Research Topics
| M A StanleySummaryAffiliation: University of Cambridge Country: UK Publications
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Detail Information
Publications
Critical evaluation of HPV16 gene copy number quantification by SYBR green PCRIan Roberts
MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 0XZ, UK
BMC Biotechnol 8:57. 2008..We assessed a modified method, in which external calibration curves were generated from a single construct containing HPV16 E2, HPV16 E6 and the host gene hydroxymethylbilane synthase in a 1:1:1 ratio...
Prognostic factors and new therapeutic approaches to cervical cancerMargaret A Stanley
Department of Pathology, University of Cambridge, Tennis Court Road, UK
Virus Res 89:241-8. 2002....- Progress in prophylactic and therapeutic vaccines for human papillomavirus infectionMargaret A Stanley
Department of Pathology, Cambridge, UK
Expert Rev Vaccines 2:381-9. 2003..In Phase I and II trials these vaccines are immunogenic and safe but show limited efficacy... 
Human papillomavirus vaccines versus cervical cancer screeningM Stanley
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
Clin Oncol (R Coll Radiol) 20:388-94. 2008..However, if both vaccination and screening are combined then the virtual elimination of cervical cancer and the other HPV 16 and 18-associated cancers is possible...- HPV: from infection to cancerM A Stanley
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, U K
Biochem Soc Trans 35:1456-60. 2007.... - Prophylactic HPV vaccinesMargaret Stanley
Department of Pathology, University of Cambridge, Cambridge, UK
Drugs Today (Barc) 43:737-44. 2007..The health economic benefits will be substantial but the overwhelming benefit is the major improvement for human health and well-being. (.. - Prevention strategies against the human papillomavirus: the effectiveness of vaccinationM Stanley
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
Gynecol Oncol 107:S19-23. 2007..Predictive data also indicate that the implementation of HPV vaccination within a national screening program is likely to be cost-effective relative to current clinical practice... - HPV vaccines: where are we now?Margaret Stanley
Department of Pathology, University of Cambridge, Cambridge, UK
J Fam Plann Reprod Health Care 33:227-9. 2007 - Immune responses to human papillomavirusMargaret Stanley
University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
Vaccine 24:S16-22. 2006..Available data suggests that these vaccines are safe, produce high levels of antibodies, and are effective at preventing HPV infection... - HPV vaccinesMargaret Stanley
Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
Best Pract Res Clin Obstet Gynaecol 20:279-93. 2006..Therapeutic vaccines for low-grade intraepithelial disease are realistic but high-grade disease presents major hurdles for immunotherapies... - Prophylactic HPV vaccines: prospects for eliminating ano-genital cancerM Stanley
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
Br J Cancer 96:1320-3. 2007.... - Immunobiology of human papillomavirus infection and vaccination - implications for second generation vaccinesMargaret Stanley
Department of Pathology, University of Cambridge, Cambridge, United Kingdom
Vaccine 26:K62-7. 2008..This will be central in the development of adjuvants and vaccine formulations that induce the response to provide effective protection... - Imiquimod and the imidazoquinolones: mechanism of action and therapeutic potentialM A Stanley
University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
Clin Exp Dermatol 27:571-7. 2002..These compounds also have adjuvant properties that could significantly enhance conventional vaccine strategies... - Human papillomavirus vaccinesMargaret A Stanley
Department of Pathology, Cambridge, UK
Rev Med Virol 16:139-49. 2006..Second generation vaccines could include an early antigen for protection post-exposure and alternative delivery systems may be needed for the developing world... - A C-terminal helicase domain of the human papillomavirus E1 protein binds E2 and the DNA polymerase alpha-primase p68 subunitP J Masterson
Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
J Virol 72:7407-19. 1998..The modelling closely matches the deletion analysis in suggesting that this region of E1 is indeed a structural domain, and our results suggest that it is multifunctional and critical to several stages of HPV DNA replication... - Immune responses to human papilloma virusesM A Stanley
Department of Pathology, University of Cambridge, Cambridge, UK
Indian J Med Res 130:266-76. 2009..These vaccines are delivered intramuscularly and therefore circumvent the immune evasion strategies of the virus... - Human papillomavirus vaccinesMargaret A Stanley
Department of Pathology, University of Cambridge, UK
Curr Opin Mol Ther 4:15-22. 2002..Immunogenicity, but no efficacy, has been demonstrated in small clinical trials with some of these approaches... - Detection of viral DNA and E4 protein in basal keratinocytes of experimental canine oral papillomavirus lesionsP K Nicholls
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, United Kingdom
