Murray Stewart

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi request reprint Molecular mechanism of translocation through nuclear pore complexes during nuclear protein import
    M Stewart
    MRC Laboratory of Molecular Biology, Hills Rd, CB2 2QH, Cambridge, UK
    FEBS Lett 498:145-9. 2001
  2. ncbi request reprint Molecular mechanism of the nuclear protein import cycle
    Murray Stewart
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Rev Mol Cell Biol 8:195-208. 2007
  3. ncbi request reprint Structural biology. Nuclear trafficking
    Murray Stewart
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, UK
    Science 302:1513-4. 2003
  4. ncbi request reprint Structural basis for the nuclear protein import cycle
    M Stewart
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Biochem Soc Trans 34:701-4. 2006
  5. ncbi request reprint Ratcheting mRNA out of the nucleus
    Murray Stewart
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK
    Mol Cell 25:327-30. 2007
  6. ncbi request reprint Insights into the molecular mechanism of nuclear trafficking using nuclear transport factor 2 (NTF2)
    M Stewart
    Medical Research Council Laboratory of Molecular Biology, Cambridge, England
    Cell Struct Funct 25:217-25. 2000
  7. ncbi request reprint 1.9 A resolution crystal structure of the Saccharomyces cerevisiae Ran-binding protein Mog1p
    M Stewart
    MRC Laboratory of Molecular Biology, Cambridge, England
    J Mol Biol 299:213-23. 2000
  8. doi request reprint Nuclear export of mRNA
    Murray Stewart
    MRC Laboratory of Molecular Biology, Hills Rd, Cambridge CB2 0QH, UK
    Trends Biochem Sci 35:609-17. 2010
  9. ncbi request reprint Nuclear pores and macromolecular assemblies involved in nucleocytoplasmic transport
    M Stewart
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Curr Opin Struct Biol 6:162-5. 1996
  10. pmc Structural basis for the interaction between yeast Spt-Ada-Gcn5 acetyltransferase (SAGA) complex components Sgf11 and Sus1
    Andrew M Ellisdon
    From the Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom and
    J Biol Chem 285:3850-6. 2010

Collaborators

Detail Information

Publications56

  1. ncbi request reprint Molecular mechanism of translocation through nuclear pore complexes during nuclear protein import
    M Stewart
    MRC Laboratory of Molecular Biology, Hills Rd, CB2 2QH, Cambridge, UK
    FEBS Lett 498:145-9. 2001
    ....
  2. ncbi request reprint Molecular mechanism of the nuclear protein import cycle
    Murray Stewart
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Rev Mol Cell Biol 8:195-208. 2007
    ....
  3. ncbi request reprint Structural biology. Nuclear trafficking
    Murray Stewart
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, UK
    Science 302:1513-4. 2003
  4. ncbi request reprint Structural basis for the nuclear protein import cycle
    M Stewart
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Biochem Soc Trans 34:701-4. 2006
    ..Interactions between transport factors and key nucleoporins (such as Nup1p, Nup2p and Nup50) accelerate the formation and dissolution of many of these complexes...
  5. ncbi request reprint Ratcheting mRNA out of the nucleus
    Murray Stewart
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK
    Mol Cell 25:327-30. 2007
    ..In budding yeast, recent work suggests that the DEAD-box helicase Dbp5 remodels mRNPs at the NPC cytoplasmic face by removing Mex67 and that the Dbp5 ATPase is activated by Gle1 and inositol hexaphosphate (IP(6))...
  6. ncbi request reprint Insights into the molecular mechanism of nuclear trafficking using nuclear transport factor 2 (NTF2)
    M Stewart
    Medical Research Council Laboratory of Molecular Biology, Cambridge, England
    Cell Struct Funct 25:217-25. 2000
    ..An analogous nucleoporin hopping mechanism may also be used by carrier molecules of the importin-beta family to move through NPCs...
  7. ncbi request reprint 1.9 A resolution crystal structure of the Saccharomyces cerevisiae Ran-binding protein Mog1p
    M Stewart
    MRC Laboratory of Molecular Biology, Cambridge, England
    J Mol Biol 299:213-23. 2000
    ....
