Research Topics
Genomes and Genes | Godfrey SmithSummaryAffiliation: University of Glasgow Country: UK Publications
| Collaborators
|
Detail Information
Publications
Selective down-regulation of sub-endocardial ryanodine receptor expression in a rabbit model of left ventricular dysfunctionSusan Currie
Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
J Mol Cell Cardiol 39:309-17. 2005..There is a corresponding decrease in fractional SR Ca2+ release in cells isolated from the sub-endocardium of hearts from the LVD group, relating these selective biochemical alterations to changes in function...- Mapping of epicardial activation in a rabbit model of chronic myocardial infarction:Nicola L Walker
Division of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK
J Cardiovasc Electrophysiol 18:862-8. 2007..This study examines the consequences of a large transmural apical infarct on the epicardial electrical activity in isolated rabbit hearts... 
The link between repolarisation alternans and ventricular arrhythmia: does the cellular phenomenon extend to the clinical problem?Rachel C Myles
BHF Glasgow Cardiovascular Research Centre, University of Glasgow, University Place, Glasgow, G12 8TA, UK
J Mol Cell Cardiol 45:1-10. 2008..The areas where experimental evidence is weak are highlighted, and areas for future research are outlined...
The effects of over-expression of the FK506-binding protein FKBP12.6 on K(+) currents in adult rabbit ventricular myocytesS Kettlewell
Institute of Biomedical and Life Sciences, University of Glasgow, University Avenue, Glasgow, G12 8QQ, Scotland, UK
Pflugers Arch 458:653-60. 2009..These data suggest that FKBP12.6 can modulate APD via changes in I (K1) independently of calcineurin binding, suggesting that FKBP12.6 may affect APD by direct interaction with I (K1)...- Measurement of SR Ca2+ content in the presence of caffeine in permeabilised rat cardiac trabeculaeG L Smith
Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow, G12 8QQ, UK
Pflugers Arch 437:139-48. 1998..6 mmol/l SR volume)... - Cytoplasmic versus intra-SR: the battle of the Ca2+ diffusion coefficients in cardiac muscleGodfrey Smith
Biomedical and Life Sciences, Glasgow University, Glasgow, United Kingdom
Biophys J 95:1005-6. 2008 - Na(+)/Ca(2+) exchanger expression and function in a rabbit model of myocardial infarctionGodfrey L Smith
Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
J Cardiovasc Electrophysiol 17:S57-S63. 2006..However, in a subset of studies, reduced activity of NCX has been reported. Left ventricular dysfunction (LVD) was induced in the rabbit eight weeks after an apical myocardial infarction... - Matters of the heart: the physiology of cardiac function and failureGodfrey Smith
Institute of Biomedical and Life Sciences IBLS, University of Glasgow, Glasgow G12 8QQ, UK
Exp Physiol 92:973-86. 2007..ii) The use of regular exercise training to improve cardiac performance in heart failure. This appears to act by normalizing a number of aspects of myocardial physiology... - Differential sensitivity of Ca²+ wave and Ca²+ spark events to ruthenium red in isolated permeabilised rabbit cardiomyocytesN Macquaide
Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow G12 8QQ, UK
J Physiol 588:4731-42. 2010..Therefore inhibition of Ca²(+) waves without affecting Ca²(+) sparks may be explained by block of small, non-spark producing clusters of RyRs that are important to the process of Ca²(+) wave propagation... - P(i) inhibits the SR Ca(2+) pump and stimulates pump-mediated Ca(2+) leak in rabbit cardiac myocytesG L Smith
Institute of Biomedical and Life Sciences, Glasgow University, Glasgow G12 8QQ, Scotland
Am J Physiol Heart Circ Physiol 279:H577-85. 2000..In conclusion, P(i) decreases the Ca(2+) uptake capacity of cardiac SR via a decrease in pump rate and an increase in Ca(2+) pump-dependent Ca(2+) leak... - A comparison of the effects of ATP and tetracaine on spontaneous Ca(2+) release from rat permeabilised cardiac myocytesG L Smith
Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
