Kei Sakamoto

Summary

Affiliation: University of Dundee
Country: UK

Publications

  1. pmc Deficiency of LKB1 in skeletal muscle prevents AMPK activation and glucose uptake during contraction
    Kei Sakamoto
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    EMBO J 24:1810-20. 2005
  2. pmc Emerging role for AS160/TBC1D4 and TBC1D1 in the regulation of GLUT4 traffic
    Kei Sakamoto
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow St, Dundee, DD1 5EH, UK
    Am J Physiol Endocrinol Metab 295:E29-37. 2008
  3. pmc Deficiency of LKB1 in heart prevents ischemia-mediated activation of AMPKalpha2 but not AMPKalpha1
    Kei Sakamoto
    Medical Research Council Protein Phosphorylation Unit, School of Life Sciences, Univ of Dundee, Dow St, Dundee DD1 5EH, UK
    Am J Physiol Endocrinol Metab 290:E780-8. 2006
  4. doi request reprint Silencing metabolic disorders by novel SIRT1 activators
    Kei Sakamoto
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, UK
    Cell Metab 7:3-4. 2008
  5. ncbi request reprint Activity of LKB1 and AMPK-related kinases in skeletal muscle: effects of contraction, phenformin, and AICAR
    Kei Sakamoto
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Scotland, UK
    Am J Physiol Endocrinol Metab 287:E310-7. 2004
  6. ncbi request reprint Role of the PDK1-PKB-GSK3 pathway in regulating glycogen synthase and glucose uptake in the heart
    Alfonso Mora
    MRC Protein Phosphorylation Unit, School of Life Sciences, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland
    FEBS Lett 579:3632-8. 2005
  7. doi request reprint Use of Akt inhibitor and a drug-resistant mutant validates a critical role for protein kinase B/Akt in the insulin-dependent regulation of glucose and system A amino acid uptake
    Charlotte J Green
    Division of Molecular Physiology, James Black Centre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 283:27653-67. 2008
  8. pmc How moderate changes in Akt T-loop phosphorylation impact on tumorigenesis and insulin resistance
    Stephan Wullschleger
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Dis Model Mech 4:95-103. 2011
  9. pmc Thr649Ala-AS160 knock-in mutation does not impair contraction/AICAR-induced glucose transport in mouse muscle
    Serge Ducommun
    Medical Rersearch Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Scotland, United Kingdom
    Am J Physiol Endocrinol Metab 302:E1036-43. 2012
  10. ncbi request reprint Evaluation of approaches to generation of tissue-specific knock-in mice
    Jose R Bayascas
    MRC Protein Phosphorylation Unit and School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 281:28772-81. 2006

Detail Information

Publications65

  1. pmc Deficiency of LKB1 in skeletal muscle prevents AMPK activation and glucose uptake during contraction
    Kei Sakamoto
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    EMBO J 24:1810-20. 2005
    ..These studies establish the importance of LKB1 in regulating AMPK activity and cellular energy levels in response to contraction and phenformin...
  2. pmc Emerging role for AS160/TBC1D4 and TBC1D1 in the regulation of GLUT4 traffic
    Kei Sakamoto
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow St, Dundee, DD1 5EH, UK
    Am J Physiol Endocrinol Metab 295:E29-37. 2008
    ....
  3. pmc Deficiency of LKB1 in heart prevents ischemia-mediated activation of AMPKalpha2 but not AMPKalpha1
    Kei Sakamoto
    Medical Research Council Protein Phosphorylation Unit, School of Life Sciences, Univ of Dundee, Dow St, Dundee DD1 5EH, UK
    Am J Physiol Endocrinol Metab 290:E780-8. 2006
    ..They also provide genetic evidence that an alternative upstream kinase can activate AMPKalpha1 in cardiac muscle...
  4. doi request reprint Silencing metabolic disorders by novel SIRT1 activators
    Kei Sakamoto
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, UK
    Cell Metab 7:3-4. 2008
    ..In a recent issue of Nature, Milne et al. (2007) describe novel potent activators of SIRT1, whose administration to insulin-resistant animals improves glucose homeostasis...
  5. ncbi request reprint Activity of LKB1 and AMPK-related kinases in skeletal muscle: effects of contraction, phenformin, and AICAR
    Kei Sakamoto
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Scotland, UK
    Am J Physiol Endocrinol Metab 287:E310-7. 2004
    ..They also suggest that the effects of excercise, phenformin, and AICAR on metabolic processes in muscle may be mediated through activation of AMPK rather than activation of LKB1 or the AMPK-related kinases...
