Yasuhiro Takeuchi

Summary

Affiliation: University College London
Country: UK

Publications

  1. pmc Suboptimal porcine endogenous retrovirus infection in non-human primate cells: implication for preclinical xenotransplantation
    Giada Mattiuzzo
    Division of Infection and Immunity, Wohl Virion Centre, University College London, London, United Kingdom
    PLoS ONE 5:e13203. 2010
  2. pmc Recombination and population mosaic of a multifunctional viral gene, adeno-associated virus cap
    Yasuhiro Takeuchi
    Medical Reserach Council MRC UCL Centre for Medical Molecular Virology, Division of Infection and Immunology, University College London, London, United Kingdom
    PLoS ONE 3:e1634. 2008
  3. pmc Evidence and consequence of porcine endogenous retrovirus recombination
    Birke Bartosch
    Wohl Virion Centre, Division of Infection of Immunity, University College London, 46 Cleveland St, London W1T 4JF, United Kingdom
    J Virol 78:13880-90. 2004
  4. pmc Simian immunodeficiency virus envelope glycoprotein counteracts tetherin/BST-2/CD317 by intracellular sequestration
    Ravindra K Gupta
    Medical Research Council Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, 46 Cleveland Street, London W1T 4JF, United Kingdom
    Proc Natl Acad Sci U S A 106:20889-94. 2009
  5. pmc Porcine endogenous retroviruses PERV A and A/C recombinant are insensitive to a range of divergent mammalian TRIM5alpha proteins including human TRIM5alpha
    Andrew Wood
    MRC Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, 46 Cleveland Street, London W1T 4JF, UK
    J Gen Virol 90:702-9. 2009
  6. pmc Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species
    Giada Mattiuzzo
    Wohl Virion Centre, Division of Infection and Immunity, University College London, W1T 4JF, London, UK
    Retrovirology 4:93. 2007
  7. doi request reprint A lentiviral vector pseudotype suitable for vaccine development
    Luciene Lopes
    Immunity and Infection, University College London, London, UK
    J Gene Med 13:181-7. 2011
  8. pmc Effect of the internal promoter on insertional gene activation by lentiviral vectors with an intact HIV long terminal repeat
    Sean Knight
    Division of Infection and Immunity, University College London, 46 Cleveland St, London W1T 4JF, United Kingdom
    J Virol 84:4856-9. 2010
  9. ncbi request reprint Insertional mutagenesis by retroviral vectors: current concepts and methods of analysis
    Sean Knight
    MRC UCL Centre for Medical Molecular Virology and Wohl Virion Centre, Division of Infection and Immunity, UCL Cancer Institute, University College London, London, UK
    Curr Gene Ther 13:211-27. 2013
  10. pmc Regulation of porcine endogenous retrovirus release by porcine and human tetherins
    Giada Mattiuzzo
    Wohl Virion Centre, Division of Infection and Immunity, University College London, London, United Kingdom
    J Virol 84:2618-22. 2010

Collaborators

Detail Information

Publications41

  1. pmc Suboptimal porcine endogenous retrovirus infection in non-human primate cells: implication for preclinical xenotransplantation
    Giada Mattiuzzo
    Division of Infection and Immunity, Wohl Virion Centre, University College London, London, United Kingdom
    PLoS ONE 5:e13203. 2010
    ..We investigated the mechanism responsible for the low efficiency of PERV-A infection in NHP cells...
  2. pmc Recombination and population mosaic of a multifunctional viral gene, adeno-associated virus cap
    Yasuhiro Takeuchi
    Medical Reserach Council MRC UCL Centre for Medical Molecular Virology, Division of Infection and Immunology, University College London, London, United Kingdom
    PLoS ONE 3:e1634. 2008
    ..Additionally the possible biological significance of two dominant VP1u forms is inferred...
