Affiliation: University of St Andrews
- Using oocyte nuclei for studies on chromatin structure and gene expressionJohn Sommerville
School of Biology, Bute Medical Buildings, University of St Andrews, Westburn Lane, St Andrews, Fife, Scotland, UK
Methods 51:157-64. 2010..As an aid to the targeting of the GV within the nucleus, descriptions are given of the use of oocytes from albino animals...
- Xp54 and related (DDX6-like) RNA helicases: roles in messenger RNP assembly, translation regulation and RNA degradationAndrew Weston
School of Biology, University of St Andrews, St Andrews, Fife KY16 9TS, Scotland, UK
Nucleic Acids Res 34:3082-94. 2006..The versatility of Xp54 and related helicases in modulating the metabolism of mRNAs at all stages of their lifetimes marks them out as key regulators of post-transcriptional gene expression...
- Activities of cold-shock domain proteins in translation controlJ Sommerville
School of Biomedical Sciences, University of St Andrews, Scotland, United Kingdom
Bioessays 21:319-25. 1999..It is argued here that the loading of CSD proteins on mRNA and the stability of the protein/mRNA complex are regulated by RNA helicase activity and protein phosphorylation...
- Signal recognition particle assembly in relation to the function of amplified nucleoli of Xenopus oocytesJohn Sommerville
Division of Cell and Molecular Biology, School of Biology, University of St Andrews, KY16 9TS, UK
J Cell Sci 118:1299-307. 2005....
- 4SR, a novel zinc-finger protein with SR-repeats, is expressed during early development of XenopusM Ladomery
Division of Cell and Molecular Biology, University of St Andrews, School of Biological and Medical Sciences, Westburn Lane, Bute Medical Buildings C17, KY16 9TS, Fife, UK
Gene 256:293-302. 2000..We suggest the descriptor (DW)C(4), in which DW refers to the invariant aspartic acid (D)/tryptophan (W) dipeptide that precedes the first cysteine residue, for this distinctive zinc-finger structure...
- Maternal histone deacetylase is accumulated in the nuclei of Xenopus oocytes as protein complexes with potential enzyme activityJ Ryan
School of Biomedical Sciences, Bute Medical Buildings, University of St Andrews, St Andrews, Fife KY16 9TS, UK
J Cell Sci 112:2441-52. 1999..However, recombinant HDACm, injected into the cytoplasm of oocytes, not only is translocated to the nucleus, but also is free to interact with the endogenous chromatin...
- Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytesM Ladomery
School of Biological and Medical Sciences, Bute Buildings, University of St Andrews, St Andrews, Fife KY16 9TS, UK
Nucleic Acids Res 25:965-73. 1997..On isolating Xp54 from mRNP particles, it is shown to possess an ATP-dependent RNA helicase activity. Possible functions of Xp54 are discussed in relation to the assembly and utilization of mRNP particles...
- RNA helicase p54 (DDX6) is a shuttling protein involved in nuclear assembly of stored mRNP particlesDavid A Smillie
Division of Cell and Molecular Biology, School of Biology, University of St Andrews, St Andrews, Fife KY16 9TS, Scotland
J Cell Sci 115:395-407. 2002....
- Xenopus HDm, a maternally expressed histone deacetylase, belongs to an ancient family of acetyl-metabolizing enzymesM Ladomery
Division of Cell and Molecular Biology, School of Biological and Medical Sciences, University of St Andrews, Fife, Scotland, UK
Gene 198:275-80. 1997....
- Nuclear import and activity of histone deacetylase in Xenopus oocytes is regulated by phosphorylationDavid A Smillie
Division of Cell and Molecular Biology, School of Biology, University of St Andrews, St Andrews, Fife KY16 9TS, UK
J Cell Sci 117:1857-66. 2004..Thus, both translocation and activity of HDACm appear to be regulated by specific phosphorylation events. These results have implications for techniques involving the transfer of somatic nuclei into enucleated oocytes...
- Expression in Xenopus oocytes shows that WT1 binds transcripts in vivo, with a central role for zinc finger oneMichael Ladomery
MRC Human Genetics Unit, Western General Hospital, Crewe Rd, Edinburgh EH4 2XU, UK
J Cell Sci 116:1539-49. 2003..We suggest that in evolution, WT1 has acquired the ability to interact with transcripts and splice factors because of the modification of zinc finger one and the +KTS alternative splice...
- Dynamic regulation of histone modifications in Xenopus oocytes through histone exchangeM David Stewart
Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Mol Cell Biol 26:6890-901. 2006..Taken together, our data suggest that chromatin-associated and non-chromatin-associated histones are continually exchanged in the Xenopus oocyte, creating a highly dynamic chromatin environment...