J O Thomas

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi HMG1 and 2: architectural DNA-binding proteins
    J O Thomas
    Cambridge Centre for Molecular Recognition and Department of Biochemistry, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Biochem Soc Trans 29:395-401. 2001
  2. ncbi HMG1 and 2, and related 'architectural' DNA-binding proteins
    J O Thomas
    Cambridge Centre for Molecular Recognition, University of Cambridge, 80 Tennis Court Road, Cambridge, UK CB2 1GA
    Trends Biochem Sci 26:167-74. 2001
  3. ncbi Histone H1: location and role
    J O Thomas
    Department of Biochemistry and Cambridge Centre for Molecular Recognition, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK
    Curr Opin Cell Biol 11:312-7. 1999
  4. ncbi Structure of the A-domain of HMG1 and its interaction with DNA as studied by heteronuclear three- and four-dimensional NMR spectroscopy
    C H Hardman
    Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK
    Biochemistry 34:16596-607. 1995
  5. ncbi Structural requirements for cooperative binding of HMG1 to DNA minicircles
    M Webb
    Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK
    J Mol Biol 309:79-88. 2001
  6. ncbi Photoaffinity labelling of a DNA-binding site on the globular domain of histone H5
    F A Goytisolo
    Cambridge Centre for Molecular Recognition, University of Cambridge, UK
    Eur J Biochem 242:619-26. 1996
  7. ncbi Selection of a cDNA clone for chicken high-mobility-group 1 (HMG1) protein through its unusually conserved 3'-untranslated region, and improved expression of recombinant HMG1 in Escherichia coli
    K B Lee
    Cambridge Centre for Molecular Recognition and Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Gene 225:97-105. 1998
  8. ncbi A sequence with homology to human HPFH-linked enhancer elements and to a family of G-protein linked membrane receptor genes is located downstream of the chicken beta-globin locus
    D M Staines
    Cambridge Centre for Molecular Recognition and Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Gene 234:345-52. 1999
  9. ncbi Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain
    A J Bannister
    Wellcome CRC Institute and Department of Pathology, University of Cambridge, UK
    Nature 410:120-4. 2001
  10. ncbi Structure of the HMG box motif in the B-domain of HMG1
    H M Weir
    Department of Biochemistry, University of Cambridge, UK
    EMBO J 12:1311-9. 1993

Collaborators

Detail Information

Publications11

  1. ncbi HMG1 and 2: architectural DNA-binding proteins
    J O Thomas
    Cambridge Centre for Molecular Recognition and Department of Biochemistry, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Biochem Soc Trans 29:395-401. 2001
    ..HMG1 and 2 appear to play important architectural roles in the assembly of nucleoprotein complexes in a variety of biological processes, for example V(D)J recombination, the initiation of transcription, and DNA repair...
  2. ncbi HMG1 and 2, and related 'architectural' DNA-binding proteins
    J O Thomas
    Cambridge Centre for Molecular Recognition, University of Cambridge, 80 Tennis Court Road, Cambridge, UK CB2 1GA
    Trends Biochem Sci 26:167-74. 2001
    ..HMG-box proteins might be targeted to particular DNA sites in chromatin by either protein-protein interactions or recognition of specific DNA structures...
  3. ncbi Histone H1: location and role
    J O Thomas
    Department of Biochemistry and Cambridge Centre for Molecular Recognition, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK
    Curr Opin Cell Biol 11:312-7. 1999
    ..The positioning of a core particle relative to a transcription factor binding-site may favour either transcription factor binding or H1 binding, depending on the location of the site...
  4. ncbi Structure of the A-domain of HMG1 and its interaction with DNA as studied by heteronuclear three- and four-dimensional NMR spectroscopy
    C H Hardman
    Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK
    Biochemistry 34:16596-607. 1995
    ..These contacts are very similar to those seen in the LEF-1 and SRY-DNA complexes [Love et al. (1995) Nature 376, 791-795; Werner et al. (1995) Cell 81, 705-714]...
  5. ncbi Structural requirements for cooperative binding of HMG1 to DNA minicircles
    M Webb
    Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK
    J Mol Biol 309:79-88. 2001
    ..Such effects could be relevant in the hierarchy of binding of HMG-box proteins to DNA distortions in vivo, where both single-box and two-box proteins might co-exist, with or without basic extensions and acidic tails...
  6. ncbi Photoaffinity labelling of a DNA-binding site on the globular domain of histone H5
    F A Goytisolo
    Cambridge Centre for Molecular Recognition, University of Cambridge, UK
    Eur J Biochem 242:619-26. 1996
    ..1994) Curr. Opin. Struct. Biol. 4. 44-50]...
  7. ncbi Selection of a cDNA clone for chicken high-mobility-group 1 (HMG1) protein through its unusually conserved 3'-untranslated region, and improved expression of recombinant HMG1 in Escherichia coli
    K B Lee
    Cambridge Centre for Molecular Recognition and Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Gene 225:97-105. 1998
    ..This should be general for all members of the HMG1 (and 2) family which may be toxic to cells (possibly because of the long acidic tail), and may also prove useful in the production of other such proteins...
  8. ncbi A sequence with homology to human HPFH-linked enhancer elements and to a family of G-protein linked membrane receptor genes is located downstream of the chicken beta-globin locus
    D M Staines
    Cambridge Centre for Molecular Recognition and Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Gene 234:345-52. 1999
    ..The existence of similar sequences at similar distances downstream of the beta-globin genes in chickens and HPFH patients is intriguing...
  9. ncbi Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain
    A J Bannister
    Wellcome CRC Institute and Department of Pathology, University of Cambridge, UK
    Nature 410:120-4. 2001
    ..This model may also explain the stable inheritance of the heterochromatic state...
  10. ncbi Structure of the HMG box motif in the B-domain of HMG1
    H M Weir
    Department of Biochemistry, University of Cambridge, UK
    EMBO J 12:1311-9. 1993
    ..The distinctive shape of the HMG box motif, which is distinct from hitherto characterized DNA-binding motifs, may be significant in relation to its recognition of four-way DNA junctions...
  11. ncbi A cDNA clone of the hnRNP C proteins and its homology with the single-stranded DNA binding protein UP2
    D K Lahiri
    Nucleic Acids Res 14:4077-94. 1986
    ..There is also a region that has a sequence very similar to two regions of the single-stranded DNA binding protein UP1 that contain proposed DNA binding sites...