Gopal P Sapkota

Summary

Affiliation: University of Dundee
Country: UK

Publications

  1. pmc Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome
    Gopal P Sapkota
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 362:481-90. 2002
  2. pmc Ionizing radiation induces ataxia telangiectasia mutated kinase (ATM)-mediated phosphorylation of LKB1/STK11 at Thr-366
    Gopal P Sapkota
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U K
    Biochem J 368:507-16. 2002
  3. doi request reprint Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-β pathway
    David L Bruce
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, UK
    Cell Signal 24:1999-2006. 2012
  4. pmc OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Lina Herhaus
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5 EH, Scotland, UK
    Nat Commun 4:2519. 2013
  5. pmc The TGFβ-induced phosphorylation and activation of p38 mitogen-activated protein kinase is mediated by MAP3K4 and MAP3K10 but not TAK1
    Gopal P Sapkota
    MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Open Biol 3:130067. 2013
  6. pmc USP11 augments TGFβ signalling by deubiquitylating ALK5
    Mazin A Al-Salihi
    Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow St, Dundee DD1 5EH, UK
    Open Biol 2:120063. 2012
  7. doi request reprint Phosphatases in SMAD regulation
    David L Bruce
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, DD1 5EH Dundee, Scotland, UK
    FEBS Lett 586:1897-905. 2012
  8. pmc Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase
    Pauline Douglas
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive, Calgary, AB, T2N 4N1, Canada
    Biochem J 368:243-51. 2002

Collaborators

Detail Information

Publications8

  1. pmc Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome
    Gopal P Sapkota
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 362:481-90. 2002
    ....
  2. pmc Ionizing radiation induces ataxia telangiectasia mutated kinase (ATM)-mediated phosphorylation of LKB1/STK11 at Thr-366
    Gopal P Sapkota
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U K
    Biochem J 368:507-16. 2002
    ..These observations provide the first link between ATM and LKB1 and suggest that ATM could regulate LKB1...
  3. doi request reprint Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-β pathway
    David L Bruce
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, UK
    Cell Signal 24:1999-2006. 2012
    ..We propose that PP5 regulates the TGFβ pathway in MEFs by regulating the expression of SMAD3 protein levels...
  4. pmc OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Lina Herhaus
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5 EH, Scotland, UK
    Nat Commun 4:2519. 2013
    ..Our findings uncover a signal-induced phosphorylation-dependent recruitment of OTUB1 to its target in the TGFβ pathway. ..
  5. pmc The TGFβ-induced phosphorylation and activation of p38 mitogen-activated protein kinase is mediated by MAP3K4 and MAP3K10 but not TAK1
    Gopal P Sapkota
    MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Open Biol 3:130067. 2013
    ....
  6. pmc USP11 augments TGFβ signalling by deubiquitylating ALK5
    Mazin A Al-Salihi
    Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow St, Dundee DD1 5EH, UK
    Open Biol 2:120063. 2012
    ..Central to TGFβ pathway signalling in early embryogenesis and carcinogenesis is TGFβ-induced epithelial to mesenchymal transition. USP11 depletion results in inhibition of TGFβ-induced epithelial to mesenchymal transition...
  7. doi request reprint Phosphatases in SMAD regulation
    David L Bruce
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, DD1 5EH Dundee, Scotland, UK
    FEBS Lett 586:1897-905. 2012
    ..Here we provide an overview of the SMAD regulation by different protein phosphatases and review the evidence supporting each phosphatase as a candidate SMAD-phosphatase...
  8. pmc Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase
    Pauline Douglas
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive, Calgary, AB, T2N 4N1, Canada
    Biochem J 368:243-51. 2002
    ..Moreover, we show that DNA-PKcs is phosphorylated in vivo at Thr2609, Ser2612, Thr2638 and Thr2647 in okadaic acid-treated human cells. We propose that phosphorylation of these sites may play an important role in DNA-PK function...