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Genomes and Genes | C M ShanahanSummaryAffiliation: University of Cambridge Country: UK Publications
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Publications
Aquaporin-1 is expressed by vascular smooth muscle cells and mediates rapid water transport across vascular cell membranesC M Shanahan
Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
J Vasc Res 36:353-62. 1999..By analogy with other tissues, these data argue for an important role for AQP-1 in regulating transcellular fluid flow and tissue hydration...- A potential role for sterol 27-hydroxylase in atherogenesisC M Shanahan
Department of Medicine, University of Cambridge, Box 157, Addenbrooke s Hospital, Hills Road, CB2 2QQ, Cambridge, UK
Atherosclerosis 154:269-76. 2001.... - Mechanisms of vascular calcification in renal diseaseC M Shanahan
Department of Medicine, University of Cambridge, Cambridge, UK
Clin Nephrol 63:146-57. 2005..We have synthesized these findings into a theoretical model offering a putative pathway for the development of severe vascular calcification in end-stage renal disease... - Expression of mineralisation-regulating proteins in association with human vascular calcificationC M Shanahan
University of Cambridge, Department of Medicine, ACCI, Addenbrooke s Hospital, UK
Z Kardiol 89:63-8. 2000.... - Smooth muscle cell phenotypes in atherosclerotic lesionsC M Shanahan
Department of Medicine, University of Cambridge, UK
Curr Opin Lipidol 10:507-13. 1999..Therefore an understanding of the processes that generate and regulate vascular smooth muscle cell heterogeneity are of critical importance for future therapeutic advancement in the treatment of atherosclerosis... - Medial localization of mineralization-regulating proteins in association with Mönckeberg's sclerosis: evidence for smooth muscle cell-mediated vascular calcificationC M Shanahan
Department of Medicine, Addenbrooke s Hospital, Cambridge, UK
Circulation 100:2168-76. 1999..Therefore, we examined gene expression in vessels with MS to determine whether there was evidence for a regulated calcification process in the vessel media... - Vascular calcificationCatherine M Shanahan
Department of Medicine, University of Cambridge, Cambridge, UK
Curr Opin Nephrol Hypertens 14:361-7. 2005..This review focuses on recent cell biological and animal studies that have shed light on the mechanisms and regulators of vascular smooth muscle cell calcification in end-stage renal disease... - Identification of osteoglycin as a component of the vascular matrix. Differential expression by vascular smooth muscle cells during neointima formation and in atherosclerotic plaquesC M Shanahan
University of Cambridge, Department of Medicine, Addenbrooke s Hospital, UK
Arterioscler Thromb Vasc Biol 17:2437-47. 1997..These data indicate that osteoglycin is a new marker of differentiated VSMCs and may be an essential component of the normal vascular matrix... - A polymorphism of the human matrix gamma-carboxyglutamic acid protein promoter alters binding of an activating protein-1 complex and is associated with altered transcription and serum levelsA Farzaneh-Far
Division of Cardiovascular Medicine, University of Cambridge, Addenbrooke s Hospital ACCI level 6, Hills Road, Cambridge CB2 2QQ, United Kingdom
J Biol Chem 276:32466-73. 2001..This could have important implications for diseases such as atherosclerosis and aortic valve stenosis, since it strongly suggests a genetic basis for regulation of tissue calcification... - Acetylated low-density lipoprotein stimulates human vascular smooth muscle cell calcification by promoting osteoblastic differentiation and inhibiting phagocytosisD Proudfoot
Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
Circulation 106:3044-50. 2002..We therefore tested the hypothesis that lipid components in atherosclerotic lesions influenced VSMC phenotype and calcification using an in vitro model of calcification... - Polyubiquitin is a new phenotypic marker of contractile vascular smooth muscle cellsP J Adam
Department of Medicine, Addenbrookes Hospital, University of Cambridge, UK
Cardiovasc Res 33:416-21. 1997..The aim of this study was to identify and characterise a 2.8 kb transcript, 2E10, which was highly expressed in freshly dispersed rat aortic VSMCs and downregulated in multiply passaged cultured VSMCs... - Isolation of gene markers of differentiated and proliferating vascular smooth muscle cellsC M Shanahan
