David J Stephens

Summary

Affiliation: University of Bristol
Country: UK

Publications

  1. pmc Coupling of ER exit to microtubules through direct interaction of COPII with dynactin
    Peter Watson
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Nat Cell Biol 7:48-55. 2005
  2. doi request reprint Functional coupling of microtubules to membranes - implications for membrane structure and dynamics
    David J Stephens
    Cell Biology Laboratories, School of Biochemistry, University of Bristol, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 125:2795-804. 2012
  3. ncbi request reprint Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells
    David J Stephens
    Cell Biology and Cell Biophysics Programme, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Cell Sci 115:1149-60. 2002
  4. pmc De novo formation, fusion and fission of mammalian COPII-coated endoplasmic reticulum exit sites
    David J Stephens
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    EMBO Rep 4:210-7. 2003
  5. ncbi request reprint Meeting report--Imaging membrane dynamics, 14-17 September, Royal Holloway--University of London, UK
    David J Stephens
    Department of Biochemistry, University of Bristol School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
    Traffic 8:448-50. 2007
  6. ncbi request reprint Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum
    David J Stephens
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 117:3635-44. 2004
  7. pmc Organisation of human ER-exit sites: requirements for the localisation of Sec16 to transitional ER
    Helen Hughes
    Cell Biology Laboratories, Department of Biochemistry, University of Bristol School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 122:2924-34. 2009
  8. pmc Specificity of cytoplasmic dynein subunits in discrete membrane-trafficking steps
    Krysten J Palmer
    Cell Biology Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS81TD, United Kingdom
    Mol Biol Cell 20:2885-99. 2009
  9. pmc The retromer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network
    Thomas Wassmer
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, UK
    Dev Cell 17:110-22. 2009
  10. ncbi request reprint SNX4 coordinates endosomal sorting of TfnR with dynein-mediated transport into the endocytic recycling compartment
    Colin J Traer
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Nat Cell Biol 9:1370-80. 2007

