I L Sargent

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. ncbi request reprint NK cells and human pregnancy--an inflammatory view
    Ian L Sargent
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, UK, OX3 9DU
    Trends Immunol 27:399-404. 2006
  2. pmc Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis
    Rebecca A Dragovic
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
    Nanomedicine 7:780-8. 2011
  3. ncbi request reprint Immunoregulation in normal pregnancy and pre-eclampsia: an overview
    Ian L Sargent
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK
    Reprod Biomed Online 14:111-7. 2007
  4. ncbi request reprint Immunoregulation in normal pregnancy and pre-eclampsia: an overview
    Ian L Sargent
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK
    Reprod Biomed Online 13:680-6. 2006
  5. ncbi request reprint sHLA-G production by human IVF embryos: can it be measured reliably?
    Ian Sargent
    Oxford Fertility Unit, Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, UK
    J Reprod Immunol 75:128-32. 2007
  6. ncbi request reprint Trophoblast deportation and the maternal inflammatory response in pre-eclampsia
    I L Sargent
    Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, OX3 9DU Oxford, UK
    J Reprod Immunol 59:153-60. 2003
  7. ncbi request reprint NK cells and pre-eclampsia
    I L Sargent
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
    J Reprod Immunol 76:40-4. 2007
  8. pmc Manipulation of CD98 expression affects both trophoblast cell fusion and amino acid transport activity during syncytialization of human placental BeWo cells
    Yoshiki Kudo
    Department of Human Anatomy and Genetics, University of Oxford, Oxford OX1 3QX and Nuffield Department of Obstetrics and Gynecology, John Radcliffe Hospital, Oxford, UK
    J Physiol 550:3-9. 2003
  9. ncbi request reprint Effect of cytokines and growth factors on the secretion of inhibin A, activin A and follistatin by term placental villous trophoblasts in culture
    A Mohan
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Eur J Endocrinol 145:505-11. 2001
  10. ncbi request reprint Flow cytometric measurement of intracellular Th1 and Th2 cytokine production by human villous and extravillous cytotrophoblast
    G P Sacks
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK
    Placenta 22:550-9. 2001

Collaborators

Detail Information

Publications47

  1. ncbi request reprint NK cells and human pregnancy--an inflammatory view
    Ian L Sargent
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, UK, OX3 9DU
    Trends Immunol 27:399-404. 2006
    ..We suggest novel ways in which the trophoblast might stimulate the maternal systemic inflammatory response, and how dysfunctional NK-cell activation could result in the maternal syndrome of pre-eclampsia...
  2. pmc Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis
    Rebecca A Dragovic
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
    Nanomedicine 7:780-8. 2011
    ..By combining NTA with fluorescence measurement we have demonstrated that vesicles can be labeled with specific antibody-conjugated quantum dots, allowing their phenotype to be determined...
  3. ncbi request reprint Immunoregulation in normal pregnancy and pre-eclampsia: an overview
    Ian L Sargent
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK
    Reprod Biomed Online 14:111-7. 2007
    ..There is evidence that such trophoblast debris interacts with maternal leukocytes and endothelial cells to stimulate the release of proinflammatory cytokines, which could then trigger the maternal disease...
  4. ncbi request reprint Immunoregulation in normal pregnancy and pre-eclampsia: an overview
    Ian L Sargent
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK
    Reprod Biomed Online 13:680-6. 2006
    ..There is evidence that such trophoblast debris interacts with maternal leukocytes and endothelial cells to stimulate the release of proinflammatory cytokines, which could then trigger the maternal disease...
  5. ncbi request reprint sHLA-G production by human IVF embryos: can it be measured reliably?
    Ian Sargent
    Oxford Fertility Unit, Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, UK
    J Reprod Immunol 75:128-32. 2007
    ..Further collaboration between the research groups involved is required to establish robust reproducible systems that function identically in all laboratories...
  6. ncbi request reprint Trophoblast deportation and the maternal inflammatory response in pre-eclampsia
    I L Sargent
    Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, OX3 9DU Oxford, UK
    J Reprod Immunol 59:153-60. 2003
    ..It may also contribute to the suppression of Th1 responses seen in pregnancy...
  7. ncbi request reprint NK cells and pre-eclampsia
    I L Sargent
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
    J Reprod Immunol 76:40-4. 2007
    ..Aberrant NK cell activation both locally in the decidua and systemically in the maternal blood may be the cause of pre-eclampsia...
  8. pmc Manipulation of CD98 expression affects both trophoblast cell fusion and amino acid transport activity during syncytialization of human placental BeWo cells
    Yoshiki Kudo
    Department of Human Anatomy and Genetics, University of Oxford, Oxford OX1 3QX and Nuffield Department of Obstetrics and Gynecology, John Radcliffe Hospital, Oxford, UK
    J Physiol 550:3-9. 2003
    ....
