G J Sharples

Summary

Affiliation: University of Nottingham
Country: UK

Publications

  1. ncbi request reprint The X philes: structure-specific endonucleases that resolve Holliday junctions
    G J Sharples
    Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK
    Mol Microbiol 39:823-34. 2001
  2. pmc DNA structure specificity of Rap endonuclease
    G J Sharples
    Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK
    Nucleic Acids Res 27:4121-7. 1999
  3. ncbi request reprint Genetic analysis of an archaeal Holliday junction resolvase in Escherichia coli
    E L Bolt
    Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, NG7 2UH, UK
    J Mol Biol 310:577-89. 2001
  4. pmc Resolution of Holliday intermediates in recombination and DNA repair: indirect suppression of ruvA, ruvB, and ruvC mutations
    T N Mandal
    Department of Genetics, University of Nottingham, Queens Medical Centre, United Kingdom
    J Bacteriol 175:4325-34. 1993
  5. pmc Dissociation of synthetic Holliday junctions by E. coli RecG protein
    R G Lloyd
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    EMBO J 12:17-22. 1993
  6. pmc Recombination-dependent growth in exonuclease-depleted recBC sbcBC strains of Escherichia coli K-12
    L Ryder
    Department of Genetics, University of Nottingham, Queens Medical Centre, United Kingdom
    Genetics 143:1101-14. 1996
  7. pmc An E. coli RuvC mutant defective in cleavage of synthetic Holliday junctions
    G J Sharples
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Nucleic Acids Res 21:3359-64. 1993
  8. pmc Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions
    G J Sharples
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    EMBO J 13:6133-42. 1994
  9. pmc Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes
    F E Benson
    Department of Genetics, Queen s Medical Centre, University of Nottingham, UK
    Nucleic Acids Res 16:1541-9. 1988
  10. pmc Processing of recombination intermediates by the RecG and RuvAB proteins of Escherichia coli
    R G Lloyd
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Nucleic Acids Res 21:1719-25. 1993

Collaborators

  • E L Bolt
  • M C Whitby
  • S C West
  • D W Rice
  • D R Leach
  • R G Lloyd
  • A A Mahdi
  • F Martin
  • L Ryder
  • J B Rafferty
  • B Martin
  • T N Mandal
  • H J Dunderdale
  • S Eiler
  • J Gangloff
  • D Moras
  • G Eriani
  • D Hargreaves
  • J P Claverys
  • P J Artymiuk
  • S E Sedelnikova
  • O Humbert
  • P J Baker
  • F E Benson
  • B Connolly
  • C A Parsons
  • G T Illing

