K Siddle

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. pmc The transcription factors Egr1 and Egr2 have opposing influences on adipocyte differentiation
    K B Boyle
    Department of Clinical Biochemistry, Metabolic Research Laboratories, Institute of Metabolic Science, Addenbrooke s Hospital, University of Cambridge, Cambridge, UK
    Cell Death Differ 16:782-9. 2009
  2. pmc Two isoforms of the mRNA binding protein IGF2BP2 are generated by alternative translational initiation
    Hang T T Le
    University of Cambridge Metabolic Research Laboratories and Department of Clinical Biochemistry, Institute of Metabolic Science, Addenbrooke s Hospital, Cambridge, United Kingdom
    PLoS ONE 7:e33140. 2012
  3. pmc The human insulin receptor mRNA contains a functional internal ribosome entry segment
    Keith A Spriggs
    University of Nottingham, School of Pharmacy, University Park, Nottingham NG7 2RD, UK
    Nucleic Acids Res 37:5881-93. 2009
  4. pmc Molecular basis of signaling specificity of insulin and IGF receptors: neglected corners and recent advances
    Kenneth Siddle
    University of Cambridge Metabolic Research Laboratories and Department of Clinical Biochemistry, Institute of Metabolic Science, Addenbrooke s Hospital Cambridge, UK
    Front Endocrinol (Lausanne) 3:34. 2012
  5. doi request reprint Signalling by insulin and IGF receptors: supporting acts and new players
    Kenneth Siddle
    Department of Clinical Biochemistry, Institute of Metabolic Science, Addenbrooke s Hospital, University of Cambridge Metabolic Research Laboratories, Cambridge CB2 0QQ, UK
    J Mol Endocrinol 47:R1-10. 2011
  6. ncbi request reprint Specificity in ligand binding and intracellular signalling by insulin and insulin-like growth factor receptors
    K Siddle
    University of Cambridge, Department of Clinical Biochemistry, Addenbrooke s Hospital, Cambridge CB2 2QR, UK
    Biochem Soc Trans 29:513-25. 2001
  7. ncbi request reprint Comparison of anti-apoptotic signalling by the insulin receptor and IGF-I receptor in preadipocytes and adipocytes
    B Ursø
    University of Cambridge, Department of Clinical Biochemistry, Addenbrooke s Hospital, Hills Road, CB2 2QR, Cambridge, UK
    Cell Signal 13:279-85. 2001
  8. ncbi request reprint Chloroquine extends the lifetime of the activated insulin receptor complex in endosomes
    A P Bevan
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QR, United Kingdom
    J Biol Chem 272:26833-40. 1997
  9. pmc A monoclonal anti-peptide antibody reacting with the insulin receptor beta-subunit. Characterization of the antibody and its epitope and use in immunoaffinity purification of intact receptors
    R H Ganderton
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, U K
    Biochem J 288:195-205. 1992
  10. ncbi request reprint Deletion of V335 from the L2 domain of the insulin receptor results in a conformationally abnormal receptor that is unable to bind insulin and causes Donohue's syndrome in a human subject
    S George
    Department of Clinical Biochemistry, Addenbrooke s Hospital, Cambridge, United Kingdom CB2 2QQ
    Endocrinology 144:631-7. 2003