  3. pmc Evidence and consequence of porcine endogenous retrovirus recombination
    Birke Bartosch
    Wohl Virion Centre, Division of Infection of Immunity, University College London, 46 Cleveland St, London W1T 4JF, United Kingdom
    J Virol 78:13880-90. 2004
    ..In this context, pigs lacking non-human-tropic PERV-C would be more suitable as donor animals for clinical xenotransplantation...
  4. pmc Simian immunodeficiency virus envelope glycoprotein counteracts tetherin/BST-2/CD317 by intracellular sequestration
    Ravindra K Gupta
    Medical Research Council Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, 46 Cleveland Street, London W1T 4JF, United Kingdom
    Proc Natl Acad Sci U S A 106:20889-94. 2009
    ..Our observations emphasize the importance of tetherin in protecting mammals against viral infection and suggest that HIV-1 Vpu inhibitors may select active envelope mutants...
  5. pmc Porcine endogenous retroviruses PERV A and A/C recombinant are insensitive to a range of divergent mammalian TRIM5alpha proteins including human TRIM5alpha
    Andrew Wood
    MRC Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, 46 Cleveland Street, London W1T 4JF, UK
    J Gen Virol 90:702-9. 2009
    ..We conclude that xenotransplantation studies should consider the danger of highly infectious TRIM5alpha-insensitive human-tropic PERV recombinants...
  6. pmc Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species
    Giada Mattiuzzo
    Wohl Virion Centre, Division of Infection and Immunity, University College London, W1T 4JF, London, UK
    Retrovirology 4:93. 2007
    ..Here, we characterised human PARs and their homologues in the PERV-A resistant rodent species, mouse and rat (muPAR and ratPAR, respectively)...
  7. doi request reprint A lentiviral vector pseudotype suitable for vaccine development
    Luciene Lopes
    Immunity and Infection, University College London, London, UK
    J Gene Med 13:181-7. 2011
    ..The present study aimed to find a suitable envelope for LV vaccine production from stable packaging cells because the commonly used vesicular stomatitis virus envelope (VSV-G) is cytotoxic...
  8. pmc Effect of the internal promoter on insertional gene activation by lentiviral vectors with an intact HIV long terminal repeat
    Sean Knight
    Division of Infection and Immunity, University College London, 46 Cleveland St, London W1T 4JF, United Kingdom
    J Virol 84:4856-9. 2010
    ..We also show that there are more transcripts originating from the 5' LTR than from, or reading through, the 3' LTR. This study will allow the design of safer lentiviral vectors for applications in which an intact HIV LTR is required...
  9. ncbi request reprint Insertional mutagenesis by retroviral vectors: current concepts and methods of analysis
    Sean Knight
    MRC UCL Centre for Medical Molecular Virology and Wohl Virion Centre, Division of Infection and Immunity, UCL Cancer Institute, University College London, London, UK
    Curr Gene Ther 13:211-27. 2013
    ..Old and new methods for assessing this phenomenon are discussed with a view to provide a comprehensive account of this emerging field...
  10. pmc Regulation of porcine endogenous retrovirus release by porcine and human tetherins
    Giada Mattiuzzo
    Wohl Virion Centre, Division of Infection and Immunity, University College London, London, United Kingdom
    J Virol 84:2618-22. 2010
    ....
  11. doi request reprint Potential zoonotic infection of porcine endogenous retrovirus in xenotransplantation
    Giada Mattiuzzo
    Division of Infection and Immunity, Wohl Virion Centre, University College London, London, UK
    Methods Mol Biol 885:263-79. 2012
    ..Finally, it is important to determine the presence of critical PERV loci involved in transmission in the pig genome and compare between different animals. One of the methods for the analysis of these PERV integration sites is described...
  12. doi request reprint A sensitive retroviral pseudotype assay for influenza H5N1-neutralizing antibodies
    Nigel J Temperton
    MRC UCL Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, 46 Cleveland Street, London, United Kingdom
    Influenza Other Respir Viruses 1:105-12. 2007
    ..We have used retroviral pseudotypes bearing influenza H5 hemagglutinin (HA) as safe, surrogate viruses for influenza neutralization assays which can be carried out at Biosafety Level 2...
