M A Stanley

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. pmc Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR
    Ian Roberts
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 0XZ, UK
    BMC Biotechnol 8:57. 2008
  2. ncbi request reprint Prognostic factors and new therapeutic approaches to cervical cancer
    Margaret A Stanley
    Department of Pathology, University of Cambridge, Tennis Court Road, UK
    Virus Res 89:241-8. 2002
  3. ncbi request reprint Progress in prophylactic and therapeutic vaccines for human papillomavirus infection
    Margaret A Stanley
    Department of Pathology, Cambridge, UK
    Expert Rev Vaccines 2:381-9. 2003
  4. doi request reprint Human papillomavirus vaccines versus cervical cancer screening
    M Stanley
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
    Clin Oncol (R Coll Radiol) 20:388-94. 2008
  5. ncbi request reprint HPV: from infection to cancer
    M A Stanley
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, U K
    Biochem Soc Trans 35:1456-60. 2007
  6. ncbi request reprint Prophylactic HPV vaccines
    Margaret Stanley
    Department of Pathology, University of Cambridge, Cambridge, UK
    Drugs Today (Barc) 43:737-44. 2007
  7. ncbi request reprint Prevention strategies against the human papillomavirus: the effectiveness of vaccination
    M Stanley
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Gynecol Oncol 107:S19-23. 2007
  8. ncbi request reprint HPV vaccines: where are we now?
    Margaret Stanley
    Department of Pathology, University of Cambridge, Cambridge, UK
    J Fam Plann Reprod Health Care 33:227-9. 2007
  9. ncbi request reprint Immune responses to human papillomavirus
    Margaret Stanley
    University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
    Vaccine 24:S16-22. 2006
  10. ncbi request reprint HPV vaccines
    Margaret Stanley
    Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
    Best Pract Res Clin Obstet Gynaecol 20:279-93. 2006

Collaborators

Detail Information

Publications46

  1. pmc Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR
    Ian Roberts
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 0XZ, UK
    BMC Biotechnol 8:57. 2008
    ..We assessed a modified method, in which external calibration curves were generated from a single construct containing HPV16 E2, HPV16 E6 and the host gene hydroxymethylbilane synthase in a 1:1:1 ratio...
  2. ncbi request reprint Prognostic factors and new therapeutic approaches to cervical cancer
    Margaret A Stanley
    Department of Pathology, University of Cambridge, Tennis Court Road, UK
    Virus Res 89:241-8. 2002
    ....
  3. ncbi request reprint Progress in prophylactic and therapeutic vaccines for human papillomavirus infection
    Margaret A Stanley
    Department of Pathology, Cambridge, UK
    Expert Rev Vaccines 2:381-9. 2003
    ..In Phase I and II trials these vaccines are immunogenic and safe but show limited efficacy...
  4. doi request reprint Human papillomavirus vaccines versus cervical cancer screening
    M Stanley
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
    Clin Oncol (R Coll Radiol) 20:388-94. 2008
    ..However, if both vaccination and screening are combined then the virtual elimination of cervical cancer and the other HPV 16 and 18-associated cancers is possible...
  5. ncbi request reprint HPV: from infection to cancer
    M A Stanley
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, U K
    Biochem Soc Trans 35:1456-60. 2007
    ....
  6. ncbi request reprint Prophylactic HPV vaccines
    Margaret Stanley
    Department of Pathology, University of Cambridge, Cambridge, UK
    Drugs Today (Barc) 43:737-44. 2007
    ..The health economic benefits will be substantial but the overwhelming benefit is the major improvement for human health and well-being. (..
  7. ncbi request reprint Prevention strategies against the human papillomavirus: the effectiveness of vaccination
    M Stanley
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Gynecol Oncol 107:S19-23. 2007
    ..Predictive data also indicate that the implementation of HPV vaccination within a national screening program is likely to be cost-effective relative to current clinical practice...
  8. ncbi request reprint HPV vaccines: where are we now?
    Margaret Stanley
    Department of Pathology, University of Cambridge, Cambridge, UK
    J Fam Plann Reprod Health Care 33:227-9. 2007
  9. ncbi request reprint Immune responses to human papillomavirus
    Margaret Stanley
    University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
    Vaccine 24:S16-22. 2006
    ..Available data suggests that these vaccines are safe, produce high levels of antibodies, and are effective at preventing HPV infection...
  10. ncbi request reprint HPV vaccines
    Margaret Stanley
    Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
    Best Pract Res Clin Obstet Gynaecol 20:279-93. 2006
    ..Therapeutic vaccines for low-grade intraepithelial disease are realistic but high-grade disease presents major hurdles for immunotherapies...
