascot, Berkshire, SL5 7PY, UK
    BMC Evol Biol 12:148. 2012
  2. ncbi request reprint The continuing conundrum of the LEA proteins
    Alan Tunnacliffe
    Institute of Biotechnology, University of Cambridge, Cambridge, UK
    Naturwissenschaften 94:791-812. 2007
  3. doi request reprint Trafficking of bdelloid rotifer late embryogenesis abundant proteins
    Rashmi Tripathi
    Cell and Organism Engineering Laboratory, Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge CB2 3RA, UK
    J Exp Biol 215:2786-94. 2012
  4. doi request reprint Intrinsically disordered proteins as molecular shields
    Sohini Chakrabortee
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    Mol Biosyst 8:210-9. 2012
  5. pmc Hydrophilic protein associated with desiccation tolerance exhibits broad protein stabilization function
    Sohini Chakrabortee
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, United Kingdom
    Proc Natl Acad Sci U S A 104:18073-8. 2007
  6. pmc Biochemical diversification through foreign gene expression in bdelloid rotifers
    Chiara Boschetti
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    PLoS Genet 8:e1003035. 2012
  7. doi request reprint Foreign genes and novel hydrophilic protein genes participate in the desiccation response of the bdelloid rotifer Adineta ricciae
    Chiara Boschetti
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    J Exp Biol 214:59-68. 2011
  8. doi request reprint Critique of the use of fluorescence-based reporters in Escherichia coli as a screening tool for the identification of peptide inhibitors of Aβ42 aggregation
    Oliver Wright
    Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK
    J Pept Sci 19:74-83. 2013
  9. ncbi request reprint Functional divergence of former alleles in an ancient asexual invertebrate
    Natalia N Pouchkina-Stantcheva
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    Science 318:268-71. 2007
  10. doi request reprint Phenomic and transcriptomic analyses reveal that autophagy plays a major role in desiccation tolerance in Saccharomyces cerevisiae
    Sooraj Ratnakumar
    Department of Chemical Engineering and Biotechnology, Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    Mol Biosyst 7:139-49. 2011

Collaborators

  • Michael J Wise
  • Yun Liu
  • David C Rubinsztein
  • Esther Lubzens
  • Timothy G Barraclough
  • Stephen G Oliver
  • Elaine Stephens
  • Andrew Hayes
  • Rakesh K Rastogi
  • Filip Meersman
  • Sovan Sarkar
  • Andy Hesketh
  • Chiara Boschetti
  • Sohini Chakrabortee
  • Oliver Wright
  • Zebo Huang
  • Sooraj Ratnakumar
  • Rashmi Tripathi
  • Isobel Eyres
  • Natalia N Pouchkina-Stantcheva
  • Tatsuya Yoshimi
  • Liao Zhang
  • Yizhi Zheng
  • Matthew C Banton
  • Kshamata Goyal
  • Adrian Carr
  • Gos Micklem
  • Clemens F Kaminski
  • Gabriele S Kaminski Schierle
  • Brian McGee
  • Xiaojuan Wang
  • Maximino Manzanera
  • John A Browne
  • Jens Lapinski
  • John Browne
  • Diego Fontaneto
  • Chun Ru Cheng
  • Davy P Kurniawan
  • Ni Pan
  • Eftychios Frangedakis
  • Helena R Matthews
  • Elisabeth A Herniou
  • Alastair Crisp
  • Matthew Watson
  • De An Guo
  • Shu Hong Guan
  • Hanrui Zhang
  • Yuan Wang-Koh
  • Konstantinos Gkargkas
  • Pia Hoffmann
  • Natalia Pouchkina-Stantcheva
  • Michael Wilson
  • Bharat M Rash
  • Carlos W Bertoncini
  • Elizabeth A H Hall
  • Maria Jose Ruedas-Rama
  • Dimitri Tolleter
  • David Macherel
  • Dirk K Hincha
  • Laura J Walton
  • Brian M McGee
  • Antoaneta V Popova
  • Sarah L Maslen
  • Claudia Pinelli
  • Katharine M Dolan
  • Trevor Tyson
  • Ann M Burnell
  • Susana Vilchez
  • Ann Burnell