  13. doi request reprint Strategies to enhance the safety profile of xenotransplantation: minimizing the risk of viral zoonoses
    Giada Mattiuzzo
    Wohl Virion Centre, Division of Infection and Immunity, University College London, London, UK
    Curr Opin Organ Transplant 13:184-8. 2008
    ..Safety issues regarding viral zoonosis, particularly porcine endogenous retroviruses, are summarized and commented upon...
  14. ncbi request reprint PCR-based cloning and immunocytological titration of infectious porcine endogenous retrovirus subgroup A and B
    Birke Bartosch
    Wohl Virion Centre, The Windeyer Institute of Medical Sciences, University College London, 46 Cleveland Street, London W1T 4JF, UK
    J Gen Virol 83:2231-40. 2002
    ..This detection method proved to be more sensitive than the current method of choice (transfer of MLV-lacZ vectors) for infectivity assessment of PERV. These findings should be considered for future characterization of PERV isolates...
  15. pmc Safer, silencing-resistant lentiviral vectors: optimization of the ubiquitous chromatin-opening element through elimination of aberrant splicing
    Sean Knight
    MRC UCL Centre for Medical Molecular Virology and Wohl Virion Centre, Division of Infection and Immunity, UCL Cancer Institute, University College London, London, United Kingdom
    J Virol 86:9088-95. 2012
    ..These refined A2UCOE regulatory elements are likely to improve intrinsic biosafety and may be particularly useful for a number of clinical applications where robust gene expression is desirable...
  16. pmc Human immunodeficiency virus type 1 vectors with alphavirus envelope glycoproteins produced from stable packaging cells
    Blair L Strang
    Department of Immunology and Molecular Pathology, University College London, University of London, London, United Kingdom
    J Virol 79:1765-71. 2005
    ....
  17. pmc The β-globin locus control region in combination with the EF1α short promoter allows enhanced lentiviral vector-mediated erythroid gene expression with conserved multilineage activity
    Claudia A Montiel-Equihua
    Centre for Immunodeficiency, Molecular Immunology Unit, Institute of Child Health, University College London, London, UK
    Mol Ther 20:1400-9. 2012
    ....
  18. pmc Reduced sensitivity to human serum inactivation of enveloped viruses produced by pig cells transgenic for human CD55 or deficient for the galactosyl-alpha(1-3) galactosyl epitope
    Saema Magre
    Wohl Virion Centre, Windeyer Institute of Medical Sciences, University College London, 46 Cleveland St, London W1T 4JF, United Kingdom
    J Virol 78:5812-9. 2004
    ..Additionally, VSV from Gal-null pig cells was resistant to human complement inactivation. Such protection of enveloped viruses may increase the risk of zoonosis from pigs genetically modified for pig-to-human xenotransplantation...
  19. ncbi request reprint Xenotransplantation and pig endogenous retroviruses
    Saema Magre
    Wohl Virion Centre, The Windeyer Institute of Medical Sciences, University College London, 46 Cleveland Street, London W1T 4JF, UK
    Rev Med Virol 13:311-29. 2003
    ....
  20. pmc Murine leukemia virus particle assembly quantitated by fluorescence microscopy: role of Gag-Gag interactions and membrane association
    Mariam Andrawiss
    Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Science, University College London, London, United Kingdom
    J Virol 77:11651-60. 2003
    ..These data suggest that two independent mechanisms, CA interactions and membrane association following myristylation, cooperate in MLV Gag assembly and budding...
  21. pmc Characterization of murine leukemia virus restriction in mammals
    Caroline Besnier
    Wohl Virion Centre, Department of Immunology and Molecular Pathology, University College London, London W1T 4JF, United Kingdom
    J Virol 77:13403-6. 2003
    ..We demonstrate restriction in cells from bat and show that if we express the murine restriction factor Fv1 in human cells, then Fv1, not the human host, defines the stage at which infection is blocked...
