P J Thornalley

Summary

Affiliation: University of Essex
Country: UK

Publications

  1. ncbi request reprint Isothiocyanates: mechanism of cancer chemopreventive action
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, Essex, UK
    Anticancer Drugs 13:331-8. 2002
  2. pmc Quantitative screening of advanced glycation endproducts in cellular and extracellular proteins by tandem mass spectrometry
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester CO4 3SQ, Essex, UK
    Biochem J 375:581-92. 2003
  3. ncbi request reprint Use of aminoguanidine (Pimagedine) to prevent the formation of advanced glycation endproducts
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, CO4 3SQ, Essex, UK
    Arch Biochem Biophys 419:31-40. 2003
  4. ncbi request reprint The enzymatic defence against glycation in health, disease and therapeutics: a symposium to examine the concept
    P J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem Soc Trans 31:1341-2. 2003
  5. ncbi request reprint Glyoxalase I--structure, function and a critical role in the enzymatic defence against glycation
    P J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem Soc Trans 31:1343-8. 2003
  6. ncbi request reprint Protecting the genome: defence against nucleotide glycation and emerging role of glyoxalase I overexpression in multidrug resistance in cancer chemotherapy
    P J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem Soc Trans 31:1372-7. 2003
  7. ncbi request reprint Advanced glycation end products in renal failure
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, UK
    J Ren Nutr 16:178-84. 2006
  8. ncbi request reprint Unease on the role of glyoxalase 1 in high-anxiety-related behaviour
    Paul J Thornalley
    Disease Mechanisms and Therapeutics Research Group, Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3Q4, UK
    Trends Mol Med 12:195-9. 2006
  9. ncbi request reprint Measurement of protein glycation, glycated peptides, and glycation free adducts
    Paul J Thornalley
    Disease Mechanisms and Therapeutics Research Group, Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, United Kingdom
    Perit Dial Int 25:522-33. 2005
  10. ncbi request reprint Peptide mapping of human serum albumin modified minimally by methylglyoxal in vitro and in vivo
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK
    Ann N Y Acad Sci 1043:260-6. 2005

Collaborators

Detail Information

Publications64

  1. ncbi request reprint Isothiocyanates: mechanism of cancer chemopreventive action
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, Essex, UK
    Anticancer Drugs 13:331-8. 2002
    ..These features of isothiocyanate metabolism and chemoprevention deserve further investigation...
  2. pmc Quantitative screening of advanced glycation endproducts in cellular and extracellular proteins by tandem mass spectrometry
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester CO4 3SQ, Essex, UK
    Biochem J 375:581-92. 2003
    ..Increased levels of these advanced glycation endproducts were associated with vascular complications in diabetes and uraemia...
  3. ncbi request reprint Use of aminoguanidine (Pimagedine) to prevent the formation of advanced glycation endproducts
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, CO4 3SQ, Essex, UK
    Arch Biochem Biophys 419:31-40. 2003
    ..Pharmacological scavenging of alpha-oxoaldehydes or stimulation of host alpha-oxoaldehyde detoxification remains a worthy therapeutic strategy to prevent diabetic complications and other AGE-related disorders...
  4. ncbi request reprint The enzymatic defence against glycation in health, disease and therapeutics: a symposium to examine the concept
    P J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem Soc Trans 31:1341-2. 2003
    ..Improved understanding of the balance between glycation and the enzymatic anti-glycation defence will advance disease diagnosis and therapy...
  5. ncbi request reprint Glyoxalase I--structure, function and a critical role in the enzymatic defence against glycation
    P J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem Soc Trans 31:1343-8. 2003
    ..Glyoxalase I has a critical role in the prevention of glycation reactions mediated by methylglyoxal, glyoxal and other alpha-oxoaldehydes in vivo...
  6. ncbi request reprint Protecting the genome: defence against nucleotide glycation and emerging role of glyoxalase I overexpression in multidrug resistance in cancer chemotherapy
    P J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem Soc Trans 31:1372-7. 2003
    ..It countered drug resistance and was a potent antitumour agent against lung and prostate carcinoma. Glyoxalase I overexpression was also found in invasive ovarian cancer and breast cancer...
  7. ncbi request reprint Advanced glycation end products in renal failure
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, UK
    J Ren Nutr 16:178-84. 2006
    ..Their accumulation may impair vascular cell function and contribute to morbidity and mortality in renal disease...