  8. doi request reprint Nuclear export of mRNA
    Murray Stewart
    MRC Laboratory of Molecular Biology, Hills Rd, Cambridge CB2 0QH, UK
    Trends Biochem Sci 35:609-17. 2010
    ..Recent structural and cell biology results provide key information that should enable development of a detailed understanding of this central cellular process at a molecular level...
  9. ncbi request reprint Nuclear pores and macromolecular assemblies involved in nucleocytoplasmic transport
    M Stewart
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Curr Opin Struct Biol 6:162-5. 1996
    ..In addition to NPC-based complexes, such as those based on nucleoporins Nsp1p and p62, cytoplasmic protein macromolecular assemblies are also important for nucleocytoplasmic transport...
  10. pmc Structural basis for the interaction between yeast Spt-Ada-Gcn5 acetyltransferase (SAGA) complex components Sgf11 and Sus1
    Andrew M Ellisdon
    From the Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom and
    J Biol Chem 285:3850-6. 2010
    ..Biol. Chem. 284, 12049-12056). A single Sus1 molecule cannot bind Sgf11 and Sac3 simultaneously and this, combined with the structure of the Sus1-Sgf11 complex, indicates that Sus1 forms separate subcomplexes within SAGA and TREX-2...
  11. pmc Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex
    Andrew M Ellisdon
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    Nat Struct Mol Biol 19:328-36. 2012
    ....
  12. pmc Structure of the N-terminal Mlp1-binding domain of the Saccharomyces cerevisiae mRNA-binding protein, Nab2
    Richard P Grant
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    J Mol Biol 376:1048-59. 2008
    ....
  13. pmc Structural basis for the function of the Saccharomyces cerevisiae Gfd1 protein in mRNA nuclear export
    Chao Zheng
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom
    J Biol Chem 285:20704-15. 2010
    ..These data support the importance of the Gfd1/Nab2 interaction for Dbp5 activity and provide further molecular details of the interactions that facilitate Dbp5-mediated mRNP remodeling in the terminal step of mRNA export...
  14. ncbi request reprint Structural basis for the assembly of a nuclear export complex
    Yoshiyuki Matsuura
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 432:872-7. 2004
    ....
  15. pmc Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export
    Divyang Jani
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nucleic Acids Res 40:4562-73. 2012
    ..Thus, we implicate TREX-2 as an integral component of the mammalian mRNA export machinery where it links transcription and nuclear export by facilitating the transfer of mature mRNPs from the nuclear interior to NPCs...
  16. pmc Structural basis for polyadenosine-RNA binding by Nab2 Zn fingers and its function in mRNA nuclear export
    Christoph Brockmann
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Structure 20:1007-18. 2012
    ....
  17. ncbi request reprint Solution NMR study of the interaction between NTF2 and nucleoporin FxFG repeats
    Jonathan Morrison
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 333:587-603. 2003
    ....
  18. ncbi request reprint Structural basis for the high-affinity binding of nucleoporin Nup1p to the Saccharomyces cerevisiae importin-beta homologue, Kap95p
    Sai Man Liu
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 349:515-25. 2005
    ..The length and composition of this linker is crucial and suggests how differences in affinity for Kap95p both between and within FG-nucleoporins arise...
  19. ncbi request reprint Structural basis for nuclear import complex dissociation by RanGTP
    Soo Jae Lee
    MRC Laboratory of Molecular Biology, Hills Rd, Cambridge CB2 2QH, UK
    Nature 435:693-6. 2005
    ..This interaction produces a change in helicoidal pitch that locks Kap95p in a conformation that cannot bind importin-alpha or cargo. We suggest an allosteric mechanism for nuclear import complex disassembly by RanGTP...
  20. pmc Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats
    Richard Bayliss
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 21:2843-53. 2002
    ....
  21. ncbi request reprint Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking
    R Bayliss
    MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
    Cell 102:99-108. 2000
    ..Instead, RanGTP may release importin-beta from FxFG nucleoporins by generating a conformational change that alters the structure of the FxFG binding site...