Collaborators

Detail Information

Publications25

  1. pmc The transcription factors Egr1 and Egr2 have opposing influences on adipocyte differentiation
    K B Boyle
    Department of Clinical Biochemistry, Metabolic Research Laboratories, Institute of Metabolic Science, Addenbrooke s Hospital, University of Cambridge, Cambridge, UK
    Cell Death Differ 16:782-9. 2009
    ..Further, the pro-differentiation effects of IBMX involve suppression of the inhibitory influence of Egr1...
  2. pmc Two isoforms of the mRNA binding protein IGF2BP2 are generated by alternative translational initiation
    Hang T T Le
    University of Cambridge Metabolic Research Laboratories and Department of Clinical Biochemistry, Institute of Metabolic Science, Addenbrooke s Hospital, Cambridge, United Kingdom
    PLoS ONE 7:e33140. 2012
    ..We conclude that structural heterogeneity of IGF2BP2 protein should be taken into account when considering cellular function...
  3. pmc The human insulin receptor mRNA contains a functional internal ribosome entry segment
    Keith A Spriggs
    University of Nottingham, School of Pharmacy, University Park, Nottingham NG7 2RD, UK
    Nucleic Acids Res 37:5881-93. 2009
    ..IRES activity was stimulated by insulin in sub-confluent cells. The IRES may function to maintain expression of IR protein in tissues such as the brain where mRNA translation by cap-dependent scanning is less effective...
  4. pmc Molecular basis of signaling specificity of insulin and IGF receptors: neglected corners and recent advances
    Kenneth Siddle
    University of Cambridge Metabolic Research Laboratories and Department of Clinical Biochemistry, Institute of Metabolic Science, Addenbrooke s Hospital Cambridge, UK
    Front Endocrinol (Lausanne) 3:34. 2012
    ..Cellular context, rather than intrinsic receptor activity, therefore appears to be the major determinant of whether responses to insulin and IGFs are perceived as "metabolic" or "mitogenic."..
  5. doi request reprint Signalling by insulin and IGF receptors: supporting acts and new players
    Kenneth Siddle
    Department of Clinical Biochemistry, Institute of Metabolic Science, Addenbrooke s Hospital, University of Cambridge Metabolic Research Laboratories, Cambridge CB2 0QQ, UK
    J Mol Endocrinol 47:R1-10. 2011
    ..This review will focus on recent studies that have extended our understanding of insulin/IGF signalling pathways, and the elements that may contribute to specificity...
  6. ncbi request reprint Specificity in ligand binding and intracellular signalling by insulin and insulin-like growth factor receptors
    K Siddle
    University of Cambridge, Department of Clinical Biochemistry, Addenbrooke s Hospital, Cambridge CB2 2QR, UK
    Biochem Soc Trans 29:513-25. 2001
    ..Expression and activation of these chimaeras in cell lines reveals subtle differences in signalling and end-point responses, which may depend on cell background...
  7. ncbi request reprint Comparison of anti-apoptotic signalling by the insulin receptor and IGF-I receptor in preadipocytes and adipocytes
    B Ursø
    University of Cambridge, Department of Clinical Biochemistry, Addenbrooke s Hospital, Hills Road, CB2 2QR, Cambridge, UK
    Cell Signal 13:279-85. 2001
    ..We conclude that IR and IGFR have equal potential to inhibit apoptosis in cell backgrounds, which are normally responsive to either IGF-I or insulin...
  8. ncbi request reprint Chloroquine extends the lifetime of the activated insulin receptor complex in endosomes
    A P Bevan
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QR, United Kingdom
    J Biol Chem 272:26833-40. 1997
    ....
  9. pmc A monoclonal anti-peptide antibody reacting with the insulin receptor beta-subunit. Characterization of the antibody and its epitope and use in immunoaffinity purification of intact receptors
    R H Ganderton
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, U K
    Biochem J 288:195-205. 1992
    ..This approach to purification under very mild conditions may in principle be used with any protein for which an antibody is available and for which a peptide epitope or 'mimotope' can be identified...
  10. ncbi request reprint Deletion of V335 from the L2 domain of the insulin receptor results in a conformationally abnormal receptor that is unable to bind insulin and causes Donohue's syndrome in a human subject
    S George
    Department of Clinical Biochemistry, Addenbrooke s Hospital, Cambridge, United Kingdom CB2 2QQ
    Endocrinology 144:631-7. 2003
    ..The severe biochemical and clinical consequences of this novel mutation, which occur despite substantial expression on the cell surface, emphasize the crucial role of the L2 domain in ligand binding by the insulin receptor...
  11. ncbi request reprint Immunological studies of type I IGF receptors and insulin receptors: characterisation of hybrid and atypical receptor subtypes
    M A Soos
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, U K
    Adv Exp Med Biol 343:145-57. 1993
  12. pmc Anti-(insulin receptor) monoclonal antibody-stimulated tyrosine phosphorylation in cells transfected with human insulin receptor cDNA
    N P Brindle
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, U K
    Biochem J 268:615-20. 1990