  11. pmc Prophylactic HPV vaccines: prospects for eliminating ano-genital cancer
    M Stanley
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Br J Cancer 96:1320-3. 2007
    ....
  12. doi request reprint Immunobiology of human papillomavirus infection and vaccination - implications for second generation vaccines
    Margaret Stanley
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    Vaccine 26:K62-7. 2008
    ..This will be central in the development of adjuvants and vaccine formulations that induce the response to provide effective protection...
  13. ncbi request reprint Imiquimod and the imidazoquinolones: mechanism of action and therapeutic potential
    M A Stanley
    University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
    Clin Exp Dermatol 27:571-7. 2002
    ..These compounds also have adjuvant properties that could significantly enhance conventional vaccine strategies...
  14. ncbi request reprint Human papillomavirus vaccines
    Margaret A Stanley
    Department of Pathology, Cambridge, UK
    Rev Med Virol 16:139-49. 2006
    ..Second generation vaccines could include an early antigen for protection post-exposure and alternative delivery systems may be needed for the developing world...
  15. pmc A C-terminal helicase domain of the human papillomavirus E1 protein binds E2 and the DNA polymerase alpha-primase p68 subunit
    P J Masterson
    Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    J Virol 72:7407-19. 1998
    ..The modelling closely matches the deletion analysis in suggesting that this region of E1 is indeed a structural domain, and our results suggest that it is multifunctional and critical to several stages of HPV DNA replication...
  16. ncbi request reprint Immune responses to human papilloma viruses
    M A Stanley
    Department of Pathology, University of Cambridge, Cambridge, UK
    Indian J Med Res 130:266-76. 2009
    ..These vaccines are delivered intramuscularly and therefore circumvent the immune evasion strategies of the virus...
  17. ncbi request reprint Human papillomavirus vaccines
    Margaret A Stanley
    Department of Pathology, University of Cambridge, UK
    Curr Opin Mol Ther 4:15-22. 2002
    ..Immunogenicity, but no efficacy, has been demonstrated in small clinical trials with some of these approaches...
  18. ncbi request reprint Detection of viral DNA and E4 protein in basal keratinocytes of experimental canine oral papillomavirus lesions
    P K Nicholls
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, United Kingdom
    Virology 284:82-98. 2001
    ..Detection of viral DNA in post-regression tissue indicated latent infection...
  19. ncbi request reprint The immunology of animal papillomaviruses
    P K Nicholls
    Department of Pathology, University of Cambridge, UK
    Vet Immunol Immunopathol 73:101-27. 2000
    ....
  20. ncbi request reprint Regression of canine oral papillomas is associated with infiltration of CD4+ and CD8+ lymphocytes
    P K Nicholls
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, United Kingdom
    Virology 283:31-9. 2001
    ..The lymphocyte infiltrate in the dog resembled that in human papillomavirus lesions, indicating that COPV is an appropriate model for human papillomavirus immunity...
  21. ncbi request reprint Intra-epithelial vaccination with COPV L1 DNA by particle-mediated DNA delivery protects against mucosal challenge with infectious COPV in beagle dogs
    M A Stanley
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Vaccine 19:2783-92. 2001
    ..Both cell-mediated lymphoproliferative and humoral antibody responses to the DNA vaccine were observed. Our data indicate that PMDD of plasmid DNA can protect against mucosal challenge with papillomavirus...
  22. ncbi request reprint Intraepithelial DNA immunisation with a plasmid encoding a codon optimised COPV E1 gene sequence, but not the wild-type gene sequence completely protects against mucosal challenge with infectious COPV in beagles
    Richard A Moore
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Virology 304:451-9. 2002
    ....
  23. ncbi request reprint Cell-mediated immune responses to COPV early proteins
    Suchitra Jain
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Virology 356:23-34. 2006
    ....
  24. ncbi request reprint An increase in DNA double-strand breaks, induced by Ku70 depletion, is associated with human papillomavirus 16 episome loss and de novo viral integration events
    D M Winder
    Medical Research Council Cancer Cell Unit, MRC Hutchison Research Centre, Hills Road, Cambridge, CB2 0XZ, UK
    J Pathol 213:27-34. 2007
    ..We found no evidence to support the notion that major chromosomal instability precedes HPV16 integration, although such instability is an important consequence of the integration event...