  22. pmc Structural basis for the molecular recognition of polyadenosine RNA by Nab2 Zn fingers
    Sonja I Kuhlmann
    MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK
    Nucleic Acids Res 42:672-80. 2014
    ....
  23. pmc Sus1, Cdc31, and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export
    Divyang Jani
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Mol Cell 33:727-37. 2009
    ..These data indicate Sac3(CID) provides a scaffold within TREX-2 to integrate interactions between protein complexes to facilitate the coupling of transcription and mRNA export during gene expression...
  24. ncbi request reprint Structural basis for the interaction between the Tap/NXF1 UBA domain and FG nucleoporins at 1A resolution
    Richard P Grant
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 326:849-58. 2003
    ....
  25. pmc Structural basis for Nup2p function in cargo release and karyopherin recycling in nuclear import
    Yoshiyuki Matsuura
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 22:5358-69. 2003
    ..These data indicate that Nup2p increases the overall rate of nuclear trafficking by coordinating nuclear import termination and importin recycling as a concerted process...
  26. pmc Novel binding of the mitotic regulator TPX2 (target protein for Xenopus kinesin-like protein 2) to importin-alpha
    Astrid Giesecke
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom
    J Biol Chem 285:17628-35. 2010
    ..The different way in which TPX2 binds to importin-alpha could account for much of the selectivity necessary during mitosis because this would reduce the competition for binding to importin-alpha from other NLS-containing proteins...
  27. pmc Nup50/Npap60 function in nuclear protein import complex disassembly and importin recycling
    Yoshiyuki Matsuura
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 24:3681-9. 2005
    ..These results provide a rationale for understanding the series of interactions that orchestrate the terminal steps of nuclear protein import...
  28. ncbi request reprint GLFG and FxFG nucleoporins bind to overlapping sites on importin-beta
    Richard Bayliss
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 277:50597-606. 2002
    ..The binding of GLFG and FxFG repeats to overlapping sites on importin-beta indicates that functional differences between different repeats probably arise from differences in their spatial organization...
  29. ncbi request reprint Molecular interactions between the importin alpha/beta heterodimer and proteins involved in vertebrate nuclear protein import
    P Percipalle
    MRC Laboratory of Molecular Biology, Cambridge, England
    J Mol Biol 266:722-32. 1997
    ..Our results are consistent with models for nuclear protein import in which Ran nucleotide exchange modulates the binding of the importin-substrate complexes during translocation through nuclear pore complexes...
  30. ncbi request reprint Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran
    W D Clarkson
    MRC Laboratory of Molecular Biology, Cambridge, UK
    J Mol Biol 272:716-30. 1997
    ..We conclude that interaction between NTF2 and GDP-Ran is important for efficient nuclear protein import...
  31. doi request reprint Structural basis for the assembly and disassembly of mRNA nuclear export complexes
    Eugene Valkov
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK
    Biochim Biophys Acta 1819:578-92. 2012
    ..This article is part of a Special Issue entitled: Nuclear Transport and RNA Processing...
  32. ncbi request reprint The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2)
    T L Bullock
    MRC Laboratory of Molecular Biology, Cambridge, UK
    J Mol Biol 260:422-31. 1996
    ....
  33. ncbi request reprint Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran
    M Stewart
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England
    J Mol Biol 277:635-46. 1998
    ..The binding of GDP-Ran at the NTF2 cavity raises the possibility that this interaction might be modulated by a metabolite or small molecule substrate for NTF2's putative enzymatic activity...
  34. pmc GANP enhances the efficiency of mRNA nuclear export in mammalian cells
    Vihandha O Wickramasinghe
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge, UK
    Nucleus 1:393-6. 2010
    ..Here, we discuss the model for GANP function presented in our recent paper and its implications for the mechanism of mRNA export in mammalian cells...
  35. ncbi request reprint Structure of MFP2 and its function in enhancing MSP polymerization in Ascaris sperm amoeboid motility
    Richard P Grant
    MRC Laboratory of Molecular Biology, Hills Rd, Cambridge CB2 2QH, UK
    J Mol Biol 347:583-95. 2005
    ..This observation, together with the structural data, suggests that MFP2 may function in a manner analogous to formins in actin-based motility...
