Christopher R Thornton

Summary

Affiliation: University of Exeter
Country: UK

Publications

  1. ncbi Production of a monoclonal antibody specific to the genus Trichoderma and closely related fungi, and its use to detect Trichoderma spp. in naturally infested composts
    Christopher R Thornton
    Washington Singer Laboratories, School of Biological Sciences, University of Exeter, Perry Road, Exeter, Devon EX4 4QG, UK
    Microbiology 148:1263-79. 2002
  2. ncbi A P-type ATPase required for rice blast disease and induction of host resistance
    Martin J Gilbert
    School of Biosciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter EX4 4QG, UK
    Nature 440:535-9. 2006
  3. ncbi Immunofluorescence microscopy and immunogold EM for investigating fungal infection of plants
    Christopher R Thornton
    School of Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
    Nat Protoc 1:2506-11. 2006
  4. ncbi Detection of the sour-rot pathogen Geotrichum candidum in tomato fruit and juice by using a highly specific monoclonal antibody-based ELISA
    Christopher R Thornton
    University of Exeter, Exeter, United Kingdom
    Int J Food Microbiol 143:166-72. 2010
  5. ncbi Tracking the emerging human pathogen Pseudallescheria boydii by using highly specific monoclonal antibodies
    Christopher R Thornton
    Hybridoma Laboratory, School of Biosciences, Geoffrey Pope Building, University of Exeter, Exeter, Devon, United Kingdom
    Clin Vaccine Immunol 16:756-64. 2009
  6. ncbi Development of an immunochromatographic lateral-flow device for rapid serodiagnosis of invasive aspergillosis
    Christopher R Thornton
    Hybridoma Laboratory, School of Biosciences, Geoffrey Pope Building, University of Exeter, Stocker Road, Exeter, Devon EX4 4QD, United Kingdom
    Clin Vaccine Immunol 15:1095-105. 2008
  7. ncbi Use of monoclonal antibodies to quantify the dynamics of alpha-galactosidase and endo-1,4-beta-glucanase production by Trichoderma hamatum during saprotrophic growth and sporulation in peat
    Christopher R Thornton
    Fungal Biology Laboratory, Washington Singer Laboratories, School of Biological and Chemical Sciences, University of Exeter, Perry Road, Exeter, Devon EX4 4QG, UK
    Environ Microbiol 7:737-49. 2005
  8. ncbi An immunological approach to quantifying the saprotrophic growth dynamics of Trichoderma species during antagonistic interactions with Rhizoctonia solani in a soil-less mix
    Christopher R Thornton
    Fungal Biology Laboratory, Washington Singer Building, School of Biological and Chemical Sciences, University of Exeter, Perry Road, Exeter, Devon, EX4 4QG, England, UK
    Environ Microbiol 6:323-34. 2004
  9. ncbi Saprotrophic competitiveness and biocontrol fitness of a genetically modified strain of the plant-growth-promoting fungus Trichoderma hamatum GD12
    Lauren S Ryder
    Biosciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
    Microbiology 158:84-97. 2012
  10. ncbi Four conserved intramolecular disulphide linkages are required for secretion and cell wall localization of a hydrophobin during fungal morphogenesis
    Michael J Kershaw
    School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter EX4 4QG, UK
    Mol Microbiol 56:117-25. 2005

Collaborators

  • Nicholas J Talbot
  • Thomas A Richards
  • Claus Jacob
  • Joel B Dacks
  • Lauren S Ryder
  • Michael J Kershaw
  • Martin J Gilbert
  • Gavin E Wakley
  • Sara L Tucker
  • Zheng-Yi Wang
  • Jisheng Chen
  • Zonghua Wang
  • Yasin F Dagdas
  • Thomas A Mentlak
  • Martin Schuster
  • Darren M Soanes
  • Beverley D Harris
  • Karen Tasker
  • Greg Giles
  • Martin Egan
  • Li Debao