J Physiol 534:37-47. 2001..But recovery after incubation with 300 microM tetracaine resulted in SR Ca(2+) release with no coherent wave pattern. The reason for this discrepancy is discussed... - Effect of activation sequence on transmural patterns of repolarization and action potential duration in rabbit ventricular myocardiumRachel C Myles
British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, United Kingdom
Am J Physiol Heart Circ Physiol 299:H1812-22. 2010.... - Metabolic monitoring of the electrically stimulated single heart cell within a microfluidic platformWei Cheng
Bioelectronics Research Centre, Department of Electronics and Electrical Engineering, University of Glasgow, Glasgow, UK
Lab Chip 6:1424-31. 2006..Finally, the relative amounts of intra- and extra-cellular lactate produced during field stimulation were determined, using cell electroporation where necessary... - Extracellular recordings of field potentials from single cardiomyocytesNorbert Klauke
Department of Electronics, University of Glasgow, Glasgow G12 8LT, United Kingdom
Biophys J 91:2543-51. 2006..This data demonstrate the feasibility of the use of a microfluidic bath design to limit the extracellular resistance between two ends of an isolated cardiomyocyte... - Effects of calsequestrin over-expression on excitation-contraction coupling in isolated rabbit cardiomyocytesStewart L W Miller
Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
Cardiovasc Res 67:667-77. 2005..CONCLUSIONS: CSQ over-expression increased SR Ca2+ content but reduced the gain of E-C coupling in rabbit cardiomyocytes... - Microfluidic cell arrays for metabolic monitoring of stimulated cardiomyocytesWei Cheng
Department of Electronics, University of Glasgow, UK
Electrophoresis 31:1405-13. 2010..This array system has the potential of simultaneous electrochemical and optical monitoring of extracellular and intracellular metabolites from single beating heart cells at a controlled metabolic state... - Dysregulation of cadherins in the intercalated disc of the spontaneously hypertensive stroke-prone ratMargaret Anne Craig
BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK
J Mol Cell Cardiol 48:1121-8. 2010..Therefore, remodelling of the intercalated disc in the hearts of SHRSP animals may contribute to the altered function observed in these animals... - Confocal and multiphoton imaging of intracellular Ca(2+)Godfrey Smith
School of Life Sciences, University of Glasgow, United Kingdom
Methods Cell Biol 99:225-61. 2010..Finally, the combined emission spectra of Ca(2+) and voltage sensitive dyes are compared to allow the choice of the optimum combination to allow simultaneous intracellular Ca(2+) and membrane voltage measurement... - Assessment of sarcoplasmic reticulum Ca(2+) flux pathways in cardiomyocytes from rabbits with infarct-induced left-ventricular dysfunctionPaul Neary
Department of Medical Cardiology, Glasgow Royal Infirmary, Glasgow G31 2ER, UK
Pflugers Arch 444:360-71. 2002..Reduced background Ca(2+) leak from the SR may compensate partly for the reduced Ca(2+) uptake capacity of the SR in the LVD group... - Evidence of intercellular coupling between co-cultured adult rabbit ventricular myocytes and myofibroblastsLisa Chilton
Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow G12 8QQ, UK
J Physiol 583:225-36. 2007..This work indicates the nature of the gap junction-mediated bi-directional interactions that occur between ventricular myocyte and myofibroblast... - Activation or inactivation of cardiac Akt/mTOR signaling diverges physiological from pathological hypertrophyOle Johan Kemi
Institute of Biomedical and Life Sciences, University of Glasgow, UK
J Cell Physiol 214:316-21. 2008.... - Temperature preconditioning: a cold-hearted answer to ischaemic reperfusion injury?Iffath A Ghouri
Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
J Physiol 585:649-50. 2007 - Exercise-induced changes in calcium handling in left ventricular cardiomyocytesOle J Kemi
Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