  36. pmc mRNA export from mammalian cell nuclei is dependent on GANP
    Vihandha O Wickramasinghe
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK
    Curr Biol 20:25-31. 2010
    ..These results establish GANP as an integral component of the mammalian mRNA export machinery and suggest a model whereby GANP facilitates the transfer of NXF1-containing mRNPs to NPCs...
  37. doi request reprint Structural basis for the nuclear import of the human androgen receptor
    Mark L Cutress
    Uro Oncology Research Group, Cancer Research UK Cambridge Research Institute, Robinson Way, Cambridge, CB2 0RE, UK
    J Cell Sci 121:957-68. 2008
    ..Thus, in addition to its function in the nuclear import of AR, the NLS in the hinge region of AR has a separate, quite distinct role on transactivation, which becomes apparent once nuclear import has been achieved...
  38. ncbi request reprint NTF2 monomer-dimer equilibrium
    C Chaillan-Huntington
    Laboratory of Molecular Biology, MRC, Hills Road, Cambridge, CB2 2QH, England
    J Mol Biol 314:465-77. 2001
    ..Overall, our results indicate that dimer formation is important for NTF2 function and give insight into the formation of heterodimers by mRNA export factors such as TAP1 and NXT1 that contain NTF2-homology domains...
  39. ncbi request reprint Structure of the C-terminal FG-nucleoporin binding domain of Tap/NXF1
    Richard P Grant
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Struct Biol 9:247-51. 2002
    ..F617A Tap suppresses FG-nucleoporin binding by the most C-terminal domain that, together with the structure of the other modules from which Tap is constructed, provides a structural context for its nuclear shuttling function...
  40. pmc Electrostatic interactions involving the extreme C terminus of nuclear export factor CRM1 modulate its affinity for cargo
    Abigail M Fox
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    J Biol Chem 286:29325-35. 2011
    ....
  41. ncbi request reprint Interaction between Ran and Mog1 is required for efficient nuclear protein import
    R P Baker
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 276:41255-62. 2001
    ..Our results indicate that a primary function of Mog1 requires binding to Ran and that the Mog1-Ran interaction is necessary for efficient nuclear protein import in vivo...
  42. ncbi request reprint Cytoskeleton dynamics powers nematode sperm motility
    Murray Stewart
    MRC Laboratory of Molecular Biology, Hills Rd, Cambridge CB2 2QH, England
    Adv Protein Chem 71:383-99. 2005
    ..Overall, nematode sperm motility illustrates that cell locomotion can be generated by cytoskeletal dynamics alone without the use of myosin-like motor proteins...
  43. ncbi request reprint Crystallization and preliminary X-ray diffraction analysis of nuclear transport factor 2
    H M Kent
    MRC Laboratory of Molecular Biology, Cambridge, England
    J Struct Biol 116:326-9. 1996
    ..9 A; b = 56.7 A; c = 88.3 A and diffract past 2 A on laboratory X-ray sources. The asymmetric unit of these crystals contains two NTF2 polypeptide chains, consistent with the reported dimeric structure of the molecule...
  44. ncbi request reprint The structure of the Q69L mutant of GDP-Ran shows a major conformational change in the switch II loop that accounts for its failure to bind nuclear transport factor 2 (NTF2)
    M Stewart
    MRC Laboratory of Molecular Biology, Hills Rd, Cambridge, CB2 2QH
    J Mol Biol 284:1517-27. 1998
    ..This interaction between NTF2 and GDP-Ran is required for efficient nuclear protein import and may function between the docking and translocation steps of the pathway...
  45. ncbi request reprint 2.6 A resolution crystal structure of helices of the motile major sperm protein (MSP) of Caenorhabditis elegans
    Anne M E Baker
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 319:491-9. 2002
    ..The interfaces frequently involve the loop containing residues 78-85, which is divergent between MSP homologues, and the loop containing residues 98-103, which is highly conserved...
