V V Sumbayev

Summary

Affiliation: University of Kent
Country: UK

Publications

  1. ncbi request reprint Regulation of MAP kinase-dependent apoptotic pathway: implication of reactive oxygen and nitrogen species
    Vadim V Sumbayev
    Department of Biochemistry, Mechnikov Odessa National University, Odessa, Ukraine
    Arch Biochem Biophys 436:406-12. 2005
  2. doi request reprint Gene silencing approaches in mast cells and primary human basophils
    Vadim V Sumbayev
    Medway School of Pharmacy, The University of Kent, Anson Building, Central Avenue, Chatham Maritime, Kent, ME4 4TB, UK
    Methods Mol Biol 1192:185-91. 2014
  3. pmc Effects of stem cell factor on hypoxia-inducible factor 1 alpha accumulation in human acute myeloid leukaemia and LAD2 mast cells
    Bernhard F Gibbs
    Medway School of Pharmacy, University of Kent, Kent, United Kingdom
    PLoS ONE 6:e22502. 2011
  4. pmc Differential role of hypoxia-inducible factor 1 alpha in toll-like receptor-mediated and allergic inflammatory reactions
    Vadim V Sumbayev
    From the University of Kent, Chatham Maritime, Kent, United Kingdom
    World Allergy Organ J 3:245-9. 2010
  5. doi request reprint Gold nanoparticles downregulate interleukin-1β-induced pro-inflammatory responses
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Chatham Maritime, Kent, UK
    Small 9:472-7. 2013
  6. pmc Involvement of hypoxia-inducible factor-1 in the inflammatory responses of human LAD2 mast cells and basophils
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Chatham Maritime, United Kingdom
    PLoS ONE 7:e34259. 2012
  7. doi request reprint Hypoxia-inducible factor 1 as one of the "signaling drivers" of Toll-like receptor-dependent and allergic inflammation
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Anson Building, Central Avenue, Chatham Maritime, Kent ME44TB, UK
    Arch Immunol Ther Exp (Warsz) 58:287-94. 2010
  8. doi request reprint Involvement of hypoxia-inducible factor-1 HiF(1alpha) in IgE-mediated primary human basophil responses
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Chatham Maritime, Kent, UK
    Eur J Immunol 39:3511-9. 2009
  9. ncbi request reprint LPS-induced Toll-like receptor 4 signalling triggers cross-talk of apoptosis signal-regulating kinase 1 (ASK1) and HIF-1alpha protein
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Anson Building, Central Avenue, Chatham Maritime, Kent, United Kingdom
    FEBS Lett 582:319-26. 2008
  10. ncbi request reprint PI3 kinase and direct S-nitrosation are involved in down-regulation of apoptosis signal-regulating kinase 1 during LPS-induced Toll-like receptor 4 signalling
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Anson Building, Central Avenue, Chatham Maritime, Kent ME4 4TB, United Kingdom
    Immunol Lett 115:126-30. 2008

