Leonid A Sazanov

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi request reprint Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus
    Leonid A Sazanov
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, U K
    Science 311:1430-6. 2006
  2. doi request reprint The architecture of respiratory complex I
    Rouslan G Efremov
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Nature 465:441-5. 2010
  3. ncbi request reprint A role for native lipids in the stabilization and two-dimensional crystallization of the Escherichia coli NADH-ubiquinone oxidoreductase (complex I)
    Leonid A Sazanov
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:19483-91. 2003
  4. ncbi request reprint Organization of iron-sulfur clusters in respiratory complex I
    Philip Hinchliffe
    Medical Research Council MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Science 309:771-4. 2005
  5. doi request reprint Three-dimensional structure of respiratory complex I from Escherichia coli in ice in the presence of nucleotides
    David J Morgan
    Medical Research Council, Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochim Biophys Acta 1777:711-8. 2008
  6. ncbi request reprint Projection structure of the membrane domain of Escherichia coli respiratory complex I at 8 A resolution
    Ekaterina A Baranova
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    J Mol Biol 366:140-54. 2007
  7. ncbi request reprint Substrate-induced conformational change in bacterial complex I
    Aygun A Mamedova
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:23830-6. 2004
  8. ncbi request reprint Respiratory complex I: mechanistic and structural insights provided by the crystal structure of the hydrophilic domain
    Leonid A Sazanov
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK sazanov mrc dunn cam ac uk
    Biochemistry 46:2275-88. 2007
  9. ncbi request reprint Identification of a novel subunit of respiratory complex I from Thermus thermophilus
    Philip Hinchliffe
    Medical Research Council, Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochemistry 45:4413-20. 2006
  10. pmc Crystal structure of the entire respiratory complex I
    Rozbeh Baradaran
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Nature 494:443-8. 2013

Collaborators

  • Rouslan G Efremov
  • Ekaterina A Baranova
  • David J Morgan
  • Rozbeh Baradaran
  • Philip Hinchliffe
  • John M Berrisford
  • Peter J Holt
  • Chui Ying Yip
  • Joe Carroll
  • Aygun A Mamedova
  • Gurdeep S Minhas
  • Ian M Fearnley
  • Kamburapola Jayawardena
  • Michael E Harbour
  • Christopher J Thompson

Detail Information

Publications17

  1. ncbi request reprint Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus
    Leonid A Sazanov
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, U K
    Science 311:1430-6. 2006
    ..The novel subunit Nqo15 has a similar fold to the mitochondrial iron chaperone frataxin, and it may be involved in iron-sulfur cluster regeneration in the complex...
  2. doi request reprint The architecture of respiratory complex I
    Rouslan G Efremov
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Nature 465:441-5. 2010
    ....
  3. ncbi request reprint A role for native lipids in the stabilization and two-dimensional crystallization of the Escherichia coli NADH-ubiquinone oxidoreductase (complex I)
    Leonid A Sazanov
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:19483-91. 2003
    ..Our results also indicate that native lipids play an important role in the activation, stabilization and, as a consequence, crystallization of purified complex I from E. coli...
  4. ncbi request reprint Organization of iron-sulfur clusters in respiratory complex I
    Philip Hinchliffe
    Medical Research Council MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Science 309:771-4. 2005
    ..Cluster N1a may play a role in the prevention of oxidative damage. The structure provides a framework for the interpretation of the large amounts of data accumulated on complex I...
  5. doi request reprint Three-dimensional structure of respiratory complex I from Escherichia coli in ice in the presence of nucleotides
    David J Morgan
    Medical Research Council, Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochim Biophys Acta 1777:711-8. 2008
    ..The model of the entire bacterial complex I could be built from the crystal structures of subcomplexes using the EM envelope described here...
  6. ncbi request reprint Projection structure of the membrane domain of Escherichia coli respiratory complex I at 8 A resolution
    Ekaterina A Baranova
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    J Mol Biol 366:140-54. 2007
    ....
  7. ncbi request reprint Substrate-induced conformational change in bacterial complex I
    Aygun A Mamedova
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:23830-6. 2004
    ..The enzyme retains its L-shape in the presence of NADH, but exhibits a significantly more open or expanded structure both in the peripheral arm and, unexpectedly, in the membrane domain also...
  8. ncbi request reprint Respiratory complex I: mechanistic and structural insights provided by the crystal structure of the hydrophilic domain
    Leonid A Sazanov
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK sazanov mrc dunn cam ac uk
    Biochemistry 46:2275-88. 2007
    ..In this review, novel mechanistic implications of the structure are discussed, and the effects of many known mutations of complex I subunits are interpreted in a structural context...
  9. ncbi request reprint Identification of a novel subunit of respiratory complex I from Thermus thermophilus
    Philip Hinchliffe
    Medical Research Council, Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochemistry 45:4413-20. 2006
    ..thermophilus. Our data show that, contrary to previous assumptions, bacterial complex I can contain proteins in addition to a "core" complement of 14 subunits...
  10. pmc Crystal structure of the entire respiratory complex I
    Rozbeh Baradaran
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Nature 494:443-8. 2013
    ....
  11. doi request reprint Chemical and NADH-induced, ROS-dependent, cross-linking between subunits of complex I from Escherichia coli and Thermus thermophilus
    John M Berrisford
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Biochemistry 47:10262-70. 2008
    ..Our observations suggest that oxidative damage to complex I in vivo may include not only side-chain modifications but also protein cross-linking and degradation...
  12. ncbi request reprint Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I
    Ekaterina A Baranova
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    J Struct Biol 159:238-42. 2007
    ..This indicates that coupling mechanism of complex I is likely to involve long range conformational changes...
  13. doi request reprint Structure of the membrane domain of respiratory complex I
    Rouslan G Efremov
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Nature 476:414-20. 2011
    ..The structure indicates that proton translocation in complex I, uniquely, involves coordinated conformational changes in six symmetrical structural elements...
  14. doi request reprint Structure of Escherichia coli OmpF porin from lipidic mesophase
    Rouslan G Efremov
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    J Struct Biol 178:311-8. 2012
    ..Presence of ordered aliphatic chains close to a positively charged area on the porin surface suggests a position for a lipopolysaccharide binding site on the surface of the major E. coli porins...
  15. ncbi request reprint The location of NuoL and NuoM subunits in the membrane domain of the Escherichia coli complex I: implications for the mechanism of proton pumping
    Peter J Holt
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:43114-20. 2003
    ..This is consistent with proposals that the mechanism of proton pumping by complex I is likely to involve long range conformational changes...
  16. pmc Evolution of respiratory complex I: "supernumerary" subunits are present in the alpha-proteobacterial enzyme
    Chui Ying Yip
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    J Biol Chem 286:5023-33. 2011
    ..It also provides further confirmation that α-proteobacteria are the closest extant relatives of mitochondria...
  17. doi request reprint Respiratory complex I: 'steam engine' of the cell?
    Rouslan G Efremov
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Curr Opin Struct Biol 21:532-40. 2011
    ..The proposed mechanism of coupling between electron transfer and proton translocation involves long-range conformational changes, coordinated in part by a long α-helix, akin to the coupling rod of a steam engine...