Research Topics
| David J SherrattSummaryAffiliation: University of Oxford Country: UK Publications
| Collaborators
|
Detail Information
Publications
Chromosome replication and segregation in bacteriaRodrigo Reyes-Lamothe
Department of Biochemistry, University of Oxford, OX1 3QU, Oxford, United Kingdom
Annu Rev Genet 46:121-43. 2012..We also describe what is known about the three conserved families of ATP-binding proteins that contribute to chromosome segregation and discuss their inter-relationships in a range of disparate bacteria...- The Escherichia coli DNA translocase FtsKDavid J Sherratt
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
Biochem Soc Trans 38:395-8. 2010..In the present paper, the properties in vivo and in vitro of FtsK and its relatives are discussed in relation to the biological functions of these remarkable enzymes... 
Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinaseJames Yates
Division of Molecular Genetics, Department Biochemistry, University of Oxford, UK
Mol Microbiol 59:1754-66. 2006....- The FtsK gamma domain directs oriented DNA translocation by interacting with KOPSViknesh Sivanathan
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
Nat Struct Mol Biol 13:965-72. 2006.... - Decatenation of DNA circles by FtsK-dependent Xer site-specific recombinationStephen C Y Ip
University of Oxford, South Parks Road, Oxford OX1 3QU, UK
EMBO J 22:6399-407. 2003..We propose that the FtsK-XerCD recombination machinery, which converts chromosomal dimers to monomers, may also function in vivo in removing the final catenation links remaining upon completion of DNA replication... - Unlinking chromosome catenanes in vivo by site-specific recombinationIan Grainge
Department of Biochemistry, University of Oxford, Oxford, UK
EMBO J 26:4228-38. 2007..We conclude that FtsK acts in vivo to simplify chromosomal topology as Xer recombination interconverts monomeric and dimeric chromosomes... - FtsK translocation on DNA stops at XerCD-difJames E Graham
Department of Biochemistry, University of Oxford, Oxford, UK
Nucleic Acids Res 38:72-81. 2010.... 
Double-stranded DNA translocation: structure and mechanism of hexameric FtsKThomas H Massey
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
Mol Cell 23:457-69. 2006..Comparison of FtsK monomer structures from two different crystal forms highlights a conformational change that we propose is the structural basis for a rotary inchworm mechanism of DNA translocation...- KOPS-guided DNA translocation by FtsK safeguards Escherichia coli chromosome segregationViknesh Sivanathan
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
Mol Microbiol 71:1031-42. 2009..For example, when Cre-loxP recombination replaces XerCD-dif recombination in dimer resolution, when functional MukBEF is absent, or when replication terminates away from ter... - Tracking of controlled Escherichia coli replication fork stalling and restart at repressor-bound DNA in vivoChristophe Possoz
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford, UK
EMBO J 25:2596-604. 2006..Roadblocks positioned near oriC or the dif site did not prevent replication and segregation of the rest of the chromosome... - Dancing around the divisome: asymmetric chromosome segregation in Escherichia coliXindan Wang
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
Genes Dev 19:2367-77. 2005..As ter duplicates at mid-cell, sister nucleoid separation appears complete. After initiation of invagination, the FtsZ ring disassembles, leaving FtsK to complete chromosome segregation and cytokinesis... - Recombination and chromosome segregationDavid J Sherratt
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
Philos Trans R Soc Lond B Biol Sci 359:61-9. 2004.... - Asymmetric activation of Xer site-specific recombination by FtsKThomas H Massey
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
EMBO Rep 5:399-404. 2004..Only one such DNA extension is required. Taken together, our data suggest that FtsK needs to contact the XerD recombinase to switch its activity on using ATP hydrolysis... - Species specificity in the activation of Xer recombination at dif by FtsKJames Yates
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Mol Microbiol 49:241-9. 2003..We mapped the region of FtsK implicated in species specificity to the extreme 140-amino-acid C-terminal residues of the protein. Our results suggest that FtsK interacts directly with XerCD in order to activate recombination at dif... - Functional analysis of the C-terminal domains of the site-specific recombinases XerC and XerDHenrique Ferreira
Division of Molecular Genetics, Biochemistry Department, University of Oxford, UK
J Mol Biol 330:15-27. 2003.... - Independent positioning and action of Escherichia coli replisomes in live cellsRodrigo Reyes-Lamothe
Department of Biochemistry, University of Oxford, Oxford OX1 3 QU, UK
Cell 133:90-102. 2008..We conclude that independent replication forks follow the path of the compacted chromosomal DNA, with no structure other than DNA anchoring the replisome to any particular cellular region... - Separating speed and ability to displace roadblocks during DNA translocation by FtsKEstelle Crozat
Department of Biochemistry, University of Oxford, Oxford, UK
EMBO J 29:1423-33. 2010..This separation of translocation velocity and ability to displace roadblocks is more consistent with a sequential escort mechanism than stochastic, hand-off, or concerted mechanisms... - The two Escherichia coli chromosome arms locate to separate cell halvesXindan Wang
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
