Research Topics
Genomes and Genes | Gavin H ThomasSummaryAffiliation: University of York Country: UK Publications
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Detail Information
Publications
Evolutionary diversification of an ancient gene family (rhs) through C-terminal displacementAndrew P Jackson
The Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
BMC Genomics 10:584. 2009..coli. By comparing rhs repertoires across the Enterobacteriaceae, this study provides a robust explanation of rhs diversification and evolution, and a mechanistic model of how rhs diversity is gained and lost...
A fragile metabolic network adapted for cooperation in the symbiotic bacterium Buchnera aphidicolaGavin H Thomas
Department of Biology, University of York, PO Box 373, York, YO10 5YW, UK
BMC Syst Biol 3:24. 2009..coli genome...
Homes for the orphans: utilization of multiple substrate-binding proteins by ABC transportersGavin H Thomas
Department of Biology Area 10, University of York, York YO10 4BG, UK
Mol Microbiol 75:6-9. 2010..This new finding provides an important piece of in vivo data leading to further insight into how this ubiquitous family of transporters operates...
Furanose-specific sugar transport: characterization of a bacterial galactofuranose-binding proteinRichard S P Horler
Department of Biology, University of York, York YO10 5YW, United Kingdom
J Biol Chem 284:31156-63. 2009..coli, suggesting that this bacterium has evolved complementary transporters that enable it to use all the available galactose present during carbon limiting conditions...
Compensating stereochemical changes allow murein tripeptide to be accommodated in a conventional peptide-binding proteinAbbas Maqbool
Department of Biology Area 10, University of York, York YO10 5YW, United Kingdom
J Biol Chem 286:31512-21. 2011....
The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporterChristopher Mulligan
Department of Biology Area 10, University of York, P O Box 373, York YO10 5YW, United Kingdom
Proc Natl Acad Sci U S A 106:1778-83. 2009..Importantly, the binding protein also confers directionality on the transporter, and reversal of sialic acid transport from import to export is only possible in the presence of an excess of unliganded SiaP...
Characterization of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transportersEmmanuele Severi
Department of Biology, University of York, York, UK
FEMS Microbiol Lett 304:47-54. 2010....
Tripartite ATP-independent periplasmic transporters: application of a relational database for genome-wide analysis of transporter gene frequency and organizationChristopher Mulligan
Department of Biology, University of York, York, UK
J Mol Microbiol Biotechnol 12:218-26. 2007..Additionally, we report a number of new organizations of TRAP transporter genes and proteins which suggest the recruitment of TRAP transporter components for use in other biological contexts...
Sialic acid mutarotation is catalyzed by the Escherichia coli beta-propeller protein YjhTEmmanuele Severi
Department of Biology Area 10, York Structural Biology Laboratory, University of York, York YO10 5YW, United Kingdom
J Biol Chem 283:4841-9. 2008..We also present data suggesting that the ability to utilize alpha-N-acetylneuraminic acid released from complex sialoconjugates in vivo provides a physiological advantage to bacteria containing YjhT...
Caught in a TRAP: substrate-binding proteins in secondary transportMarcus Fischer
York Structural Biology Laboratory, Department of Chemistry, University of York, York, YO10 5DD, UK
Trends Microbiol 18:471-8. 2010..Other SBPs used in secondary transporters are structurally similar to ABC SBPs, demonstrating that multiple families of SBPs have evolved to function with secondary transporters...
MpaA is a murein-tripeptide-specific zinc carboxypeptidase that functions as part of a catabolic pathway for peptidoglycan-derived peptides in γ-proteobacteriaAbbas Maqbool
Department of Biology Area 10, Department of Chemistry, University of York, York YO10 5DD, UK
Biochem J 448:329-41. 2012....
Staphyloferrin A as siderophore-component in fluoroquinolone-based Trojan horse antibioticsStephen J Milner
Department of Chemistry, University of York, Heslington, York YO10 5DD, UK
Org Biomol Chem 11:3461-8. 2013..The loss of potency observed for the (R)- and (S)-ornithine-based ciprofloxacin conjugates correlates with their reduced inhibitory activity against the target enzyme DNA gyrase...
