David J Thornton

Summary

Affiliation: University of Manchester
Country: UK

Publications

  1. ncbi request reprint Structure and function of the polymeric mucins in airways mucus
    David J Thornton
    Wellcome Trust Center for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    Annu Rev Physiol 70:459-86. 2008
  2. pmc Reduced cell proliferation and increased apoptosis are significant pathological mechanisms in a murine model of mild pseudoachondroplasia resulting from a mutation in the C-terminal domain of COMP
    Katarzyna A Piróg-Garcia
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, UK
    Hum Mol Genet 16:2072-88. 2007
  3. ncbi request reprint Calcium-dependent protein interactions in MUC5B provide reversible cross-links in salivary mucus
    Bertrand D E Raynal
    Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, 2 205 Stopford Bldg, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 278:28703-10. 2003
  4. pmc An unfolded protein response is the initial cellular response to the expression of mutant matrilin-3 in a mouse model of multiple epiphyseal dysplasia
    Seema Nundlall
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK
    Cell Stress Chaperones 15:835-49. 2010
  5. pmc Structural and functional characterization of recombinant matrilin-3 A-domain and implications for human genetic bone diseases
    Maryline Fresquet
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 282:34634-43. 2007
  6. doi request reprint Proteomic analysis of polymeric salivary mucins: no evidence for MUC19 in human saliva
    Karine Rousseau
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Manchester M13 9PT, UK
    Biochem J 413:545-52. 2008
  7. pmc Muc5b is the major polymeric mucin in mucus from thoroughbred horses with and without airway mucus accumulation
    Karine Rousseau
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    PLoS ONE 6:e19678. 2011
  8. pmc MUC5B is the major mucin in the gel phase of sputum in chronic obstructive pulmonary disease
    Sara Kirkham
    1Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Am J Respir Crit Care Med 178:1033-9. 2008
  9. pmc Muc5ac: a critical component mediating the rejection of enteric nematodes
    Sumaira Z Hasnain
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, England, UK
    J Exp Med 208:893-900. 2011
  10. pmc Targeted induction of endoplasmic reticulum stress induces cartilage pathology
    M Helen Rajpar
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom
    PLoS Genet 5:e1000691. 2009

