David J Sherratt

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. pmc Separating speed and ability to displace roadblocks during DNA translocation by FtsK
    Estelle Crozat
    Department of Biochemistry, University of Oxford, Oxford, UK
    EMBO J 29:1423-33. 2010
  2. pmc The SMC complex MukBEF recruits topoisomerase IV to the origin of replication region in live Escherichia coli
    Emilien Nicolas
    Department of Biochemistry, University of Oxford, Oxford, United Kingdom
    MBio 5:e01001-13. 2014
  3. pmc Replication-directed sister chromosome alignment in Escherichia coli
    Xun Liu
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Mol Microbiol 75:1090-7. 2010
  4. pmc The N-terminal membrane-spanning domain of the Escherichia coli DNA translocase FtsK hexamerizes at midcell
    Paola Bisicchia
    Department of Biochemistry
    MBio 4:e00800-13. 2013
  5. pmc MinC, MinD, and MinE drive counter-oscillation of early-cell-division proteins prior to Escherichia coli septum formation
    Paola Bisicchia
    Department of Biochemistry, University of Oxford, Oxford, United Kingdom
    MBio 4:e00856-13. 2013
  6. doi request reprint The Escherichia coli DNA translocase FtsK
    David J Sherratt
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Biochem Soc Trans 38:395-8. 2010
  7. pmc Conformational transitions during FtsK translocase activation of individual XerCD-dif recombination complexes
    Pawel Zawadzki
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 110:17302-7. 2013
  8. pmc The FtsK gamma domain directs oriented DNA translocation by interacting with KOPS
    Viknesh Sivanathan
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Nat Struct Mol Biol 13:965-72. 2006
  9. pmc Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase
    James Yates
    Division of Molecular Genetics, Department Biochemistry, University of Oxford, UK
    Mol Microbiol 59:1754-66. 2006
  10. pmc Decatenation of DNA circles by FtsK-dependent Xer site-specific recombination
    Stephen C Y Ip
    University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    EMBO J 22:6399-407. 2003

Collaborators

Detail Information

Publications44

  1. pmc Separating speed and ability to displace roadblocks during DNA translocation by FtsK
    Estelle Crozat
    Department of Biochemistry, University of Oxford, Oxford, UK
    EMBO J 29:1423-33. 2010
    ..This separation of translocation velocity and ability to displace roadblocks is more consistent with a sequential escort mechanism than stochastic, hand-off, or concerted mechanisms...
  2. pmc The SMC complex MukBEF recruits topoisomerase IV to the origin of replication region in live Escherichia coli
    Emilien Nicolas
    Department of Biochemistry, University of Oxford, Oxford, United Kingdom
    MBio 5:e01001-13. 2014
    ..Taken together, the data implicate MukBEF as a key component of the DNA segregation process by acting in concert with TopoIV to promote decatenation and positioning of newly replicated oris...
  3. pmc Replication-directed sister chromosome alignment in Escherichia coli
    Xun Liu
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Mol Microbiol 75:1090-7. 2010
    ....
  4. pmc The N-terminal membrane-spanning domain of the Escherichia coli DNA translocase FtsK hexamerizes at midcell
    Paola Bisicchia
    Department of Biochemistry
    MBio 4:e00800-13. 2013
    ..The hexameric state of the FtsK N-terminal domain at the division site may facilitate assembly of a functional C-terminal DNA translocase on chromosomal DNA...
  5. pmc MinC, MinD, and MinE drive counter-oscillation of early-cell-division proteins prior to Escherichia coli septum formation
    Paola Bisicchia
    Department of Biochemistry, University of Oxford, Oxford, United Kingdom
    MBio 4:e00856-13. 2013
    ..Reconstitution of FtsZ-Min waves on lipid bilayers shows that FtsZ bundles partition away from high concentrations of MinC and that ZapA appears to protect FtsZ from MinC by inhibiting FtsZ turnover...
  6. doi request reprint The Escherichia coli DNA translocase FtsK
    David J Sherratt
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Biochem Soc Trans 38:395-8. 2010
    ..In the present paper, the properties in vivo and in vitro of FtsK and its relatives are discussed in relation to the biological functions of these remarkable enzymes...