Detail Information

Publications24

  1. ncbi request reprint The X philes: structure-specific endonucleases that resolve Holliday junctions
    G J Sharples
    Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK
    Mol Microbiol 39:823-34. 2001
    ..Recent studies have brought about a reappraisal of the origins of junction-specific endonucleases with the discovery that RuvC, CCE1 and A22R share a common catalytic core...
  2. pmc DNA structure specificity of Rap endonuclease
    G J Sharples
    Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK
    Nucleic Acids Res 27:4121-7. 1999
    ..Almost no binding or cleavage was detected with duplex, partial duplex and single-stranded DNA. Thus Rap endonuclease shows a bias for structures that resemble D-loop and Holliday junction recombination intermediates...
  3. ncbi request reprint Genetic analysis of an archaeal Holliday junction resolvase in Escherichia coli
    E L Bolt
    Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, NG7 2UH, UK
    J Mol Biol 310:577-89. 2001
    ..These results support models in which RecG acts at a replication fork stalled at a lesion in the DNA, catalysing fork regression and forming a Holliday junction that can then be acted upon by Hjc...
  4. pmc Resolution of Holliday intermediates in recombination and DNA repair: indirect suppression of ruvA, ruvB, and ruvC mutations
    T N Mandal
    Department of Genetics, University of Nottingham, Queens Medical Centre, United Kingdom
    J Bacteriol 175:4325-34. 1993
    ..The possibility that this additional factor is a junction-specific resolvase is discussed...
  5. pmc Dissociation of synthetic Holliday junctions by E. coli RecG protein
    R G Lloyd
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    EMBO J 12:17-22. 1993
    ..We suggest that RecG and RuvAB provide alternative activities than can promote branch migration of Holliday junctions in recombination and DNA repair...
  6. pmc Recombination-dependent growth in exonuclease-depleted recBC sbcBC strains of Escherichia coli K-12
    L Ryder
    Department of Genetics, University of Nottingham, Queens Medical Centre, United Kingdom
    Genetics 143:1101-14. 1996
    ..Recombination then becomes necessary to repair the fork and allow chromosome duplication to be completed. The possibility that RdgC is an exonuclease is discussed...
  7. pmc An E. coli RuvC mutant defective in cleavage of synthetic Holliday junctions
    G J Sharples
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Nucleic Acids Res 21:3359-64. 1993
    ..Neither of these genes produces a stable product. The results suggest that the N-terminal domain of RuvC may be concerned with cleavage of junctions...
  8. pmc Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions
    G J Sharples
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    EMBO J 13:6133-42. 1994
    ..We conclude that all ruv strains are defective in junction cleavage, and discuss pathways for Holliday junction resolution by RuvAB, RuvC, RecG and Rus...
  9. pmc Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes
    F E Benson
    Department of Genetics, Queen s Medical Centre, University of Nottingham, UK
    Nucleic Acids Res 16:1541-9. 1988
    ..The amino acid sequence predicted for RuvB contains a region that matches a highly conserved sequence found in several DNA repair and recombination proteins that bind ATP...
  10. pmc Processing of recombination intermediates by the RecG and RuvAB proteins of Escherichia coli
    R G Lloyd
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Nucleic Acids Res 21:1719-25. 1993
    ..However, RecG shows no ability to unwind more conventional substrates and the suggestion is made that its helicase activity is directed towards specific DNA structures such as junctions...
  11. pmc Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12
    R G Lloyd
    Department of Genetics, Queens Medical Centre, University of Nottingham, United Kingdom
    J Bacteriol 173:6837-43. 1991
    ..The primary sequence of RecG revealed that it is related to proteins that act as helicases and has a well-conserved motif identified with ATP binding...
  12. ncbi request reprint Molecular and functional analysis of the ruv region of Escherichia coli K-12 reveals three genes involved in DNA repair and recombination
    G J Sharples
    Genetics Department, University of Nottingham, Queens Medical Centre, UK
    Mol Gen Genet 221:219-26. 1990
    ..Two putative ruv deletion mutants were shown to be the result of deletion-inversion events mediated during imprecise excision of Tn10. The relevant inversion breakpoints in these mutants were located to ruvA and ruvC...
  13. pmc Location of the Bacillus subtilis sbcD gene downstream of addAB, the analogues of E. coli recBC
    G J Sharples
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Nucleic Acids Res 21:2010. 1993
  14. pmc Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein
    G J Sharples
    Department of Genetics, University of Nottingham, Queens Medical Centre, United Kingdom
    J Bacteriol 173:7711-5. 1991
    ..A double ribosomal frameshift between these genes is proposed as a possible reason for the low level of RuvC. Two further open reading frames of unknown function were identified, one on either side of the orf-33-ruvC operon...
  15. ncbi request reprint Location of a mutation in the aspartyl-tRNA synthetase gene of Escherichia coli K12
    G J Sharples
    Department of Genetics, University of Nottingham, Medical School, Queens Medical Centre, Great Britain
    Mutat Res 264:93-6. 1991
    ..3 kb from the 5' end of the ruvAB operon. A DNA fragment encoding the carboxy-terminus of AspRS was found to be sufficient to allow growth of a tls-1 strain at the non-permissive temperature...
  16. pmc A novel repeated DNA sequence located in the intergenic regions of bacterial chromosomes
    G J Sharples
    Department of Genetics, University of Nottingham, Medical School, Queens Medical Centre, UK
    Nucleic Acids Res 18:6503-8. 1990
    ..Some of the IRU sequences are transcribed, others are probably not. The structure and possible functions of these sequences are discussed in relation to palindromic units and other repeated DNA sequences in bacteria...
  17. ncbi request reprint Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins
    H J Dunderdale
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK
    Nature 354:506-10. 1991
    ..The newly identified RuvC protein resolves the intermediates by specific endonucleolytic cleavage to produce recombinant DNA molecules...
  18. pmc Resolution of Holliday junctions in vitro requires the Escherichia coli ruvC gene product
    B Connolly
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, United Kingdom
    Proc Natl Acad Sci U S A 88:6063-7. 1991
    ..The UV sensitivity and deficiency in recombinational repair of DNA exhibited by ruv mutants lead us to suggest that RuvC resolves Holliday junctions in vivo...
  19. ncbi request reprint Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction
    J B Rafferty
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Science 274:415-21. 1996
    ..The model presented reveals how a RuvAB-junction complex may also accommodate the resolvase RuvC...
  20. ncbi request reprint Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase
    H J Dunderdale
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, U K
    J Biol Chem 269:5187-94. 1994
    ..0). No energy cofactors are needed. When RuvC was analyzed by gel filtration and polyacrylamide gel electrophoresis, monomeric and dimeric forms of the protein were observed...
  21. ncbi request reprint Structural and functional similarities between the SbcCD proteins of Escherichia coli and the RAD50 and MRE11 (RAD32) recombination and repair proteins of yeast
    G J Sharples
    Mol Microbiol 17:1215-7. 1995
  22. ncbi request reprint Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82
    A A Mahdi
    Department of Genetics, University of Nottingham Queens Medical Centre, UK
    J Mol Biol 257:561-73. 1996
    ..Multicopy plasmids expressing RusA alone make ruvA, ruvB, and ruvC mutants resistant to UV light. Suppression depends critically on RecG...
  23. ncbi request reprint The mmsA locus of Streptococcus pneumoniae encodes a RecG-like protein involved in DNA repair and in three-strand recombination
    B Martin
    Microbiologie et Génétique Moléculaire CNRS UPR 9007, Universite Paul Sabatier, Toulouse, France
    Mol Microbiol 19:1035-45. 1996
    ..Therefore, the pms mutation, although it affected mismatch repair and, to some extent, DNA repair and recombination, acted as a suppressor of the mmsA mutation...
  24. pmc Characterization of a thermosensitive Escherichia coli aspartyl-tRNA synthetase mutant
    F Martin
    UPR 9002, Structure des Macromolecules Biologiques et Mecanismes de Reconnaissance, CNRS, Institut de Biologie Moleculaire et Cellulaire, Strasbourg, France
    J Bacteriol 179:3691-6. 1997
    ....