  6. ncbi request reprint Role of the PDK1-PKB-GSK3 pathway in regulating glycogen synthase and glucose uptake in the heart
    Alfonso Mora
    MRC Protein Phosphorylation Unit, School of Life Sciences, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland
    FEBS Lett 579:3632-8. 2005
    ....
  7. doi request reprint Use of Akt inhibitor and a drug-resistant mutant validates a critical role for protein kinase B/Akt in the insulin-dependent regulation of glucose and system A amino acid uptake
    Charlotte J Green
    Division of Molecular Physiology, James Black Centre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 283:27653-67. 2008
    ....
  8. pmc How moderate changes in Akt T-loop phosphorylation impact on tumorigenesis and insulin resistance
    Stephan Wullschleger
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Dis Model Mech 4:95-103. 2011
    ....
  9. pmc Thr649Ala-AS160 knock-in mutation does not impair contraction/AICAR-induced glucose transport in mouse muscle
    Serge Ducommun
    Medical Rersearch Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Scotland, United Kingdom
    Am J Physiol Endocrinol Metab 302:E1036-43. 2012
    ....
  10. ncbi request reprint Evaluation of approaches to generation of tissue-specific knock-in mice
    Jose R Bayascas
    MRC Protein Phosphorylation Unit and School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 281:28772-81. 2006
    ..We discuss the merits and disadvantages of each of the conditional knock-in approaches, along with the applications for which they may be most suited, and suggest how they could be further refined...
  11. doi request reprint Important role of the LKB1-AMPK pathway in suppressing tumorigenesis in PTEN-deficient mice
    Xu Huang
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Biochem J 412:211-21. 2008
    ....
  12. pmc Glucose-6-phosphate-mediated activation of liver glycogen synthase plays a key role in hepatic glycogen synthesis
    Alexander Von Wilamowitz-Moellendorff
    Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, Scotland, U K
    Diabetes 62:4070-82. 2013
    ..These data show that G6P-mediated activation of GYS2 plays a key role in controlling glycogen synthesis and hepatic glucose-G6P flux control and thus whole-body glucose homeostasis. ..
  13. doi request reprint A novel short splice variant of the tumour suppressor LKB1 is required for spermiogenesis
    Mhairi C Towler
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee, Scotland, UK
    Biochem J 416:1-14. 2008
    ..These results identify a previously undetected variant of LKB1, and suggest that it has a crucial role in spermiogenesis and male fertility...
  14. ncbi request reprint AMPK: a key sensor of fuel and energy status in skeletal muscle
    D Grahame Hardie
    Division of Molecular Physiology, University of Dundee, Dundee, Scotland
    Physiology (Bethesda) 21:48-60. 2006
    ..Recent work using a muscle-specific knockout of the upstream kinase, LKB1, has confirmed that the LKB1-->AMPK cascade is the signaling pathway responsible for many of these effects...
  15. pmc Mice with AS160/TBC1D4-Thr649Ala knockin mutation are glucose intolerant with reduced insulin sensitivity and altered GLUT4 trafficking
    Shuai Chen
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Cell Metab 13:68-79. 2011
    ....
  16. pmc Differential effects of palmitate and palmitoleate on insulin action and glucose utilization in rat L6 skeletal muscle cells
    Nikolaos Dimopoulos
    Division of Molecular Physiology, Centre for Interdisciplinary Research, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Biochem J 399:473-81. 2006
    ....
  17. pmc Molecular mechanism by which AMP-activated protein kinase activation promotes glycogen accumulation in muscle
    Roger W Hunter
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, UK
    Diabetes 60:766-74. 2011
    ..The aim of this study was to elucidate the molecular mechanism by which AMPK activation promotes muscle glycogen accumulation...
  18. doi request reprint Counter-modulation of fatty acid-induced pro-inflammatory nuclear factor κB signalling in rat skeletal muscle cells by AMP-activated protein kinase
    Charlotte J Green
    Division of Cell Signalling and Immunology, Sir James Black Centre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Biochem J 435:463-74. 2011
    ....
  19. doi request reprint Allosteric regulation of glycogen synthase controls glycogen synthesis in muscle
    Michale Bouskila
    University of Dundee, Scotland, UK
    Cell Metab 12:456-66. 2010
    ..Our study provides genetic evidence that allosteric activation of GS is the primary mechanism by which insulin promotes muscle glycogen accumulation in vivo...