Detail Information

Publications25

  1. pmc Multiple functionally divergent and conserved copies of alpha tubulin in bdelloid rotifers
    Isobel Eyres
    Department of Life Sciences, Imperial College London, Silwood Park Campus, Ascot, Berkshire, SL5 7PY, UK
    BMC Evol Biol 12:148. 2012
    ..Results were verified using transcriptome data from one bdelloid species, Adineta ricciae...
  2. ncbi request reprint The continuing conundrum of the LEA proteins
    Alan Tunnacliffe
    Institute of Biotechnology, University of Cambridge, Cambridge, UK
    Naturwissenschaften 94:791-812. 2007
    ..The challenge now facing researchers investigating these enigmatic proteins is to make sense of the various in vitro defined functions in the living cell: Are the LEA proteins truly multi-talented, or are they still just misunderstood?..
  3. doi request reprint Trafficking of bdelloid rotifer late embryogenesis abundant proteins
    Rashmi Tripathi
    Cell and Organism Engineering Laboratory, Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge CB2 3RA, UK
    J Exp Biol 215:2786-94. 2012
    ..We speculate that this mechanism allows a small number of LEA proteins to offer protection to a large number of desiccation-sensitive molecules and structures both inside and outside cells in the bdelloid rotifer...
  4. doi request reprint Intrinsically disordered proteins as molecular shields
    Sohini Chakrabortee
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    Mol Biosyst 8:210-9. 2012
    ..It seems clear therefore that molecular shield function is largely an intermolecular activity implemented by specialist IDPs, distinct from molecular chaperones, but with a role in proteostasis...
  5. pmc Hydrophilic protein associated with desiccation tolerance exhibits broad protein stabilization function
    Sohini Chakrabortee
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, United Kingdom
    Proc Natl Acad Sci U S A 104:18073-8. 2007
    ..Finally, human cells expressing LEA protein exhibit increased survival of dehydration imposed by osmotic upshift, consistent with a broad protein stabilization function of LEA proteins under conditions of water stress...
  6. pmc Biochemical diversification through foreign gene expression in bdelloid rotifers
    Chiara Boschetti
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    PLoS Genet 8:e1003035. 2012
    ..This represents a potential mechanism for ancient asexuals to adapt rapidly to changing environments and thereby persist over long evolutionary time periods in the absence of sex...
  7. doi request reprint Foreign genes and novel hydrophilic protein genes participate in the desiccation response of the bdelloid rotifer Adineta ricciae
    Chiara Boschetti
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    J Exp Biol 214:59-68. 2011
    ....
  8. doi request reprint Critique of the use of fluorescence-based reporters in Escherichia coli as a screening tool for the identification of peptide inhibitors of Aβ42 aggregation
    Oliver Wright
    Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK
    J Pept Sci 19:74-83. 2013
    ..Thus, although biosynthetic in vivo strategies show promise as screens for novel activities, unforeseen problems can arise because of the variability inherent in any biological system...
  9. ncbi request reprint Functional divergence of former alleles in an ancient asexual invertebrate
    Natalia N Pouchkina-Stantcheva
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    Science 318:268-71. 2007
    ..The functional divergence of former alleles observed here suggests that adoption of asexual reproduction could itself be an evolutionary mechanism for the generation of diversity...
  10. doi request reprint Phenomic and transcriptomic analyses reveal that autophagy plays a major role in desiccation tolerance in Saccharomyces cerevisiae
    Sooraj Ratnakumar
    Department of Chemical Engineering and Biotechnology, Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    Mol Biosyst 7:139-49. 2011
    ..Changes in nitrogen metabolism were evident during the drying process and parts of the environmental stress response were activated, repressing ribosome production and inducing genes for coping with oxidative and osmotic stress...
  11. pmc Catalytic and chaperone-like functions in an intrinsically disordered protein associated with desiccation tolerance
    Sohini Chakrabortee
    Department of Chemical Engineering and Biotechnology, Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, United Kingdom
    Proc Natl Acad Sci U S A 107:16084-9. 2010
    ..Anhydrin therefore extends the range of IDP functional categories to include catalysis and highlights the potential for the discovery of new functions in disordered proteomes...
  12. doi request reprint MAPK phosphorylation is implicated in the adaptation to desiccation stress in nematodes
    Matthew C Banton
    Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge CB2 3RA, UK
    J Exp Biol 215:4288-98. 2012
    ..superbus as a fast desiccation strategist and its high level of preparedness for anhydrobiosis in the hydrated state. These findings show that MAPKs play an important role in the survival of organisms during anhydrobiosis...
