Austin Smith

Summary

Affiliation: University of Edinburgh
Country: UK

Publications

  1. pmc Niche-independent symmetrical self-renewal of a mammalian tissue stem cell
    Luciano Conti
    Institute for Stem Cell Research, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Biol 3:e283. 2005
  2. pmc Notch promotes neural lineage entry by pluripotent embryonic stem cells
    Sally Lowell
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Biol 4:e121. 2006
  3. ncbi request reprint The battlefield of pluripotency
    Austin Smith
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, United Kingdom
    Cell 123:757-60. 2005
  4. ncbi request reprint Embryo-derived stem cells: of mice and men
    A G Smith
    Centre for Genome Research, University of Edinburgh, The King s Buildings, West Mains Road, Edinburgh, Scotland, EH9 3JQ, United Kingdom
    Annu Rev Cell Dev Biol 17:435-62. 2001
  5. ncbi request reprint BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, EH9 3JQ, Edinburgh, Scotland
    Cell 115:281-92. 2003
  6. ncbi request reprint Nanog safeguards pluripotency and mediates germline development
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 450:1230-4. 2007
  7. ncbi request reprint Nanog promotes transfer of pluripotency after cell fusion
    Jose Silva
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, University of Edinburgh, Edinburgh, EH9 3JQ, UK
    Nature 441:997-1001. 2006
  8. pmc Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice
    Jerome Aubert
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, EH9 3JQ Edinburgh, Scotland
    Proc Natl Acad Sci U S A 100:11836-41. 2003
  9. ncbi request reprint Self-renewal of teratocarcinoma and embryonic stem cells
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Rd, EH9 3JQ, Scotland, UK
    Oncogene 23:7150-60. 2004
  10. ncbi request reprint Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003