  22. pmc Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes
    Nigel J Temperton
    Wohl Virion Centre and Division of Infection and Immunity, University College London, 46 Cleveland St, London W1T 4JF, London, UK
    Emerg Infect Dis 11:411-6. 2005
    ..The pseudotype assay does not require handling live SARS virus; it is a useful tool to determine neutralizing titers during natural infection and the preclinical evaluation of candidate vaccines...
  23. ncbi request reprint Continuous high-titer HIV-1 vector production
    Yasuhiro Ikeda
    Department of Immunology and Molecular Pathology, Windeyer Institute, University College London, 46 Cleveland St, London W1T 4JF, UK
    Nat Biotechnol 21:569-72. 2003
    ..u.)/ml (20 293T i.u./cell/day) for at least three months in culture...
  24. pmc Identification of gammaretroviruses constitutively released from cell lines used for human immunodeficiency virus research
    Yasuhiro Takeuchi
    Wohl Virion Centre, Division of Infection and Immunity, University College London, London W1T 4JF, United Kingdom
    J Virol 82:12585-8. 2008
    ..01/F7 cells to produce gibbon ape leukemia virus. These findings highlight the importance of routine testing of cell lines for retrovirus contamination to prevent potential experimental artifacts and allow correct biohazard assessment...
  25. pmc Insertional gene activation by lentiviral and gammaretroviral vectors
    Marieke Bokhoven
    Infection and Immunity, University College London, Windeyer Building, 46 Cleveland Street, London W1T 2AH, United Kingdom
    J Virol 83:283-94. 2009
    ..In contrast, gammaretroviral vectors insert into other loci, including IL-3 and genes identified as common insertion sites in the Retroviral Tagged Cancer Gene Database (RTCGD)...
  26. pmc Abrogation of Ref1 retrovirus restriction in human cells
    Greg Towers
    Wohl Virion Centre, Department of Immunology and Molecular Pathology, University College London, London W1T 4JF, United Kingdom
    J Virol 76:2548-50. 2002
    ..Restricted infection kinetics are multiple hit at high virus dose, and restriction is abrogated by preexposure to restricted virus. We hypothesize that restricted capsid can titrate out the restriction factor...
  27. pmc Restriction of lentivirus in monkeys
    Caroline Besnier
    Wohl Virion Centre, Department of Immunology and Molecular Pathology, University College London, 46 Cleveland Street, London W1T 4JF, United Kingdom
    Proc Natl Acad Sci U S A 99:11920-5. 2002
    ..Intriguingly, African green monkey cells are able to block both HIV and simian immunodeficiency virus, and each virus is able to saturate and abrogate the restriction of the other, suggesting that a common factor is responsible...
  28. pmc Heparin binds to murine leukemia virus and inhibits Env-independent attachment and infection
    Simon J Walker
    Department of Haematology, University College, London, United Kingdom
    J Virol 76:6909-18. 2002
    ..Although the molecular mechanism is unclear, this study presents evidence that Env receptor-independent attachment is an important step in MLV infection...
  29. ncbi request reprint Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells
    Richard J Vart
    Cancer Research U K Viral Oncology Group, Wolfson Institute for Biomedical Research, University College London, London, United Kingdom
    Cancer Res 67:4042-51. 2007
    ..These findings support the paracrine and autocrine roles of the lytic KSHV-encoded proteins, vIL6 and vGPCR, in KS pathogenesis and identify Ang2 as a potential therapeutic target for this neoplasm...
  30. pmc Xenotransplantation-associated infectious risk: a WHO consultation
    Jay A Fishman
    Transplantation Infectious Disease and Compromised Host Program, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Xenotransplantation 19:72-81. 2012
    ..This document summarizes approaches to disease surveillance in individual recipients of nonhuman tissues...
  31. ncbi request reprint Decreased AP-1 activity and interleukin-11 expression by bone marrow stromal cells may be associated with impaired bone formation in aged mice
    Emiko Tohjima
    Department of Medicine and Bioregulatory Sciences, University of Tokushima Graduate School of Medicine, Tokushima, Japan
    J Bone Miner Res 18:1461-70. 2003
    ..Therefore, diminished AP-1 activity and the resultant decline in IL-11 expression may play a role in impaired bone formation in the aged...
