Sjors H W Scheres

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. pmc A Bayesian view on cryo-EM structure determination
    Sjors H W Scheres
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    J Mol Biol 415:406-18. 2012
  2. ncbi request reprint Beam-induced motion correction for sub-megadalton cryo-EM particles
    Sjors Hw Scheres
    Structural Studies, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    elife 3:e03665. 2014
  3. pmc Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles
    Xiao Chen Bai
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    elife 2:e00461. 2013
  4. pmc RELION: implementation of a Bayesian approach to cryo-EM structure determination
    Sjors H W Scheres
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    J Struct Biol 180:519-30. 2012
  5. pmc Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome
    Israel S Fernández
    MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK
    Cell 157:823-31. 2014
  6. pmc Structure of the yeast mitochondrial large ribosomal subunit
    Alexey Amunts
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Science 343:1485-9. 2014
  7. pmc Molecular architecture of a eukaryotic translational initiation complex
    Israel S Fernández
    MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK
    Science 342:1240585. 2013
  8. pmc Cryo-EM structure of a 3D DNA-origami object
    Xiao Chen Bai
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom
    Proc Natl Acad Sci U S A 109:20012-7. 2012
  9. pmc High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy
    Shaoxia Chen
    MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, U K
    Ultramicroscopy 135:24-35. 2013
  10. pmc Structure of active dimeric human telomerase
    Anselm Sauerwald
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    Nat Struct Mol Biol 20:454-60. 2013

Collaborators

Detail Information

Publications11

  1. pmc A Bayesian view on cryo-EM structure determination
    Sjors H W Scheres
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    J Mol Biol 415:406-18. 2012
    ..Application to experimental data indicates that the statistical approach yields more reliable structures than existing methods and is capable of detecting smaller classes in data sets that contain multiple different structures...
  2. ncbi request reprint Beam-induced motion correction for sub-megadalton cryo-EM particles
    Sjors Hw Scheres
    Structural Studies, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    elife 3:e03665. 2014
    ..Application of the new algorithm to four data sets illustrates its potential for significantly improving cryo-EM structures, even for particles that are smaller than 200 kDa.DOI: http://dx.doi.org/10.7554/eLife.03665.001. ..
  3. pmc Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles
    Xiao Chen Bai
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    elife 2:e00461. 2013
    ..Therefore, this methodology may expand the scope of high-resolution cryo-EM to a broad range of biological specimens.DOI:http://dx.doi.org/10.7554/eLife.00461.001...
  4. pmc RELION: implementation of a Bayesian approach to cryo-EM structure determination
    Sjors H W Scheres
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    J Struct Biol 180:519-30. 2012
    ..The resulting implementation yields high-quality reconstructions and reliable resolution estimates with minimal user intervention and at acceptable computational costs...
  5. pmc Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome
    Israel S Fernández
    MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK
    Cell 157:823-31. 2014
    ..Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation...
  6. pmc Structure of the yeast mitochondrial large ribosomal subunit
    Alexey Amunts
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Science 343:1485-9. 2014
    ..The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site. ..
  7. pmc Molecular architecture of a eukaryotic translational initiation complex
    Israel S Fernández
    MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK
    Science 342:1240585. 2013
    ..The relatively high resolution obtained from such a small fraction of a heterogeneous sample suggests a general approach for characterizing the structure of other dynamic or transient biological complexes...
  8. pmc Cryo-EM structure of a 3D DNA-origami object
    Xiao Chen Bai
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom
    Proc Natl Acad Sci U S A 109:20012-7. 2012
    ..Thereby, our results indicate an attractive route to fabricate nanoscale devices that achieve complex functionalities by DNA-templated design steered by structural feedback...
  9. pmc High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy
    Shaoxia Chen
    MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, U K
    Ultramicroscopy 135:24-35. 2013
    ..Applying this procedure to cryoEM images of beta-galactosidase shows how overfitting varies greatly depending on the procedure, but in the best case shows no overfitting and a resolution of ~6 Å. (382 words)...
  10. pmc Structure of active dimeric human telomerase
    Anselm Sauerwald
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    Nat Struct Mol Biol 20:454-60. 2013
    ....
  11. pmc Structure of the mammalian ribosome-Sec61 complex to 3.4 Å resolution
    Rebecca M Voorhees
    MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK Electronic address
    Cell 157:1632-43. 2014
    ..The high-resolution structure of the mammalian ribosome-Sec61 complex provides a valuable reference for future functional and structural studies. ..