  25. ncbi request reprint Absence of canine oral papillomavirus DNA following prophylactic L1 particle-mediated immunotherapeutic delivery vaccination
    R A Moore
    Department of Pathology, Cambridge University, Cambridge CB2 1QP, UK
    J Gen Virol 83:2299-301. 2002
    ..These data support PMID of COPV L1 as a protective vaccine and suggest that PMID of L1 may induce virus clearance...
  26. ncbi request reprint Human papillomavirus and cervical carcinogenesis
    M A Stanley
    Department of Pathology, Tennis Court Road, Cambridge, CB2 1QP, UK
    Best Pract Res Clin Obstet Gynaecol 15:663-76. 2001
    ..These are rare events but viral persistence and chronic exposure to steroid hormones increase the probability of this deregulation...
  27. pmc HPV & head and neck cancer: a descriptive update
    Peter K C Goon
    Department of Pathology, University of Cambridge, Cambridge, UK
    Head Neck Oncol 1:36. 2009
    ..Finally, the improved prognosis and treatment responses to chemotherapy and radiotherapy by HPV+ tumours may suggest that HPV status detection is required to better plan and individualize patient treatment regimes...
  28. pmc Sensitive HPV detection in oropharyngeal cancers
    David M Winder
    Dept of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    BMC Cancer 9:440. 2009
    ..To help ascertain a useful PCR detection protocol for oropharyngeal cancers, we directly compared three commonly used primer sets in detection of HPV from different clinical samples...
  29. ncbi request reprint Therapeutic immunisation with COPV early genes by epithelial DNA delivery
    Richard A Moore
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Virology 314:630-5. 2003
    ..These data suggest that therapeutic immunisation by PMID with papillomavirus early genes is effective and emphasizes the importance of antigen load in the generation of protective responses to papillomavirus proteins...
  30. pmc In vitro progression of human papillomavirus 16 episome-associated cervical neoplasia displays fundamental similarities to integrant-associated carcinogenesis
    Elizabeth Gray
    Medical Research Council Cancer Cell Unit, University of Cambridge, Cambridge, United Kingdom
    Cancer Res 70:4081-91. 2010
    ..Our findings suggest a unified working model for events important in cervical neoplastic progression regardless of HR-HPV physical state...
  31. ncbi request reprint Changes in cervical keratinocyte gene expression associated with integration of human papillomavirus 16
    William Alazawi
    Medical Research Council Cancer Cell Unit, MRC Hutchison Research Centre, Cambridge, United Kingdom
    Cancer Res 62:6959-65. 2002
    ..These genes include p48, a component of the primary regulator of the IFN response pathway, IFN-stimulated gene factor 3. The physical state of high-risk HPV may substantially influence the response to IFN in infected keratinocytes...
  32. ncbi request reprint Expression of the myelomonocytic antigens CD36 and L1 by keratinocytes in squamous intraepithelial lesions of the cervix
    N Coleman
    Department of Pathology, University of Cambridge, UK
    Hum Pathol 25:73-9. 1994
    ..CD36 may function as an immunoregulatory molecule on cervical keratinocytes in SILs, while L1 is more likely to be involved in the intracellular regulation of cell proliferation and maturation...
  33. ncbi request reprint Amplification of chromosome 5p correlates with increased expression of Skp2 in HPV-immortalized keratinocytes
    S E Dowen
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Oncogene 22:2531-40. 2003
    ..At medium to late passage, gain of 5p as an isochromosome was observed. Increased expression of Skp2 and a reduction in the expression of its target p27 correlated with increasing passage in this line...
  34. ncbi request reprint Altered expression of desmosomal components in high-grade squamous intraepithelial lesions of the cervix
    W O F Alazawi
    Medical Research Council Cancer Cell Unit, MRC Hutchison Research Centre, Hills Road, Cambridge, UK
    Virchows Arch 443:51-6. 2003
    ....
  35. ncbi request reprint Acquisition of high-level chromosomal instability is associated with integration of human papillomavirus type 16 in cervical keratinocytes
    Mark R Pett
    Medical Research Council Cancer Cell Unit, MRC Hutchison Research Centre, University of Cambridge, Hills Road, Cambridge CB2 2XZ, United Kingdom
    Cancer Res 64:1359-68. 2004
    ..HR-HPV integration is likely to be a critical event in cervical carcinogenesis, preceding the development of chromosomal abnormalities that drive malignant progression...
  36. ncbi request reprint In vivo and in vitro effects of v-fos and EJ-Ha-ras oncogene expression in murine epidermal keratinocytes
    M W Appleby
    Department of Pathology, Cambridge University, UK
    Oncogene 4:1323-30. 1989
    ..14 cm2 is required before a full thickness epithelium forms...