  20. pmc Mutation in the γ2-subunit of AMP-activated protein kinase stimulates cardiomyocyte proliferation and hypertrophy independent of glycogen storage
    Maengjo Kim
    From the Department of Anesthesiology and Pain Medicine, Mitochondria and Metabolism Center, University of Washington, Seattle M K, L G M, G G, Y Y Y, S C K, J X, W W, R T and MRC Protein Phosphorylation unit, College of Life Sciences, University of Dundee, Dundee, United Kingdom R W H, K S R W H and K S are currently affiliated with Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Park, Bâtiment G, Lausanne, Switzerland
    Circ Res 114:966-75. 2014
    ..Understanding the disease mechanisms of Prkag2 cardiomyopathy is not only beneficial for the patients but also critical to the use of AMP-activated protein kinase as a drug target...
  21. doi request reprint Protor-1 is required for efficient mTORC2-mediated activation of SGK1 in the kidney
    Laura R Pearce
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 436:169-79. 2011
    ..Taken together, these results suggest that Protor-1 may play a role in enabling mTORC2 to efficiently activate SGK1, at least in the kidney...
  22. pmc AMP-activated protein kinase mediates preconditioning in cardiomyocytes by regulating activity and trafficking of sarcolemmal ATP-sensitive K(+) channels
    Andrey Sukhodub
    Maternal and Child Health Sciences, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK
    J Cell Physiol 210:224-36. 2007
    ....
  23. pmc Cellular responses to the metal-binding properties of metformin
    Lisa Logie
    Division of Cardiovascular and Diabetes Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, Scotland, UK
    Diabetes 61:1423-33. 2012
    ..Together, these results suggest that cellular effects of biguanides depend on their metal-binding properties. This link may illuminate a better understanding of the molecular mechanisms enabling antihyperglycemic drug action...
  24. pmc The ancient drug salicylate directly activates AMP-activated protein kinase
    Simon A Hawley
    Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Science 336:918-22. 2012
    ..Our results suggest that AMPK activation could explain some beneficial effects of salsalate and aspirin in humans...
  25. pmc Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance
    Jose R Bayascas
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland
    Mol Cell Biol 28:3258-72. 2008
    ..Our findings reveal how reduced activation of PKB isoforms impinges on downstream signaling pathways, causing diminution of size as well as insulin resistance...
  26. ncbi request reprint Insulin-stimulated glucose uptake does not require p38 mitogen-activated protein kinase in adipose tissue or skeletal muscle
    Sophie Turban
    Division of Molecular Physiology, Medical Research Council Protein Phosphorylation Unit, University of Dundee, Dundee, UK
    Diabetes 54:3161-8. 2005
    ....
  27. pmc Regulation of the NKCC2 ion cotransporter by SPAK-OSR1-dependent and -independent pathways
    Ciaran Richardson
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    J Cell Sci 124:789-800. 2011
    ....
  28. pmc Investigation of LKB1 Ser431 phosphorylation and Cys433 farnesylation using mouse knockin analysis reveals an unexpected role of prenylation in regulating AMPK activity
    Vanessa P Houde
    MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, U K
    Biochem J 458:41-56. 2014
    ..This raises the possibility that LKB1 farnesylation and myristoylation of AMPKβ might promote the interaction and co-localization of these enzymes on a two-dimensional membrane surface and thereby promote efficient activation of AMPK...
  29. pmc Analysis of substrate specificity and cyclin Y binding of PCTAIRE-1 kinase
    Saifeldin N Shehata
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, UK
    Cell Signal 24:2085-94. 2012
    ..Our understanding of the molecular interaction between PCTAIRE-1 and cyclin Y further facilitates future investigation of the functions of PCTAIRE-1 kinase...
  30. ncbi request reprint LKB1-dependent signaling pathways
    Dario R Alessi
    Medical Research Council, Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland
    Annu Rev Biochem 75:137-63. 2006
    ..The work described in this review shows how a study of an obscure cancer syndrome can uncover new and important regulatory pathways, relevant to the understanding of multiple human diseases...
  31. pmc Role that phosphorylation of GSK3 plays in insulin and Wnt signalling defined by knockin analysis
    Edward J McManus
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dundee, Scotland
    EMBO J 24:1571-83. 2005
    ..These results establish the function of Ser21/Ser9 phosphorylation in several processes in which GSK3 inactivation has previously been implicated...