  9. ncbi request reprint Effect of cytokines and growth factors on the secretion of inhibin A, activin A and follistatin by term placental villous trophoblasts in culture
    A Mohan
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Eur J Endocrinol 145:505-11. 2001
    ....
  10. ncbi request reprint Flow cytometric measurement of intracellular Th1 and Th2 cytokine production by human villous and extravillous cytotrophoblast
    G P Sacks
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK
    Placenta 22:550-9. 2001
    ..It is proposed that other cytokines (including IL-10 and TNF-alpha) are produced by decidual leukocytes, and not cytotrophoblast, at the maternal-fetal interface...
  11. pmc Tryptophan degradation by human placental indoleamine 2,3-dioxygenase regulates lymphocyte proliferation
    Y Kudo
    Department of Human Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK
    J Physiol 535:207-15. 2001
    ..This mechanism is dependent both on placental indoleamine 2,3-dioxygenase-mediated tryptophan degradation and on tryptophan sensing systems within lymphocytes...
  12. ncbi request reprint An analysis using DNA microarray of the time course of gene expression during syncytialization of a human placental cell line (BeWo)
    Y Kudo
    Department of Human Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK
    Placenta 25:479-88. 2004
    ..Further analysis of this data and expression profiling in general will be able to contribute to understanding the functional basis for the formation of the placental syncytiotrophoblast...
  13. ncbi request reprint Purification and characterization of a complex from placental syncytiotrophoblast microvillous membranes which inhibits the proliferation of human umbilical vein endothelial cells
    Z Kertesz
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, UK
    Placenta 20:71-9. 1999
    ..The major protein bands were as integrins alpha5 and alpha v, dipeptidyl peptidase IV, alpha-actinin, transferrin, transferrin receptor, placental alkaline phosphatase and monoamine oxidase A...
  14. ncbi request reprint Indoleamine 2,3-dioxygenase: distribution and function in the developing human placenta
    Yoshiki Kudo
    Department of Human Anatomy and Genetics, University of Oxford, Oxford, UK
    J Reprod Immunol 61:87-98. 2004
    ..These results suggest the cellular basis of a mechanism present at the human maternal-fetal interface involved in regulating the maternal immune response to conceptus...
  15. ncbi request reprint Hypoxia alters expression and function of syncytin and its receptor during trophoblast cell fusion of human placental BeWo cells: implications for impaired trophoblast syncytialisation in pre-eclampsia
    Yoshiki Kudo
    Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, OX1 3QX, Oxford, UK
    Biochim Biophys Acta 1638:63-71. 2003
    ....
  16. doi request reprint Review: Does size matter? Placental debris and the pathophysiology of pre-eclampsia
    C W G Redman
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, UK
    Placenta 33:S48-54. 2012
    ..We speculate whether changes not only in the numbers, but also in the size (beneficial syncytiotrophoblast exosomes and harmful microvesicles) might be important in the maternal syndrome of pre-eclampsia...
  17. ncbi request reprint Pre-eclampsia, the placenta and the maternal systemic inflammatory response--a review
    C W G Redman
    Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Placenta 24:S21-7. 2003
    ..Hence the MSIR, by causing maternal insulin resistance, may have substantial foetal advantages so long as it is not too severe...
  18. pmc Monocytes are primed to produce the Th1 type cytokine IL-12 in normal human pregnancy: an intracellular flow cytometric analysis of peripheral blood mononuclear cells
    G P Sacks
    Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK
    Clin Exp Immunol 131:490-7. 2003
    ..Furthermore, these data support the hypothesis that components of maternal innate immunity are activated in normal pregnancy...
  19. ncbi request reprint Caveolin-1 and lipid rafts in confluent BeWo trophoblasts: evidence for Rock-1 association with caveolin-1
    F Rashid-Doubell
    Nuffield Department of Obstetrics and Gynaecology, Women s Centre, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
    Placenta 28:139-51. 2007
    ....
  20. doi request reprint Vitamins C and E inhibit apoptosis of cultured human term placenta trophoblast
    D S Tannetta
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
    Placenta 29:680-90. 2008
    ..However, further work is required to determine whether antioxidant vitamins interfere with normal fusion processes...
  21. doi request reprint Placental stress and pre-eclampsia: a revised view
    C W G Redman
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Placenta 30:S38-42. 2009
    ..These may also contribute to the final inflammatory syndrome. We propose a modified version of the two-stage model for pre-eclampsia...
  22. ncbi request reprint Microparticles and immunomodulation in pregnancy and pre-eclampsia
    C W G Redman
    University of Oxford, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford OX3 9DU, UK
    J Reprod Immunol 76:61-7. 2007
    ..Deletion of activate T cells which express Fas ligand by Fas-expressing exosomes derived from trophoblast may contribute to immunoregulation necessary for normal pregnancy...