  37. doi request reprint A prospective study on the natural course of low-grade squamous intraepithelial lesions and the presence of HPV16 E2-, E6- and E7-specific T-cell responses
    Yin Ling Woo
    Department of Pathology, Cambridge University, Cambridge CB2 2QQ, UK
    Int J Cancer 126:133-41. 2010
    ..This was paralleled by immune failure as most of the patients with an HPV16+ LSIL failed to react to peptides of HPV16 E2, E6 or E7...
  38. doi request reprint Characterization of naturally occurring HPV16 integration sites isolated from cervical keratinocytes under noncompetitive conditions
    Keltie L Dall
    Medical Research Council Cancer Cell Unit, Hutchison Medical Research Council Research Center, University of Cambridge, Cambridge, United Kingdom
    Cancer Res 68:8249-59. 2008
    ..These latter observations do not support an important role for HPV16 integration in causing insertional mutagenesis...
  39. ncbi request reprint Protection of beagle dogs from mucosal challenge with canine oral papillomavirus by immunization with recombinant adenoviruses expressing codon-optimized early genes
    Kimberly B Johnston
    Vaccines and Biologics Research, Merck Research Laboratories, West Point, PA 19486, USA
    Virology 336:208-18. 2005
    ..These data indicate that rAd5 expressing papillomavirus E1 and E2 proteins can induce strong protective responses even in outbred populations under practical immunization conditions...
  40. pmc Papillomavirus-mediated neoplastic progression is associated with reciprocal changes in JAGGED1 and manic fringe expression linked to notch activation
    Karthikeyan Veeraraghavalu
    National Centre for Biological Sciences, TIFR, UAS GKVK Campus, Bangalore 560065, India
    J Virol 78:8687-700. 2004
    ..We suggest that activation of Notch in cervical neoplasia is Jagged1 dependent and that its susceptibility to the influence of Manic Fringe is of therapeutic value...
  41. ncbi request reprint Human papillomavirus (HPV) vaccines: prospects for eradicating cervical cancer
    Margaret A Stanley
    J Fam Plann Reprod Health Care 30:213-5. 2004
  42. ncbi request reprint Vaccinia-expressed human papillomavirus 16 and 18 e6 and e7 as a therapeutic vaccination for vulval and vaginal intraepithelial neoplasia
    Peter J Baldwin
    Department of Gynaecological Oncology, Addenbrooke s National Health Service Trust, Cambridge CB2 2QQ, United Kingdom
    Clin Cancer Res 9:5205-13. 2003
    ..We have tested the safety, immunogenicity, and efficacy of a recombinant vaccinia candidate vaccine (TA-HPV) in women with anogenital intraepithelial neoplasia...
  43. ncbi request reprint Human papillomavirus vaccination for the prevention of cervical neoplasia: is it appropriate to vaccinate women older than 26?
    S Rachel Skinner
    School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia
    Med J Aust 188:238-42. 2008
    ..It is likely that this vaccine will need to be purchased by women in the older age group (27-45 years)...
  44. pmc Human papillomavirus type 16 E6 amino acid 83 variants enhance E6-mediated MAPK signaling and differentially regulate tumorigenesis by notch signaling and oncogenic Ras
    Oishee Chakrabarti
    National Centre for Biological Sciences, TIFR, UAS GKVK Campus, Bangalore 560065, India
    J Virol 78:5934-45. 2004
    ....
  45. pmc Selection of cervical keratinocytes containing integrated HPV16 associates with episome loss and an endogenous antiviral response
    Mark R Pett
    Medical Research Council Cancer Cell Unit, Cambridge CB2 2XZ, United Kingdom
    Proc Natl Acad Sci U S A 103:3822-7. 2006
    ..We conclude that cervical carcinogenesis requires not only HRHPV integration, but also loss of inhibitory episomes...
  46. ncbi request reprint Interferon-beta treatment of cervical keratinocytes naturally infected with human papillomavirus 16 episomes promotes rapid reduction in episome numbers and emergence of latent integrants
    M Trent Herdman
    Medical Research Council Cancer Cell Unit, Cambridge CB2 2XZ, UK
    Carcinogenesis 27:2341-53. 2006
    ..Greater emphasis should be placed on episome loss in models of HPV-related carcinogenesis. We provide the strongest evidence to date that treating HPV-16 lesions by inducing an IFN response may cause clinical progression...