Alex J Shortt

Summary

Affiliation: University College London
Country: UK

Publications

  1. ncbi request reprint Characterization of the limbal epithelial stem cell niche: novel imaging techniques permit in vivo observation and targeted biopsy of limbal epithelial stem cells
    Alex J Shortt
    MRC Clinical Research Fellow, Institute of Ophthalmology and Moorfields Eye Hospital, 11 43 Bath Street, London, EC1V 9EL, United Kingdom
    Stem Cells 25:1402-9. 2007
  2. ncbi request reprint Transplantation of ex vivo cultured limbal epithelial stem cells: a review of techniques and clinical results
    Alex J Shortt
    Ocular Repair and Regeneration Biology Unit, Institute of Ophthalmology, London
    Surv Ophthalmol 52:483-502. 2007
  3. ncbi request reprint Ex vivo cultured limbal epithelial transplantation. A clinical perspective
    Alex J Shortt
    Cells for Sight Transplantation and Research Programme, Department of Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, London, United Kingdom
    Ocul Surf 8:80-90. 2010
  4. doi request reprint Laser-assisted in-situ keratomileusis (LASIK) versus photorefractive keratectomy (PRK) for myopia
    Alex J Shortt
    The Moorfields Eye Hospital UCL Institute of Ophthalmology National Institute for Health Research Biomedical Research Centre, London, UK
    Cochrane Database Syst Rev 1:CD005135. 2013
  5. ncbi request reprint Evidence for superior efficacy and safety of LASIK over photorefractive keratectomy for correction of myopia
    Alex J Shortt
    Moorfields Eye Hospital, London, United Kingdom
    Ophthalmology 113:1897-908. 2006
  6. doi request reprint Corneal stem cells in the eye clinic
    Alex J Shortt
    Department of Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, 11 43 Bath Street, London EC1V 9EL, UK
    Br Med Bull 100:209-25. 2011
  7. doi request reprint The effect of amniotic membrane preparation method on its ability to serve as a substrate for the ex-vivo expansion of limbal epithelial cells
    Alex J Shortt
    Cells for Sight Transplantation and Research Programme, United Kingdom
    Biomaterials 30:1056-65. 2009
  8. doi request reprint Ex vivo expansion and transplantation of limbal epithelial stem cells
    Alex J Shortt
    Cells for Sight Transplantation and Research Programme, UCL Institute of Ophthalmology, London, United Kingdom
    Ophthalmology 115:1989-97. 2008
  9. ncbi request reprint Stem cell therapy delivery: treading the regulatory tightrope
    Julie T Daniels
    Institute of Ophthalmology, University College London, London, UK
    Regen Med 1:715-9. 2006
  10. doi request reprint IL6 and the human limbal stem cell niche: a mediator of epithelial-stromal interaction
    Maria Notara
    Department of Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, London, UK
    Stem Cell Res 5:188-200. 2010

