D T Thwaites

Summary

Affiliation: University of Newcastle
Country: UK

Publications

  1. ncbi request reprint H/dipeptide absorption across the human intestinal epithelium is controlled indirectly via a functional Na/H exchanger
    David T Thwaites
    Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom
    Gastroenterology 122:1322-33. 2002
  2. pmc Human solute carrier SLC6A14 is the beta-alanine carrier
    Catriona M H Anderson
    Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    J Physiol 586:4061-7. 2008
  3. pmc Amino acid derivatives are substrates or non-transported inhibitors of the amino acid transporter PAT2 (slc36a2)
    Noel Edwards
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK
    Biochim Biophys Acta 1808:260-70. 2011
  4. pmc H+-coupled nutrient, micronutrient and drug transporters in the mammalian small intestine
    David T Thwaites
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Exp Physiol 92:603-19. 2007
  5. ncbi request reprint Deciphering the mechanisms of intestinal imino (and amino) acid transport: the redemption of SLC36A1
    David T Thwaites
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Biochim Biophys Acta 1768:179-97. 2007
  6. pmc Gamma-Aminobutyric acid (GABA) transport across human intestinal epithelial (Caco-2) cell monolayers
    D T Thwaites
    Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, U K
    Br J Pharmacol 129:457-64. 2000
  7. pmc H(+)/solute-induced intracellular acidification leads to selective activation of apical Na(+)/H(+) exchange in human intestinal epithelial cells
    D T Thwaites
    Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Clin Invest 104:629-35. 1999
  8. ncbi request reprint H+-zwitterionic amino acid symport at the brush-border membrane of human intestinal epithelial (CACO-2) cells
    D T Thwaites
    Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Exp Physiol 84:275-84. 1999
  9. pmc The SLC36 family of proton-coupled amino acid transporters and their potential role in drug transport
    David T Thwaites
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK
    Br J Pharmacol 164:1802-16. 2011
  10. ncbi request reprint Dual modes of 5-(N-ethyl-N-isopropyl)amiloride modulation of apical dipeptide uptake in the human small intestinal epithelial cell line Caco-2
    D J Kennedy
    Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    Cell Mol Life Sci 62:1621-31. 2005