Collaborators

Detail Information

Publications20

  1. ncbi request reprint Regulation of MAP kinase-dependent apoptotic pathway: implication of reactive oxygen and nitrogen species
    Vadim V Sumbayev
    Department of Biochemistry, Mechnikov Odessa National University, Odessa, Ukraine
    Arch Biochem Biophys 436:406-12. 2005
    ..Also we have outlined other recently discovered signal transduction processes involved in the regulation of ASK1 activity and downstream pathway...
  2. doi request reprint Gene silencing approaches in mast cells and primary human basophils
    Vadim V Sumbayev
    Medway School of Pharmacy, The University of Kent, Anson Building, Central Avenue, Chatham Maritime, Kent, ME4 4TB, UK
    Methods Mol Biol 1192:185-91. 2014
    ..Here, we describe a method using lipofection which has been successfully employed to silence gene expression using siRNA in human LAD2 mast cells as well as primary human basophils. ..
  3. pmc Effects of stem cell factor on hypoxia-inducible factor 1 alpha accumulation in human acute myeloid leukaemia and LAD2 mast cells
    Bernhard F Gibbs
    Medway School of Pharmacy, University of Kent, Kent, United Kingdom
    PLoS ONE 6:e22502. 2011
    ..These results demonstrate for the first time an important cross-talk of signalling pathways associated with HIF-1 activation--an important stage of the myeloid leukaemia cell life cycle...
  4. pmc Differential role of hypoxia-inducible factor 1 alpha in toll-like receptor-mediated and allergic inflammatory reactions
    Vadim V Sumbayev
    From the University of Kent, Chatham Maritime, Kent, United Kingdom
    World Allergy Organ J 3:245-9. 2010
    ..Importantly, the HIF-1 transcription complex is activated in human basophils during IgE-mediated inflammatory responses. It is involved in VEGF expression and subsequent promotion of angiogenesis and in controlling energy metabolism...
  5. doi request reprint Gold nanoparticles downregulate interleukin-1β-induced pro-inflammatory responses
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Chatham Maritime, Kent, UK
    Small 9:472-7. 2013
    ..Our results indicate that the anti-inflammatory activity of gold nanoparticles is associated with an extracellular interaction with IL-1β, thus opening potentially novel options for further therapeutic applications...
  6. pmc Involvement of hypoxia-inducible factor-1 in the inflammatory responses of human LAD2 mast cells and basophils
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Chatham Maritime, United Kingdom
    PLoS ONE 7:e34259. 2012
    ..This transcription complex facilitates generation of both pro-angiogenic and inflammatory cytokines in mast cells but has a differential role in basophil stimulation comparing IgE-dependent triggering with innate immune stimuli...
  7. doi request reprint Hypoxia-inducible factor 1 as one of the "signaling drivers" of Toll-like receptor-dependent and allergic inflammation
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Anson Building, Central Avenue, Chatham Maritime, Kent ME44TB, UK
    Arch Immunol Ther Exp (Warsz) 58:287-94. 2010
    ..Differential biochemical mechanisms employed to stabilize the protein in different cases are discussed...
  8. doi request reprint Involvement of hypoxia-inducible factor-1 HiF(1alpha) in IgE-mediated primary human basophil responses
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Chatham Maritime, Kent, UK
    Eur J Immunol 39:3511-9. 2009
    ..In addition, HIF-1alpha protein alters IgE-induced ATP depletion in basophils, thus also supporting the production of the pro-allergic cytokine IL-4...
  9. ncbi request reprint LPS-induced Toll-like receptor 4 signalling triggers cross-talk of apoptosis signal-regulating kinase 1 (ASK1) and HIF-1alpha protein
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Anson Building, Central Avenue, Chatham Maritime, Kent, United Kingdom
    FEBS Lett 582:319-26. 2008
    ..ASK1 contributes to the stabilisation of HIF-1alpha, most likely via activation of p38 MAP kinase...
  10. ncbi request reprint PI3 kinase and direct S-nitrosation are involved in down-regulation of apoptosis signal-regulating kinase 1 during LPS-induced Toll-like receptor 4 signalling
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, Anson Building, Central Avenue, Chatham Maritime, Kent ME4 4TB, United Kingdom
    Immunol Lett 115:126-30. 2008
    ..In addition, nitric oxide produced in response to exposure of THP-1 cells to LPS was found to S-nitrosate and therefore, down-regulate ASK1 activity...