  7. pmc Conformational transitions during FtsK translocase activation of individual XerCD-dif recombination complexes
    Pawel Zawadzki
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 110:17302-7. 2013
    ..These observations, along with the calculated intermediate lifetimes, inform the reaction mechanism, which plays a key role in chromosome unlinking in most bacteria with circular chromosomes. ..
  8. pmc The FtsK gamma domain directs oriented DNA translocation by interacting with KOPS
    Viknesh Sivanathan
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Nat Struct Mol Biol 13:965-72. 2006
    ....
  9. pmc Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase
    James Yates
    Division of Molecular Genetics, Department Biochemistry, University of Oxford, UK
    Mol Microbiol 59:1754-66. 2006
    ....
  10. pmc Decatenation of DNA circles by FtsK-dependent Xer site-specific recombination
    Stephen C Y Ip
    University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    EMBO J 22:6399-407. 2003
    ..We propose that the FtsK-XerCD recombination machinery, which converts chromosomal dimers to monomers, may also function in vivo in removing the final catenation links remaining upon completion of DNA replication...
  11. pmc FtsK translocation on DNA stops at XerCD-dif
    James E Graham
    Department of Biochemistry, University of Oxford, Oxford, UK
    Nucleic Acids Res 38:72-81. 2010
    ....
  12. pmc KOPS-guided DNA translocation by FtsK safeguards Escherichia coli chromosome segregation
    Viknesh Sivanathan
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Mol Microbiol 71:1031-42. 2009
    ..For example, when Cre-loxP recombination replaces XerCD-dif recombination in dimer resolution, when functional MukBEF is absent, or when replication terminates away from ter...
  13. ncbi request reprint Double-stranded DNA translocation: structure and mechanism of hexameric FtsK
    Thomas H Massey
    Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Mol Cell 23:457-69. 2006
    ..Comparison of FtsK monomer structures from two different crystal forms highlights a conformational change that we propose is the structural basis for a rotary inchworm mechanism of DNA translocation...
  14. pmc Unlinking chromosome catenanes in vivo by site-specific recombination
    Ian Grainge
    Department of Biochemistry, University of Oxford, Oxford, UK
    EMBO J 26:4228-38. 2007
    ..We conclude that FtsK acts in vivo to simplify chromosomal topology as Xer recombination interconverts monomeric and dimeric chromosomes...
  15. pmc Dancing around the divisome: asymmetric chromosome segregation in Escherichia coli
    Xindan Wang
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    Genes Dev 19:2367-77. 2005
    ..As ter duplicates at mid-cell, sister nucleoid separation appears complete. After initiation of invagination, the FtsZ ring disassembles, leaving FtsK to complete chromosome segregation and cytokinesis...
  16. pmc Capturing reaction paths and intermediates in Cre-loxP recombination using single-molecule fluorescence
    Justin N M Pinkney
    Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford OX1 3PU, United Kingdom
    Proc Natl Acad Sci U S A 109:20871-6. 2012
    ..After recombination, the product synaptic complex was extremely stable and refractory to subsequent rounds of recombination...
  17. pmc Tracking of controlled Escherichia coli replication fork stalling and restart at repressor-bound DNA in vivo
    Christophe Possoz
    Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford, UK
    EMBO J 25:2596-604. 2006
    ..Roadblocks positioned near oriC or the dif site did not prevent replication and segregation of the rest of the chromosome...
  18. doi request reprint Chromosome replication and segregation in bacteria
    Rodrigo Reyes-Lamothe
    Department of Biochemistry, University of Oxford, OX1 3QU, Oxford, United Kingdom
    Annu Rev Genet 46:121-43. 2012
    ..We also describe what is known about the three conserved families of ATP-binding proteins that contribute to chromosome segregation and discuss their inter-relationships in a range of disparate bacteria...
  19. pmc Recombination and chromosome segregation
    David J Sherratt
    Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Philos Trans R Soc Lond B Biol Sci 359:61-9. 2004
    ....
  20. pmc Asymmetric activation of Xer site-specific recombination by FtsK
    Thomas H Massey
    Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    EMBO Rep 5:399-404. 2004
    ..Only one such DNA extension is required. Taken together, our data suggest that FtsK needs to contact the XerD recombinase to switch its activity on using ATP hydrolysis...