The membrane proteins SiaQ and SiaM form an essential stoichiometric complex in the sialic acid tripartite ATP-independent periplasmic (TRAP) transporter SiaPQM (VC1777-1779) from Vibrio choleraeChristopher Mulligan
Department of Biology Area 10, University of York, York YO10 5YW, United Kingdom
J Biol Chem 287:3598-608. 2012..Using reconstituted proteoliposomes containing particular combinations of the three subunits, we demonstrate biochemically that all three subunits are likely to be essential to form a functional TRAP transporter...
Tripartite ATP-independent periplasmic (TRAP) transporters in bacteria and archaeaChristopher Mulligan
Department of Biology, University of York, York, UK
FEMS Microbiol Rev 35:68-86. 2011....
The central role of the host cell in symbiotic nitrogen metabolismSandy J Macdonald
Department of Biology Area 10, University of York, York, UK
Proc Biol Sci 279:2965-73. 2012..Host cell-mediated nitrogen recycling may be general among insect symbioses with shared EAA biosynthetic pathways generated by the loss of symbiont genes mediating terminal reactions in EAA synthesis...
Sialic acid utilization by the soil bacterium Corynebacterium glutamicumNadine Gruteser
Department of Biology Area 10, University of York, York, UK
FEMS Microbiol Lett 336:131-8. 2012..The demonstration that a member of the Actinobacteria can transport and catabolize Neu5Ac efficiently suggests that sialic acid metabolism has a physiological role in the soil environment...
Sialic acid transport in Haemophilus influenzae is essential for lipopolysaccharide sialylation and serum resistance and is dependent on a novel tripartite ATP-independent periplasmic transporterEmmanuele Severi
Department of Biology, University of York, York, YO10 5YW, UK
Mol Microbiol 58:1173-85. 2005..The inactivation of the SiaPQM TRAP transporter also results in decreased growth of H. influenzae in a chemically defined medium containing Neu5Ac, supporting an additional nutritional role of sialic acid in H. influenzae physiology...
Synthesis of citrate-ciprofloxacin conjugatesSiti R Md-Saleh
Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK
Bioorg Med Chem Lett 19:1496-8. 2009..Cellular uptake mechanisms were investigated using wild-type and ompF deletion strains of Escherichia coli K-12...
BuchneraBASE: a post-genomic resource for Buchnera sp. APSM Dennis Prickett
Department of Biology, University of York, York YO10 5YW, UK
Bioinformatics 22:641-2. 2006..The database is designed to accommodate genome-wide post-genomic datasets that are becoming available for this organism. AVAILABILITY: BuchneraBASE is available at http://www.buchnera.org/...
Sialic acid utilization by bacterial pathogensEmmanuele Severi
Department of Biology Area 10, University of York, PO Box 373, York YO10 5YW, UK
Microbiology 153:2817-22. 2007..In this mini-review we will summarize recent findings relating to sialic acid transport, modification of sialic acid by O-acetylation, and the mechanisms of sialic acid-mediated complement resistance...
EchoLOCATION: an in silico analysis of the subcellular locations of Escherichia coli proteins and comparison with experimentally derived locationsRichard S P Horler
Department of Biology Area 10, University of York, York, UK
Bioinformatics 25:163-6. 2009..From analysis of disagreements between in silico predictions and experimental data, we provide an analysis of protein types where subcellular prediction algorithms are currently not accurate...
An ATP-binding cassette-type cysteine transporter in Campylobacter jejuni inferred from the structure of an extracytoplasmic solute receptor proteinAxel Muller
Structural Biology Laboratory, Department of Chemistry, University of York, York, YO10 5YW, UK
Mol Microbiol 57:143-55. 2005..These data imply that Cj0982 is the binding protein component of an ABC-type cysteine transporter system and that cysteine uptake is important in the physiology of C. jejuni...
EchoBASE: an integrated post-genomic database for Escherichia coliRaju V Misra
Department of Biology (Area 10, University of York, PO Box 373, York, YO10 5YW, UK
Nucleic Acids Res 33:D329-33. 2005..EchoBASE also contains annotated information on 'orphan' enzyme activities from this microbe to aid characterization of the proteins that catalyse these elusive biochemical reactions...