  8. ncbi request reprint Unease on the role of glyoxalase 1 in high-anxiety-related behaviour
    Paul J Thornalley
    Disease Mechanisms and Therapeutics Research Group, Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3Q4, UK
    Trends Mol Med 12:195-9. 2006
    ....
  9. ncbi request reprint Measurement of protein glycation, glycated peptides, and glycation free adducts
    Paul J Thornalley
    Disease Mechanisms and Therapeutics Research Group, Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, United Kingdom
    Perit Dial Int 25:522-33. 2005
    ..Glycation free adducts are the major form of glycation adduct eliminated in dialysate. LC-MS/MS may now be used to quantify concentrations, extents of protein modification, clearances, and excretion rates of glycation adducts in uremia...
  10. ncbi request reprint Peptide mapping of human serum albumin modified minimally by methylglyoxal in vitro and in vivo
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK
    Ann N Y Acad Sci 1043:260-6. 2005
    ..Modification of arginine residues by methylglyoxal may be particularly damaging because arginine residues have a high frequency of occurrence in ligand and substrate recognition sites in receptor and enzyme active sites...
  11. ncbi request reprint Dicarbonyl intermediates in the maillard reaction
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK
    Ann N Y Acad Sci 1043:111-7. 2005
    ..Along with fructosamine formation, alpha-oxoaldehyde intermediates of the Maillard reaction represent a major source of damage to the proteome and genome...
  12. ncbi request reprint The potential role of thiamine (vitamin B1) in diabetic complications
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
    Curr Diabetes Rev 1:287-98. 2005
    ....
  13. ncbi request reprint Mass spectrometric monitoring of albumin in uremia
    P J Thornalley
    Department of Biological Sciences, University of Essex, Essex, England, United Kingdom Kingdom
    Kidney Int 58:2228-34. 2000
    ..AGE-modified peptides are thought to bind and modify plasma proteins. Monitoring of the consequent increase in molecular mass of serum albumin may be used in surveillance of the clinical management of uremia...
  14. ncbi request reprint Glutathione-dependent detoxification of alpha-oxoaldehydes by the glyoxalase system: involvement in disease mechanisms and antiproliferative activity of glyoxalase I inhibitors
    P J Thornalley
    Department of Biological and Chemical Sciences, University of Essex, Colchester, UK
    Chem Biol Interact 111:137-51. 1998
    ..The modification of DNA induces mutagenesis and apoptosis. The modification of proteins leads to protein degradation and activation of a cytokine-mediated immune response...
  15. ncbi request reprint Suppression of the accumulation of triosephosphates and increased formation of methylglyoxal in human red blood cells during hyperglycaemia by thiamine in vitro
    P J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester CO4 3SQ, Essex, UK
    J Biochem 129:543-9. 2001
    ..Biochemical changes implicated in the development of diabetic complications were thereby prevented. This provides a biochemical basis for high dose thiamine therapy for the prevention of diabetic complications...
  16. pmc Formation of glyoxal, methylglyoxal and 3-deoxyglucosone in the glycation of proteins by glucose
    P J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester CO43SQ, Essex, U K
    Biochem J 344:109-16. 1999
    ..Short periods of hyperglycaemia, as occur in impaired glucose tolerance, may be sufficient to increase the concentrations of alpha-oxoaldehydes in vivo...
  17. ncbi request reprint Kinetics and mechanism of the reaction of aminoguanidine with the alpha-oxoaldehydes glyoxal, methylglyoxal, and 3-deoxyglucosone under physiological conditions
    P J Thornalley
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, United Kingdom
    Biochem Pharmacol 60:55-65. 2000
    ..This effect is limited, however, by the rapid renal elimination of AG. Decreased AGE formation is implicated in the prevention of microvascular complications of diabetes by AG...
  18. doi request reprint Increased protein damage in renal glomeruli, retina, nerve, plasma and urine and its prevention by thiamine and benfotiamine therapy in a rat model of diabetes
    N Karachalias
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex, UK
    Diabetologia 53:1506-16. 2010
    ..The aim of this study was to quantify protein damage by glycation, oxidation and nitration in a rat model of diabetes at the sites of development of microvascular complications, including the effects of thiamine and benfotiamine therapy...