Collaborators

Detail Information

Publications43

  1. pmc Coupling of ER exit to microtubules through direct interaction of COPII with dynactin
    Peter Watson
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Nat Cell Biol 7:48-55. 2005
    ..Together, our data suggest a mechanism by which membranes of the early secretory pathway can be linked to motors and microtubules for subsequent organization and movement to the Golgi apparatus...
  2. doi request reprint Functional coupling of microtubules to membranes - implications for membrane structure and dynamics
    David J Stephens
    Cell Biology Laboratories, School of Biochemistry, University of Bristol, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 125:2795-804. 2012
    ....
  3. ncbi request reprint Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells
    David J Stephens
    Cell Biology and Cell Biophysics Programme, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Cell Sci 115:1149-60. 2002
    ..These data reveal the existence of an early COPI-dependent, pre-Golgi cargo sorting step in mammalian cells...
  4. pmc De novo formation, fusion and fission of mammalian COPII-coated endoplasmic reticulum exit sites
    David J Stephens
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    EMBO Rep 4:210-7. 2003
    ..These three mechanisms of de novo formation, fusion and fission probably cooperate to regulate the size of these sites in mammalian cells...
  5. ncbi request reprint Meeting report--Imaging membrane dynamics, 14-17 September, Royal Holloway--University of London, UK
    David J Stephens
    Department of Biochemistry, University of Bristol School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
    Traffic 8:448-50. 2007
  6. ncbi request reprint Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum
    David J Stephens
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 117:3635-44. 2004
    ..These data show a differential requirement for efficient GTP hydrolysis by the Sar1p GTPase in export of cargo from the ER...
  7. pmc Organisation of human ER-exit sites: requirements for the localisation of Sec16 to transitional ER
    Helen Hughes
    Cell Biology Laboratories, Department of Biochemistry, University of Bristol School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 122:2924-34. 2009
    ..The central conserved domain of Sec16 binds to Sec13 linking tER membrane localisation with COPII vesicle formation. These data are consistent with a model where Sec16 acts as a platform for COPII assembly at ERES...
  8. pmc Specificity of cytoplasmic dynein subunits in discrete membrane-trafficking steps
    Krysten J Palmer
    Cell Biology Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS81TD, United Kingdom
    Mol Biol Cell 20:2885-99. 2009
    ....
  9. pmc The retromer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network
    Thomas Wassmer
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, UK
    Dev Cell 17:110-22. 2009
    ..These interactions describe fundamental steps in retromer-mediated transport and establish that the spatial organization of the retromer network is a critical element required for efficient retromer-mediated sorting...
  10. ncbi request reprint SNX4 coordinates endosomal sorting of TfnR with dynein-mediated transport into the endocytic recycling compartment
    Colin J Traer
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Nat Cell Biol 9:1370-80. 2007
    ..Finally, these data suggest that by associating with molecular motors, SNX-BAR proteins may coordinate sorting with carrier transport between donor and recipient membranes...
  11. pmc Microtubule plus-end loading of p150(Glued) is mediated by EB1 and CLIP-170 but is not required for intracellular membrane traffic in mammalian cells
    Peter Watson
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
    J Cell Sci 119:2758-67. 2006
    ..A subset of these structures colocalizes with ER-Golgi transport intermediates. Together, these data show that the function of CLIP-170 and p150(Glued) in membrane trafficking is not associated with their plus-end localization...
  12. pmc Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion
    Anna K Townley
    Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol, BS8 1TD, UK
    J Cell Sci 125:673-84. 2012
    ..Our results provide insight into the role of COPII in epithelial morphogenesis and have implications for the interpretation of epithelial polarity and organization assays in cell culture...
  13. pmc Optimising the precision for localising fluorescent proteins in living cells by 2D Gaussian fitting of digital images: application to COPII-coated endoplasmic reticulum exit sites
    Peter Spence
    School of Chemistry, Cantock s Close, University of Bristol, Bristol, BS8 1TS, UK
    Eur Biophys J 37:1335-49. 2008
    ....
  14. pmc Sec16 defines endoplasmic reticulum exit sites and is required for secretory cargo export in mammalian cells
    Peter Watson
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Traffic 7:1678-87. 2006
    ..Our data suggest that Sar1-GTP-dependent assembly of Sec16 on the ER membrane forms an organized scaffold defining an ERES...
  15. ncbi request reprint Secretory cargo regulates the turnover of COPII subunits at single ER exit sites
    Rebecca Forster
    Cell Biology and Biophysics Unit, EMBL, 69117 Heidelberg, Germany
    Curr Biol 16:173-9. 2006