  21. pmc In vivo architecture and action of bacterial structural maintenance of chromosome proteins
    Anjana Badrinarayanan
    Department of Biochemistry, University of Oxford, UK
    Science 338:528-31. 2012
    ..Thus, by functioning in pairs, MukBEF complexes may undergo multiple cycles of ATP hydrolysis without being released from DNA, analogous to the behavior of well-characterized molecular motors...
  22. pmc Independent positioning and action of Escherichia coli replisomes in live cells
    Rodrigo Reyes-Lamothe
    Department of Biochemistry, University of Oxford, Oxford OX1 3 QU, UK
    Cell 133:90-102. 2008
    ..We conclude that independent replication forks follow the path of the compacted chromosomal DNA, with no structure other than DNA anchoring the replisome to any particular cellular region...
  23. pmc The two Escherichia coli chromosome arms locate to separate cell halves
    Xindan Wang
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    Genes Dev 20:1727-31. 2006
    ....
  24. pmc High-copy bacterial plasmids diffuse in the nucleoid-free space, replicate stochastically and are randomly partitioned at cell division
    Rodrigo Reyes-Lamothe
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK, Department of Biology, McGill University, Montreal, Quebec H3G 0B1, Canada and Department of Biological Science, Center for Applied Biotechnology Studies, College of Natural Science and Mathematics, California State University Fullerton, Fullerton, CA 92834 6850, USA
    Nucleic Acids Res 42:1042-51. 2014
    ..Complete replication of individual molecules occurred stochastically and independently in the nucleoid-free space throughout the cell cycle, with a constant probability of initiation per plasmid. ..
  25. ncbi request reprint Functional analysis of the C-terminal domains of the site-specific recombinases XerC and XerD
    Henrique Ferreira
    Division of Molecular Genetics, Biochemistry Department, University of Oxford, UK
    J Mol Biol 330:15-27. 2003
    ....
  26. ncbi request reprint Species specificity in the activation of Xer recombination at dif by FtsK
    James Yates
    Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Mol Microbiol 49:241-9. 2003
    ..We mapped the region of FtsK implicated in species specificity to the extreme 140-amino-acid C-terminal residues of the protein. Our results suggest that FtsK interacts directly with XerCD in order to activate recombination at dif...
  27. pmc Activation of XerCD-dif recombination by the FtsK DNA translocase
    Ian Grainge
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Nucleic Acids Res 39:5140-8. 2011
    ....
  28. pmc Stoichiometry and architecture of active DNA replication machinery in Escherichia coli
    Rodrigo Reyes-Lamothe
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Science 328:498-501. 2010
    ..Single-strand binding protein has a broader spatial distribution than the core components, with 5 to 11 tetramers per replisome. This in vivo technique could provide single-molecule insight into other molecular machines...
  29. pmc Single-molecule DNA repair in live bacteria
    Stephan Uphoff
    Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford OX1 3PU, United Kingdom
    Proc Natl Acad Sci U S A 110:8063-8. 2013
    ..We integrated these single-molecule observations to generate a quantitative, systems-level description of a model repair pathway in vivo...
  30. ncbi request reprint Bacterial chromosome dynamics
    David J Sherratt
    Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Science 301:780-5. 2003
    ..Bacteria specify positional information, which determines where cell division will occur and which places the replication machinery and chromosomal loci at defined locations that change during cell cycle progression...
  31. pmc mwr Xer site-specific recombination is hypersensitive to DNA supercoiling
    Sonia Trigueros
    Bionanotechnology IRC Department of Physics, University of Oxford, Oxford OX1 3QU, UK
    Nucleic Acids Res 37:3580-7. 2009
    ....
  32. pmc A streptavidin variant with slower biotin dissociation and increased mechanostability
    Claire E Chivers
    Department of Biochemistry, Oxford University, UK
    Nat Methods 7:391-3. 2010
    ..FtsK, a motor protein important in chromosome segregation, rapidly displaced streptavidin from biotinylated DNA, whereas traptavidin resisted displacement, indicating the force generated by Ftsk translocation...