Genes Dev 20:1727-31. 2006.... - Stoichiometry and architecture of active DNA replication machinery in Escherichia coliRodrigo Reyes-Lamothe
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
Science 328:498-501. 2010..Single-strand binding protein has a broader spatial distribution than the core components, with 5 to 11 tetramers per replisome. This in vivo technique could provide single-molecule insight into other molecular machines... - Activation of XerCD-dif recombination by the FtsK DNA translocaseIan Grainge
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Nucleic Acids Res 39:5140-8. 2011.... - In vivo architecture and action of bacterial structural maintenance of chromosome proteinsAnjana Badrinarayanan
Department of Biochemistry, University of Oxford, UK
Science 338:528-31. 2012..Thus, by functioning in pairs, MukBEF complexes may undergo multiple cycles of ATP hydrolysis without being released from DNA, analogous to the behavior of well-characterized molecular motors... - mwr Xer site-specific recombination is hypersensitive to DNA supercoilingSonia Trigueros
Bionanotechnology IRC Department of Physics, University of Oxford, Oxford OX1 3QU, UK
Nucleic Acids Res 37:3580-7. 2009.... - Modulation of Escherichia coli sister chromosome cohesion by topoisomerase IVXindan Wang
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
Genes Dev 22:2426-33. 2008..Therefore, we propose that precatenanes, which form as replication progresses by interwinding of newly replicated sister chromosomes, are responsible for E. coli sister chromosome cohesion... - Accessory factors determine the order of strand exchange in Xer recombination at psiMigena Bregu
Microbiology Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
EMBO J 21:3888-97. 2002..This finding has important implications for the way in which accessory proteins interact with the recombinases... - Spatial and temporal organization of replicating Escherichia coli chromosomesIvy F Lau
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Mol Microbiol 49:731-43. 2003..This asymmetry could provide a mechanism by which the chromosome segregation protein FtsK, located at the division septum, can act directionally to ensure that the septal region is free of DNA before the completion of cell division... - Independent segregation of the two arms of the Escherichia coli ori region requires neither RNA synthesis nor MreB dynamicsXindan Wang
Department of Biochemistry, University of Oxford, South Parks Rd, Oxford, United Kingdom
J Bacteriol 192:6143-53. 2010..Inhibition of RNA synthesis and inhibition of the dynamic polymerization of the actin homolog MreB did not affect ori and bulk chromosome segregation... - Bacterial chromosome dynamicsDavid J Sherratt
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Science 301:780-5. 2003..Bacteria specify positional information, which determines where cell division will occur and which places the replication machinery and chromosomal loci at defined locations that change during cell cycle progression... - Visualizing genetic loci and molecular machines in living bacteriaXindan Wang
Department of Biochemistry, University of Oxford, South Parks Road, Oxford, UK
Biochem Soc Trans 36:749-53. 2008.... - A streptavidin variant with slower biotin dissociation and increased mechanostabilityClaire E Chivers
Department of Biochemistry, Oxford University, UK
Nat Methods 7:391-3. 2010..FtsK, a motor protein important in chromosome segregation, rapidly displaced streptavidin from biotinylated DNA, whereas traptavidin resisted displacement, indicating the force generated by Ftsk translocation... - Sequence-specific assembly of FtsK hexamers establishes directional translocation on DNAJames E Graham
Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, United Kingdom
Proc Natl Acad Sci U S A 107:20263-8. 2010..We also estimate the ATP coupling efficiency of translocation to be 1.63-2.11 bp of dsDNA translocated/ATP hydrolyzed. The data were used to derive a model for the assembly, initiation, and translocation of FtsK hexamers... - fpr, a deficient Xer recombination site from a Salmonella plasmid, fails to confer stability by dimer resolution: comparative studies with the pJHCMW1 mwr siteTung Tran
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
J Bacteriol 192:883-7. 2010..Mutagenesis and comparative studies with mwr, a site closely related to fpr, indicate that there is an interdependence of the sequences in the XerC binding region and the central region in Xer site-specific recombination sites... - Replication and segregation of an Escherichia coli chromosome with two replication originsXindan Wang
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
Proc Natl Acad Sci U S A 108:E243-50. 2011..In all strains, spatial separation of sister loci adjacent to active origins occurred shortly after their replication, independently of whether replication initiated at the normal origin, the ectopic origin, or both origins... - Engineering a 2D protein-DNA crystalJonathan Malo
Department of Physics, Clarendon Laboratory, University of Oxford, Parks Road, Oxford OX1 3PU, UK
Angew Chem Int Ed Engl 44:3057-61. 2005 - Osmoregulation of dimer resolution at the plasmid pJHCMW1 mwr locus by Escherichia coli XerCD recombinationHuong Pham
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
J Bacteriol 184:1607-16. 2002..coli ARG box consensus. The central region of the mwr core recombination site plays a role in regulation of site-specific recombination by the osmotic pressure of the medium...