Front Biosci 13:356-68. 2008..In particular, exercise training is able to restore depressed contractility and calcium cycling associated with heart failure, to a value comparable to healthy individuals... - Myocardial sarcoplasmic reticulum Ca2+ ATPase function is increased by aerobic interval trainingOle Johan Kemi
Institute of Biomedical and Life Sciences, University of Glasgow, UK
Eur J Cardiovasc Prev Rehabil 15:145-8. 2008..Exercise training improves myocardial Ca-handling, but SERCA-2a function is uncertain. We assessed SERCA-2a activity after exercise training... - Depressed inotropic response to increased preload in rabbit hearts with left-ventricular dysfunction induced by chronic myocardial infarctionG Andre Ng
Deparment of Medical Cardiology, University of Glasgow, Glasgow, G12 8 QQ, UK
Pflugers Arch 444:513-22. 2002..These changes appear not to be accompanied by altered passive mechanical properties... - Microfluidic partitioning of the extracellular space around single cardiac myocytesNorbert Klauke
Department of Electronics, University of Glasgow, Glasgow G12 8LT, Scotland
Anal Chem 79:1205-12. 2007.... - Abnormal contractile activity and calcium cycling in cardiac myocytes isolated from DMPK knockout miceGurman S Pall
Division of Molecular Genetics, Faculty of Biomedical and Life Sciences, Anderson College, University of Glasgow, Scotland, United Kingdom
Physiol Genomics 13:139-46. 2003..These observations suggest dmpk has a modulatory role in the control of intracellular Ca(2+) concentration in mouse ventricular cardiomyocytes, loss of which may contribute to cardiac dysfunction in DM1... - Stimulation of single isolated adult ventricular myocytes within a low volume using a planar microelectrode arrayNorbert Klauke
Department of Electronics, University of Glasgow, Glasgow, United Kingdom
Biophys J 85:1766-74. 2003..Whereas this arrangement could be used to create a screening assay for drugs, individual microchannels (100 pL) can also be used to study the effects of limited extracellular volume on the contractility of single cardiac myocytes... - Calcium/calmodulin-dependent protein kinase IIdelta associates with the ryanodine receptor complex and regulates channel function in rabbit heartSusan Currie
Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow G12 8QQ, UK
Biochem J 377:357-66. 2004..These results suggest the existence of an endogenous CaMKIIdelta that associates directly with RyR2 and specifically modulates RyR2 activity... - Aerobic interval training enhances cardiomyocyte contractility and Ca2+ cycling by phosphorylation of CaMKII and Thr-17 of phospholambanOle J Kemi
Department of Circulation and Medical Imaging, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
J Mol Cell Cardiol 43:354-61. 2007..These observations indicate that calcium/calmodulin-dependent kinase IIdelta contributes significantly to the functional adaptation of the cardiomyocyte to regular exercise training... - Characteristics of the in vitro hypoxic pulmonary vasoconstrictor response in isolated equine and bovine pulmonary arterial ringsKaren E MacEachern
University of Glasgow Veterinary School, Bearsden, Glasgow, Scotland G61 1QH, UK
Vet Anaesth Analg 31:239-49. 2004..This study compared the HPV response in isolated pulmonary artery vessels from equivalent regions of equine and bovine lung... - Stimulation of isolated ventricular myocytes within an open architecture microarrayNorbert Klauke
Department of Elecronics and Electrical Engineering, Rankine Building, University of Glasgow, Glasgow G12 8LT, UK
IEEE Trans Biomed Eng 52:531-8. 2005.... - Sorcin modulates cardiac L-type Ca2+ current by functional interaction with the alpha1C subunit in rabbitsMark R Fowler
Faculty of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow G12 8QQ, UK
Exp Physiol 93:1233-8. 2008..These data suggest that sorcin interacts with LTCC via its C-terminal domain, which alters current magnitude and tau(fast). These effects appear to be influenced by the prevailing experimental conditions... - Onset of experimental severe cardiac fibrosis is mediated by overexpression of Angiotensin-converting enzyme 2Rachel Masson