Department of Biochemistry, University of Cambridge, UK
Circ Res 73:193-204. 1993..Therefore, they should aid in the analysis of gene expression during the development of vessel lesions... - Transcriptional regulation of matrix gla proteinA Farzaneh-Far
Division of Cardiovascular Medicine, University of Cambridge, Addenbrooke s Hospital ACCI level 6, Hills Road, Cambridge CB2 2QQ, UK
Z Kardiol 90:38-42. 2001..We found that MGP transcription is downregulated by retinoic acid and transforming growth factor beta (TGF beta) whereas it is upregulated by vitamin D3 and cyclic AMP... - The role of apoptosis in the initiation of vascular calcificationD Proudfoot
Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Level 6, ACCI Building, Hills Road, Cambridge CB2 2QQ, UK
Z Kardiol 90:43-6. 2001..These observations suggest that apoptosis is involved in the development of VSMC calcification and that VSMC-derived ABs have similarities with matrix vesicles... - Vascular smooth muscle cell phenotypic plasticity and the regulation of vascular calcificationV P Iyemere
Department of Medicine, Division of Cardiovascular Medicine, University of Cambridge, Cambridge, UK
J Intern Med 260:192-210. 2006..Understanding of the mechanisms and signalling pathways involved in this particular phenotype change is well advanced offering the possibility for the design of successful therapeutic interventions in the future... - Calcification of human vascular cells in vitro is correlated with high levels of matrix Gla protein and low levels of osteopontin expressionD Proudfoot
Department of Medicine, University of Cambridge, Addenbrooke s Hospital, England
Arterioscler Thromb Vasc Biol 18:379-88. 1998..These studies reveal that human VSMCs are capable of inducing calcification and that MGP may have a role in human vascular calcification... - Matrix gla protein is regulated by a mechanism functionally related to the calcium-sensing receptorA Farzaneh-Far
Division of Cardiovascular Medicine, University of Cambridge, Addenbrooke s Hospital, ACCI level 6, Hills Road, Cambridge, CB2 2QQ, United Kingdom
Biochem Biophys Res Commun 277:736-40. 2000..We therefore propose that this novel cation sensing mechanism may play a homeostatic role in preventing pathological calcification... 
High expression of genes for calcification-regulating proteins in human atherosclerotic plaquesC M Shanahan
Department of Medicine, Addenbrooke s Hospital, Cambridge, United Kingdom
J Clin Invest 93:2393-402. 1994..The postulated function of OP and MGP as regulators of calcification in bone and the high levels and colocalization of both in atheromatous plaques suggest they have an important role in plaque pathogenesis and stability...- Biology of calcification in vascular cells: intima versus mediaD Proudfoot
Division of Cardiovascular Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK
Herz 26:245-51. 2001..CLINICAL IMPORTANCE: Since both types of calcification correlate with significant morbidity and mortality, we discuss the different types of calcification in terms of their clinical importance... - Nesprins: a novel family of spectrin-repeat-containing proteins that localize to the nuclear membrane in multiple tissuesQ Zhang
Department of Medicine, Division of Cardiovascular Medicine, University of Cambridge, Box 110, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2QQ, UK
J Cell Sci 114:4485-98. 2001..These data and structural analogies with other proteins suggest that nesprins may function as 'dystrophins of the nucleus' to maintain nuclear organization and structural integrity... - Apoptosis regulates human vascular calcification in vitro: evidence for initiation of vascular calcification by apoptotic bodiesD Proudfoot
Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
Circ Res 87:1055-62. 2000..These observations provide evidence that apoptosis precedes VSMC calcification and that apoptotic bodies derived from VSMCs may act as nucleating structures for calcium crystal formation... - Osteo/chondrocytic transcription factors and their target genes exhibit distinct patterns of expression in human arterial calcificationKerry L Tyson
Department of Medicine, Addenbrooke s Hospital, Cambridge, UK
Arterioscler Thromb Vasc Biol 23:489-94. 2003..This study aimed to determine the temporal and spatial pattern of expression of key bone and cartilage proteins as atherosclerotic calcification progresses... 