  33. pmc RecA bundles mediate homology pairing between distant sisters during DNA break repair
    Christian Lesterlin
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Nature 506:249-53. 2014
    ..This work reveals an unanticipated role of RecA bundles in channelling the movement of the DNA DSB ends, thereby facilitating the long-range homology search that occurs before the strand invasion and transfer reactions. ..
  34. ncbi request reprint Spatial and temporal organization of replicating Escherichia coli chromosomes
    Ivy F Lau
    Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Mol Microbiol 49:731-43. 2003
    ..This asymmetry could provide a mechanism by which the chromosome segregation protein FtsK, located at the division septum, can act directionally to ensure that the septal region is free of DNA before the completion of cell division...
  35. pmc Accessory factors determine the order of strand exchange in Xer recombination at psi
    Migena Bregu
    Microbiology Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    EMBO J 21:3888-97. 2002
    ..This finding has important implications for the way in which accessory proteins interact with the recombinases...
  36. pmc Modulation of Escherichia coli sister chromosome cohesion by topoisomerase IV
    Xindan Wang
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    Genes Dev 22:2426-33. 2008
    ..Therefore, we propose that precatenanes, which form as replication progresses by interwinding of newly replicated sister chromosomes, are responsible for E. coli sister chromosome cohesion...
  37. pmc Independent segregation of the two arms of the Escherichia coli ori region requires neither RNA synthesis nor MreB dynamics
    Xindan Wang
    Department of Biochemistry, University of Oxford, South Parks Rd, Oxford, United Kingdom
    J Bacteriol 192:6143-53. 2010
    ..Inhibition of RNA synthesis and inhibition of the dynamic polymerization of the actin homolog MreB did not affect ori and bulk chromosome segregation...
  38. doi request reprint Visualizing genetic loci and molecular machines in living bacteria
    Xindan Wang
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford, UK
    Biochem Soc Trans 36:749-53. 2008
    ....
  39. ncbi request reprint Engineering a 2D protein-DNA crystal
    Jonathan Malo
    Department of Physics, Clarendon Laboratory, University of Oxford, Parks Road, Oxford OX1 3PU, UK
    Angew Chem Int Ed Engl 44:3057-61. 2005
  40. pmc Sequence-specific assembly of FtsK hexamers establishes directional translocation on DNA
    James E Graham
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 107:20263-8. 2010
    ..We also estimate the ATP coupling efficiency of translocation to be 1.63-2.11 bp of dsDNA translocated/ATP hydrolyzed. The data were used to derive a model for the assembly, initiation, and translocation of FtsK hexamers...
  41. pmc Replication and segregation of an Escherichia coli chromosome with two replication origins
    Xindan Wang
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 108:E243-50. 2011
    ..In all strains, spatial separation of sister loci adjacent to active origins occurred shortly after their replication, independently of whether replication initiated at the normal origin, the ectopic origin, or both origins...
  42. pmc fpr, a deficient Xer recombination site from a Salmonella plasmid, fails to confer stability by dimer resolution: comparative studies with the pJHCMW1 mwr site
    Tung Tran
    Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    J Bacteriol 192:883-7. 2010
    ..Mutagenesis and comparative studies with mwr, a site closely related to fpr, indicate that there is an interdependence of the sequences in the XerC binding region and the central region in Xer site-specific recombination sites...
  43. pmc Hydroxy-terminated conjugated polymer nanoparticles have near-unity bright fraction and reveal cholesterol-dependence of IGF1R nanodomains
    Apurba L Koner
    Department of Biochemistry, Oxford University, South Parks Road, OX1 3QU, United Kingdom
    ACS Nano 7:1137-44. 2013
    ..The near-unity bright fraction and low nonspecific binding of hydroxy-Pdots, combined with Pdot photostability and lack of blinking, provides many advantages for investigations at the single molecule level...
  44. pmc Osmoregulation of dimer resolution at the plasmid pJHCMW1 mwr locus by Escherichia coli XerCD recombination
    Huong Pham
    Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    J Bacteriol 184:1607-16. 2002
    ..coli ARG box consensus. The central region of the mwr core recombination site plays a role in regulation of site-specific recombination by the osmotic pressure of the medium...