Gopal P Sapkota

Summary

Affiliation: University of Dundee
Country: UK

Publications

  1. pmc Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome
    Gopal P Sapkota
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 362:481-90. 2002
  2. pmc Ionizing radiation induces ataxia telangiectasia mutated kinase (ATM)-mediated phosphorylation of LKB1/STK11 at Thr-366
    Gopal P Sapkota
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U K
    Biochem J 368:507-16. 2002
  3. pmc Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization
    Lina Herhaus
    MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Sci Signal 8:ra35. 2015
  4. pmc OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Lina Herhaus
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5 EH, Scotland, UK
    Nat Commun 4:2519. 2013
  5. doi request reprint Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-β pathway
    David L Bruce
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, UK
    Cell Signal 24:1999-2006. 2012
  6. pmc USP15 targets ALK3/BMPR1A for deubiquitylation to enhance bone morphogenetic protein signalling
    Lina Herhaus
    Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dow St, Dundee DD1 5EH, UK
    Open Biol 4:140065. 2014
  7. pmc The TGFβ-induced phosphorylation and activation of p38 mitogen-activated protein kinase is mediated by MAP3K4 and MAP3K10 but not TAK1
    Gopal P Sapkota
    MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Open Biol 3:130067. 2013
  8. pmc Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
    Alejandro Rojas-Fernandez
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, United Kingdom
    Sci Rep 5:9811. 2015
  9. pmc Protein associated with SMAD1 (PAWS1/FAM83G) is a substrate for type I bone morphogenetic protein receptors and modulates bone morphogenetic protein signalling
    Janis Vogt
    MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dow St, Dundee DD1 5EH, UK
    Open Biol 4:130210. 2014
  10. pmc USP11 augments TGFβ signalling by deubiquitylating ALK5
    Mazin A Al-Salihi
    Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow St, Dundee DD1 5EH, UK
    Open Biol 2:120063. 2012

Collaborators

Detail Information

Publications13

  1. pmc Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome
    Gopal P Sapkota
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 362:481-90. 2002
    ....
  2. pmc Ionizing radiation induces ataxia telangiectasia mutated kinase (ATM)-mediated phosphorylation of LKB1/STK11 at Thr-366
    Gopal P Sapkota
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U K
    Biochem J 368:507-16. 2002
    ..These observations provide the first link between ATM and LKB1 and suggest that ATM could regulate LKB1...
  3. pmc Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization
    Lina Herhaus
    MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Sci Signal 8:ra35. 2015
    ..Our findings indicate that the activity of CK2 is necessary for the nuclear translocation and subsequent function of OTUB1 in DNA damage repair. ..
  4. pmc OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Lina Herhaus
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5 EH, Scotland, UK
    Nat Commun 4:2519. 2013
    ..Our findings uncover a signal-induced phosphorylation-dependent recruitment of OTUB1 to its target in the TGFβ pathway. ..
  5. doi request reprint Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-β pathway
    David L Bruce
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, UK
    Cell Signal 24:1999-2006. 2012
    ..We propose that PP5 regulates the TGFβ pathway in MEFs by regulating the expression of SMAD3 protein levels...
  6. pmc USP15 targets ALK3/BMPR1A for deubiquitylation to enhance bone morphogenetic protein signalling
    Lina Herhaus
    Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dow St, Dundee DD1 5EH, UK
    Open Biol 4:140065. 2014
    ..We also show that loss of USP15 expression from mouse myoblast cells inhibits BMP-induced osteoblast differentiation. Furthermore, USP15 modulates BMP-induced phosphorylation of SMAD1 and transcription during Xenopus embryogenesis. ..
  7. pmc The TGFβ-induced phosphorylation and activation of p38 mitogen-activated protein kinase is mediated by MAP3K4 and MAP3K10 but not TAK1
    Gopal P Sapkota
    MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Open Biol 3:130067. 2013
    ....
  8. pmc Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
    Alejandro Rojas-Fernandez
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, United Kingdom
    Sci Rep 5:9811. 2015
    ..Our unique approach has the potential to expedite studies on transcription of any gene in the context of its native chromatin landscape in somatic cells, allowing for robust high-throughput chemical and genetic screens. ..
  9. pmc Protein associated with SMAD1 (PAWS1/FAM83G) is a substrate for type I bone morphogenetic protein receptors and modulates bone morphogenetic protein signalling
    Janis Vogt
    MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dow St, Dundee DD1 5EH, UK
    Open Biol 4:130210. 2014
    ..We also demonstrate that PAWS1 regulates the expression of several non-BMP target genes, suggesting roles for PAWS1 beyond the BMP pathway. ..
  10. pmc USP11 augments TGFβ signalling by deubiquitylating ALK5
    Mazin A Al-Salihi
    Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow St, Dundee DD1 5EH, UK
    Open Biol 2:120063. 2012
    ..Central to TGFβ pathway signalling in early embryogenesis and carcinogenesis is TGFβ-induced epithelial to mesenchymal transition. USP11 depletion results in inhibition of TGFβ-induced epithelial to mesenchymal transition...
  11. pmc The emerging roles of deubiquitylating enzymes (DUBs) in the TGFβ and BMP pathways
    Lina Herhaus
    MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH Scotland, United Kingdom
    Cell Signal 26:2186-92. 2014
    ..This review will provide a concise summary of our current understanding of how DUBs regulate TGFβ signalling...
  12. doi request reprint Phosphatases in SMAD regulation
    David L Bruce
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, DD1 5EH Dundee, Scotland, UK
    FEBS Lett 586:1897-905. 2012
    ..Here we provide an overview of the SMAD regulation by different protein phosphatases and review the evidence supporting each phosphatase as a candidate SMAD-phosphatase...
  13. pmc Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase
    Pauline Douglas
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive, Calgary, AB, T2N 4N1, Canada
    Biochem J 368:243-51. 2002
    ..Moreover, we show that DNA-PKcs is phosphorylated in vivo at Thr2609, Ser2612, Thr2638 and Thr2647 in okadaic acid-treated human cells. We propose that phosphorylation of these sites may play an important role in DNA-PK function...