  11. ncbi request reprint Mechanisms of hypoxic signal transduction regulated by reactive nitrogen species
    V V Sumbayev
    Medway School of Pharmacy, University of Kent and Greenwich, Kent, UK
    Scand J Immunol 65:399-406. 2007
    ....
  12. ncbi request reprint HIF-1 alpha protein as a target for S-nitrosation
    Vadim V Sumbayev
    University of Kaiserslautern, Faculty of Biology, Department of Cell Biology, Erwin Schrodinger Strasse, 67663 Kaiserslautern, Germany
    FEBS Lett 535:106-12. 2003
    ..In conclusion, HIF-1 alpha is a target for S-nitrosation by exogenously and endogenously produced NO...
  13. ncbi request reprint Oxidized low-density lipoprotein (oxLDL) triggers hypoxia-inducible factor-1alpha (HIF-1alpha) accumulation via redox-dependent mechanisms
    Vladimir A Shatrov
    Faculty of Biology, Department of Cell Biology, University of Kaiserslautern, Germany
    Blood 101:4847-9. 2003
    ..This finding may suggest a role of HIF-1 in atherosclerosis and oxLDL-induced pathogenesis...
  14. ncbi request reprint S-nitrosylation of thioredoxin mediates activation of apoptosis signal-regulating kinase 1
    Vadim V Sumbayev
    Department of Biochemistry, Mechnikov Odessa National University, Apt 208, Glushko Prospect 16, Odessa 65104, Ukraine
    Arch Biochem Biophys 415:133-6. 2003
    ..These results suggest that S-nitrosylation of Trx induces ASK1 activation. Treatment of cells with N-acetyl-cysteine for 2h after 8h of pretreatment with GSNO caused an increase in glutathione and nullified ASK1 activation...
  15. ncbi request reprint S-nitrosation of thioredoxin in the nitrogen monoxide/superoxide system activates apoptosis signal-regulating kinase 1
    Inna M Yasinska
    Department of Biochemistry, Mechnikov Odessa National University, Per Shampansky 2, Odessa 65058, Ukraine
    Arch Biochem Biophys 428:198-203. 2004
    ..However, approximately 4-fold of NO excess compared to a superoxide production preserved the level of dissociated ASK1 but decreased its activity due to the enzyme S-nitrosation...
  16. doi request reprint HIF-1alpha protein is an essential factor for protection of myeloid cells against LPS-induced depletion of ATP and apoptosis that supports Toll-like receptor 4-mediated production of IL-6
    Harjinder Lall
    Medway School of Pharmacy, University of Kent, United Kingdom
    Mol Immunol 45:3045-9. 2008
    ..On the other hand HIF-1alpha protein is selectively required for down-regulation of ASK1 activated during LPS-induced TLR4 downstream signalling...
  17. ncbi request reprint S-nitrosation of Cys-800 of HIF-1alpha protein activates its interaction with p300 and stimulates its transcriptional activity
    Inna M Yasinska
    Department of Biochemistry, Mechnikov Odessa National University, Apt 208, Glushko Prospect 16, Odessa 65104, Ukraine
    FEBS Lett 549:105-9. 2003
    ..Experiments with GST pull-down assay suggest that S-nitrosation of Cys-800 stimulates the recruitment of p300 co-activator protein to the HIF-1alpha C-terminal domain...
  18. doi request reprint Protein S-nitrosation in signal transduction: assays for specific qualitative and quantitative analysis
    Vadim V Sumbayev
    Medway School of Pharmacy, University of Kent, United Kingdom
    Methods Enzymol 440:209-19. 2008
    ..This chapter describes and discusses recently developed methods that allow both qualitative and quantitative analyses of S-nitrosation of intracellular proteins based on Western blot and enzyme-linked immunosorbent assay...
  19. ncbi request reprint Nitric oxide inhibits HIF-1alpha protein accumulation under hypoxic conditions: implication of 2-oxoglutarate and iron
    Anna V Kozhukhar
    Department of Obstetrics and Gynecology, Odessa State Medical University, Marshala Govorova Street 28, Odessa 65009, Ukraine
    Biochimie 88:411-8. 2006
    ..Treatment of those cells with N-acetyl cysteine (NAC) increased the amount of intracellular GSH and attenuated the NO effect and abolished the damage of mitochondria as well as 2-OG/iron release...
  20. ncbi request reprint Mesangial cells but not hepatocytes are protected against NO/O(2)(-) cogeneration: mechanistic considerations
    Vadim V Sumbayev
    Department of Cell Biology, Faculty of Biology, University of Kaiserslautern, Erwin Schrodinger Strasse, 67663, Kaiserslautern, Germany
    Eur J Pharmacol 444:1-11. 2002
    ..In conclusion, NO/O(2)(-) sensitivity is cell type specific and is determined by the glutathione redox system...