  32. doi request reprint Role of TAPP1 and TAPP2 adaptor binding to PtdIns(3,4)P2 in regulating insulin sensitivity defined by knock-in analysis
    Stephan Wullschleger
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Biochem J 434:265-74. 2011
    ..These observations provide the first genetic evidence to support the notion that binding of TAPP1 and TAPP2 adap-tors to PtdIns(3,4)P(2) function as negative regulators of the insulin and PI3K signalling pathways...
  33. pmc Molecular mechanism of action of metformin: old or new insights?
    Graham Rena
    Cardiovascular and Diabetes Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, UK
    Diabetologia 56:1898-906. 2013
    ..In this review we summarise the recent research developments on the molecular action of metformin. ..
  34. doi request reprint Effect of high-intensity intermittent swimming on postexercise insulin sensitivity in rat epitrochlearis muscle
    Keiichi Koshinaka
    Department of Health and Nutrition, Niigata University of Health and Welfare, 1398 Shimami cho, Kita ku, Niigata City, Niigata 950 3198, Japan
    Metabolism 57:749-56. 2008
    ..Thus, AMPK activation immediately after exercise may not be the only factor that determines the magnitude of the exercise-induced increase in insulin sensitivity in rat epitrochlearis muscle...
  35. ncbi request reprint Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle
    Henning F Kramer
    Section Head, Metabolism, Joslin Diabetes Center, One Joslin Pl, Boston, MA 02215, USA
    Diabetes 55:2067-76. 2006
    ..While Akt and AMPK alpha2 activities are essential for AS160 phosphorylation by insulin and AICAR, respectively, neither kinase is indispensable for the entire effects of contraction on AS160 phosphorylation...
  36. pmc Reduced expression of the murine p85alpha subunit of phosphoinositide 3-kinase improves insulin signaling and ameliorates diabetes
    Franck Mauvais-Jarvis
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Clin Invest 109:141-9. 2002
    ..Furthermore, Pik3r1 heterozygosity protects mice with genetic insulin resistance from developing diabetes. These data suggest that regulation of p85alpha levels may provide a novel therapeutic target for the treatment of type 2 diabetes...
  37. doi request reprint c-Jun NH2-terminal kinase 1 is a critical regulator for the development of gastric cancer in mice
    Wataru Shibata
    Division of Gastroenterology, Institute for Adult Diseases, Asahi Life Foundation, Chiyoda ku, Tokyo, Japan
    Cancer Res 68:5031-9. 2008
    ..Taken together, JNK seems to be involved in both tumor initiation and promotion and may be an attractive target for the prevention of gastric carcinogenesis...
  38. ncbi request reprint The effect of exercise and insulin on AS160 phosphorylation and 14-3-3 binding capacity in human skeletal muscle
    Kirsten F Howlett
    School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria 3125, Australia
    Am J Physiol Endocrinol Metab 294:E401-7. 2008
    ..Insulin increases AS160 phosphorylation and 14-3-3 binding capacity, but prior exercise does not appear to enhance the response to insulin...
  39. ncbi request reprint Insulin promotes glycogen synthesis in the absence of GSK3 phosphorylation in skeletal muscle
    Michale Bouskila
    Am J Physiol Endocrinol Metab 294:E28-35. 2008
    ..This suggests that allosteric regulation of GS by G-6-P may play a key role in insulin-stimulated muscle glycogen synthesis in vivo...
  40. ncbi request reprint Regulation of dishevelled and beta-catenin in rat skeletal muscle: an alternative exercise-induced GSK-3beta signaling pathway
    William G Aschenbach
    Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    Am J Physiol Endocrinol Metab 291:E152-8. 2006
    ..In conclusion, exercise in vivo, but not insulin, increases the association between Dvl and GSK-3beta in skeletal muscle, an event paralleled by beta-catenin dephosphorylation...
  41. ncbi request reprint Exercise regulates Akt and glycogen synthase kinase-3 activities in human skeletal muscle
    Kei Sakamoto
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA, USA
    Biochem Biophys Res Commun 319:419-25. 2004
    ....
  42. ncbi request reprint Overexpression or ablation of JNK in skeletal muscle has no effect on glycogen synthase activity
    Nobuharu Fujii
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Cell Physiol 287:C200-8. 2004
    ..JNK activation is unlikely to be the major mechanism by which contractile activity increases glycogen synthase activity in skeletal muscle...
  43. ncbi request reprint 5' adenosine monophosphate-activated protein kinase, metabolism and exercise
    William G Aschenbach
    Research Division, Joslin Diabetes Center and Harvard Medical School, Boston, Massachusetts, USA
    Sports Med 34:91-103. 2004
    ..This review discusses the putative roles of AMPK in acute and chronic exercise responses, and suggests avenues for future AMPK research in exercise physiology and biochemistry...