    ..These data are consistent with a role for the insulin receptor tyrosine kinase activity in the mechanism of action of insulin-mimetic anti-receptor antibodies...
  13. pmc Monoclonal antibodies to the insulin receptor mimic metabolic effects of insulin but do not stimulate receptor autophosphorylation in transfected NIH 3T3 fibroblasts
    M A Soos
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, United Kingdom
    Proc Natl Acad Sci U S A 86:5217-21. 1989
    ....
  14. ncbi request reprint Tumor necrosis factor-alpha induces apoptosis of human adipose cells
    J B Prins
    Department of Medicine, University of Cambridge, Addenbrooke s Hospital, England, U K
    Diabetes 46:1939-44. 1997
    ....
  15. ncbi request reprint Defect in insulin-like growth factor-1 survival mechanism in atherosclerotic plaque-derived vascular smooth muscle cells is mediated by reduced surface binding and signaling
    V A Patel
    Departments of Medicine, Addenbrooke's Hospital, Cambridge, UK
    Circ Res 88:895-902. 2001
    ..This impaired IGF-1 protection against apoptosis may promote VSMC loss and plaque instability in atherosclerosis...
  16. ncbi request reprint A panel of monoclonal antibodies for the type I insulin-like growth factor receptor. Epitope mapping, effects on ligand binding, and biological activity
    M A Soos
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, United Kingdom
    J Biol Chem 267:12955-63. 1992
    ..These antibodies promise to be valuable tools in resolving issues of IGF-I receptor heterogeneity and in studying the structure and function of classical type I receptors and insulin/IGF receptor hybrids...
  17. ncbi request reprint Hybrid and atypical insulin/insulin-like growth factor I receptors
    K Siddle
    Department of Clinical Biochemistry, Addenbrooke's Hospital, University of Cambridge, UK
    Horm Res 41:56-64; discussion 65. 1994
    ....
  18. ncbi request reprint Two naturally occurring insulin receptor tyrosine kinase domain mutants provide evidence that phosphoinositide 3-kinase activation alone is not sufficient for the mediation of insulin's metabolic and mitogenic effects
    A Krook
    Department of Medicine, Cambridge University, Addenbrookes Hospital, Hills Road, CB2 2QR, Cambridge, United Kingdom
    J Biol Chem 272:30208-14. 1997
    ..Additionally, the data provide support for the notion that insulin activation of Ras is more closely linked with Shc, and not IRS-1, phosphorylation...
  19. pmc Immunological relationships between receptors for insulin and insulin-like growth factor I. Evidence for structural heterogeneity of insulin-like growth factor I receptors involving hybrids with insulin receptors
    M A Soos
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, U K
    Biochem J 263:553-63. 1989
    ....
  20. pmc Insulin receptor/IGF-I receptor hybrids are widely distributed in mammalian tissues: quantification of individual receptor species by selective immunoprecipitation and immunoblotting
    E M Bailyes
    University of Cambridge, Department of Clinical Biochemistry, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QR, U K
    Biochem J 327:209-15. 1997
    ..However, in placenta the proportions of IR, IGFR and hybrids were consistent with a process of random assembly reflecting the molar ratio of IR and IGFR half-receptors...
  21. ncbi request reprint The role of insulin dissociation from its endosomal receptor in insulin degradation
    A P Bevan
    Department of Clinical Biochemistry, University of Cambridge, UK
    Mol Cell Endocrinol 164:145-57. 2000
    ....
  22. ncbi request reprint Identification of epitopes on the human insulin receptor reacting with rabbit polyclonal antisera and mouse monoclonal antibodies
    S A Prigent
    Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, United Kingdom
    J Biol Chem 265:9970-7. 1990
    ..The identification of epitopes therefore provides information on receptor conformation and allows structural domains to be identified which are involved in the functional effects of different antibodies...
  23. ncbi request reprint Adipose depot-specific expression of cIAP2 in human preadipocytes and modulation of expression by serum factors and TNFalpha
    C U Niesler
    University of Cambridge, Department of Clinical Biochemistry, Addenbrooke s Hospital, Cambridge, UK
    Int J Obes Relat Metab Disord 25:1027-33. 2001
    ..We have previously demonstrated that cIAP2 mRNA is significantly overexpressed in omental (Om) compared with subcutaneous (Sc) adipocytes. This molecule is involved in the TNFalpha signalling pathway and may inhibit apoptosis...
  24. pmc Biosynthesis of insulin secretory granule membrane proteins. Control by glucose
    K A Grimaldi
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, U K
    Biochem J 245:567-73. 1987
    ..Factors which acutely control insulin secretion may also control granule biogenesis, although the two processes are not coupled in an obligatory fashion...
  25. pmc Purified hybrid insulin/insulin-like growth factor-I receptors bind insulin-like growth factor-I, but not insulin, with high affinity
    M A Soos
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, U K
    Biochem J 290:419-26. 1993
    ....