    ..We conclude that secretory cargo retains the COPII complex on membranes, after Sar1p release has occurred, and prevents premature disassembly of COPII during cargo sorting and transport carrier formation...
  16. pmc Sec16A defines the site for vesicle budding from the endoplasmic reticulum on exit from mitosis
    Helen Hughes
    Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol, BS8 1TD, UK
    J Cell Sci 123:4032-8. 2010
    ..Full assembly of COPII at exit sites precedes reassembly of the Golgi in telophase...
  17. pmc Specific functions of BIG1 and BIG2 in endomembrane organization
    Frédéric Boal
    Department of Biochemistry, Cell Biology Laboratories, University of Bristol School of Medical Sciences, Bristol, United Kingdom
    PLoS ONE 5:e9898. 2010
    ..Among the human BFA-sensitive large Arf-GEFs, the function of the two closely related BIG1 and BIG2 is still not clear, and recent studies have raised the question of functional redundancy between the two proteins...
  18. doi request reprint Efficient coupling of Sec23-Sec24 to Sec13-Sec31 drives COPII-dependent collagen secretion and is essential for normal craniofacial development
    Anna K Townley
    Department of Biochemistry, University of Bristol School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 121:3025-34. 2008
    ....
  19. pmc Characterization of human Sec16B: indications of specialized, non-redundant functions
    Annika Budnik
    Cell Biology Laboratories, School of Biochemistry, University of Bristol, Medical Sciences Building, University Walk, Bristol, BS8 1TD
    Sci Rep 1:77. 2011
    ..Here we describe characterization of the localization and dynamics of Sec16B relative to Sec16A, provide evidence that Sec16B is likely a minor or perhaps specialized form of Sec16, and that it is not functionally redundant with Sec16A...
  20. doi request reprint The role of motor proteins in endosomal sorting
    Sylvie D Hunt
    Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK
    Biochem Soc Trans 39:1179-84. 2011
    ....
  21. doi request reprint Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport
    Vijay Gupta
    Cell Biology Laboratories, Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol, BS81TD, UK
    Traffic 9:1850-66. 2008
    ..These data implicate kinesin-1 in the spatial organization of the ER/Golgi interface as well as in traffic outside the ER...
  22. ncbi request reprint ER-to-Golgi transport: form and formation of vesicular and tubular carriers
    Peter Watson
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Biochim Biophys Acta 1744:304-15. 2005
    ..Here, we seek to integrate much of the data surrounding this topic and try to understand the mechanisms by which vesicular and/or tubular carriers might be generated...
  23. ncbi request reprint PCTAIRE protein kinases interact directly with the COPII complex and modulate secretory cargo transport
    Krysten J Palmer
    Department of Biochemistry, University of Bristol, School of Medical Science, University Walk, Bristol, BS8 1TD, UK
    J Cell Sci 118:3839-47. 2005
    ..These data show a role for PCTAIRE protein kinase function in membrane traffic through the early secretory pathway...
  24. ncbi request reprint Biogenesis of ER-to-Golgi transport carriers: complex roles of COPII in ER export
    Krysten J Palmer
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol, UK BS8 1TD
    Trends Cell Biol 14:57-61. 2004
    ..Here, we examine the evidence for this controversial hypothesis...
  25. pmc Assembly, organization, and function of the COPII coat
    Helen Hughes
    Cell Biology Laboratories, Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Histochem Cell Biol 129:129-51. 2008
    ..A significant outcome of such a full understanding is to reveal how the machinery and processes of membrane trafficking through the early secretory pathway fail in disease states...
  26. doi request reprint LG186: An inhibitor of GBF1 function that causes Golgi disassembly in human and canine cells
    Frédéric Boal
    Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol BS8 1TD, UK
    Traffic 11:1537-51. 2010
    ..Unlike other Arf-GEF and reported GBF1 inhibitors including BFA, Exo2 and Golgicide A, LG186 induces disassembly of the Golgi stack in both human and canine cells...
  27. pmc Early stages of retinal development depend on Sec13 function
    Katy Schmidt
    Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol BS8 1TD, UK Present address Max F Perutz Laboratories, University of Vienna and Medical University of Vienna, Dr Bohr Gasse 9 3, 1030 Wien, Austria
    Biol Open 2:256-66. 2013
    ..Our data show that the outer layer of the COPII coat is also necessary for the transport of large amounts of cargo proteins, in this case rhodopsin, rather than just large cargo as previously thought...
  28. pmc Microtubule motors mediate endosomal sorting by maintaining functional domain organization
    Sylvie D Hunt
    Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 126:2493-501. 2013
    ..Taken together, our data show that these key features of endosome dynamics are governed by the same set of opposing microtubule motors. Thus, microtubule motors help to define the mosaic layout of endosomes that underpins cargo sorting...
  29. ncbi request reprint Light microscopy techniques for live cell imaging
    David J Stephens
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
    Science 300:82-6. 2003
    ..For anyone new to this area, however, it can be daunting to decide which techniques or equipment to try. Here, we aim to give a brief overview of the main approaches to live cell imaging, with some mention of their pros and cons...
