LEONARD WILLIAM SEYMOUR

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. ncbi request reprint The future of gene therapy in the UK
    Leonard W Seymour
    Department of Clinical Pharmacology, University of Oxford, Radcliffe Infirmary, Woodstock Road, OX2 6HE, UK
    Trends Biotechnol 24:347-9. 2006
  2. pmc Targeting adenovirus gene delivery to activated tumour-associated vasculature via endothelial selectins
    Houria Bachtarzi
    Department of Clinical Pharmacology, University of Oxford, Old Road Campus, Headington, Oxford OX3 7DQ, UK
    J Control Release 150:196-203. 2011
  3. pmc Toward more effective gene delivery
    Hannah H Chen
    Oxford Institute for Cancer Medicine, University of Oxford, Old Road Campus, Oxford, OX3 7BN, UK
    Genome Biol 9:301. 2008
  4. ncbi request reprint Phase II studies of polymer-doxorubicin (PK1, FCE28068) in the treatment of breast, lung and colorectal cancer
    Leonard W Seymour
    Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham, UK
    Int J Oncol 34:1629-36. 2009
  5. ncbi request reprint Passive tumour targeting of polymer-coated adenovirus for cancer gene therapy
    Kerry D Fisher
    Department of Clinical Pharmacology, University of Oxford, Radcliffe Infirmary, Oxford, UK
    J Drug Target 15:546-51. 2007
  6. ncbi request reprint Vectors based on reducible polycations facilitate intracellular release of nucleic acids
    Martin L Read
    Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, UK
    J Gene Med 5:232-45. 2003
  7. doi request reprint HPMA copolymers for masking and retargeting of therapeutic viruses
    Kerry D Fisher
    University of Oxford, Department of Clinical Pharmacology, Old Road Campus Research Building, Old Road Campus, off Roosevelt Drive, Headington, Oxford OX3 7DQ United Kingdom
    Adv Drug Deliv Rev 62:240-5. 2010
  8. pmc Use of tissue-specific microRNA to control pathology of wild-type adenovirus without attenuation of its ability to kill cancer cells
    Ryan Cawood
    Department of Clinical Pharmacology, University of Oxford, Oxford, United Kingdom
    PLoS Pathog 5:e1000440. 2009
  9. doi request reprint Cetuximab retargeting of adenovirus via the epidermal growth factor receptor for treatment of intraperitoneal ovarian cancer
    Joanne Morrison
    Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, Oxford, United Kingdom
    Hum Gene Ther 20:239-51. 2009
  10. pmc Human erythrocytes bind and inactivate type 5 adenovirus by presenting Coxsackie virus-adenovirus receptor and complement receptor 1
    Robert C Carlisle
    Department of Clinical Pharmacology, University of Oxford, Oxford, United Kingdom
    Blood 113:1909-18. 2009