Detail Information

Publications17

  1. ncbi Production of a monoclonal antibody specific to the genus Trichoderma and closely related fungi, and its use to detect Trichoderma spp. in naturally infested composts
    Christopher R Thornton
    Washington Singer Laboratories, School of Biological Sciences, University of Exeter, Perry Road, Exeter, Devon EX4 4QG, UK
    Microbiology 148:1263-79. 2002
    ..in naturally infested composts. The specificity of this technique was confirmed by phylogenetic analysis based on sequences of the ITS1-5.8S-ITS2 rRNA-encoding regions of the isolates...
  2. ncbi A P-type ATPase required for rice blast disease and induction of host resistance
    Martin J Gilbert
    School of Biosciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter EX4 4QG, UK
    Nature 440:535-9. 2006
    ....
  3. ncbi Immunofluorescence microscopy and immunogold EM for investigating fungal infection of plants
    Christopher R Thornton
    School of Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
    Nat Protoc 1:2506-11. 2006
    ..Results from the IF and IES procedures can be obtained within 4-5 h. Sample preparation for IEM takes approximately 4 days. Gold labeling and visualization of samples can be completed within 4 h...
  4. ncbi Detection of the sour-rot pathogen Geotrichum candidum in tomato fruit and juice by using a highly specific monoclonal antibody-based ELISA
    Christopher R Thornton
    University of Exeter, Exeter, United Kingdom
    Int J Food Microbiol 143:166-72. 2010
    ..Specificity of the ELISA was confirmed by sequencing of the internally transcribed spacer (ITS) 1-5.8S-ITS2 rRNA-encoding regions of fungi isolated from naturally-infected tomatoes...
  5. ncbi Tracking the emerging human pathogen Pseudallescheria boydii by using highly specific monoclonal antibodies
    Christopher R Thornton
    Hybridoma Laboratory, School of Biosciences, Geoffrey Pope Building, University of Exeter, Exeter, Devon, United Kingdom
    Clin Vaccine Immunol 16:756-64. 2009
    ..Specificity of the DAS-ELISA was confirmed by sequencing of the internally transcribed spacer 1 (ITS1)-5.8S-ITS2 rRNA-encoding regions of environmental isolates...
  6. ncbi Development of an immunochromatographic lateral-flow device for rapid serodiagnosis of invasive aspergillosis
    Christopher R Thornton
    Hybridoma Laboratory, School of Biosciences, Geoffrey Pope Building, University of Exeter, Stocker Road, Exeter, Devon EX4 4QD, United Kingdom
    Clin Vaccine Immunol 15:1095-105. 2008
    ..The ease of use of the LFD provides a diagnostic platform for the routine testing of vulnerable patients who have an elevated risk of IA...
  7. ncbi Use of monoclonal antibodies to quantify the dynamics of alpha-galactosidase and endo-1,4-beta-glucanase production by Trichoderma hamatum during saprotrophic growth and sporulation in peat
    Christopher R Thornton
    Fungal Biology Laboratory, Washington Singer Laboratories, School of Biological and Chemical Sciences, University of Exeter, Perry Road, Exeter, Devon EX4 4QG, UK
    Environ Microbiol 7:737-49. 2005
    ..It is postulated that release of oligosaccharides from polymeric hemicellulose by the constitutive spore-bound enzyme leads to AGL induction and could act as an environmental cue for spore germination...
  8. ncbi An immunological approach to quantifying the saprotrophic growth dynamics of Trichoderma species during antagonistic interactions with Rhizoctonia solani in a soil-less mix
    Christopher R Thornton
    Fungal Biology Laboratory, Washington Singer Building, School of Biological and Chemical Sciences, University of Exeter, Perry Road, Exeter, Devon, EX4 4QG, England, UK
    Environ Microbiol 6:323-34. 2004
    ..asperellum. The assay was highly specific and only detected T. asperellum population dynamics. No cross-reactivity was found with extracts from soil samples containing contaminant fungi...
  9. ncbi Saprotrophic competitiveness and biocontrol fitness of a genetically modified strain of the plant-growth-promoting fungus Trichoderma hamatum GD12
    Lauren S Ryder
    Biosciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
    Microbiology 158:84-97. 2012
    ..Furthermore, it highlights the need for a holistic approach to the development of genetically modified Trichoderma strains for use as crop stimulants and biocontrol agents in plant agriculture...
  10. ncbi Four conserved intramolecular disulphide linkages are required for secretion and cell wall localization of a hydrophobin during fungal morphogenesis
    Michael J Kershaw
    School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter EX4 4QG, UK
    Mol Microbiol 56:117-25. 2005
    ..This provides the first genetic evidence that disulphide bridges in a hydrophobin are dispensable for aggregation, but essential for secretion...
  11. ncbi A fungal metallothionein is required for pathogenicity of Magnaporthe grisea
    Sara L Tucker
    School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Exeter EX4 4QG, United Kingdom
    Plant Cell 16:1575-88. 2004
    ..Mmt1 appears to be distributed in the inner side of the cell wall of the fungus. These findings indicate that Mmt1-like metallothioneins may play a novel role in fungal cell wall biochemistry that is required for fungal virulence...
  12. ncbi Phylogenomic analysis demonstrates a pattern of rare and ancient horizontal gene transfer between plants and fungi
    Thomas A Richards
    Centre for Eukaryotic Evolutionary Microbiology, School of Biosciences, University of Exeter, Exeter, EX4 4QD, United Kingdom
    Plant Cell 21:1897-911. 2009
    ..Our study suggests that genetic exchange between plants and fungi is exceedingly rare, particularly among the angiosperms, but has occurred during their evolutionary history and added important metabolic traits to plant lineages...
  13. ncbi Detection of invasive aspergillosis
    Christopher R Thornton
    Hybridoma Laboratory, School of Biosciences, University of Exeter, Exeter, United Kingdom
    Adv Appl Microbiol 70:187-216. 2010
    ....
  14. ncbi Evolution of filamentous plant pathogens: gene exchange across eukaryotic kingdoms
    Thomas A Richards
    School of Biosciences, University of Exeter, Geoffrey Pope Building, Exeter EX4 4QD, United Kingdom
    Curr Biol 16:1857-64. 2006
    ....
  15. ncbi NADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungus
    Lauren S Ryder
    School of Biosciences, University of Exeter, Exeter EX4 4QD, United Kingdom
    Proc Natl Acad Sci U S A 110:3179-84. 2013
    ....
  16. ncbi Production of monoclonal antibodies to plant pathogens
    Christopher R Thornton
    Washington Singer Laboratories, University of Exeter, UK
    Methods Mol Biol 508:63-74. 2009
    ..Hybridoma technology allows the limitless production of highly specific antibodies which can be used to identify pathogens to the species or even sub-species level...
  17. ncbi The glyoxylate cycle is required for temporal regulation of virulence by the plant pathogenic fungus Magnaporthe grisea
    Zheng-Yi Wang
    School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Exeter EX4 4QG, UK
    Mol Microbiol 47:1601-12. 2003
    ..Our results indicate that the glyoxylate cycle is also important in this plant pathogenic fungus, demonstrating the widespread utility of the pathway in microbial pathogenesis...