  23. pmc Cell populations in human early pregnancy decidua: characterization and isolation of large granular lymphocytes by flow cytometry
    P M Starkey
    Harris Birthright Centre for Perinatal Medicine, Nuffield Dept of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, Oxford
    Immunology 65:129-34. 1988
    ..The possible role of decidual LGL in modulating placental development is discussed...
  24. ncbi request reprint Inflammation and pre-eclampsia
    A M Borzychowski
    Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Semin Fetal Neonatal Med 11:309-16. 2006
    ..While clinical management of pre-eclampsia does not currently include anti-inflammatory agents, current research is focusing on ways to reduce inflammation and oxidative stress...
  25. ncbi request reprint The role of pregnancy-specific beta-1 glycoprotein (SP1) in assessing the human blastocyst quality in vitro
    R R Saith
    IVF Unit, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Hum Reprod 11:1038-42. 1996
    ..The inconsistent pattern of secretion suggests that at this early stage of development, SP1 secretion is probably not sufficiently defined to allow any differences in levels to be reflected by the morphological grading...
  26. ncbi request reprint Endothelial cells and peripheral blood mononuclear cells are a potential source of extraplacental activin a in preeclampsia
    D S Tannetta
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
    J Clin Endocrinol Metab 88:5995-6001. 2003
    ..The pathological significance of increased activin A in preeclampsia is unknown, although it may have a role in the mechanisms underlying endothelium dysfunction...
  27. ncbi request reprint Does 'soluble' HLA-G really exist? Another twist to the tale
    I L Sargent
    Nuffield Department of Obstetrics and Gynaecology, Oxford University, John Radcliffe Hospital, Headington, Oxford, UK
    Mol Hum Reprod 11:695-8. 2005
    ..The source of the soluble HLA-G may not matter from a diagnostic perspective, but these findings, if confirmed, have important implications for our understanding of the biology of HLA-G...
  28. ncbi request reprint The pathogenesis of pre-eclampsia
    C W Redman
    Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, United Kingdom, OX3 9DU UK
    Gynecol Obstet Fertil 29:518-22. 2001
    ..Such stress would be most likely with spiral artery disease either from deficient placentation or acute atherosis, In this model, deficient placentation is not the cause of pre-eclampsia but a powerfully predisposing condition...
  29. doi request reprint The effect of labour and placental separation on the shedding of syncytiotrophoblast microparticles, cell-free DNA and mRNA in normal pregnancy and pre-eclampsia
    A Reddy
    Nuffield Department of Obstetrics and Gynaecology, Level 3, Women s Centre, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Placenta 29:942-9. 2008
    ..The enhanced shedding of STBM and CRH mRNA in pre-eclampsia labour may have a role in cases of postpartum worsening of pre-eclampsia...
  30. doi request reprint Circulating microparticles in normal pregnancy and pre-eclampsia
    C W G Redman
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Placenta 29:S73-7. 2008
    ..However inhibitory activity has also been ascribed to trophoblast derived exosomes. In vitro, they down-regulate T cell activity, a T cell change that has been repeatedly observed, ex vivo, during normal pregnancy...
  31. ncbi request reprint Little evidence of HLA-G mRNA polymorphism in Caucasian or Afro-Caribbean populations
    D R Bainbridge
    Nuffield Department of Obstetrics and Gynaecology, Oxford University, The John Radcliffe Hospital, United Kingdom
    J Immunol 163:2023-7. 1999
    ..The monomorphic nature of HLA-G may allow trophoblast to carry out the immunological functions of class I-bearing tissues without compromising successful pregnancy...
  32. pmc Downregulation of caveolin-1 enhances fusion of human BeWo choriocarcinoma cells
    Gavin P Collett
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Oxford, United Kingdom
    PLoS ONE 5:e10529. 2010
    ..Caveolin-1 has been shown to be expressed in human villous cytotrophoblast and to be downregulated during fusion into syncytiotrophoblast but it is unclear whether it plays a role in this process...
  33. doi request reprint Immunology of pre-eclampsia
    Christopher W G Redman
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, UK
    Am J Reprod Immunol 63:534-43. 2010
    ..For the first time, the pathogenesis of pre-eclampsia can be related to defined immune mechanisms that are appropriate to the fetomaternal frontier. Now, the challenge is to prove the detail...
  34. ncbi request reprint Latest advances in understanding preeclampsia
    Christopher W Redman
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Science 308:1592-4. 2005
    ..This brief review on preeclampsia does not cover all aspects of this intriguing condition but focuses on some new and interesting findings...
  35. ncbi request reprint Changes in systemic type 1 and type 2 immunity in normal pregnancy and pre-eclampsia may be mediated by natural killer cells
    Angela M Borzychowski
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, UK
    Eur J Immunol 35:3054-63. 2005
    ..001 and p <0.01, respectively) and pre-eclamptic women (p <0.05). These results confirm that immunoregulation occurs in pregnancy, but suggest a dominant role of the innate rather than the adaptive immune system...