  30. ncbi request reprint Intracellular trafficking pathways and drug delivery: fluorescence imaging of living and fixed cells
    Peter Watson
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
    Adv Drug Deliv Rev 57:43-61. 2005
    ..In particular, we shall focus on the application of live cell imaging to the study of endocytic drug delivery...
  31. ncbi request reprint Measuring the induction or inhibition of apoptosis by HPV proteins
    Anna M Kowalczyk
    Department of Biochemistry, School of Medical Sciences, University of Bristol, UK
    Methods Mol Med 119:419-32. 2005
    ..In other cases, viral proteins inhibit apoptosis. In this chapter, we will describe some of the methods that can be used to investigate the induction or inhibition of apoptosis by papillomavirus proteins...
  32. pmc A role for Tctex-1 (DYNLT1) in controlling primary cilium length
    Krysten J Palmer
    Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol BS8 1TD, UK
    Eur J Cell Biol 90:865-71. 2011
    ..Compared to individual depletions, double siRNA depletion of DHC2 and Tctex-1 causes an even greater increase in cilia length. Our data show that Tctex-1 is a key regulator of cilia length and most likely functions as part of dynein-2...
  33. doi request reprint Cargo loading at the ER
    Katy Schmidt
    Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, Bristol, UK
    Mol Membr Biol 27:398-411. 2010
    ..Combined, these approaches are now starting to shed light not only on the mechanisms of macromolecular cargo export from the ER but also reveal the implications of failure of this process to human development and disease...
  34. doi request reprint ER exit sites--localization and control of COPII vesicle formation
    Annika Budnik
    Cell Biology Laboratories, Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    FEBS Lett 583:3796-803. 2009
    ....
  35. ncbi request reprint A role for glycogen synthase kinase-3 in mitotic spindle dynamics and chromosome alignment
    James G Wakefield
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    J Cell Sci 116:637-46. 2003
    ..We propose that GSK-3 is regulated in a temporal and spatial manner during mitosis and, through controlling microtubule dynamics, plays an important role in chromosomal alignment on the metaphase plate...
  36. pmc Bap31 is an itinerant protein that moves between the peripheral endoplasmic reticulum (ER) and a juxtanuclear compartment related to ER-associated Degradation
    Yuichi Wakana
    School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Mol Biol Cell 19:1825-36. 2008
    ..Overexpression of Sar1p and Arf1 mutants affected Bap31 cycling, suggesting that this cycling pathway is related to the conventional vesicular transport pathways...
  37. pmc The secretion inhibitor Exo2 perturbs trafficking of Shiga toxin between endosomes and the trans-Golgi network
    Robert A Spooner
    Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK
    Biochem J 414:471-84. 2008
    ....
  38. ncbi request reprint Analysis of GTPase-activating proteins: Rab1 and Rab43 are key Rabs required to maintain a functional Golgi complex in human cells
    Alexander K Haas
    Cancer Research Centre, University of Liverpool, 200 London Road, Liverpool, L9 3AT, UK
    J Cell Sci 120:2997-3010. 2007
    ....
  39. ncbi request reprint The intracellular transport of chylomicrons requires the small GTPase, Sar1b
    Carol C Shoulders
    Medical Research Council Clinical Sciences Centre, Hammersmith Hospital, London, UK
    Curr Opin Lipidol 15:191-7. 2004
    ..This review focuses on the assembly and structural evolution of COPII (coat protein) transport carriers that are essential for the transport of chylomicrons from the endoplasmic reticulum to the Golgi apparatus...
  40. ncbi request reprint Nordihydroguaiaretic acid affects multiple dynein-dynactin functions in interphase and mitotic cells
    Kohei Arasaki
    School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Mol Pharmacol 71:454-60. 2007
    ..To our knowledge, NDGA is the first case of a reagent that can modulate dynein-dynactin-related processes...
  41. ncbi request reprint Phosphatidylinositol 4-kinase is required for endosomal trafficking and degradation of the EGF receptor
    Shane Minogue
    Centre for Molecular Cell Biology, Department of Medicine, Royal Free and University College Medical School, University College London, Rowland Hill Street, London, NW3 2PF, UK
    J Cell Sci 119:571-81. 2006
    ..We demonstrate that phosphatidylinositol 4-kinase IIalpha is necessary for the correct endocytic traffic and downregulation of activated epidermal growth factor receptor...
  42. ncbi request reprint Coming out of the dark: the evolving role of fluorescence imaging in drug delivery research
    Mark Gumbleton
    Welsh School of Pharmacy, Cardiff University, Cardiff CF14 3RH, UK
    Adv Drug Deliv Rev 57:5-15. 2005
  43. pmc Role of adaptor complex AP-3 in targeting wild-type and mutated CD63 to lysosomes
    Brian A Rous
    University of Cambridge, Department of Clinical Biochemistry, Cambridge Institute for Medical Research, Cambridge, CB2 2XY, United Kingdom
    Mol Biol Cell 13:1071-82. 2002
    ..Using this AP-3-dependent mutant of CD63, we have shown that AP-3 functions in membrane traffic from the trans-Golgi network to lysosomes via an intracellular route that appears to bypass early endosomes...