Collaborators

Detail Information

Publications30

  1. pmc Niche-independent symmetrical self-renewal of a mammalian tissue stem cell
    Luciano Conti
    Institute for Stem Cell Research, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Biol 3:e283. 2005
    ..These homogenous cultures will enable delineation of molecular mechanisms that define a tissue-specific stem cell and allow direct comparison with pluripotent ES cells...
  2. pmc Notch promotes neural lineage entry by pluripotent embryonic stem cells
    Sally Lowell
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Biol 4:e121. 2006
    ..Limiting activation of endogenous Notch results in heterogeneous lineage commitment. Manipulation of Notch signalling is therefore likely to be a key factor in taking command of ES cell lineage choice...
  3. ncbi request reprint The battlefield of pluripotency
    Austin Smith
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, United Kingdom
    Cell 123:757-60. 2005
    ..The vulnerability of lineage potential to transcriptional perturbation may reflect an essential feature of pluripotency...
  4. ncbi request reprint Embryo-derived stem cells: of mice and men
    A G Smith
    Centre for Genome Research, University of Edinburgh, The King s Buildings, West Mains Road, Edinburgh, Scotland, EH9 3JQ, United Kingdom
    Annu Rev Cell Dev Biol 17:435-62. 2001
    ....
  5. ncbi request reprint BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, EH9 3JQ, Edinburgh, Scotland
    Cell 115:281-92. 2003
    ..Upon LIF withdrawal, Id-expressing ES cells differentiate but do not give rise to neural lineages. We conclude that blockade of lineage-specific transcription factors by Id proteins enables the self-renewal response to LIF/STAT3...
  6. ncbi request reprint Nanog safeguards pluripotency and mediates germline development
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 450:1230-4. 2007
    ..We surmise that Nanog stabilizes embryonic stem cells in culture by resisting or reversing alternative gene expression states...
  7. ncbi request reprint Nanog promotes transfer of pluripotency after cell fusion
    Jose Silva
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, University of Edinburgh, Edinburgh, EH9 3JQ, UK
    Nature 441:997-1001. 2006
    ..We conclude that Nanog can orchestrate ES cell machinery to instate pluripotency with an efficiency of up to 100% depending on the differentiation status of the somatic cell...
  8. pmc Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice
    Jerome Aubert
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, EH9 3JQ Edinburgh, Scotland
    Proc Natl Acad Sci U S A 100:11836-41. 2003
    ..Whole mount in situ hybridization against two genes of previously unknown embryonic expression, Lrrn1 and Musashi2, confirmed the selectivity of this screen for early neuroectodermal markers...
  9. ncbi request reprint Self-renewal of teratocarcinoma and embryonic stem cells
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Rd, EH9 3JQ, Scotland, UK
    Oncogene 23:7150-60. 2004
    ..Nanog function also requires Oct4. Here, we review recent progress in ES cell self-renewal, relate this to the biology of teratocarcinomas and offer testable hypotheses to expose the mechanics of ES cell self-renewal...
  10. ncbi request reprint Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003
    ..These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity...
  11. ncbi request reprint Adherent neural stem (NS) cells from fetal and adult forebrain
    Steven M Pollard
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, Edinburgh EH9 3JQ, UK
    Cereb Cortex 16:i112-20. 2006
    ..We also characterize the growth factor requirements for NS cell derivation and self-renewal. We discuss our current understanding of the relationship of NS cell lines to physiological progenitor cells of fetal and adult CNS...
  12. ncbi request reprint Exploitation of adherent neural stem cells in basic and applied neurobiology
    Steven Pollard
    University of Edinburgh, Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
    Regen Med 1:111-8. 2006
    ..Longer term, NS cells offer an opportunity to rigorously test the efficacy of cell-based therapies and develop strategies for tissue engineering...
  13. doi request reprint Capture of authentic embryonic stem cells from rat blastocysts
    Mia Buehr
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, Edinburgh EH93JQ, UK
    Cell 135:1287-98. 2008
    ..Rat ES cells will also provide a refined test-bed for functional evaluation of pluripotent stem cell-derived tissue repair and regeneration...
  14. ncbi request reprint FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment
    Tilo Kunath
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
    Development 134:2895-902. 2007
    ....
  15. ncbi request reprint Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, United Kingdom
    Nat Biotechnol 21:183-6. 2003
    ..This system provides a platform for defining the molecular machinery of neural commitment and optimizing the efficiency of neuronal and glial cell production from pluripotent mammalian stem cells...
  16. pmc Genesis of embryonic stem cells
    Mia Buehr
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Philos Trans R Soc Lond B Biol Sci 358:1397-402; discussion 1402. 2003
    ..Procedures that extend this time-window and/or directly regulate the critical genes should increase the efficiency of ES cell derivation...
  17. ncbi request reprint Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
    H Niwa
    Centre for Genome Research, The University of Edinburgh, King s Buildings, Edinburgh, UK
    Nat Genet 24:372-6. 2000
    ....
  18. ncbi request reprint Rapid loss of Oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines
    M Buehr
    Centre for Genome Research, University of Edinburgh, Edinburgh EH9 3JQ, United Kingdom
    Biol Reprod 68:222-9. 2003
    ..Downregulation of Oct-4 appears to be a limiting factor in attempts to derive pluripotent cell lines from preimplantation embryos...
  19. ncbi request reprint Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation
    Jerome Aubert
    Institute for Stem Cell Research, University of Edinburgh, The King s Buildings, West Mains Road, Edinburgh, UK EH3 9JQ
    Nat Biotechnol 20:1240-5. 2002
    ..These findings reveal the importance of Wnt signaling in regulating ES-cell lineage diversification. More generally, this study establishes a path for rapid and direct validation of candidate genes in ES cells...
  20. ncbi request reprint Neural differentiation of mouse embryonic stem cells
    M P Stavridis
    Institute for Stem Cell Research, University of Edinburgh, The King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Biochem Soc Trans 31:45-9. 2003
    ..Here, we review these methodologies and present new tools for quantification, analysis and manipulation of embryonic stem cell neural determination...
  21. doi request reprint Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture
    Yirui Sun
    Wellcome Trust Centre for Stem Cell Research and Department of Biochemistry, University of Cambridge, Cambridge, UK
    Mol Cell Neurosci 38:245-58. 2008
    ..Human NS cells can be stably transfected to provide reporter lines and readily imaged in live monolayer cultures, creating the potential for high content genetic and chemical screens...
  22. doi request reprint The ground state of embryonic stem cell self-renewal
    Qi Long Ying
    Center for Stem Cell and Regenerative Medicine, Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, 1501 San Pablo Street, ZNI 529, Los Angeles, California 90033, USA
    Nature 453:519-23. 2008
    ..This property may account for their latent tumorigenicity. The delineation of minimal requirements for self-renewal now provides a defined platform for the precise description and dissection of the pluripotent state...
  23. ncbi request reprint Normal timing of oligodendrocyte development from genetically engineered, lineage-selectable mouse ES cells
    Nathalie Billon
    MRC Laboratory for Molecular Cell Biology and Cell Biology Unit and the Biology Department, University College London, London WC1E 6BT, UK
    J Cell Sci 115:3657-65. 2002
    ..This strategy may also be useful for producing human cells for therapy and drug screening...
  24. pmc Capturing pluripotency
    Jose Silva
    Wellcome Trust Centre for Stem Cell Research and Department of Biochemistry, University of Cambridge, Cambridge CB2 1QR, UK
    Cell 132:532-6. 2008
    ..We speculate that cell-to-cell variation may be integral to the ES cell condition, safe-guarding self-renewal while continually presenting opportunities for lineage specification...
  25. ncbi request reprint Signalling, cell cycle and pluripotency in embryonic stem cells
    Tom Burdon
    Department of Gene Expression and Development, Roslin Institute, Midlothian, EH25 9PS, Roslin, UK
    Trends Cell Biol 12:432-8. 2002
    ..We relate these observations to the unusual cell-cycle properties of ES cells and speculate on the role of the cell cycle in maintaining pluripotency...
  26. ncbi request reprint Essential alterations of heparan sulfate during the differentiation of embryonic stem cells to Sox1-enhanced green fluorescent protein-expressing neural progenitor cells
    Claire E Johnson
    Department of Medical Oncology, Cancer Research UK, The University of Manchester, Manchester, UK
    Stem Cells 25:1913-23. 2007
    ..We suggest that the stage-specific activities of these proteins are finely regulated by dynamic changes in sulfation motifs in HS chains. Disclosure of potential conflicts of interest is found at the end of this article...
  27. pmc Reprogramming efficiency following somatic cell nuclear transfer is influenced by the differentiation and methylation state of the donor nucleus
    Robert Blelloch
    Whitehead Instituteand Department of Biology, Massachusetts Institute of Technology, Cambridge, USA
    Stem Cells 24:2007-13. 2006
    ..Our results provide functional evidence that the differentiation and epigenetic state of the donor nucleus influences reprogramming efficiency...
  28. ncbi request reprint Human embryonic stem cells
    Ian Wilmut
    Science 310:1903. 2005
  29. ncbi request reprint Stem cells: Fusion brings down barriers
    Alexander Medvinsky
    Nature 422:823-5. 2003
  30. ncbi request reprint Unequal segregation of parental chromosomes in embryonic stem cell hybrids
    Natalia M Matveeva
    Institute of Cytology and Genetics, Academy of Sciences of Russia, Siberian Branch, Novosibirsk, Russia
    Mol Reprod Dev 71:305-14. 2005
    ..Nonrandom segregation implies that initial differences in the parental homologous chromosomes were not immediately equalized implying at least transient persistence of the differentiated epigenotype...