Virology 284:82-98. 2001..Detection of viral DNA in post-regression tissue indicated latent infection... - The immunology of animal papillomavirusesP K Nicholls
Department of Pathology, University of Cambridge, UK
Vet Immunol Immunopathol 73:101-27. 2000.... - Regression of canine oral papillomas is associated with infiltration of CD4+ and CD8+ lymphocytesP K Nicholls
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, United Kingdom
Virology 283:31-9. 2001..The lymphocyte infiltrate in the dog resembled that in human papillomavirus lesions, indicating that COPV is an appropriate model for human papillomavirus immunity... - Intra-epithelial vaccination with COPV L1 DNA by particle-mediated DNA delivery protects against mucosal challenge with infectious COPV in beagle dogsM A Stanley
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
Vaccine 19:2783-92. 2001..Both cell-mediated lymphoproliferative and humoral antibody responses to the DNA vaccine were observed. Our data indicate that PMDD of plasmid DNA can protect against mucosal challenge with papillomavirus... - Intraepithelial DNA immunisation with a plasmid encoding a codon optimised COPV E1 gene sequence, but not the wild-type gene sequence completely protects against mucosal challenge with infectious COPV in beaglesRichard A Moore
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
Virology 304:451-9. 2002.... - Cell-mediated immune responses to COPV early proteinsSuchitra Jain
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
Virology 356:23-34. 2006.... - An increase in DNA double-strand breaks, induced by Ku70 depletion, is associated with human papillomavirus 16 episome loss and de novo viral integration eventsD M Winder
Medical Research Council Cancer Cell Unit, MRC Hutchison Research Centre, Hills Road, Cambridge, CB2 0XZ, UK
J Pathol 213:27-34. 2007..We found no evidence to support the notion that major chromosomal instability precedes HPV16 integration, although such instability is an important consequence of the integration event... - Absence of canine oral papillomavirus DNA following prophylactic L1 particle-mediated immunotherapeutic delivery vaccinationR A Moore
Department of Pathology, Cambridge University, Cambridge CB2 1QP, UK
J Gen Virol 83:2299-301. 2002..These data support PMID of COPV L1 as a protective vaccine and suggest that PMID of L1 may induce virus clearance... - Human papillomavirus and cervical carcinogenesisM A Stanley
Department of Pathology, Tennis Court Road, Cambridge, CB2 1QP, UK
Best Pract Res Clin Obstet Gynaecol 15:663-76. 2001..These are rare events but viral persistence and chronic exposure to steroid hormones increase the probability of this deregulation... - HPV & head and neck cancer: a descriptive updatePeter K C Goon
Department of Pathology, University of Cambridge, Cambridge, UK
Head Neck Oncol 1:36. 2009..Finally, the improved prognosis and treatment responses to chemotherapy and radiotherapy by HPV+ tumours may suggest that HPV status detection is required to better plan and individualize patient treatment regimes... - Sensitive HPV detection in oropharyngeal cancersDavid M Winder
Dept of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
BMC Cancer 9:440. 2009..To help ascertain a useful PCR detection protocol for oropharyngeal cancers, we directly compared three commonly used primer sets in detection of HPV from different clinical samples... - Therapeutic immunisation with COPV early genes by epithelial DNA deliveryRichard A Moore
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
Virology 314:630-5. 2003..These data suggest that therapeutic immunisation by PMID with papillomavirus early genes is effective and emphasizes the importance of antigen load in the generation of protective responses to papillomavirus proteins... - In vitro progression of human papillomavirus 16 episome-associated cervical neoplasia displays fundamental similarities to integrant-associated carcinogenesisElizabeth Gray
Medical Research Council Cancer Cell Unit, University of Cambridge, Cambridge, United Kingdom
Cancer Res 70:4081-91. 2010..Our findings suggest a unified working model for events important in cervical neoplastic progression regardless of HR-HPV physical state... - Changes in cervical keratinocyte gene expression associated with integration of human papillomavirus 16William Alazawi
Medical Research Council Cancer Cell Unit, MRC/Hutchison Research Centre, Cambridge, United Kingdom
Cancer Res 62:6959-65. 2002..These genes include p48, a component of the primary regulator of the IFN response pathway, IFN-stimulated gene factor 3. The physical state of high-risk HPV may substantially influence the response to IFN in infected keratinocytes... - Expression of the myelomonocytic antigens CD36 and L1 by keratinocytes in squamous intraepithelial lesions of the cervixN Coleman
Department of Pathology, University of Cambridge, UK
Hum Pathol 25:73-9. 1994..CD36 may function as an immunoregulatory molecule on cervical keratinocytes in SILs, while L1 is more likely to be involved in the intracellular regulation of cell proliferation and maturation... - Amplification of chromosome 5p correlates with increased expression of Skp2 in HPV-immortalized keratinocytesS E Dowen