  44. ncbi request reprint Akt signaling in skeletal muscle: regulation by exercise and passive stretch
    Kei Sakamoto
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Endocrinol Metab 285:E1081-8. 2003
    ..We conclude that physiological exercise in vivo activates Akt in multiple skeletal muscle fiber types and that mechanical tension may be a part of the mechanism by which contraction activates Akt in fast-twitch muscles...
  45. ncbi request reprint AMP-activated protein kinase activates transcription of the UCP3 and HKII genes in rat skeletal muscle
    James Stoppani
    The John B Pierce Laboratory and Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06519, USA
    Am J Physiol Endocrinol Metab 283:E1239-48. 2002
    ..These data provide evidence that AMPK signaling is linked to the transcriptional regulation of select metabolic genes in skeletal muscle...
  46. ncbi request reprint Invited review: intracellular signaling in contracting skeletal muscle
    Kei Sakamoto
    Research Division, Joslin Diabetes Center, Boston, Massachusetts 02215, USA
    J Appl Physiol 93:369-83. 2002
    ..A fundamental goal of signaling research is to determine the biological consequences of exercise-induced signaling through these molecules, and this review also provides an update of progress in this area...
  47. ncbi request reprint Effect of AICAR treatment on glycogen metabolism in skeletal muscle
    William G Aschenbach
    Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Diabetes 51:567-73. 2002
    ..Rather, the increased glycogen is due to the well-known effects of AICAR to increase glucose uptake...
  48. ncbi request reprint Insulin signaling after exercise in insulin receptor substrate-2-deficient mice
    Kirsten F Howlett
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    Diabetes 51:479-83. 2002
    ..IRS-2 signaling can partially account for the increase in insulin-stimulated phosphotyrosine-associated PI 3-kinase activity after exercise...
  49. ncbi request reprint Role of Akt2 in contraction-stimulated cell signaling and glucose uptake in skeletal muscle
    Kei Sakamoto
    The Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Endocrinol Metab 291:E1031-7. 2006
    ....
  50. ncbi request reprint Insulin-stimulated insulin receptor substrate-2-associated phosphatidylinositol 3-kinase activity is enhanced in human skeletal muscle after exercise
    Kirsten F Howlett
    Center for Physical Activity and Nutrition C PAN, School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria 3125, Australia
    Metabolism 55:1046-52. 2006
    ..In conclusion, in the immediate period after an acute bout of exercise, insulin-stimulated IRS-2 signaling is enhanced in human skeletal muscle...
  51. ncbi request reprint Contraction regulation of Akt in rat skeletal muscle
    Kei Sakamoto
    Research Division, Joslin Diabetes Center and the Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 277:11910-7. 2002
    ..Akt signaling is not necessary for the regulation of glycogen synthase activity in contracting skeletal muscle...
  52. pmc Mechanism of action of A-769662, a valuable tool for activation of AMP-activated protein kinase
    Olga Göransson
    Division for Diabetes, Metabolism and Endocrinology, Department of Experimental Medical Science, Lund University, Lund, Sweden
    J Biol Chem 282:32549-60. 2007
    ..We propose that this direct and specific AMPK activator will be a valuable experimental tool to understand the physiological roles of AMPK...
  53. ncbi request reprint Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle
    Kirsten F Howlett
    School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia
    Diabetes 56:1608-14. 2007
    ..Regulation of AS160 and interaction with 14-3-3 in skeletal muscle are influenced by resistance exercise and insulin but do not fully explain the effect of resistance exercise on whole-body insulin action...
  54. pmc Effect of acute exercise on AMPK signaling in skeletal muscle of subjects with type 2 diabetes: a time-course and dose-response study
    Apiradee Sriwijitkamol
    Texas Diabetes Institute, and Diabetes Division, University of Texas Health Science Center at San Antonio, TX 78207, USA
    Diabetes 56:836-48. 2007
    ..Our findings suggest that obese type 2 diabetic subjects may need to exercise at higher intensity to stimulate the AMPK-AS160 axis to the same level as lean subjects...
  55. ncbi request reprint Cutting edge: The IkappaB kinase (IKK) inhibitor, NEMO-binding domain peptide, blocks inflammatory injury in murine colitis
    Wataru Shibata
    Division of Gastroenterology, Institute for Adult Diseases, Asahi Life Foundation, 1 6 1 Marunouchi, Chiyoda ku, Tokyo 100 0005, Japan
    J Immunol 179:2681-5. 2007
    ..These results indicate that an IKKbeta-targeted NF-kappaB blockade using the NBD peptide could be an attractive therapeutic approach for inflammatory bowel disease...