Collaborators

Detail Information

Publications27

  1. ncbi request reprint Structure and function of the polymeric mucins in airways mucus
    David J Thornton
    Wellcome Trust Center for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    Annu Rev Physiol 70:459-86. 2008
    ..g., asthma, cystic fibrosis, and chronic obstructive pulmonary disease). The roles of these enormous, multifunctional, O-linked glycoproteins in health and disease are discussed...
  2. pmc Reduced cell proliferation and increased apoptosis are significant pathological mechanisms in a murine model of mild pseudoachondroplasia resulting from a mutation in the C-terminal domain of COMP
    Katarzyna A Piróg-Garcia
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, UK
    Hum Mol Genet 16:2072-88. 2007
    ..This ultimately affects the morphology and proliferation of growth plate chondrocytes, eventually leading to chondrodysplasia and reduced long bone growth...
  3. ncbi request reprint Calcium-dependent protein interactions in MUC5B provide reversible cross-links in salivary mucus
    Bertrand D E Raynal
    Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, 2 205 Stopford Bldg, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 278:28703-10. 2003
    ..The results suggested that oligomeric MUC5B in saliva is assembled into much larger linear or branched assemblies through calcium-mediated protein cross-links...
  4. pmc An unfolded protein response is the initial cellular response to the expression of mutant matrilin-3 in a mouse model of multiple epiphyseal dysplasia
    Seema Nundlall
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK
    Cell Stress Chaperones 15:835-49. 2010
    ..We performed similar studies with a mouse model of Schmid metaphyseal chondrodysplasia, but again this treatment did not improve the phenotype...
  5. pmc Structural and functional characterization of recombinant matrilin-3 A-domain and implications for human genetic bone diseases
    Maryline Fresquet
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 282:34634-43. 2007
    ..Furthermore, we have also determined that the matrilin-3 A-domain appears to bind exclusively to the COL3 domain of type IX collagen and that this binding is abolished in the presence of a disease causing mutation in type IX collagen...
  6. doi request reprint Proteomic analysis of polymeric salivary mucins: no evidence for MUC19 in human saliva
    Karine Rousseau
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Manchester M13 9PT, UK
    Biochem J 413:545-52. 2008
    ..Our results suggest that unlike human saliva, which contains MUC5B, cow, horse and rat saliva are a heterogeneous mixture of Muc5b and Muc19. The functional consequence of these species differences remains to be elucidated...
  7. pmc Muc5b is the major polymeric mucin in mucus from thoroughbred horses with and without airway mucus accumulation
    Karine Rousseau
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    PLoS ONE 6:e19678. 2011
    ....
  8. pmc MUC5B is the major mucin in the gel phase of sputum in chronic obstructive pulmonary disease
    Sara Kirkham
    1Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Am J Respir Crit Care Med 178:1033-9. 2008
    ..Therefore, we hypothesized that the polymeric mucin composition or properties may be different in the sputum from individuals with COPD and smokers without airflow obstruction...
  9. pmc Muc5ac: a critical component mediating the rejection of enteric nematodes
    Sumaira Z Hasnain
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, England, UK
    J Exp Med 208:893-900. 2011
    ..Thus, for the first time, we identify a single mucin, Muc5ac, as a direct and critical mediator of resistance during intestinal nematode infection...
  10. pmc Targeted induction of endoplasmic reticulum stress induces cartilage pathology
    M Helen Rajpar
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom
    PLoS Genet 5:e1000691. 2009
    ..These findings support the contention that ER stress may play a direct role in the pathogenesis of many connective tissue disorders associated with the expression of mutant extracellular matrix proteins...
  11. ncbi request reprint Muc5b and Muc5ac are the major oligomeric mucins in equine airway mucus
    Karine Rousseau
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Am J Physiol Lung Cell Mol Physiol 292:L1396-404. 2007
    ..This study will form the basis for future studies to analyze the contribution of these two mucins to equine airways pathology associated with mucus accumulation...
  12. doi request reprint Desulfurization of mucin by Pseudomonas aeruginosa: influence of sulfate in the lungs of cystic fibrosis patients
    Camilla V Robinson
    Faculty of Life Sciences, University of Manchester, Oxford Rd, Manchester M13 9PT, UK
    J Med Microbiol 61:1644-53. 2012
    ..aeruginosa and the airways of CF patients...
  13. ncbi request reprint From mucins to mucus: toward a more coherent understanding of this essential barrier
    David J Thornton
    Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Manchester M13 9PT, UK
    Proc Am Thorac Soc 1:54-61. 2004
    ..Such studies will provide a clearer perception of airways mucosal protection and may highlight specific components as potential targets for therapeutic strategies for the treatment of hypersecretory disease...
  14. ncbi request reprint Identification of molecular intermediates in the assembly pathway of the MUC5AC mucin
    John K Sheehan
