Genomes and Genes
Yotis A Senis
Affiliation: University of Birmingham
- Role of the p110delta PI 3-kinase in integrin and ITAM receptor signalling in plateletsYotis A Senis
Centre for Cardiovascular Sciences, Division of Medical Sciences, Institute of Biomedical Research, Wolfson Drive, The Medical School, University of Birmingham, Edgbaston, Birmingham, UK
Platelets 16:191-202. 2005..The more pronounced inhibitory effect of LY294002 and wortmannin indicates that other isoforms of PI 3-kinase play a more significant role in signalling by the two platelet glycoprotein receptors...
- Platelet proteomics: state of the art and future perspectiveYotis Senis
Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, University of Birmingham, Birmingham, UK
Methods Mol Biol 788:367-99. 2012..We also discuss new frontiers and applications of proteomics to platelets and microparticles in health and disease, as we strive to better understand the molecular mechanisms underlying the platelet response to vascular injury...
- A comprehensive proteomics and genomics analysis reveals novel transmembrane proteins in human platelets and mouse megakaryocytes including G6b-B, a novel immunoreceptor tyrosine-based inhibitory motif proteinYotis A Senis
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Wolfson Drive, Edgbaston, Birmingham B15 2TT, United Kingdom
Mol Cell Proteomics 6:548-64. 2007..G6b-B undergoes tyrosine phosphorylation and association with the SH2 domain-containing phosphatase, SHP-1, in stimulated platelets suggesting that it may play a novel role in limiting platelet activation...
- The tyrosine phosphatase CD148 is an essential positive regulator of platelet activation and thrombosisYotis A Senis
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, United Kingdom
Blood 113:4942-54. 2009..G protein-coupled receptor responses to thrombin and other agonists were also marginally reduced. These results highlight CD148 as a global regulator of platelet activation and a novel antithrombotic drug target...
- Isolation and characterization of cotiaractivase, a novel low molecular weight prothrombin activator from the venom of Bothrops cotiaraYotis A Senis
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, Wolfson Drive, Edgbaston, Birmingham B15 2TT, UK
Biochim Biophys Acta 1764:863-71. 2006..Cotiaractivase is a unique low molecular weight snake venom prothrombin activator that likely belongs to the metalloproteinase family of proteins...
- G6b-B inhibits constitutive and agonist-induced signaling by glycoprotein VI and CLEC-2Jun Mori
Centre for Cardiovascular Sciences, Institute of Biomedical Research, University of Birmingham, B15 2TT, UK
J Biol Chem 283:35419-27. 2008..We speculate that inhibition of constitutive signaling through Src and Syk tyrosine kinases by G6b-B may help to prevent unwanted platelet activation...
- Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1BJun Mori
Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
Arterioscler Thromb Vasc Biol 32:2956-65. 2012..In the present study, we compared the relative contributions of these PTPs in platelet activation by the major glycoprotein, glycoprotein VI, α(IIb)β(3), and C-type lectin-like receptor 2 (CLEC-2)...
- Minimal regulation of platelet activity by PECAM-1Tarvinder S Dhanjal
Centre for Cardiovascular Sciences, Institute of Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
Platelets 18:56-67. 2007....
- Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signalingSonia Severin
Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
J Biol Chem 286:4107-16. 2011....
- Mice lacking the ITIM-containing receptor G6b-B exhibit macrothrombocytopenia and aberrant platelet functionAlexandra Mazharian
Centre of Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK
Sci Signal 5:ra78. 2012..Together, these findings establish G6b-B as a major inhibitory receptor regulating megakaryocyte activation, function, and platelet production...
- Adhesion of human and mouse platelets to collagen under shear: a unifying modelJocelyn M Auger
Centre for Cardiovascular Sciences, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham, UK
FASEB J 19:825-7. 2005..These observations form the basis of a unifying two-state model of platelet adhesion and aggregate formation on collagen that is conserved between human and mouse platelets...
- Identification of Tspan9 as a novel platelet tetraspanin and the collagen receptor GPVI as a component of tetraspanin microdomainsMajd B Protty
Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham B152TT, UK
Biochem J 417:391-400. 2009..These findings suggest a role for Tspan9 in regulating platelet function in concert with other platelet tetraspanins and their associated proteins...
- Vav1 and vav3 have critical but redundant roles in mediating platelet activation by collagenAndrew C Pearce
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK
J Biol Chem 279:53955-62. 2004..This is the first time that absolute redundancy between two protein isoforms has been observed with respect to the regulation of PLC gamma 2 in platelets...
- A global proteomics approach identifies novel phosphorylated signaling proteins in GPVI-activated platelets: involvement of G6f, a novel platelet Grb2-binding membrane adapterAngel Garcia
Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford, UK
Proteomics 6:5332-43. 2006..This study provides new insights into the mechanism of platelet activation through the GPVI collagen receptor, helping to build the basis for the development of new drug targets for thrombotic disease...
- Characterization of a novel protein from Proatheris superciliaris venom: proatherocytin, a 34-kDa platelet receptor PAR1 agonistGavin D Laing
Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
Toxicon 46:490-9. 2005..These results suggest that proatherocytin is a highly selective PAR1 agonist that also causes mouse platelet aggregation, probably through cleavage of PAR4...
- GPIb-dependent platelet activation is dependent on Src kinases but not MAP kinase or cGMP-dependent kinaseStuart J Marshall
Department of Pharmacology, University of Oxford, UK
Blood 103:2601-9. 2004..These results demonstrate a critical role for Src kinases but not MAP kinases in VWF-dependent platelet activation and demonstrate an inhibitory role for cGMP-elevating agents in regulating this process...
- Fps/Fes and Fer protein-tyrosinekinases play redundant roles in regulating hematopoiesisYotis A Senis
Department of Pathology, Queen s University, Kingston, Ontario, Canada
Exp Hematol 31:673-81. 2003..In this study, knock-in mice harboring kinase-inactivating mutations in both fps and fer alleles were used to assess functional redundancy between Fps and Fer kinases in regulating hematopoiesis...
- GPVI levels in platelets: relationship to platelet function at high shearDenise Best
Department of Pharmacology, Oxford University, Mansfield Rd, Oxford, OX1 3QT, United Kingdom
Blood 102:2811-8. 2003..These results demonstrate that GPVI levels are tightly controlled and play a critical role in thrombus formation on collagen; nevertheless, a range of receptor densities can support platelet function under high shear...
- Absence of Fer protein-tyrosine kinase exacerbates leukocyte recruitment in response to endotoxinDonna Marie McCafferty
Immunology Research Group, Department of Medical Physiology, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada
J Immunol 168:4930-5. 2002..These data provide the first evidence for a biological role for Fer in regulation of leukocyte recruitment during the innate immune response...