BHF GCRC, University of Glasgow, 126 University Place, Glasgow, G12 8TA United Kingdom
Hypertension 53:694-700. 2009..This study demonstrates that sustained overexpression of ACE2 in the heart in vivo leads to the onset of severe fibrosis... - Complex modulation of L-type Ca(2+) current inactivation by sorcin in isolated rabbit cardiomyocytesMark R Fowler
University of Glasgow, UK
Pflugers Arch 457:1049-60. 2009..Under normal conditions, sorcin slows I (Ca,L) inactivation because the effects of Ca(2+)-dependant inactivation out-weigh the effects on voltage-dependant inactivation... - Microfluidic systems to examine intercellular coupling of pairs of cardiac myocytesNorbert Klauke
Department of Electronics, University of Glasgow, Glasgow, UK G12 8LT
Lab Chip 7:731-9. 2007..Using this platform, it is also possible to make simultaneous optical recordings (including fluorescence and cell contraction) to explore the effect of drugs on one or both cells, within the doublet... - cGMP signals modulate cAMP levels in a compartment-specific manner to regulate catecholamine-dependent signaling in cardiac myocytesAlessandra Stangherlin
Institute of Neuroscience and Psychology, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom
Circ Res 108:929-39. 2011..cAMP and cGMP are intracellular second messengers involved in heart pathophysiology. cGMP can potentially affect cAMP signals via cGMP-regulated phosphodiesterases (PDEs)... - Heterogeneity of ventricular fibrillation dominant frequency during global ischemia in isolated rabbit heartsJane Caldwell
Division of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK
J Cardiovasc Electrophysiol 18:854-61. 2007..Ventricular fibrillation (VF) studies show that ECG-dominant frequency (DF) decreases as ischemia develops. This study investigates the contribution of the principle ischemic metabolic components to this decline... - Ultra-low-volume, real-time measurements of lactate from the single heart cell using microsystems technologyXinxia Cai
Bioelectronics Research Centre, Department of Electronics and Electrical Engineering, University of Glasgow, UK
Anal Chem 74:908-14. 2002..We also show the use of respiratory uncouplers to simulate ischemia in the single myocyte and show that, as expected, the rate of lactate production from the hypoxic heart cell is greater than that within the normoxic healthy myocyte... - The C terminus (amino acids 75-94) and the linker region (amino acids 42-54) of the Ca2+-binding protein S100A1 differentially enhance sarcoplasmic Ca2+ release in murine skinned skeletal muscle fibersPatrick Most
Abteilung Innere Medizin III Kardiologie, Universitat Heidelberg, 69115 Heidelberg, Germany
J Biol Chem 278:26356-64. 2003..Thus, our data suggest that S100A1 may serve as an endogenous enhancer of SR Ca2+ release and might therefore be of physiological relevance in the process of excitation-contraction coupling in skeletal muscle... - Aerobic exercise reduces cardiomyocyte hypertrophy and increases contractility, Ca2+ sensitivity and SERCA-2 in rat after myocardial infarctionUlrik Wisløff
Department of Physiology and Biomedical Engineering, Norwegian University of Science and Technology, Olav Kyrres gt 3, N 7489 Trondheim, Norway
Cardiovasc Res 54:162-74. 2002.... - Effects of adenovirus-mediated sorcin overexpression on excitation-contraction coupling in isolated rabbit cardiomyocytesTim Seidler
Department of Cardiology and Pneumology, Georg-August-University Goettingen, Goettingen, Germany
Circ Res 93:132-9. 2003..The effect of sorcin overexpression on Ca2+ sparks indicates an effect on the ryanodine receptor that may also influence excitation-contraction coupling... - Excessive sarcoplasmic/endoplasmic reticulum Ca2+-ATPase expression causes increased sarcoplasmic reticulum Ca2+ uptake but decreases myocyte shorteningNils Teucher
Department of Cardiology and Pneumology, University of Goettingen, Goettingen, Germany
