Genomes and Genes
Christopher R Thornton
Affiliation: University of Exeter
- Production of a monoclonal antibody specific to the genus Trichoderma and closely related fungi, and its use to detect Trichoderma spp. in naturally infested compostsChristopher R Thornton
Washington Singer Laboratories, School of Biological Sciences, University of Exeter, Perry Road, Exeter, Devon EX4 4QG, UK
Microbiology 148:1263-79. 2002..in naturally infested composts. The specificity of this technique was confirmed by phylogenetic analysis based on sequences of the ITS1-5.8S-ITS2 rRNA-encoding regions of the isolates...
- A P-type ATPase required for rice blast disease and induction of host resistanceMartin J Gilbert
School of Biosciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter EX4 4QG, UK
Nature 440:535-9. 2006....
- Immunofluorescence microscopy and immunogold EM for investigating fungal infection of plantsChristopher R Thornton
School of Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
Nat Protoc 1:2506-11. 2006..Results from the IF and IES procedures can be obtained within 4-5 h. Sample preparation for IEM takes approximately 4 days. Gold labeling and visualization of samples can be completed within 4 h...
- Detection of the sour-rot pathogen Geotrichum candidum in tomato fruit and juice by using a highly specific monoclonal antibody-based ELISAChristopher R Thornton
University of Exeter, Exeter, United Kingdom
Int J Food Microbiol 143:166-72. 2010..Specificity of the ELISA was confirmed by sequencing of the internally transcribed spacer (ITS) 1-5.8S-ITS2 rRNA-encoding regions of fungi isolated from naturally-infected tomatoes...
- Tracking the emerging human pathogen Pseudallescheria boydii by using highly specific monoclonal antibodiesChristopher R Thornton
Hybridoma Laboratory, School of Biosciences, Geoffrey Pope Building, University of Exeter, Exeter, Devon, United Kingdom
Clin Vaccine Immunol 16:756-64. 2009..Specificity of the DAS-ELISA was confirmed by sequencing of the internally transcribed spacer 1 (ITS1)-5.8S-ITS2 rRNA-encoding regions of environmental isolates...
- Development of an immunochromatographic lateral-flow device for rapid serodiagnosis of invasive aspergillosisChristopher R Thornton
Hybridoma Laboratory, School of Biosciences, Geoffrey Pope Building, University of Exeter, Stocker Road, Exeter, Devon EX4 4QD, United Kingdom
Clin Vaccine Immunol 15:1095-105. 2008..The ease of use of the LFD provides a diagnostic platform for the routine testing of vulnerable patients who have an elevated risk of IA...
- Use of monoclonal antibodies to quantify the dynamics of alpha-galactosidase and endo-1,4-beta-glucanase production by Trichoderma hamatum during saprotrophic growth and sporulation in peatChristopher R Thornton
Fungal Biology Laboratory, Washington Singer Laboratories, School of Biological and Chemical Sciences, University of Exeter, Perry Road, Exeter, Devon EX4 4QG, UK
Environ Microbiol 7:737-49. 2005..It is postulated that release of oligosaccharides from polymeric hemicellulose by the constitutive spore-bound enzyme leads to AGL induction and could act as an environmental cue for spore germination...
- An immunological approach to quantifying the saprotrophic growth dynamics of Trichoderma species during antagonistic interactions with Rhizoctonia solani in a soil-less mixChristopher R Thornton
Fungal Biology Laboratory, Washington Singer Building, School of Biological and Chemical Sciences, University of Exeter, Perry Road, Exeter, Devon, EX4 4QG, England, UK
Environ Microbiol 6:323-34. 2004..asperellum. The assay was highly specific and only detected T. asperellum population dynamics. No cross-reactivity was found with extracts from soil samples containing contaminant fungi...
- Saprotrophic competitiveness and biocontrol fitness of a genetically modified strain of the plant-growth-promoting fungus Trichoderma hamatum GD12Lauren S Ryder
Biosciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
Microbiology 158:84-97. 2012..Furthermore, it highlights the need for a holistic approach to the development of genetically modified Trichoderma strains for use as crop stimulants and biocontrol agents in plant agriculture...
- Four conserved intramolecular disulphide linkages are required for secretion and cell wall localization of a hydrophobin during fungal morphogenesisMichael J Kershaw
School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter EX4 4QG, UK
Mol Microbiol 56:117-25. 2005..This provides the first genetic evidence that disulphide bridges in a hydrophobin are dispensable for aggregation, but essential for secretion...
- A fungal metallothionein is required for pathogenicity of Magnaporthe griseaSara L Tucker
School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Exeter EX4 4QG, United Kingdom
Plant Cell 16:1575-88. 2004..Mmt1 appears to be distributed in the inner side of the cell wall of the fungus. These findings indicate that Mmt1-like metallothioneins may play a novel role in fungal cell wall biochemistry that is required for fungal virulence...
- Phylogenomic analysis demonstrates a pattern of rare and ancient horizontal gene transfer between plants and fungiThomas A Richards
Centre for Eukaryotic Evolutionary Microbiology, School of Biosciences, University of Exeter, Exeter, EX4 4QD, United Kingdom
Plant Cell 21:1897-911. 2009..Our study suggests that genetic exchange between plants and fungi is exceedingly rare, particularly among the angiosperms, but has occurred during their evolutionary history and added important metabolic traits to plant lineages...
- Detection of invasive aspergillosisChristopher R Thornton
Hybridoma Laboratory, School of Biosciences, University of Exeter, Exeter, United Kingdom
Adv Appl Microbiol 70:187-216. 2010....
- Evolution of filamentous plant pathogens: gene exchange across eukaryotic kingdomsThomas A Richards
School of Biosciences, University of Exeter, Geoffrey Pope Building, Exeter EX4 4QD, United Kingdom
Curr Biol 16:1857-64. 2006....
- NADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungusLauren S Ryder
School of Biosciences, University of Exeter, Exeter EX4 4QD, United Kingdom
Proc Natl Acad Sci U S A 110:3179-84. 2013....
- Production of monoclonal antibodies to plant pathogensChristopher R Thornton
Washington Singer Laboratories, University of Exeter, UK
Methods Mol Biol 508:63-74. 2009..Hybridoma technology allows the limitless production of highly specific antibodies which can be used to identify pathogens to the species or even sub-species level...
- The glyoxylate cycle is required for temporal regulation of virulence by the plant pathogenic fungus Magnaporthe griseaZheng-Yi Wang
School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Exeter EX4 4QG, UK
Mol Microbiol 47:1601-12. 2003..Our results indicate that the glyoxylate cycle is also important in this plant pathogenic fungus, demonstrating the widespread utility of the pathway in microbial pathogenesis...