Graham R Stewart
Affiliation: University of Surrey
- GSMN-TB: a web-based genome-scale network model of Mycobacterium tuberculosis metabolismDany J V Beste
School of Biomedical and Molecular Sciences, University of Surrey, Stag Hill, Guildford, Surrey, GU2 7XH, UK
Genome Biol 8:R89. 2007..Constraint-based modeling provides a novel approach to investigating microbial metabolism but has not yet been applied to genome-scale modeling of M. tuberculosis...
- Genetic determination of the effect of post-translational modification on the innate immune response to the 19 kDa lipoprotein of Mycobacterium tuberculosisKatalin A Wilkinson
National Institute for Medical Research, Mill Hill, London, UK
BMC Microbiol 9:93. 2009..We also reintroduced the 19 kDa gene in two modified forms that lacked motifs for acylation (Delta19::19NA) and O-glycosylation (Delta19::19NOG)...
- The stress-responsive chaperone alpha-crystallin 2 is required for pathogenesis of Mycobacterium tuberculosisGraham R Stewart
Department of Infectious Diseases and Microbiology, Centre for Molecular Microbiology and Infection, Imperial College London, South Kensington, London SW7 2AZ, UK
Mol Microbiol 55:1127-37. 2005..These findings demonstrate that both alpha-crystallins contribute to persistent infection with M. tuberculosis and suggest that manipulation of acr expression can influence the host response to infection...
- Infection biology of a novel alpha-crystallin of Mycobacterium tuberculosis: Acr2Katalin A Wilkinson
Wellcome Trust Center for Research in Clinical Tropical Medicine and Department of Pediatric Infectious Diseases, Faculty of Medicine, Imperial College London, Wright Fleming Institute, UK
J Immunol 174:4237-43. 2005..The demonstration that there is infection stage-specific immunity to tuberculosis has implications for vaccine design...
- Effect of deletion or overexpression of the 19-kilodalton lipoprotein Rv3763 on the innate response to Mycobacterium tuberculosisGraham R Stewart
Center for Molecular Microbiology and Infection and Wellcome Trust Center for Research in Clinical Tropical Medicine, Imperial College London, UK
Infect Immun 73:6831-7. 2005..tuberculosis with phagocytes. However, this analysis indicates that in the context of the whole bacillus, the 19-kDa lipoprotein is only one of a number of molecules that mediate the innate response to M. tuberculosis...
- A role for dendritic cells in the dissemination of mycobacterial infectionIan R Humphreys
Department of Infectious Diseases and Microbiology, Centre for Molecular Microbiology and Infection, Flowers Building, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
Microbes Infect 8:1339-46. 2006..The results are consistent with a model in which mycobacterial dissemination from the lung is initiated by the migration of infected dendritic cells to the draining lymph nodes...
- The OtsAB pathway is essential for trehalose biosynthesis in Mycobacterium tuberculosisHelen N Murphy
Centre for Molecular Microbiology and Infection, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
J Biol Chem 280:14524-9. 2005....
- Tetracycline-inducible gene regulation in mycobacteriaMarian C J Blokpoel
Department of Infectious Diseases and Microbiology, Centre for Molecular Microbiology and Infection, Imperial College London South Kensington campus, London SW7 2AZ, UK
Nucleic Acids Res 33:e22. 2005..These results demonstrate the potential of this tetracycline-regulated system for the manipulation of mycobacterial gene expression inside and outside cells...
- Analysis of the function of mycobacterial DnaJ proteins by overexpression and microarray profilingGraham R Stewart
Centre for Molecular Microbiology and Infection, Flowers Building, South Kensington Campus, Imperial College London, SW7 2AZ, UK
Tuberculosis (Edinb) 84:180-7. 2004..Overexpression in combination with microarray profiling provides a complementary approach to gene deletion for exploring the function of essential genes in M. tuberculosis...
- Visualization of microarray results to assist interpretationIrene Papatheodorou
Department of Computing, Imperial College London, SW7 2AZ, UK
Tuberculosis (Edinb) 84:275-81. 2004..The GeneGraph project can be accessed at: zebrafish.doc.ic.ac.uk..
- Tuberculosis: a problem with persistenceGraham R Stewart
Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK
Nat Rev Microbiol 1:97-105. 2003..tuberculosis dates back to the first half of the last century. Recent advances in microbial genetics and host immunity provide an opportunity for renewed investigation of this persistent threat to human health...
- Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarraysGraham R Stewart
Department of Infectious Diseases and Microbiology, Centre for Molecular Microbiology and Infection, Imperial College of Science Technology and Medicine, London SW7 2AZ, UK
Microbiology 148:3129-38. 2002..This protein is one of the most prominent features of the M. tuberculosis heat-shock response and is related to a major antigen induced in response to anaerobic stress...
- Tuberculosis vaccinesDouglas B Young
Centre for Molecular Microbiology and Infection, Faculty of Medicine, Imperial College of Science, Technology and Medicine, South Kensington, London, UK
Br Med Bull 62:73-86. 2002..Evaluation of the immunogenicity of selected candidate vaccines in clinical trials should be combined with a continuation of fundamental research on the immune response to mycobacterial infection and persistence...
- Heat-shock proteins and the host-pathogen interaction during bacterial infectionGraham R Stewart
Department of Infectious Diseases and Microbiology, Centre for Molecular Microbiology and Infection, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
Curr Opin Immunol 16:506-10. 2004..Manipulation of these interactions presents a potential route to improved control of infection by vaccination or immunotherapy...
- A deletion defining a common Asian lineage of Mycobacterium tuberculosis associates with immune subversionSandra M Newton
Wellcome Trust Center for Research in Clinical Tropical Medicine, Center for Molecular Microbiology and Infection, and Kennedy Institute of Rheumatology, Faculty of Medicine, Imperial College London, London W2 1PG, United Kingdom
Proc Natl Acad Sci U S A 103:15594-8. 2006..The Rv1519 polymorphism in M. tuberculosis confers an immune subverting phenotype that contributes to the persistence and outbreak potential of this lineage...
- A mutant of Mycobacterium tuberculosis lacking the 19-kDa lipoprotein Rv3763 is highly attenuated in vivo but retains potent vaccinogenic propertiesMarcela Henao-Tamayo
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80524, USA
Vaccine 25:7153-9. 2007..These data show that despite being highly attenuated, the Delta 19 mutant strongly retained vaccinogenic properties...
- Induction of apoptosis and release of interleukin-1 beta by cell wall-associated 19-kDa lipoprotein during the course of mycobacterial infectionAntonio Ciaramella
Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
J Infect Dis 190:1167-76. 2004..These findings indicate that the 19-kDa lipoprotein is the main signal required to trigger both apoptosis and the release of IL-1 beta during the early stages of mycobacterial infection...
- Deciphering the molecular bases of Mycobacterium tuberculosis binding to the lectin DC-SIGN reveals an underestimated complexitySylvain Pitarque
Institut de Pharmacologie et de Biologie Structurale, Département Mécanismes Moléculaires des Infections Mycobactériennes, CNRS UMR 5089, 205 route de Narbonne, 31077 Toulouse Cedex 4, France
Biochem J 392:615-24. 2005..tuberculosis binding to DC-SIGN is more complicated than previously thought and provides further insight into the mechanisms of M. tuberculosis recognition by the immune system...