Mineral surface in calcified plaque is like that of bone: further evidence for regulated mineralizationMelinda J Duer
Department of Chemistry, University of Cambridge, Cambridge, UK
Arterioscler Thromb Vasc Biol 28:2030-4. 2008..This study was undertaken to compare the poorly understood interface in mineralized plaque with that of bone, which is considerably better characterized...- Calcium phosphate crystals induce cell death in human vascular smooth muscle cells: a potential mechanism in atherosclerotic plaque destabilizationAlexandra E Ewence
Division of Cardiovascular Medicine, University of Cambridge, ACCI Building, Level 6, Box 110, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QQ, UK
Circ Res 103:e28-34. 2008.... - Molecular mechanisms mediating vascular calcification: role of matrix Gla proteinDiane Proudfoot
Division of Cardiovascular Medicine, University of Cambridge, Addenbrooke s Hospital ACCI, Level 6, Cambridge, UK
Nephrology (Carlton) 11:455-61. 2006..Therefore, strategies aimed at increasing its expression and activity may be beneficial in tipping the balance in favour of inhibition of calcification in CKD... - Adipocytic differentiation and liver x receptor pathways regulate the accumulation of triacylglycerols in human vascular smooth muscle cellsJohn D Davies
Department of Medicine, University of Cambridge, ACCI, Box 110, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2QQ, United Kingdom
J Biol Chem 280:3911-9. 2005..Moreover, the development of an adipogenic phenotype in VSMC is consistent with their known phenotypic plasticity and may contribute to their dysfunction in atherosclerotic plaques and, thus, impinge on plaque growth and stability... - Nesprins: intracellular scaffolds that maintain cell architecture and coordinate cell function?Derek T Warren
Department of Medicine, ACCI, Level 6, Box 110, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2QQ, UK
Expert Rev Mol Med 7:1-15. 2005..We speculate that nesprin mutations might contribute to a broad range of human disease syndromes, including laminopathies... - Human vascular smooth muscle cells undergo vesicle-mediated calcification in response to changes in extracellular calcium and phosphate concentrations: a potential mechanism for accelerated vascular calcification in ESRDJoanne L Reynolds
Department of Medicine, Addenbrooke's Hospital, Cambridge, United Kingdom
J Am Soc Nephrol 15:2857-67. 2004..These vesicles contain mineralization inhibitors derived from VSMC and serum, and perturbation of the production or function of these inhibitors would lead to accelerated vascular calcification... - Exploring the biology of vascular calcification in chronic kidney disease: what's circulating?M Schoppet
Division of Cardiovascular Medicine, Addenbrooke s Hospital, Cambridge, UK
Kidney Int 73:384-90. 2008..A greater understanding of the origins and roles of potential circulating inhibitors may result in novel strategies aimed at the prevention or reversal of the life-limiting calcifying vasculopathies seen in CKD patients... - The nesprins are giant actin-binding proteins, orthologous to Drosophila melanogaster muscle protein MSP-300Qiuping Zhang
Department of Medicine, Division of Cardiovascular Medicine, University of Cambridge, Addenbrooke s Hospital, Hills Road, Box 110, Cambridge, CB2 2QQ, UK
Genomics 80:473-81. 2002.... - Nesprin-2 is a multi-isomeric protein that binds lamin and emerin at the nuclear envelope and forms a subcellular network in skeletal muscleQiuping Zhang
Department of Medicine, ACCI, Box 110, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2QQ, UK
J Cell Sci 118:673-87. 2005..Moreover, its association at the NE with lamin and emerin, the genes mutated in Emery-Dreifuss muscular dystrophy, suggests a mechanism to explain how disruption of the NE leads to muscle dysfunction... - Nesprin-1 and -2 are involved in the pathogenesis of Emery Dreifuss muscular dystrophy and are critical for nuclear envelope integrityQiuping Zhang
Department of Medicine, University of Cambridge, Cambridge, UK
Hum Mol Genet 16:2816-33. 2007..Taken together, these data suggest that EDMD may be caused, in part, by uncoupling of the nucleoskeleton and cytoskeleton because of perturbed nesprin/emerin/lamin interactions... - Krüppel-like factor 4 (KLF4/GKLF) is a target of bone morphogenetic proteins and transforming growth factor beta 1 in the regulation of vascular smooth muscle cell phenotypeKathryn E King