  19. pmc High prevalence of low plasma thiamine concentration in diabetes linked to a marker of vascular disease
    P J Thornalley
    Department of Biological Sciences, University of Essex, Colchester, Essex, UK
    Diabetologia 50:2164-70. 2007
    ..To assess thiamine status by analysis of plasma, erythrocytes and urine in type 1 and type 2 diabetic patients and links to markers of vascular dysfunction...
  20. ncbi request reprint Advanced glycation endproducts: what is their relevance to diabetic complications?
    N Ahmed
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex, CO4 3SQ, UK
    Diabetes Obes Metab 9:233-45. 2007
    ..Increasing expression of enzymes of the enzymatic defence against glycation provides a novel and potentially effective future therapeutic strategy to suppress protein glycation...
  21. ncbi request reprint Trioses and related substances: tools for the study of pancreatic beta-cell function
    L Best
    Department of Medicine, University of Manchester, England, UK
    Biochem Pharmacol 57:583-8. 1999
    ..These substances are also likely to be of pathophysiological importance, especially in the context of sugar toxicity and autoxidative cell damage...
  22. ncbi request reprint Removal of advanced glycation end products in clinical renal failure by peritoneal dialysis and haemodialysis
    S Agalou
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem Soc Trans 31:1394-6. 2003
    ..Inadequate clearance of free AGEs may be linked to the increased risk of cardiovascular disease in patients with renal failure...
  23. ncbi request reprint Degradation products of proteins damaged by glycation, oxidation and nitration in clinical type 1 diabetes
    N Ahmed
    Department of Biological Sciences, University of Essex, Colchester, Essex CO4 3SQ, UK
    Diabetologia 48:1590-603. 2005
    ....
  24. pmc The glyoxalase system: new developments towards functional characterization of a metabolic pathway fundamental to biological life
    P J Thornalley
    Department of Chemistry and Biological Chemistry, University of Essex, Colchester, U K
    Biochem J 269:1-11. 1990
  25. ncbi request reprint Accumulation of fructosyl-lysine and advanced glycation end products in the kidney, retina and peripheral nerve of streptozotocin-induced diabetic rats
    N Karachalias
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem Soc Trans 31:1423-5. 2003
    ..High-dose therapy with thiamine and Benfotiamine suppressed the accumulation of AGEs, and is a novel approach to preventing the development of diabetic complications...
  26. ncbi request reprint High-dose thiamine therapy counters dyslipidaemia in streptozotocin-induced diabetic rats
    R Babaei-Jadidi
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ, UK
    Diabetologia 47:2235-46. 2004
    ..In this study, we assessed if decrease of flux through the hexosamine pathway induced by high-dose thiamine therapy counters diabetic dyslipidaemia...
  27. pmc Signal transduction activated by the cancer chemopreventive isothiocyanates: cleavage of BID protein, tyrosine phosphorylation and activation of JNK
    K Xu
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex, CO4 3SQ, UK
    Br J Cancer 84:670-3. 2001
    ..This suggests that in isothiocyanate-induced apoptosis, the caspase pathway has an essential role, the JNK pathway a supporting role, and inhibition of protein tyrosine phosphatases is not involved...
  28. ncbi request reprint Quantitative screening of protein biomarkers of early glycation, advanced glycation, oxidation and nitrosation in cellular and extracellular proteins by tandem mass spectrometry multiple reaction monitoring
    N Ahmed
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem Soc Trans 31:1417-22. 2003
    ..The enzymatic defences against glycation, antioxidants and proteasomal protein degradation inside cells are probable factors regulating biomarker levels of cellular protein...
  29. ncbi request reprint Cell activation by glycated proteins. AGE receptors, receptor recognition factors and functional classification of AGEs
    P J Thornalley
    Department of Biological Sciences, University of Essex, Colchester, UK
    Cell Mol Biol (Noisy-le-grand) 44:1013-23. 1998
    ....
  30. pmc Protein and nucleotide damage by glyoxal and methylglyoxal in physiological systems--role in ageing and disease
    Paul J Thornalley
    Protein Damage and Systems Biology Research Group, Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, University Hospital, Coventry, UK
    Drug Metabol Drug Interact 23:125-50. 2008
    ..Latest research suggests there are functional modifications of this process--implying a role in cell signalling, ageing and disease...
  31. ncbi request reprint Increased protein glycation in cirrhosis and therapeutic strategies to prevent it
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK
    Ann N Y Acad Sci 1043:718-24. 2005
    ..Therapeutic strategies to decrease dicarbonyl compounds may be beneficial, such as dicarbonyl scavengers, glutathione repleting agents, and high-dose thiamine therapy...