Research Grants

Collaborators

Detail Information

Publications30

  1. ncbi request reprint The future of gene therapy in the UK
    Leonard W Seymour
    Department of Clinical Pharmacology, University of Oxford, Radcliffe Infirmary, Woodstock Road, OX2 6HE, UK
    Trends Biotechnol 24:347-9. 2006
    ..In this personal appraisal, Professor Len Seymour assesses the progress of gene therapy in the UK and what it might deliver in the foreseeable future...
  2. pmc Targeting adenovirus gene delivery to activated tumour-associated vasculature via endothelial selectins
    Houria Bachtarzi
    Department of Clinical Pharmacology, University of Oxford, Old Road Campus, Headington, Oxford OX3 7DQ, UK
    J Control Release 150:196-203. 2011
    ..Employment of optimal viral modification using protein G will enable exploration and comparison of alternative targeting ligands targeting tumour-associated endothelium...
  3. pmc Toward more effective gene delivery
    Hannah H Chen
    Oxford Institute for Cancer Medicine, University of Oxford, Old Road Campus, Oxford, OX3 7BN, UK
    Genome Biol 9:301. 2008
    ..A report on the symposium 'In vivo barriers to gene delivery', Cold Spring Harbor, USA, 26-29 November 2007...
  4. ncbi request reprint Phase II studies of polymer-doxorubicin (PK1, FCE28068) in the treatment of breast, lung and colorectal cancer
    Leonard W Seymour
    Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham, UK
    Int J Oncol 34:1629-36. 2009
    ....
  5. ncbi request reprint Passive tumour targeting of polymer-coated adenovirus for cancer gene therapy
    Kerry D Fisher
    Department of Clinical Pharmacology, University of Oxford, Radcliffe Infirmary, Oxford, UK
    J Drug Target 15:546-51. 2007
    ..This technology provides an interesting option for delivery of therapeutic viruses to disseminated tumour masses by intravenous injection...
  6. ncbi request reprint Vectors based on reducible polycations facilitate intracellular release of nucleic acids
    Martin L Read
    Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, UK
    J Gene Med 5:232-45. 2003
    ..This article reports on the characterisation of a new type of synthetic vector based on a linear reducible polycation (RPC) that can be cleaved by the intracellular environment to facilitate release of nucleic acids...
  7. doi request reprint HPMA copolymers for masking and retargeting of therapeutic viruses
    Kerry D Fisher
    University of Oxford, Department of Clinical Pharmacology, Old Road Campus Research Building, Old Road Campus, off Roosevelt Drive, Headington, Oxford OX3 7DQ United Kingdom
    Adv Drug Deliv Rev 62:240-5. 2010
    ....
  8. pmc Use of tissue-specific microRNA to control pathology of wild-type adenovirus without attenuation of its ability to kill cancer cells
    Ryan Cawood
    Department of Clinical Pharmacology, University of Oxford, Oxford, United Kingdom
    PLoS Pathog 5:e1000440. 2009
    ..In addition to providing many potent new viruses for cancer virotherapy, microRNA control of virus replication should provide a new strategy for designing safe attenuated vaccines applied across a broad range of viral diseases...
  9. doi request reprint Cetuximab retargeting of adenovirus via the epidermal growth factor receptor for treatment of intraperitoneal ovarian cancer
    Joanne Morrison
    Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, Oxford, United Kingdom
    Hum Gene Ther 20:239-51. 2009
    ....
  10. pmc Human erythrocytes bind and inactivate type 5 adenovirus by presenting Coxsackie virus-adenovirus receptor and complement receptor 1
    Robert C Carlisle
    Department of Clinical Pharmacology, University of Oxford, Oxford, United Kingdom
    Blood 113:1909-18. 2009
    ..quot;Stealthing" of Ad5 using hydrophilic polymers may enable circumvention of these natural virus traps...
  11. doi request reprint Delivery of siRNA mediated by histidine-containing reducible polycations
    Mark Stevenson
    Department of Clinical Pharmacology, University of Oxford, Old Road Campus, Oxford OX3 7DQ, UK
    J Control Release 130:46-56. 2008
    ..siRNA targeted to the hepatitis B virus surface antigen delivered by CS-His6 RPC, mediated falls in both mRNA and protein expression, suggesting that this delivery system could be developed for potential therapies for viral hepatitis...
  12. doi request reprint Coating of adeno-associated virus with reactive polymers can ablate virus tropism, enable retargeting and provide resistance to neutralising antisera
    Robert C Carlisle
    Department of Clinical Pharmacology, Old Road Campus Research Building, University of Oxford, off Roosevelt Drive, Headington, Oxford, OX3 7DQ, UK
    J Gene Med 10:400-11. 2008
    ..Here we demonstrate polymer-coating techniques can also be used to modify and retarget adeno-associated virus (AAV) types 5 and 8...
  13. ncbi request reprint Virotherapy of ovarian cancer with polymer-cloaked adenovirus retargeted to the epidermal growth factor receptor
    Joanne Morrison
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Headington, Oxford, Oxfordshire, UK
    Mol Ther 16:244-51. 2008
    ....
  14. ncbi request reprint Emerging developments in RNA therapeutics
    Martin L Read
    University of Oxford, Department of Clinical Pharmacology, Radcliffe Infirmary, Woodstock Road, Oxford, OX2 6HE, UK
    Expert Opin Ther Targets 7:299-303. 2003
    ..Updates were also presented on the clinical and preclinical development of ribozymes and aptamers, including good progress in increasing the half-life of these molecules in serum...
  15. ncbi request reprint Adenoviral vectors: systemic delivery and tumor targeting
    Nicola K Green
    Department of Clinical Pharmacology, University of Oxford, Radcliffe Infirmary, Oxford, UK
    Cancer Gene Ther 9:1036-42. 2002
    ..In this review, we have summarized the various strategies used to overcome the limitations of adenoviral-mediated gene delivery...
  16. ncbi request reprint Polymer-coated polyethylenimine/DNA complexes designed for triggered activation by intracellular reduction
    Robert C Carlisle
    Department of Clinical Pharmacology, Oxford University, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, UK