Circulation 110:3553-9. 2004..We investigated the effects of different levels of SERCA1a expression on contractility and Ca2+ cycling. We tested whether increased SERCA1a expression levels enhance myocyte contractility in a gene-dose-dependent manner... - S100A1 decreases calcium spark frequency and alters their spatial characteristics in permeabilized adult ventricular cardiomyocytesMirko Völkers
Department of Internal Medicine III, Laboratory for Cardiac Stem Cell and Gene Therapy, Division of Cardiology, INF 350, University of Heidelberg, 69120 Heidelberg, Germany
Cell Calcium 41:135-43. 2007..6) remained unaltered by S100A1. Hence, we propose S100A1 as a novel inhibitory modulator of RyR2 function at diastolic Ca2+-concentrations in rabbit ventricular cardiomyocytes... - Carbon monoxide levels experienced by heavy smokers impair aerobic capacity and cardiac contractility and induce pathological hypertrophyAnja Bye
Department of Circulation and Medical Imaging, Faculty of Medicine, The Norwegian University of Science and Technology NTNU, Trondheim, Norway
Inhal Toxicol 20:635-46. 2008..Long-term CO exposure reduces aerobe capacity and contractile function and leads to pathological hypertrophy. Impaired Ca2 + handling and increased growth factor signaling seem to be responsible for these pathological changes... - The W105G and W99G sorcin mutants demonstrate the role of the D helix in the Ca(2+)-dependent interaction with annexin VII and the cardiac ryanodine receptorGianni Colotti
Consiglio Nazionale delle Ricerche, Institute of Molecular Biology and Pathology, Department of Biochemical Sciences A. Rossi Fanelli, University of Rome La Sapienza, 00185 Rome, Italy
Biochemistry 45:12519-29. 2006..The same activation process may apply to other PEF proteins in view of the D helix conservation... - Nitric oxide synthase type-1 modulates cardiomyocyte contractility and calcium handling: association with low intrinsic aerobic capacityMorten A Høydal
Department of Circulation and Medical Imaging, Faculty of Medicine, Norwegian University of Science and Technology, St Olavs Hospital, Trondheim, Norway
Eur J Cardiovasc Prev Rehabil 14:319-25. 2007..Our working hypothesis was that NOS-1 modulates cardiomyocyte contractility more markedly in rat lines with low versus high congenital aerobic fitness... - Superior cardiovascular effect of aerobic interval training versus moderate continuous training in heart failure patients: a randomized studyUlrik Wisløff
Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Olav Kyrres gt 9, 7489 Trondheim, Norway
Circulation 115:3086-94. 2007..Our objective was to compare training programs with moderate versus high exercise intensity with regard to variables associated with cardiovascular function and prognosis in patients with postinfarction heart failure... - Negative inotropy of the gastric proton pump inhibitor pantoprazole in myocardium from humans and rabbits: evaluation of mechanismsWolfgang Schillinger
Herzzentrum, Kardiologie und Pneumologie, Georg August Universitaet Goettingen, Robert Koch Strasse 40, 37099 Goettingen, Germany
Circulation 116:57-66. 2007..Proton pump inhibitors are used extensively for acid-related gastrointestinal diseases. Their effect on cardiac contractility has not been assessed directly... - Overexpression of FK-506 binding protein 12.0 modulates excitation contraction coupling in adult rabbit ventricular cardiomyocytesTim Seidler
Department of Cardiology and Pneumology, Georg August University Goettingen, Germany
Circ Res 101:1020-9. 2007..6 isomer. In conclusion, FKBP12.0-RyR2 interaction can regulate the gain of excitation-contraction coupling... - How does thrombolysis increase defibrillation success?Jane Cochrane Caldwell
Med Hypotheses 68:1183-4. 2007 - Electrical coupling of fibroblasts and myocytes: relevance for cardiac propagationPeter Kohl
University Laboratory of Physiology, OX1 3PT Oxford, UK
J Electrocardiol 38:45-50. 2005..This article reviews the currently available experimental and theoretical evidence on the previous scenarios, and highlights areas for further research...