  13. doi request reprint Macromolecular and small-molecule modulation of intracellular Aβ42 aggregation and associated toxicity
    Sohini Chakrabortee
    Cell and Organism Engineering Laboratory, Department of Chemical Engineering and Biotechnology, University of Cambridge, UK
    Biochem J 442:507-15. 2012
    ..The model is therefore of value to study the effects of intracellular Aβ on cell physiology and to identify reagents that counteract those effects...
  14. ncbi request reprint POPP the question: what do LEA proteins do?
    Michael J Wise
    Department of Genetics, University of Cambridge, Downing Street, CB2 3EH, Cambridge, UK
    Trends Plant Sci 9:13-7. 2004
    ..Predictions arising from bioinformatics fit well with recent data on Group 3 proteins, which potentially form cytoskeletal filaments, and suggest experimentally testable functions for these and other LEA protein groups...
  15. ncbi request reprint Intracellular trehalose is neither necessary nor sufficient for desiccation tolerance in yeast
    Sooraj Ratnakumar
    Institute of Biotechnology, University of Cambridge, Cambridge, UK
    FEMS Yeast Res 6:902-13. 2006
    ..cerevisiae. Trehalose seems to be neither necessary nor sufficient for, although in some strains might quantitatively improve, survival of desiccation, suggesting that other adaptations are more important...
  16. doi request reprint Recombinant production of cathelicidin-derived antimicrobial peptides in Escherichia coli using an inducible autocleaving enzyme tag
    Oliver Wright
    Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge CB2 3RA, UK
    N Biotechnol 29:352-8. 2012
    ..coli, Pseudomonas putida, Bacillus subtilis and Candida albicans. This approach to linear AMP production may aid rapid construction and purification of structural variants for subsequent functional analysis...
  17. doi request reprint Ratiometric pH-dot ANSors
    Xiaojuan Wang
    Institute of Biotechnology, Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK
    Analyst 135:1585-91. 2010
    ..The sensors are stable, robust and capable of measuring pH in the physiologically relevant range...
  18. ncbi request reprint Spliced leader RNA-mediated trans-splicing in phylum Rotifera
    Natalia N Pouchkina-Stantcheva
    Institute of Biotechnology, University of Cambridge, Cambridge, United Kingdom
    Mol Biol Evol 22:1482-9. 2005
    ....
  19. ncbi request reprint Dehydration-regulated processing of late embryogenesis abundant protein in a desiccation-tolerant nematode
    Kshamata Goyal
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    FEBS Lett 579:4093-8. 2005
    ..Processed polypeptides retain function as protein anti-aggregants and we hypothesise that the expression pattern and cleavage of LEA protein allow rapid, maximal availability of active molecules to the dehydrating animal...
  20. ncbi request reprint High survival and stability rates of Escherichia coli dried in hydroxyectoine
    Maximino Manzanera
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    FEMS Microbiol Lett 233:347-52. 2004
    ..This suggests that, although both hydroxyectoine and trehalose perform equally well as extracellular protectants, trehalose is preferred for intracellular protection...
  21. ncbi request reprint Anhydrobiosis without trehalose in bdelloid rotifers
    Jens Lapinski
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, CB2 1QT Cambridge, UK
    FEBS Lett 553:387-90. 2003
    ..This first observation of animal anhydrobiosis without trehalose challenges our current understanding of the phenomenon and calls for a re-evaluation of existing models...
  22. pmc Dehydration-specific induction of hydrophilic protein genes in the anhydrobiotic nematode Aphelenchus avenae
    John A Browne
    Institute of Bioengineering and Agroecology, Department of Biology, National University of Ireland Maynooth, Maynooth, Co Kildare, Ireland
    Eukaryot Cell 3:966-75. 2004
    ....
  23. pmc Response of human cells to desiccation: comparison with hyperosmotic stress response
    Zebo Huang
    Institute of Biotechnology University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    J Physiol 558:181-91. 2004
    ..Our results show for the first time that human cells react rapidly to desiccation by MAPK activation, and that the response partially overlaps with that to hyperosmotic stress...
  24. ncbi request reprint Desiccation response of mammalian cells: anhydrosignaling
    Zebo Huang
    College of Pharmacy, Wuhan University, Wuhan, China
    Methods Enzymol 428:269-77. 2007
    ..This chapter describes some of the methods used to begin to uncover the response to evaporative water loss in human cell cultures...
  25. ncbi request reprint Anhydrobiosis: plant desiccation gene found in a nematode
    John Browne
    Department of Biology and Institute of Bioengineering and Agroecology, National University of Ireland Maynooth, County Kildare, Ireland
    Nature 416:38. 2002
    ..This finding suggests that animals and plants that undergo anhydrobiosis may use common protective strategies against dehydration, and provides a unifying insight into the mechanism of anhydrobiosis...