  56. ncbi request reprint The nephroblastoma overexpressed gene (NOV/ccn3) protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway
    Kei Sakamoto
    Department of Molecular Pathology, Graduate School of Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8549, Japan
    J Biol Chem 277:29399-405. 2002
    ..These results indicate that NOV-Notch1 association exerts a positive effect on Notch signaling and consequently suppresses myogenesis...
  57. doi request reprint Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells
    Kei Sakamoto
    Section of Oral Pathology, Graduate School, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8549, Japan
    J Cell Sci 121:1613-23. 2008
    ....
  58. ncbi request reprint Significance of the fibrous stroma in bone invasion by human gingival squamous cell carcinomas
    Masafumi Ishikuro
    Section of Oral Pathology, Department of Oral Restitution, Graduate School of Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8549 Japan
    Bone 43:621-7. 2008
    ..These results indicate that the fibrous stroma plays critical roles in osteoclastic bone resorption by gingival SCC through the RANKL-dependent pathways...
  59. ncbi request reprint BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines
    Tingjiao Liu
    Section of Oral Pathology, Department of Oral Restitution, Graduate School of Tokyo Medical and Dental University, Tokyo, Japan
    J Cell Physiol 211:728-35. 2007
    ....
  60. ncbi request reprint Differentiation of an ameloblast-lineage cell line (ALC) is induced by Sonic hedgehog signaling
    Satomi Takahashi
    Pulp Biology and Endodontics, Graduate School of Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8549, Japan
    Biochem Biophys Res Commun 353:405-11. 2007
    ..These results suggest that Shh directly promotes ALC differentiation, and that Gli1 may be responsible for the signal mediation of Shh...
  61. ncbi request reprint Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa
    Nobuyuki Kawashima
    Pulp Biology and Endodontics, Graduate School of Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo, 113 8549, Japan
    J Bone Miner Metab 23:123-33. 2005
    ..On the contrary, Myocyte enhancer factor 2C (MEF2C), a myogenic transcriptional factor, was high in Kusa-A1, although no expression of any other myogenic genes was shown...
  62. ncbi request reprint Takayasu myocarditis mediated by cytotoxic T lymphocytes
    Norifumi Takeda
    Department of Cardiovascular Medicine, University of Tokyo Graduate School of Medicine, Bunkyo ku, Tokyo 113 8655
    Intern Med 44:256-60. 2005
    ..Prednisolone administration provided great benefits to cardiac function. These findings suggest that autoimmune cytotoxic mechanisms similar to those in arterial tissue may contribute to cardiac impairment in Takayasu's arteritis...
  63. ncbi request reprint Osteogenic differentiation of the mesenchymal progenitor cells, Kusa is suppressed by Notch signaling
    Kentaro Shindo
    Pulp Biology and Endodontics, Graduate School of Tokyo Medical and Dental University, 1 5 45, Yushima, Bunkyo ku, Tokyo 113 8549, Japan
    Exp Cell Res 290:370-80. 2003
    ..Osteoclasts were induced in the KusaA-derived bone-like tissues, but lacked in the KusaA(NICD)-derived tissues. These results suggest that Notch signaling suppresses the osteoblastic differentiation of mesenchymal progenitor cells...
  64. doi request reprint Deletion of apoptosis signal-regulating kinase 1 attenuates acetaminophen-induced liver injury by inhibiting c-Jun N-terminal kinase activation
    Hayato Nakagawa
    Department of Gastroenterology, University of Tokyo, Hongo, Bunkyo ku, Tokyo
    Gastroenterology 135:1311-21. 2008
    ..We tested the hypothesis that ASK1 was involved in APAP-induced JNK activation and liver injury...
  65. ncbi request reprint Involvement of p38 MAP kinase in not only activation of the phagocyte NADPH oxidase induced by formyl-methionyl-leucyl-phenylalanine but also determination of the extent of the activity
    Kei Sakamoto
    Department of Molecular and Cellular Biochemistry, Graduate School of Medical Sciences, Kyushu University, 3 1 1, Maidashi, Higashi ku, Fukuoka 812 8582
    J Biochem 140:739-45. 2006
    ..These results suggest that p38 MAP kinase is involved not only in activating NADPH oxidase stimulated by fMLP but also in determining the extent of its activity...