  32. ncbi request reprint High-dose thiamine therapy counters dyslipidemia and advanced glycation of plasma protein in streptozotocin-induced diabetic rats
    Nikolaos Karachalias
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK
    Ann N Y Acad Sci 1043:777-83. 2005
    ..These are probably lipid-derived aldehydes...
  33. ncbi request reprint High glucose increases angiopoietin-2 transcription in microvascular endothelial cells through methylglyoxal modification of mSin3A
    Dachun Yao
    Juvenile Diabetes Research Foundation International Center for Diabetic Complications Research, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 282:31038-45. 2007
    ..This novel mechanism for regulating gene expression may play a role in the pathobiology of diabetic vascular disease...
  34. ncbi request reprint Endogenous alpha-oxoaldehydes and formation of protein and nucleotide advanced glycation endproducts in tissue damage
    Paul J Thornalley
    Protein Damage and Systems Biology Research Group, Warwick Medical School and Systems Biology Centre, Clinical Sciences Research Institute, University of Warwick, University Hospital, Coventry CV2 2DX, UK
    Novartis Found Symp 285:229-43; discussion 243-6. 2007
    ..Tissue damage by alpha-oxoaldehyde glycation is implicated in diabetic and non-diabetic vascular disease, renal failure, cirrhosis, Alzheimer's disease, arthritis and ageing...
  35. ncbi request reprint Effect of storage, processing and cooking on glucosinolate content of Brassica vegetables
    Lijiang Song
    Disease Mechanisms and Therapeutic Research Group, Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
    Food Chem Toxicol 45:216-24. 2007
    ..Most of the loss of the glucosinolates (approximately 90%) was detected in the cooking water. Increased bioavailability of dietary isothiocyanates may be achieved by avoiding boiling of vegetables...
  36. pmc Preparation of nucleotide advanced glycation endproducts--imidazopurinone adducts formed by glycation of deoxyguanosine with glyoxal and methylglyoxal
    Thomas Fleming
    Department of Biological Sciences, University of Essex, Colchester, United Kingdom
    Ann N Y Acad Sci 1126:280-2. 2008
    ..Analysis of dG-G, dG-MG, and the oxidative marker 8-hydroxydeoxyguanosine in the DNA of cultured human cells and mononuclear leukocytes showed that nucleotide advanced glycation endproducts are major markers of DNA damage in human cells...
  37. ncbi request reprint Assay of advanced glycation endproducts in selected beverages and food by liquid chromatography with tandem mass spectrometric detection
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Colchester, UK
    Mol Nutr Food Res 49:691-9. 2005
    ..Experimental diabetes is associated with a marked increase in exposure to endogenous formation of methylglyoxal-derived hydroimidazolone which is linked to the development of diabetic nephropathy...
  38. ncbi request reprint Idiopathic hypoalbuminemia explained by reduced synthesis rate and an increased catabolic rate
    Berthil H C M T Prinsen
    Department of Vascular Medicine and Metabolism, University Medical Center, Utrecht, The Netherlands
    Clin Biochem 35:545-53. 2002
    ..To determine the contribution of albumin synthetic and catabolic rates to steady state levels in a patient with idiopathic hypoalbuminemia...
  39. ncbi request reprint Increased formation of methylglyoxal and protein glycation, oxidation and nitrosation in triosephosphate isomerase deficiency
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK
    Biochim Biophys Acta 1639:121-32. 2003
    ..The increased derangement of MG metabolism and associated glycation, oxidative and nitrosative stress in the propositus may be linked to neurodegenerative process in triosephosphate isomerase deficiency...
  40. ncbi request reprint Methods for studying the binding of advanced glycated proteins to receptors for advanced glycation endproducts (AGE receptors)
    Paul J Thornalley
    Department of Biological Sciences, Wivenhoe Park, University of Essex, Colchester, United Kingdom
    Methods Mol Biol 196:49-62. 2002
  41. ncbi request reprint Methylglyoxal-derived hydroimidazolone advanced glycation end-products of human lens proteins
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
    Invest Ophthalmol Vis Sci 44:5287-92. 2003
    ....
  42. ncbi request reprint Peptide mapping identifies hotspot site of modification in human serum albumin by methylglyoxal involved in ligand binding and esterase activity
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Colchester, Essex CO4 3SQ, UK
    J Biol Chem 280:5724-32. 2005
    ..Methylglyoxal modification of critical arginine residues, therefore, whether experimental or physiological, is expected to disrupt protein-ligand interactions and inactivate enzyme activity by hydroimidazolone formation...