  36. ncbi request reprint Differential effect of cross-linking the CD98 heavy chain on fusion and amino acid transport in the human placental trophoblast (BeWo) cell line
    Paola Dalton
    Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Biochim Biophys Acta 1768:401-10. 2007
    ..The results thus suggest that the function of CD98 in cell fusion is distinct from its role in cellular nutrient delivery...
  37. ncbi request reprint Quantifying the syncytialisation of human placental trophoblast BeWo cells grown in vitro
    Yoshiki Kudo
    Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Biochim Biophys Acta 1640:25-31. 2003
    ..This assay should prove useful in the investigation of the molecular mechanisms involved in trophoblast syncytialisation...
  38. ncbi request reprint Matrix metalloprotease-9, placental syncytiotrophoblast and the endothelial dysfunction of pre-eclampsia
    C A De Jager
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, UK
    Placenta 24:84-91. 2003
    ..Protease inhibitors caused a small reversal of the effects of STBM on morphology and no effect on inhibition of proliferation. We conclude that the effect of STBM on endothelial cells is unlikely to be caused by intrinsic proteases...
  39. ncbi request reprint Antiendometrial antibodies in endometriosis measured by an enzyme-linked immunosorbent assay before and after treatment with danazol and nafarelin
    S H Kennedy
    Nuffield Department of Obstetrics and Gynecology, University of Oxford, John Radcliffe Hospital, United Kingdom
    Obstet Gynecol 75:914-8. 1990
    ..001) (danazol group: P = .091). Our data suggest that in relation to antiendometrial antibodies, nafarelin has a suppressive effect which has previously been ascribed only to danazol...
  40. ncbi request reprint Preeclampsia and the systemic inflammatory response
    Christopher W G Redman
    Department of Obstetric Medicine, University of Oxford, Oxford, UK
    Semin Nephrol 24:565-70. 2004
    ..The clinical implications of these concepts are discussed...
  41. ncbi request reprint Systemic inflammatory priming in normal pregnancy and preeclampsia: the role of circulating syncytiotrophoblast microparticles
    Sarah J Germain
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
    J Immunol 178:5949-56. 2007
    ..Circulating STBMs bind to monocytes and stimulate the production of inflammatory cytokines. It is concluded that they are potential contributors to altered systemic inflammatory responsiveness in pregnancy and pre-eclampsia...
  42. ncbi request reprint Membrane trafficking of CD98 and its ligand galectin 3 in BeWo cells--implication for placental cell fusion
    Paola Dalton
    Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
    FEBS J 274:2715-27. 2007
    ....
  43. ncbi request reprint Adrenomedullin: multiple functions in human pregnancy
    Caroline Wilson
    Nuffield Department of Obstetrics and Gynaecology, The Women s Centre, John Radcliffe Hospital, Oxford, UK
    Angiogenesis 7:203-12. 2004
    ..However, most studies have focused on measuring adrenomedullin levels and studying its expression as well as that of its receptors. More functional studies are now required to elucidate the underlying mechanisms involved...
  44. ncbi request reprint Differential expression of alternatively spliced transcripts of HLA-G in human preimplantation embryos and inner cell masses
    Yuan Q Yao
    Oxford Fertility Unit, Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
    J Immunol 175:8379-85. 2005
    ..Therefore, we have investigated whether down-regulation of HLA-G first occurs in the inner cell mass (precursor fetal cells) of the blastocyst and, in support of this concept, have shown the absence HLA-G1 and -G5 protein and mRNA...
  45. ncbi request reprint Immunological properties of human decidual macrophages--a possible role in intrauterine immunity
    Uma Singh
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
    Reproduction 129:631-7. 2005
    ....
  46. ncbi request reprint Heparin-binding epidermal growth factor and its receptor ErbB4 mediate implantation of the human blastocyst
    Katya Chobotova
    Nuffield Department of Obstetrics and Gynaecology, The Women s Centre, Level 3, John Radcliffe Hospital, University of Oxford, Headington, Oxford, OX3 9DU, UK
    Mech Dev 119:137-44. 2002
    ..Our data lead the way for further dissection of the molecular mechanisms of implantation of the human embryo, and have implications for infertility, in vitro fertilization and contraception...
  47. ncbi request reprint Decreased tryptophan catabolism by placental indoleamine 2,3-dioxygenase in preeclampsia
    Yoshiki Kudo
    Department of Human Anatomy and Genetics, University of Oxford, United Kingdom
    Am J Obstet Gynecol 188:719-26. 2003
    ..Therefore, we studied tryptophan metabolism in pregnant women, with or without preeclampsia, as well as expression and function of placental indoleamine 2,3-dioxygenase...