  32. pmc Determinants of high titer in recombinant porcine endogenous retroviruses
    Ian Harrison
    National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    J Virol 78:13871-9. 2004
    ..Taken together, these results show that the novel juxtaposition of env gene sequences enhanced the infectivity of PERV-A14/220 for human cells, perhaps by stabilization of the envelope glycoprotein or increased receptor binding...
  33. ncbi request reprint Molecular cloning, complete sequence, and biological characterization of a xenotropic murine leukemia virus constitutively released from the human B-lymphoblastoid cell line DG-75
    Kevin P Raisch
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Virology 308:83-91. 2003
    ..Viral interference and infectivity assays confirmed the xenotropic nature of the DG-75 retrovirus. The DG-75 retrovirus is the first isolate of an exogenous xenotropic MLV in which the full-length genomic sequence has been characterized...
  34. pmc Identification of receptors for pig endogenous retrovirus
    Thomas A Ericsson
    Immerge BioTherapeutics, Inc, 300 Technology Square, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 100:6759-64. 2003
    ..The identification of the PERV-A receptors opens avenues of research necessary for a more complete assessment of the retroviral risks of pig to human xenotransplantation...
  35. pmc Tubulovesicular structures within vesicular stomatitis virus G protein-pseudotyped lentiviral vector preparations carry DNA and stimulate antiviral responses via Toll-like receptor 9
    Andreas Pichlmair
    Immunobiology Laboratory, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Virol 81:539-47. 2007
    ....
  36. ncbi request reprint Ghrelin directly regulates bone formation
    Nobuhiro Fukushima
    Molecular Genetics, Institute of Life Science, Kurume University, Kurume, Fukuoka, Japan
    J Bone Miner Res 20:790-8. 2005
    ..Ghrelin and its receptor, GHS-R1a, were identified in osteoblasts, and ghrelin promoted both proliferation and differentiation. Furthermore, ghrelin increased BMD in rats. Our results show that ghrelin directly affects bone formation...
  37. ncbi request reprint Cytokines suppress adipogenesis and PPAR-gamma function through the TAK1/TAB1/NIK cascade
    Miyuki Suzawa
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Nat Cell Biol 5:224-30. 2003
    ....
  38. ncbi request reprint Clonal endothelial cells produce humoral factors that inhibit osteoclast-like cell formation in vitro
    Noriko Chikatsu
    Department of Medicine, University of Tokyo School of Medicine, Japan
    Endocr J 49:439-47. 2002
    ..Hence, endothelial cells in bone may inhibit recruitment of fresh osteoclasts, and those in tissues other than bone may be involved in prohibiting ectopic osteoclastogenesis...
  39. ncbi request reprint Use of CD134 as a primary receptor by the feline immunodeficiency virus
    Masayuki Shimojima
    Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Science 303:1192-5. 2004
    ..Thus, despite the evolutionary divergence of the feline and human lentiviruses, both viruses use receptors that target the virus to a subset of cells that are pivotal to the acquired immune response...
  40. ncbi request reprint Gene transfer to repopulating human CD34+ cells using amphotropic-, GALV-, or RD114-pseudotyped HIV-1-based vectors from stable producer cells
    Thomas Relander
    Department of Molecular Medicine and Gene Therapy, Lund University, 221 84 Lund, Sweden
    Mol Ther 11:452-9. 2005
    ..1% of NOD/SCID repopulating human cells, which was significantly higher than the MLV-A (12.6%)- or GALV+ (25.1%)-pseudotyped vectors...
  41. ncbi request reprint Dose-dependent biochemical changes in rat central nervous system after 12-week exposure to 1-bromopropane
    Hailan Wang
    Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Japan
    Neurotoxicology 24:199-206. 2003
    ..The biochemical changes in the cerebrum indicate that long-term exposure to 1-bromopropane has effects on the CNS...