University of Cambridge, Department of Medicine, Addenbrooke s Centre for Clinical Investigation Level 6, Box 110 Addenbrooke s Hospital, Hills Rd, Cambridge CB2 2QQ, United Kingdom
J Biol Chem 278:11661-9. 2003..Taken together, our results demonstrate that multiple members of the TGF-beta superfamily act in concert to modulate VSMC phenotype... - Stromal cells of fibrodysplasia ossificans progressiva lesions express smooth muscle lineage markers and the osteogenic transcription factor Runx2/Cbfa-1: clues to a vascular origin of heterotopic ossification?Laszlo Hegyi
Division of Cardiovascular Medicine, Department of Medicine, University of Cambridge, Cambridge, UK
J Pathol 201:141-8. 2003.... - Multifunctional roles for serum protein fetuin-a in inhibition of human vascular smooth muscle cell calcificationJoanne L Reynolds
Division of Cardiovascular Medicine, Level 6, ACCI, Box 110, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, UK
J Am Soc Nephrol 16:2920-30. 2005..Strategies aimed at maintaining normal circulating levels of fetuin-A may prove beneficial in patients with ESRD... - Vascular calcification--a matter of damage limitation?Catherine M Shanahan
Nephrol Dial Transplant 21:1166-9. 2006 - Heterogeneity of gene expression in human atheroma unmasked using cDNA representational difference analysisKerry L Tyson
Department of Medicine, Division of Cardiovascular Medicine, Addenbrooke's Centre for Clinical Investigation, Addenbrooke's Hospital, Cambridge CB2 2QQ, United Kingdom
Physiol Genomics 9:121-30. 2002..In conclusion, cDNA RDA is a useful, fast, and efficient technique for studying differential gene expression particularly when clinical material is limiting... - Dialysis accelerates medial vascular calcification in part by triggering smooth muscle cell apoptosisRukshana C Shroff
Nephrology Unit, Great Ormond Street Hospital, University College London Institute of Child Health, King s College London, London SE5 9NU
Circulation 118:1748-57. 2008..To address this, we quantified the calcium (Ca) load in arteries from children (10 predialysis, 24 dialysis) and correlated it with clinical, biochemical, and vascular measures... - Distinct functional domains in nesprin-1alpha and nesprin-2beta bind directly to emerin and both interactions are disrupted in X-linked Emery-Dreifuss muscular dystrophyMatthew A Wheeler
The Randall Division of Cell and Molecular Biophysics, King s College, New Hunts House, Guy s Campus, London, UK
Exp Cell Res 313:2845-57. 2007.... - The vascular biology of calcificationRukshana C Shroff
Paediatric Nephrology Unit, Great Ormond Street Hospital for Children NHS Trust, London, UK
Semin Dial 20:103-9. 2007..A better understanding of the complex mechanisms regulating tissue calcification may have therapeutic potential in reducing the cardiovascular disease-associated morbidity and mortality in patients with renal disease... - Biology of vascular calcification in renal diseaseAfshin Farzaneh-Far
Division of Cardiology, New York Presbyterian Hospital, Cornell University Medical Center, New York, NY, USA
Nephron Exp Nephrol 101:e134-8. 2005..Better understanding of these mechanisms may have therapeutic potential in reducing the adverse cardiovascular event rates in patients with (ESRD)... - SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeletonFarhana Haque
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
Mol Cell Biol 26:3738-51. 2006..Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position... - Induction, differentiation, and remodeling of blood vessels after transplantation of Bcl-2-transduced endothelial cellsDavid R Enis
Interdepartmental Program in Vascular Biology and Transplantation, Boyer Center for Molecular Medicine, and Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA
Proc Natl Acad Sci U S A 102:425-30. 2005..Our results support the utility of differentiated EC transplantation to treat tissue ischemia... - Inflammation ushers in calcification: a cycle of damage and protection?Catherine M Shanahan
Circulation 116:2782-5. 2007 - Cell nuclei spin in the absence of lamin b1Julie Y Ji
Cardiovascular Division, Brigham and Women s Hospital, Cambridge, Massachusetts 02139, USA
J Biol Chem 282:20015-26. 2007..These findings demonstrate that lamin B1 serves a fundamental role within the nuclear envelope: anchoring the nucleus to the cytoskeleton...