    School of Biological Sciences, University of Manchester, Michael Smith Building, Manchester M13 9PT, United Kingdom
    J Biol Chem 279:15698-705. 2004
    ..These data indicate that this oligomeric mucin follows a similar assembly to the von Willebrand factor glycoprotein to yield long linear disulfide-linked chains...
  15. doi request reprint A novel role for Gtb1p in glucose trimming of N-linked glycans
    Robert P Quinn
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Glycobiology 19:1408-16. 2009
    ..Our data demonstrate that both the MRH and G2B domains of Gtb1p contribute to the Glc2 trimming event but that the MRH domain is essential for Glc1 trimming...
  16. pmc Serine protease(s) secreted by the nematode Trichuris muris degrade the mucus barrier
    Sumaira Z Hasnain
    Immunity, Infection and Inflammation, Mater Medical Research Institute, Brisbane, Australia
    PLoS Negl Trop Dis 6:e1856. 2012
    ..However, during a T(H)2-mediated worm expulsion response, serpins, Muc5ac and increased levels of Muc2 protect the barrier from degradation by the nematode secreted protease(s)...
  17. pmc Concentrated solutions of salivary MUC5B mucin do not replicate the gel-forming properties of saliva
    Bertrand D E Raynal
    Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Manchester M13 9PT, UK
    Biochem J 362:289-96. 2002
    ....
  18. pmc Expression and secretion of Aspergillus fumigatus proteases are regulated in response to different protein substrates
    Edward Farnell
    Respiratory Research Group, School of Translational Medicine, University of Manchester, Manchester M13 9PT, UK
    Fungal Biol 116:1003-12. 2012
    ..There is a requirement for the standardisation of A. fumigatus allergen extracts used both in clinical diagnosis of A. fumigatus allergy and in research studies...
  19. pmc Mucin gene deficiency in mice impairs host resistance to an enteric parasitic infection
    Sumaira Z Hasnain
    Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Gastroenterology 138:1763-71. 2010
    ..Nevertheless, the precise role of mucins in host defense in nematode infection is not known. We investigated the role of the mucin (Muc2) in worm expulsion and host immunity in a model of nematode infection...
  20. doi request reprint Detecting, visualising, and quantifying mucins
    Ceri A Harrop
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, UK
    Methods Mol Biol 842:49-66. 2012
    ..Notes along with each section advice the researcher on best practice and describe any associated limitations of a particular technique from which the researcher can further develop a particular protocol...
  21. pmc Heterogeneity of airways mucus: variations in the amounts and glycoforms of the major oligomeric mucins MUC5AC and MUC5B
    Sara Kirkham
    Wellcome Trust Centre for Cell Matrix Research, University of Manchester, School of Biological Sciences, 2 205, Stopford Building, Manchester M13 9PT, UK
    Biochem J 361:537-46. 2002
    ..Finally, we show that the amounts and glycosylated variants of the MUC5AC and MUC5B mucins can be altered significantly in diseased airways with, for instance, an increase in the low-charge form of the MUC5B mucin in CF and COPD mucus...
  22. pmc Decreased chondrocyte proliferation and dysregulated apoptosis in the cartilage growth plate are key features of a murine model of epiphyseal dysplasia caused by a matn3 mutation
    Matthew P Leighton
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
    Hum Mol Genet 16:1728-41. 2007
    ....
  23. pmc The alternatively spliced type III connecting segment of fibronectin is a zinc-binding module
    Janet A Askari
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Matrix Biol 26:485-93. 2007
    ..Taken together, these data demonstrate that the IIICS region of FN is a novel zinc-binding module...
  24. ncbi request reprint A reproducible protocol for analysis of the proteome of Trypanosoma brucei by 2-dimensional gel electrophoresis
    Frederick J van Deursen
    School of Biological Sciences, University of Manchester, 2 14 Stopford Building, Manchester M13 9PT, UK
    Mol Biochem Parasitol 128:107-10. 2003
  25. ncbi request reprint Partial characterisation of high-molecular weight glycoconjugates in the trail mucus of the freshwater pond snail Lymnaea stagnalis
    Simon Ballance
    School of Biological Sciences, University of Manchester, Manchester M13 9PL, UK
    Comp Biochem Physiol B Biochem Mol Biol 137:475-86. 2004
    ....
  26. ncbi request reprint MUC16 is produced in tracheal surface epithelium and submucosal glands and is present in secretions from normal human airway and cultured bronchial epithelial cells
    Julia R Davies
    Mucosal Biology Group, Department of Experimental Medical Science, Lund University, BMC, C13, S 221 84 Lund, Sweden
    Int J Biochem Cell Biol 39:1943-54. 2007
    ....
  27. pmc Aberrant mucin assembly in mice causes endoplasmic reticulum stress and spontaneous inflammation resembling ulcerative colitis
    Chad K Heazlewood
    Mucin and IBD Research Teams, Mucosal Diseases Program, Mater Medical Research Institute, and The University of Queensland, Aubigny Place, Mater Health Services, South Brisbane, Queensland, Australia
    PLoS Med 5:e54. 2008
    ..In this study we used random mutagenesis to produce two murine models of inflammatory bowel disease, characterised the basis and nature of the inflammation in these mice, and compared the pathology with human ulcerative colitis...