  43. ncbi request reprint Transcription factor Nrf2 is essential for induction of NAD(P)H:quinone oxidoreductase 1, glutathione S-transferases, and glutamate cysteine ligase by broccoli seeds and isothiocyanates
    Gail K McWalter
    Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, United Kingdom
    J Nutr 134:3499S-3506S. 2004
    ..These experiments show that broccoli seeds are effective at inducing antioxidant and detoxication proteins, both in vivo and ex vivo, in an Nrf2-dependent manner...
  44. ncbi request reprint Processing of protein glycation, oxidation and nitrosation adducts in the liver and the effect of cirrhosis
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Central Campus, Colchester, Essex CO4 3SQ, UK
    J Hepatol 41:913-9. 2004
    ..We investigated the hepatic extraction of glycated, oxidised and nitrosated proteins in vivo...
  45. ncbi request reprint Nepsilon-(Carboxymethyl)lysine and 3-DG-imidazolone are major AGE structures in protein modification by 3-deoxyglucosone
    Tadashi Jono
    Department of Medical Biochemistry, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, 860 0811, Japan
    J Biochem 136:351-8. 2004
    ..Our results provide the first evidence that CML and 3-DG-imidazolone are major AGE structures in 3-DG-modified proteins, and that 3-DG-imidazolone provides a better marker for protein modification by 3-DG than pyrraline...
  46. ncbi request reprint Glycation in diabetic neuropathy: characteristics, consequences, causes, and therapeutic options
    Paul J Thornalley
    Department of Biological Sciences, University of Essex, Colchester, Essex CO4 3SQ, United Kingdom
    Int Rev Neurobiol 50:37-57. 2002
    ..Future possible therapeutic strategies are RAGE antagonists and inducers of the enzymatic antiglycation defense. More research is required to understand the role of glycation in the development of diabetic neuropathy...
  47. ncbi request reprint Profound mishandling of protein glycation degradation products in uremia and dialysis
    Stamatina Agalou
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
    J Am Soc Nephrol 16:1471-85. 2005
    ..It is concluded that there are severe deficits of protein glycation free adduct clearance in chronic renal failure and in ESRD on PD and HD therapy...
  48. pmc Chromatographic assay of glycation adducts in human serum albumin glycated in vitro by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and intrinsic fluorescence
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem J 364:15-24. 2002
    ..Significant proportions of arginine and lysine-derived AGEs in albumin modified highly by methylglyoxal, and lysine-derived AGEs in albumin modified highly by glucose, remain to be identified...
  49. ncbi request reprint Dietary AGEs and ALEs and risk to human health by their interaction with the receptor for advanced glycation endproducts (RAGE)--an introduction
    Paul J Thornalley
    Clinical Sciences Research Institute, University of Warwick, University Hospital, Coventry, UK
    Mol Nutr Food Res 51:1107-10. 2007
    ....
  50. ncbi request reprint Methylglyoxal administration induces diabetes-like microvascular changes and perturbs the healing process of cutaneous wounds
    Jorge Berlanga
    Centre for Genetic Engineering and Biotechnology, Ave 31 e 158 and 190 Playa 10600, Havana, Cuba
    Clin Sci (Lond) 109:83-95. 2005
    ..We conclude that exposure to increased MG in vivo is associated with the onset of microvascular damage and other diabetes-like complications within a normoglycaemic context...
  51. pmc Dicarbonyls linked to damage in the powerhouse: glycation of mitochondrial proteins and oxidative stress
    Naila Rabbani
    Protein Damage and Systems Biology Research Group, Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, University Hospital, Coventry CV2 2DX, UK
    Biochem Soc Trans 36:1045-50. 2008
    ..Future research will address the functional charges in mitochondrial proteins that are the targets for dicarbonyl glycation...
  52. pmc Assay of advanced glycation endproducts (AGEs): surveying AGEs by chromatographic assay with derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and application to Nepsilon-carboxymethyl-lysine- and Nepsilon-(1-carboxyethyl)lysine-modified a
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex CO4 3SQ, U K
    Biochem J 364:1-14. 2002
    ..The assay was applied to the characterization of human serum albumin minimally and highly modified by N(epsilon)-carboxymethyl-lysine and N(epsilon)-(1-carboxyethyl)-lysine...