  46. ncbi request reprint Crystallization and preliminary X-ray diffraction analysis of the Saccharomyces cerevisiae ran-binding protein Mog1p
    R P Baker
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, England
    Acta Crystallogr D Biol Crystallogr 56:229-31. 2000
    ..The crystals have P2(1)2(1)2(1) symmetry, with unit-cell parameters a = 39.67, b = 51.96, c = 112.23 A, a Matthews coefficient of 2.44 A(3) Da(-1), an estimated solvent content of 49.5% and one chain in the asymmetric unit...
  47. pmc Macromolecular interactions in the nucleoporin p62 complex of rat nuclear pores: binding of nucleoporin p54 to the rod domain of p62
    F Buss
    Medical Research Council Laboratory of Molecular Biology, Cambridge, England
    J Cell Biol 128:251-61. 1995
    ..This model is consistent with data that indicate that the analogous coiled-coil region of yeast nucleoporin NSP1 may function in a similar way...
  48. ncbi request reprint Structural basis for vertebrate filamin dimerization
    Regina Pudas
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Finland
    Structure 13:111-9. 2005
    ..Furthermore, we show that point mutations in the dimerization interface disrupt the dimer and that the dissociation constant for recombinant Ig24 is in the micromolar range...
  49. ncbi request reprint The molecular mechanism of translocation through the nuclear pore complex is highly conserved
    Carl Feldherr
    Department of Anatomy and Cell Biology, University of Florida, College of Medicine, Gainesville 32610, USA
    J Cell Sci 115:2997-3005. 2002
    ..Since selected nuclear import pathways can be investigated in the absence of competing endogenous transport, A. proteus might provide a useful in vivo system for investigating specific molecular interactions involved in trafficking...
  50. pmc Coordination of Hpr1 and ubiquitin binding by the UBA domain of the mRNA export factor Mex67
    Maria Hobeika
    Institut Jacques Monod, Universités Paris VI and VII, Centre National de la Recherche Scientifique, 75251 Paris Cedex 05, France
    Mol Biol Cell 18:2561-8. 2007
    ....
  51. ncbi request reprint Meeting report: International Symposium on 'Ran and the Cell Cycle', October 2-5, 2005, Awaji Yumebutai, Japan
    Mary Dasso
    Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, MD 20892 5431, USA
    Traffic 7:474-8. 2006
  52. ncbi request reprint Karyopherin flexibility in nucleocytoplasmic transport
    Elena Conti
    EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Curr Opin Struct Biol 16:237-44. 2006
    ..Flexibility appears to be an intrinsic feature of such superhelices and might be functionally important not only for karyopherins and nuclear transport, but also for HEAT repeat proteins from other biological systems...
  53. doi request reprint Kap95p binding induces the switch loops of RanGDP to adopt the GTP-bound conformation: implications for nuclear import complex assembly dynamics
    Jade K Forwood
    School of Biomedical Sciences, Charles Sturt University, Wagga Wagga 2650, Australia
    J Mol Biol 383:772-82. 2008
    ..The structure of the complex provides insights into the structural basis for the gradation of affinities regulating nuclear protein transport...
  54. ncbi request reprint Classical nuclear localization signals: definition, function, and interaction with importin alpha
    Allison Lange
    Department of Biochemistry, School of Medicine, Emory University, Atlanta, Georgia 30322, USA
    J Biol Chem 282:5101-5. 2007
    ..Finally, we describe how a predicted NLS within a protein of interest can be confirmed experimentally to be functionally important...
  55. pmc Structural basis for tropomyosin overlap in thin (actin) filaments and the generation of a molecular swivel by troponin-T
    Kenji Murakami
    Department of Biosciences, School of Science and Engineering, Teikyo University, Toyosatodai 1 1, Utsunomiya 320 8551, Japan
    Proc Natl Acad Sci U S A 105:7200-5. 2008
    ..On actin filaments, the head-to-tail junction can function as a molecular swivel to accommodate irregularities in the coiled-coil path between successive tropomyosins enabling each to interact equivalently with the actin helix...
  56. pmc Functional significance of the interaction between the mRNA-binding protein, Nab2, and the nuclear pore-associated protein, Mlp1, in mRNA export
    Milo B Fasken
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 283:27130-43. 2008
    ..These data provide in vivo evidence for a model of mRNA export in which Nab2 is important for targeting mRNAs to the nuclear pore for export...