    J Gene Med 6:337-44. 2004
    ..The use of biological trigger mechanisms provides one promising means to achieve this, and here we report a transfection trigger mechanism based on intracellular reduction...
  17. ncbi request reprint Hepatic drug targeting: phase I evaluation of polymer-bound doxorubicin
    Leonard W Seymour
    Cancer Research UK Institute for Cancer Studies, University of Birmingham, United Kingdom
    J Clin Oncol 20:1668-76. 2002
    ..The present phase I study was devised to determine the toxicity, pharmacokinetic profile, and targeting capability of PK2...
  18. doi request reprint Quantitative characterization of cell transduction by HSV-1 amplicons using flow cytometry and real-time PCR
    Yasser M El-Sherbini
    Department of Clinical Pharmacology, Old Road Campus Research Building, University of Oxford, Oxford OX3 7DQ, UK
    J Virol Methods 159:160-6. 2009
    ..3:1. Measuring the genomic copy/TU ratios is an important parameter for comparing the quality of amplicon preparations and standardizing experimental conditions...
  19. ncbi request reprint Importance of lateral and steric stabilization of polyelectrolyte gene delivery vectors for extended systemic circulation
    David Oupicky
    Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham, B15 2TT, UK
    Mol Ther 5:463-72. 2002
    ..Multivalent PHPMA coating allows, for the first time, considerably extended circulation time using polyplex systems-a prerequisite for systemic gene delivery...
  20. ncbi request reprint Factors influencing the ability of nuclear localization sequence peptides to enhance nonviral gene delivery
    K Helen Bremner
    Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham, B15 2TH, UK
    Bioconjug Chem 15:152-61. 2004
    ..Altogether, the results from this study should help to define the requirements for successful NLS-enhanced transfection...
  21. doi request reprint In vitro evaluation of a 'stealth' adenoviral vector for targeted gene delivery to adult mammalian neurones
    Karen Sims
    Molecular Neuroscience Group, School of Clinical and Experimental Medicine, University of Birmingham, Birmingham, UK
    J Gene Med 11:335-44. 2009
    ..Polymer coating of adenovirus type 5 (Ad5) particles produces a 'stealth' Ad5 (sAd5) that confers protection from immune recognition, blocks receptor-mediated uptake, and favours uptake into pinocytic cells...
  22. pmc Quantification of siRNA using competitive qPCR
    Wei Li Liu
    Department of Clinical Pharmacology, University of Oxford, Headington, Oxford, UK
    Nucleic Acids Res 37:e4. 2009
    ..We anticipate that cqPCR will allow accurate determination of siRNA pharmacokinetics following in vivo delivery, greatly facilitating development of therapeutic siRNA delivery strategies...
  23. ncbi request reprint Glycoviruses: chemical glycosylation retargets adenoviral gene transfer
    Oliver M T Pearce
    Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, UK
    Angew Chem Int Ed Engl 44:1057-61. 2005
  24. ncbi request reprint Identification of FGF receptor-binding peptides for cancer gene therapy
    Fukuto Maruta
    Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, UK
    Cancer Gene Ther 9:543-52. 2002
    ..These peptides should provide useful ligands for specific delivery of gene therapy vectors to clinically relevant targets...
  25. ncbi request reprint Adenovirus type 5 interactions with human blood cells may compromise systemic delivery
    Mark Lyons
    Department of Clinical Pharmacology, University of Oxford, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, UK
    Mol Ther 14:118-28. 2006
    ..This may increase antigen presentation following intravenous injection, while blood cell binding may both decrease access of the virus to extravascular targets and inhibit infection of cells to which the virus does gain access...
  26. doi request reprint Combination dual responsive polypeptide vectors for enhanced gene delivery
    Rujikan Nasanit
    School of Chemistry, University of Birmingham, UK
    Mol Biosyst 4:741-5. 2008
    ....
  27. ncbi request reprint Use of a phage display library to identify oligopeptides binding to the lumenal surface of polarized endothelium by ex vivo perfusion of human umbilical veins
    Fukuto Maruta
    Cancer Research UK Institute for Cancer Studies, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    J Drug Target 11:53-9. 2003
    ..These initial peptides may prove useful targeting agents for endothelial-selective delivery, and this powerful approach should be readily applicable to biopanning in a broad range of human vessels ex vivo...
  28. ncbi request reprint Development of long-circulating polyelectrolyte complexes for systemic delivery of genes
    David Oupicky
    CRC Institute for Cancer Studies, University of Birmingham, UK
    J Drug Target 10:93-8. 2002
    ..The most promising way to achieve this is by reductive activation of the vector within the cytoplasm or nucleus of the target cell. State of the art in this rapidly progressing field is reviewed...
  29. pmc Cytoplasmic expression systems triggered by mRNA yield increased gene expression in post-mitotic neurons
    Paul J Farrow
    Department of Clinical Pharmacology, University of Oxford, Oxford OX2 6HE, UK
    Nucleic Acids Res 34:e80. 2006
    ....
  30. ncbi request reprint Bacteriophage biopanning in human tumour biopsies to identify cancer-specific targeting ligands
    Fukuto Maruta
    Cancer Research UK Institute for Cancer Studies, University of Birmingham, and Department of Surgery, Selly Oak Hospital, UK
    J Drug Target 15:311-9. 2007
    ..This novel approach may be applicable to a wide range of settings, thus enabling iteration of consensus targeting sequences for tumour imaging and selective delivery of anticancer agents...

Research Grants2

  1. Starburst Viruses: A paradigm for modification of biological surfaces
    Leonard Seymour; Fiscal Year: 2007
    ..g. hepatitis) and inherited diseases (e.g. cystic fibrosis) and notably in development of antibody-resistant viral vaccines suitable for repeated use in diseases such as HIV, malaria, TB and influenza. ..