Research Topics
| Alan TunnacliffeSummaryAffiliation: University of Cambridge Country: UK Publications
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Publications
The continuing conundrum of the LEA proteinsAlan Tunnacliffe
Institute of Biotechnology, University of Cambridge, Cambridge, UK
Naturwissenschaften 94:791-812. 2007..The challenge now facing researchers investigating these enigmatic proteins is to make sense of the various in vitro defined functions in the living cell: Are the LEA proteins truly multi-talented, or are they still just misunderstood?..
Trafficking of bdelloid rotifer late embryogenesis abundant proteinsRashmi Tripathi
Cell and Organism Engineering Laboratory, Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge CB2 3RA, UK
J Exp Biol 215:2786-94. 2012..We speculate that this mechanism allows a small number of LEA proteins to offer protection to a large number of desiccation-sensitive molecules and structures both inside and outside cells in the bdelloid rotifer...- Intrinsically disordered proteins as molecular shieldsSohini Chakrabortee
Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
Mol Biosyst 8:210-9. 2012..It seems clear therefore that molecular shield function is largely an intermolecular activity implemented by specialist IDPs, distinct from molecular chaperones, but with a role in proteostasis... 
Hydrophilic protein associated with desiccation tolerance exhibits broad protein stabilization functionSohini Chakrabortee
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, United Kingdom
Proc Natl Acad Sci U S A 104:18073-8. 2007..Finally, human cells expressing LEA protein exhibit increased survival of dehydration imposed by osmotic upshift, consistent with a broad protein stabilization function of LEA proteins under conditions of water stress...- Foreign genes and novel hydrophilic protein genes participate in the desiccation response of the bdelloid rotifer Adineta ricciaeChiara Boschetti
Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
J Exp Biol 214:59-68. 2011.... - Functional divergence of former alleles in an ancient asexual invertebrateNatalia N Pouchkina Stantcheva
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
Science 318:268-71. 2007..The functional divergence of former alleles observed here suggests that adoption of asexual reproduction could itself be an evolutionary mechanism for the generation of diversity... - Phenomic and transcriptomic analyses reveal that autophagy plays a major role in desiccation tolerance in Saccharomyces cerevisiaeSooraj Ratnakumar
Department of Chemical Engineering and Biotechnology, Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
Mol Biosyst 7:139-49. 2011..Changes in nitrogen metabolism were evident during the drying process and parts of the environmental stress response were activated, repressing ribosome production and inducing genes for coping with oxidative and osmotic stress... - Catalytic and chaperone-like functions in an intrinsically disordered protein associated with desiccation toleranceSohini Chakrabortee
Department of Chemical Engineering and Biotechnology, Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, United Kingdom
Proc Natl Acad Sci U S A 107:16084-9. 2010..Anhydrin therefore extends the range of IDP functional categories to include catalysis and highlights the potential for the discovery of new functions in disordered proteomes... - Macromolecular and small-molecule modulation of intracellular Aβ42 aggregation and associated toxicitySohini Chakrabortee
Cell and Organism Engineering Laboratory, Department of Chemical Engineering and Biotechnology, University of Cambridge, UK
Biochem J 442:507-15. 2012..The model is therefore of value to study the effects of intracellular Aβ on cell physiology and to identify reagents that counteract those effects... - Intracellular trehalose is neither necessary nor sufficient for desiccation tolerance in yeastSooraj Ratnakumar
Institute of Biotechnology, University of Cambridge, Cambridge, UK
FEMS Yeast Res 6:902-13. 2006..cerevisiae. Trehalose seems to be neither necessary nor sufficient for, although in some strains might quantitatively improve, survival of desiccation, suggesting that other adaptations are more important... - POPP the question: what do LEA proteins do?Michael J Wise
Department of Genetics, University of Cambridge, Downing Street, CB2 3EH, Cambridge, UK
Trends Plant Sci 9:13-7. 2004..Predictions arising from bioinformatics fit well with recent data on Group 3 proteins, which potentially form cytoskeletal filaments, and suggest experimentally testable functions for these and other LEA protein groups... - Biochemical diversification through foreign gene expression in bdelloid rotifersChiara Boschetti
Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
PLoS Genet 8:e1003035. 2012..This represents a potential mechanism for ancient asexuals to adapt rapidly to changing environments and thereby persist over long evolutionary time periods in the absence of sex... - Recombinant production of cathelicidin-derived antimicrobial peptides in Escherichia coli using an inducible autocleaving enzyme tagOliver Wright
Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge CB2 3RA, UK
N Biotechnol 29:352-8. 2012..coli, Pseudomonas putida, Bacillus subtilis and Candida albicans. This approach to linear AMP production may aid rapid construction and purification of structural variants for subsequent functional analysis... - Ratiometric pH-dot ANSorsXiaojuan Wang
Institute of Biotechnology, Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK
Analyst 135:1585-91. 2010..The sensors are stable, robust and capable of measuring pH in the physiologically relevant range... - Dehydration-regulated processing of late embryogenesis abundant protein in a desiccation-tolerant nematodeKshamata Goyal
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
FEBS Lett 579:4093-8. 2005..Processed polypeptides retain function as protein anti-aggregants and we hypothesise that the expression pattern and cleavage of LEA protein allow rapid, maximal availability of active molecules to the dehydrating animal... - Spliced leader RNA-mediated trans-splicing in phylum RotiferaNatalia N Pouchkina-Stantcheva
Institute of Biotechnology, University of Cambridge, Cambridge, United Kingdom
Mol Biol Evol 22:1482-9. 2005.... - High survival and stability rates of Escherichia coli dried in hydroxyectoineMaximino Manzanera
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
FEMS Microbiol Lett 233:347-52. 2004..This suggests that, although both hydroxyectoine and trehalose perform equally well as extracellular protectants, trehalose is preferred for intracellular protection... - Anhydrobiosis without trehalose in bdelloid rotifersJens Lapinski
Institute of Biotechnology, University of Cambridge, Tennis Court Road, CB2 1QT Cambridge, UK
FEBS Lett 553:387-90. 2003..This first observation of animal anhydrobiosis without trehalose challenges our current understanding of the phenomenon and calls for a re-evaluation of existing models... - Dehydration-specific induction of hydrophilic protein genes in the anhydrobiotic nematode Aphelenchus avenaeJohn A Browne
Institute of Bioengineering and Agroecology, Department of Biology, National University of Ireland Maynooth, Maynooth, Co. Kildare, Ireland
Eukaryot Cell 3:966-75. 2004.... - Desiccation response of mammalian cells: anhydrosignalingZebo Huang
College of Pharmacy, Wuhan University, Wuhan, China
Methods Enzymol 428:269-77. 2007..This chapter describes some of the methods used to begin to uncover the response to evaporative water loss in human cell cultures... - Response of human cells to desiccation: comparison with hyperosmotic stress responseZebo Huang
Institute of Biotechnology University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
J Physiol 558:181-91. 2004..Our results show for the first time that human cells react rapidly to desiccation by MAPK activation, and that the response partially overlaps with that to hyperosmotic stress... - Anhydrobiosis: plant desiccation gene found in a nematodeJohn Browne
Department of Biology and Institute of Bioengineering and Agroecology, National University of Ireland Maynooth, County Kildare, Ireland
Nature 416:38. 2002..This finding suggests that animals and plants that undergo anhydrobiosis may use common protective strategies against dehydration, and provides a unifying insight into the mechanism of anhydrobiosis...