Detail Information

Publications15

  1. ncbi request reprint Characterization of the limbal epithelial stem cell niche: novel imaging techniques permit in vivo observation and targeted biopsy of limbal epithelial stem cells
    Alex J Shortt
    MRC Clinical Research Fellow, Institute of Ophthalmology and Moorfields Eye Hospital, 11 43 Bath Street, London, EC1V 9EL, United Kingdom
    Stem Cells 25:1402-9. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  2. ncbi request reprint Transplantation of ex vivo cultured limbal epithelial stem cells: a review of techniques and clinical results
    Alex J Shortt
    Ocular Repair and Regeneration Biology Unit, Institute of Ophthalmology, London
    Surv Ophthalmol 52:483-502. 2007
    ..Nonetheless, we conclude that there is sufficient evidence to support the continued use and refinement of this procedure as a treatment for corneal stem cell deficiency...
  3. ncbi request reprint Ex vivo cultured limbal epithelial transplantation. A clinical perspective
    Alex J Shortt
    Cells for Sight Transplantation and Research Programme, Department of Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, London, United Kingdom
    Ocul Surf 8:80-90. 2010
    ..Other tissues, such as oral mucosal epithelium, are emerging as viable alternative sources of cells, especially for patients with bilateral disease...
  4. doi request reprint Laser-assisted in-situ keratomileusis (LASIK) versus photorefractive keratectomy (PRK) for myopia
    Alex J Shortt
    The Moorfields Eye Hospital UCL Institute of Ophthalmology National Institute for Health Research Biomedical Research Centre, London, UK
    Cochrane Database Syst Rev 1:CD005135. 2013
    ..The two most commonly used surgical techniques to permanently correct myopia are photorefractive keratectomy (PRK) and laser-assisted in-situ keratomileusis (LASIK)...
  5. ncbi request reprint Evidence for superior efficacy and safety of LASIK over photorefractive keratectomy for correction of myopia
    Alex J Shortt
    Moorfields Eye Hospital, London, United Kingdom
    Ophthalmology 113:1897-908. 2006
    ..To examine possible differences in efficacy and safety between LASIK and photorefractive keratectomy (PRK) for correction of myopia...
  6. doi request reprint Corneal stem cells in the eye clinic
    Alex J Shortt
    Department of Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, 11 43 Bath Street, London EC1V 9EL, UK
    Br Med Bull 100:209-25. 2011
    ..The majority of cases result from ulceration and scarring following infection or trauma, but in a proportion corneal epithelial stem cell (SC) deficiency leads to an inability to maintain a healthy corneal surface...
  7. doi request reprint The effect of amniotic membrane preparation method on its ability to serve as a substrate for the ex-vivo expansion of limbal epithelial cells
    Alex J Shortt
    Cells for Sight Transplantation and Research Programme, United Kingdom
    Biomaterials 30:1056-65. 2009
    ..The use of glycerol as a cryoprotectant impairs this ability whereas simple frozen HAM appears to work extremely well for this purpose...
  8. doi request reprint Ex vivo expansion and transplantation of limbal epithelial stem cells
    Alex J Shortt
    Cells for Sight Transplantation and Research Programme, UCL Institute of Ophthalmology, London, United Kingdom
    Ophthalmology 115:1989-97. 2008
    ..To determine, using objective measures, the outcome of ex vivo cultured limbal epithelial stem cell (LESC) transplantation performed in compliance with good manufacturing practice using a novel culture system without 3T3 feeder cells...
  9. ncbi request reprint Stem cell therapy delivery: treading the regulatory tightrope
    Julie T Daniels
    Institute of Ophthalmology, University College London, London, UK
    Regen Med 1:715-9. 2006
    ..This may help colleagues who are developing innovative academic research-driven stem cell therapies regarding donor consent, raw materials, quality assurance, laboratory specification, indemnity and funding...
  10. doi request reprint IL6 and the human limbal stem cell niche: a mediator of epithelial-stromal interaction
    Maria Notara
    Department of Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, London, UK
    Stem Cell Res 5:188-200. 2010
    ..STAT3 and IL6 inhibition in 3.1SF cultures significantly reduced HLE colony-forming efficiency, suggesting a previously undetected STAT3-mediated involvement of IL6 in the maintenance of HLE cells in a progenitor-like state...
  11. doi request reprint Three-year outcomes of cultured limbal epithelial allografts in aniridia and stevens-johnson syndrome evaluated using the clinical outcome assessment in surgical trials assessment tool
    Alex J Shortt
    Cells for Sight Transplantation and Research Programme and Ocular Biology and Therapeutics Division, University College London Institute of Ophthalmology, London, United Kingdom Moorfields Eye Hospital National Health Service Foundation Trust, London, United Kingdom University College London Clinical Trials Unit, London, United Kingdom
    Stem Cells Transl Med 3:265-75. 2014
    ..The COASTL tool showed that following allo-CLET there was a decrease in LSCD severity and an increase in visual acuity up to 12 months post-treatment, but thereafter LSCD severity and visual acuity progressively deteriorated. ..
  12. ncbi request reprint Limbal epithelial stem cell therapy
    Julie T Daniels
    UCL Institute of Ophthalmology, Cells for Sight Transplantation and Research Programme, Ocular Repair and Regeneration Biology Unit, Division of Pathology, London, EC1V 9EL, UK
    Expert Opin Biol Ther 7:1-3. 2007
    ....
  13. pmc Plastic compressed collagen as a novel carrier for expanded human corneal endothelial cells for transplantation
    Hannah J Levis
    Department of Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, London, UK
    PLoS ONE 7:e50993. 2012
    ..This method provides expanded endothelial cells with a substrate that could be suitable for transplantation allowing one donor cornea to potentially treat multiple patients...
  14. ncbi request reprint TGFbeta stimulated re-epithelialisation is regulated by CTGF and Ras/MEK/ERK signalling
    Genevieve A Secker
    Department of Pathology, Cells for Sight Transplantation and Research Programme, UCL Institute of Ophthalmology, 11 43 Bath Street, London, EC1V 9EL, UK
    Exp Cell Res 314:131-42. 2008
    ..These data reveal a novel function for CTGF in the regulation of epithelial tissue repair beyond its established role in fibrosis, and further highlight the complexity of TGFbeta regulation of epithelial cell function...
  15. ncbi request reprint Pars plana lensectomy for the management of ectopia lentis in children
    Alex J Shortt
    National Childrens Eye Centre, Childrens University Hospital, Dublin, Ireland
    J Pediatr Ophthalmol Strabismus 41:289-94. 2004
    ..It may also occur as an isolated abnormality in an otherwise healthy individual. Surgical intervention is necessary when lens subluxation causes a significant refractive error resulting in amblyopia...