  53. pmc Reversal of hyperglycemia-induced angiogenesis deficit of human endothelial cells by overexpression of glyoxalase 1 in vitro
    Usman Ahmed
    Department of Biological Sciences, University of Essex, Colchester, United Kingdom
    Ann N Y Acad Sci 1126:262-4. 2008
    ..We conclude that increased protection against dicarbonyl glycation of endothelial cell protein protects hyperglycemia-induced angiogenesis deficit...
  54. doi request reprint The dicarbonyl proteome: proteins susceptible to dicarbonyl glycation at functional sites in health, aging, and disease
    Naila Rabbani
    Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Coventry CV2 2DX, UK
    Ann N Y Acad Sci 1126:124-7. 2008
    ....
  55. doi request reprint Assay of 3-nitrotyrosine in tissues and body fluids by liquid chromatography with tandem mass spectrometric detection
    Naila Rabbani
    Protein Damage and Systems Biology Research Group, Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, University Hospital, Coventry, United Kingdom
    Methods Enzymol 440:337-59. 2008
    ..Changes of 3-nitrotyrosine residue and free 3-nitrotyrosine in diabetes, cirrhosis, acute and chronic renal failure, and neurological disorders, including Alzheimer's disease, are presented and compared with independent estimates...
  56. ncbi request reprint Prevention of incipient diabetic nephropathy by high-dose thiamine and benfotiamine
    Roya Babaei-Jadidi
    Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester, Essex, UK
    Diabetes 52:2110-20. 2003
    ..High-dose thiamine and benfotiamine therapy is a potential novel strategy for the prevention of clinical diabetic nephropathy...
  57. ncbi request reprint Stimulation of suicidal erythrocyte death by methylglyoxal
    Jan P Nicolay
    Department of Physiology, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 18:223-32. 2006
    ..In conclusion, methylglyoxal impairs energy production and anti-oxidative defense, effects contributing to the enhanced PS exposure of circulating erythrocytes and eventually resulting in anemia and deranged microcirculation...
  58. ncbi request reprint Methylglyoxal modification of mSin3A links glycolysis to angiopoietin-2 transcription
    Dachun Yao
    JDRF International Center for Diabetic Complications Research, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cell 124:275-86. 2006
    ....
  59. ncbi request reprint Glycation, receptor-mediated cell activation and vascular complications of diabetes
    Paul J Thornalley
    Diab Vasc Dis Res 1:21-2. 2004
  60. ncbi request reprint Analysis of glucosinolates, isothiocyanates, and amine degradation products in vegetable extracts and blood plasma by LC-MS/MS
    Lijiang Song
    Disease Mechanisms and Therapeutic Research Group, Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK
    Anal Biochem 347:234-43. 2005
    ..These methods provide facile access to comprehensive analytical data on the major dietary glucosinolates and related metabolites to quantify inputs and metabolic formation of these compounds in cancer prevention and related studies...
  61. ncbi request reprint Glycated and oxidized protein degradation products are indicators of fasting and postprandial hyperglycemia in diabetes
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Colchester, UK
    Diabetes Care 28:2465-71. 2005
    ....
  62. ncbi request reprint Glycation free adduct accumulation in renal disease: the new AGE
    Paul J Thornalley
    Pediatr Nephrol 20:1515-22. 2005
    ..AGE free adducts may also contribute to vascular disease in uraemia. They represent an important new age for glycation research in nephrology...
  63. ncbi request reprint Protein glycation, oxidation and nitration adduct residues and free adducts of cerebrospinal fluid in Alzheimer's disease and link to cognitive impairment
    Naila Ahmed
    Department of Biological Sciences, University of Essex, Colchester, Essex, UK
    J Neurochem 92:255-63. 2005
    ..A combination of nitration and glycation adduct estimates of CSF may provide an indicator for the diagnosis of Alzheimer's disease...
  64. ncbi request reprint Increased dicarbonyl metabolism in endothelial cells in hyperglycemia induces anoikis and impairs angiogenesis by RGD and GFOGER motif modification
    Darin Dobler
    Department of Biological Sciences, University of Essex, Colchester, Essex CO4 3SQ, UK
    Diabetes 55:1961-9. 2006
    ..Thus, increased formation of methylglyoxal and ECM glycation in hyperglycemia impairs endothelial cell survival and angiogenesis and likely contributes to similar vascular dysfunction in diabetes...