Collaborators

Detail Information

Publications26

  1. ncbi request reprint H/dipeptide absorption across the human intestinal epithelium is controlled indirectly via a functional Na/H exchanger
    David T Thwaites
    Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom
    Gastroenterology 122:1322-33. 2002
    ..This study aimed to examine whether physiologic regulation of NHE3 activity can limit hPepT1 capacity and, therefore, protein absorption after a meal...
  2. pmc Human solute carrier SLC6A14 is the beta-alanine carrier
    Catriona M H Anderson
    Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    J Physiol 586:4061-7. 2008
    ....
  3. pmc Amino acid derivatives are substrates or non-transported inhibitors of the amino acid transporter PAT2 (slc36a2)
    Noel Edwards
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK
    Biochim Biophys Acta 1808:260-70. 2011
    ..These two tryptophan derivatives should prove valuable experimental tools in future investigations of the physiological roles of PAT2...
  4. pmc H+-coupled nutrient, micronutrient and drug transporters in the mammalian small intestine
    David T Thwaites
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Exp Physiol 92:603-19. 2007
    ....
  5. ncbi request reprint Deciphering the mechanisms of intestinal imino (and amino) acid transport: the redemption of SLC36A1
    David T Thwaites
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Biochim Biophys Acta 1768:179-97. 2007
    ....
  6. pmc Gamma-Aminobutyric acid (GABA) transport across human intestinal epithelial (Caco-2) cell monolayers
    D T Thwaites
    Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, U K
    Br J Pharmacol 129:457-64. 2000
    ..6. These observations demonstrate H+/GABA symport and suggest that this transport mechanism may be accessible as a route for oral absorption of therapeutically-useful GABA analogues...
  7. pmc H(+)/solute-induced intracellular acidification leads to selective activation of apical Na(+)/H(+) exchange in human intestinal epithelial cells
    D T Thwaites
    Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Clin Invest 104:629-35. 1999
    ....
  8. ncbi request reprint H+-zwitterionic amino acid symport at the brush-border membrane of human intestinal epithelial (CACO-2) cells
    D T Thwaites
    Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Exp Physiol 84:275-84. 1999
    ....
  9. pmc The SLC36 family of proton-coupled amino acid transporters and their potential role in drug transport
    David T Thwaites
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK
    Br J Pharmacol 164:1802-16. 2011
    ....
  10. ncbi request reprint Dual modes of 5-(N-ethyl-N-isopropyl)amiloride modulation of apical dipeptide uptake in the human small intestinal epithelial cell line Caco-2
    D J Kennedy
    Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    Cell Mol Life Sci 62:1621-31. 2005
    ..EIPA (at 100 microM) has similar effects, but at higher concentrations (> 200 microM) also has direct inhibitory effects on hPepT1...
  11. ncbi request reprint Indirect regulation of the intestinal H+-coupled amino acid transporter hPAT1 (SLC36A1)
    Catriona M H Anderson
    Faculty of Medical Sciences, Institute for Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom
    J Cell Physiol 204:604-13. 2005
    ..In conclusion, we have shown that amino acid uptake via hPAT1 is inhibited by activators of the cAMP pathway indirectly through inhibition of NHE3 activity...
  12. pmc Substrate upregulation of the human small intestinal peptide transporter, hPepT1
    D Walker
    Department of Physiological Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    J Physiol 507:697-706. 1998
    ..The signalling pathway between increased dietary peptide and hPepT1 upregulation, therefore, involves direct action on the enterocyte, independent of hormonal and/or neural control...
  13. ncbi request reprint Expression and role of sodium, potassium, chloride cotransport (NKCC1) in mouse inner medullary collecting duct (mIMCD-K2) epithelial cells
    M Glanville
    Department of Physiological Sciences, Medical School, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Pflugers Arch 443:123-31. 2001
    ..We conclude that NKCC1 participates in selective uptake of NH4+ at the basal surface, and that IMCD may function in direct NH4+ deposition to urine...
  14. ncbi request reprint Optimal absorptive transport of the dipeptide glycylsarcosine is dependent on functional Na+/H+ exchange activity
    D J Kennedy
    Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Pflugers Arch 445:139-46. 2002
    ..Uptake in hPepT1-expressing oocytes was independent of Na(+) or S1611. We conclude that functional NHE3 activity is required to allow optimal absorption of dipeptides across the human intestinal epithelium...
  15. pmc Angiotensin-converting enzyme (ACE) inhibitor transport in human intestinal epithelial (Caco-2) cells
    D T Thwaites
    Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School
    Br J Pharmacol 114:981-6. 1995
    ..These observations are consistent with the greater oral availability and time-dependent absorption profile of enalapril maleate and captopril, compared to lisinopril...
  16. pmc Vigabatrin transport across the human intestinal epithelial (Caco-2) brush-border membrane is via the H+ -coupled amino-acid transporter hPAT1
    Emily L Abbot
    Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH
    Br J Pharmacol 147:298-306. 2006
    ....
  17. pmc Inhibition of intestinal dipeptide transport by the neuropeptide VIP is an anti-absorptive effect via the VPAC1 receptor in a human enterocyte-like cell line (Caco-2)
    Catriona M H Anderson
    School of Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH
    Br J Pharmacol 138:564-73. 2003
    ..6. These observations demonstrate that VIP (and PACAP) modulate activity of the H(+)/dipeptide transporter hPepT1 in a Na(+)-dependent manner consistent with the modulation being indirect through inhibition of NHE3...
  18. pmc Transport of the photodynamic therapy agent 5-aminolevulinic acid by distinct H+-coupled nutrient carriers coexpressed in the small intestine
    Catriona M H Anderson
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Framlington Place, Newcastle University, Newcastle upon Tyne, UK
    J Pharmacol Exp Ther 332:220-8. 2010
    ..Thus, increased PepT1 expression in colonic cancer could contribute to the increased PpIX accumulation observed. Selective inhibition of PAT1 could enhance PpIX loading in tumor tissue relative to that in normal tissue...
  19. pmc Substrate specificity and functional characterisation of the H+/amino acid transporter rat PAT2 (Slc36a2)
    David J Kennedy
    Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Br J Pharmacol 144:28-41. 2005
    ..The structural characteristics that determine the substrate specificity of rPAT2 have been identified. This information should prove valuable in the design of selective substrates/inhibitors for PAT1 and PAT2...
  20. pmc Taurine uptake across the human intestinal brush-border membrane is via two transporters: H+-coupled PAT1 (SLC36A1) and Na+- and Cl(-)-dependent TauT (SLC6A6)
    Catriona M H Anderson
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
    J Physiol 587:731-44. 2009
    ..PAT1 may be responsible for bulk taurine uptake during a meal whereas TauT may be important for taurine supply to the intestinal epithelium and for taurine capture between meals...
  21. ncbi request reprint H+/amino acid transporter 1 (PAT1) is the imino acid carrier: An intestinal nutrient/drug transporter in human and rat
    Catriona M H Anderson
    Faculty of Medical Sciences, nUniversity of Newcastle Upon Tyne, Newcasttle Upon Tyne, UK
    Gastroenterology 127:1410-22. 2004
    ....
  22. pmc Regulation of intestinal hPepT1 (SLC15A1) activity by phosphodiesterase inhibitors is via inhibition of NHE3 (SLC9A3)
    Catriona M H Anderson
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    Biochim Biophys Acta 1768:1822-9. 2007
    ....
  23. ncbi request reprint Structure, tissue expression pattern, and function of the amino acid transporter rat PAT2
    Zhong Chen
    Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA 30912, USA
    Biochem Biophys Res Commun 304:747-54. 2003
    ..In conclusion PAT2 has a limited tissue distribution, higher affinity (Michaelis-Menten constant for glycine uptake between 0.49 and 0.69mM), and distinct substrate specificity compared to PAT1...
  24. ncbi request reprint Substrate recognition by the mammalian proton-dependent amino acid transporter PAT1
    Michael Boll
    Molecular Nutrition Unit, Technical University of Munich, Hochfeldweg 2, D 85350 Freising Weihenstephan, Germany
    Mol Membr Biol 20:261-9. 2003
    ..A critical recognition criterion of PAT1 is the backbone charge separation distance and the side chain size, whereas substitutions on the amino group are well tolerated...
  25. ncbi request reprint Isolation and function of the amino acid transporter PAT1 (slc36a1) from rabbit and discrimination between transport via PAT1 and system IMINO in renal brush-border membrane vesicles
    Seiji Miyauchi
    Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA, USA
    Mol Membr Biol 22:549-59. 2005
    ..The functional characteristics of rabbit PAT1 in either mammalian cells or renal BBMV suggest that PAT1 is the low-affinity transporter of proline, glycine and hydroxyproline believed to be defective in patients with iminoglycinuria...
  26. pmc Structure, function and immunolocalization of a proton-coupled amino acid transporter (hPAT1) in the human intestinal cell line Caco-2
    Zhong Chen
    Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA 30912, USA
    J Physiol 546:349-61. 2003
    ..These data suggest that hPAT1 is responsible for the H(+)-coupled amino acid transport expressed in the apical membrane of Caco-2 cells...