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
Oncogene 22:2531-40. 2003..At medium to late passage, gain of 5p as an isochromosome was observed. Increased expression of Skp2 and a reduction in the expression of its target p27 correlated with increasing passage in this line... - Altered expression of desmosomal components in high-grade squamous intraepithelial lesions of the cervixW O F Alazawi
Medical Research Council Cancer Cell Unit, MRC/Hutchison Research Centre, Hills Road, Cambridge, UK
Virchows Arch 443:51-6. 2003.... - Acquisition of high-level chromosomal instability is associated with integration of human papillomavirus type 16 in cervical keratinocytesMark R Pett
Medical Research Council Cancer Cell Unit, MRC/Hutchison Research Centre, University of Cambridge, Hills Road, Cambridge CB2 2XZ, United Kingdom
Cancer Res 64:1359-68. 2004..HR-HPV integration is likely to be a critical event in cervical carcinogenesis, preceding the development of chromosomal abnormalities that drive malignant progression... - In vivo and in vitro effects of v-fos and EJ-Ha-ras oncogene expression in murine epidermal keratinocytesM W Appleby
Department of Pathology, Cambridge University, UK
Oncogene 4:1323-30. 1989..14 cm2 is required before a full thickness epithelium forms... - A prospective study on the natural course of low-grade squamous intraepithelial lesions and the presence of HPV16 E2-, E6- and E7-specific T-cell responsesYin Ling Woo
Department of Pathology, Cambridge University, Cambridge CB2 2QQ, UK
Int J Cancer 126:133-41. 2010..This was paralleled by immune failure as most of the patients with an HPV16+ LSIL failed to react to peptides of HPV16 E2, E6 or E7... - Characterization of naturally occurring HPV16 integration sites isolated from cervical keratinocytes under noncompetitive conditionsKeltie L Dall
Medical Research Council Cancer Cell Unit, Hutchison Medical Research Council Research Center, University of Cambridge, Cambridge, United Kingdom
Cancer Res 68:8249-59. 2008..These latter observations do not support an important role for HPV16 integration in causing insertional mutagenesis... - Protection of beagle dogs from mucosal challenge with canine oral papillomavirus by immunization with recombinant adenoviruses expressing codon-optimized early genesKimberly B Johnston
Vaccines and Biologics Research, Merck Research Laboratories, West Point, PA 19486, USA
Virology 336:208-18. 2005..These data indicate that rAd5 expressing papillomavirus E1 and E2 proteins can induce strong protective responses even in outbred populations under practical immunization conditions... - Papillomavirus-mediated neoplastic progression is associated with reciprocal changes in JAGGED1 and manic fringe expression linked to notch activationKarthikeyan Veeraraghavalu
National Centre for Biological Sciences, TIFR, UAS GKVK Campus, Bangalore 560065, India
J Virol 78:8687-700. 2004..We suggest that activation of Notch in cervical neoplasia is Jagged1 dependent and that its susceptibility to the influence of Manic Fringe is of therapeutic value... - Human papillomavirus (HPV) vaccines: prospects for eradicating cervical cancerMargaret A Stanley
J Fam Plann Reprod Health Care 30:213-5. 2004 - Vaccinia-expressed human papillomavirus 16 and 18 e6 and e7 as a therapeutic vaccination for vulval and vaginal intraepithelial neoplasiaPeter J Baldwin
Department of Gynaecological Oncology, Addenbrooke's National Health Service Trust, Cambridge CB2 2QQ, United Kingdom
Clin Cancer Res 9:5205-13. 2003.... - Human papillomavirus vaccination for the prevention of cervical neoplasia: is it appropriate to vaccinate women older than 26?S Rachel Skinner
School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia
Med J Aust 188:238-42. 2008..It is likely that this vaccine will need to be purchased by women in the older age group (27-45 years)... - Human papillomavirus type 16 E6 amino acid 83 variants enhance E6-mediated MAPK signaling and differentially regulate tumorigenesis by notch signaling and oncogenic RasOishee Chakrabarti
National Centre for Biological Sciences, TIFR, UAS GKVK Campus, Bangalore 560065, India
J Virol 78:5934-45. 2004.... - Selection of cervical keratinocytes containing integrated HPV16 associates with episome loss and an endogenous antiviral responseMark R Pett
Medical Research Council Cancer Cell Unit, Cambridge CB2 2XZ, United Kingdom
Proc Natl Acad Sci U S A 103:3822-7. 2006..We conclude that cervical carcinogenesis requires not only HRHPV integration, but also loss of inhibitory episomes... - Interferon-beta treatment of cervical keratinocytes naturally infected with human papillomavirus 16 episomes promotes rapid reduction in episome numbers and emergence of latent integrantsM Trent Herdman
Medical Research Council Cancer Cell Unit, Cambridge CB2 2XZ, UK
Carcinogenesis 27:2341-53. 2006..Greater emphasis should be placed on episome loss in models of HPV-related carcinogenesis. We provide the strongest evidence to date that treating HPV-16 lesions by inducing an IFN response may cause clinical progression...