H Saibil

Summary

Affiliation: University of London
Country: UK

Publications

  1. ncbi request reprint Challenges at the frontiers of structural biology
    Helen R Saibil
    Helen R Saibil is in the Department of Crystallography, Birkbeck College London, Malet Street, London, WC1E 7HX, United Kingdom
    Nat Struct Biol 9:414-5. 2002
  2. pmc Heritable yeast prions have a highly organized three-dimensional architecture with interfiber structures
    Helen R Saibil
    Crystallography and Institute for Structural and Molecular Biology, Birkbeck College, London WC1E 7HX, United Kingdom
    Proc Natl Acad Sci U S A 109:14906-11. 2012
  3. pmc ATP-triggered conformational changes delineate substrate-binding and -folding mechanics of the GroEL chaperonin
    Daniel K Clare
    Crystallography and Institute of Structural and Molecular Biology, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK
    Cell 149:113-23. 2012
  4. pmc Motor mechanism for protein threading through Hsp104
    Petra Wendler
    Department of Crystallography, Birkbeck College, London, UK
    Mol Cell 34:81-92. 2009
  5. pmc Topologies of a substrate protein bound to the chaperonin GroEL
    Nadav Elad
    Department of Crystallography, Birkbeck College London, Malet Street, London WC1E 7HX, UK
    Mol Cell 26:415-26. 2007
  6. pmc Globular tetramers of beta(2)-microglobulin assemble into elaborate amyloid fibrils
    Helen E White
    Department of Crystallography and Institute of Structural and Molecular Biology, Birkbeck College, London, UK
    J Mol Biol 389:48-57. 2009
  7. pmc Atypical AAA+ subunit packing creates an expanded cavity for disaggregation by the protein-remodeling factor Hsp104
    Petra Wendler
    Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, UK
    Cell 131:1366-77. 2007
  8. doi request reprint Chaperone machines in action
    Helen R Saibil
    Department of Crystallography and Institute of Structural Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, UK
    Curr Opin Struct Biol 18:35-42. 2008
  9. ncbi request reprint Molecular chaperones: containers and surfaces for folding, stabilising or unfolding proteins
    H Saibil
    Department of Crystallography, Birkbeck College, London, WC1E 7HX, UK
    Curr Opin Struct Biol 10:251-8. 2000
  10. ncbi request reprint Conformational changes studied by cryo-electron microscopy
    H R Saibil
    Crystallography Department, Birkbeck College, Malet Street, London WC1E 7HX, UK
    Nat Struct Biol 7:711-4. 2000

Collaborators

  • A L Horwich
  • Carol V Robinson
  • Susan Lindquist
  • Mikhail Eltsov
  • Jens Tyedmers
  • Daniel Castaño-Diez
  • Achilleas S Frangakis
  • Elena V Orlova
  • M Haslbeck
  • C M Dobson
  • Gabriel Waksman
  • Timothy R Dafforn
  • Daniel K Clare
  • George W Farr
  • Petra Wendler
  • Helen E White
  • Nadav Elad
  • Sarah J Tilley
  • Luchun Wang
  • Scott Stagg
  • Joel Quispe
  • Remi Fronzes
  • Eva Schäfer
  • Tobias Krojer
  • James Shorter
  • Natalya Lukoyanova
  • Celia Plisson
  • Sara Cohen-Krausz
  • M Howard Tattum
  • Neil A Ranson
  • Athanasios Ignatiou
  • Christopher K Kennaway
  • Shaoxia Chen
  • B Trevor Sewell
  • Corinne J Smith
  • Jose L Jimenez
  • Maya Topf
  • Daven Vasishtan
  • Thomas R Jahn
  • Julie L Hodgkinson
  • David Snead
  • Sheena E Radford
  • Walraj S Gosal
  • Shirley Müller
  • Michael Ehrmann
  • Justyna Sawa
  • Tim Clausen
  • Anil G Cashikar
  • Brent Gowen
  • John Collinge
  • Michael A Harrison
  • Titus M Franzmann
  • Azadeh Khalili-Shirazi
  • Anthony R Clarke
  • Christopher W Wharton
  • Thusnelda Stromer
  • Graham S Jackson
  • Kanjana Thumanu
  • David Houldershaw
  • John B C Findlay
  • Johannes Buchner
  • Saskia M van der Vies
  • Patrick J Bakkes
  • Malcolm A Finbow
  • Harm van Heerikhuizen
  • Justin L P Benesch
  • Peter W Andrew
  • Helen R Saibi
  • Robert J C Gilbert
  • Ulrich Gohlke
  • Nicholas H Keep
  • Kavita Khubchandani-Aswani
  • Robert B Best
  • Alan M Roseman
  • Barbara M F Pearse
  • Catherine A Sims
  • Helen Kent
  • Lin Zhang
  • Tapan K Chaudhuri
  • Wayne A Fenton
  • Ewan J Nettleton
  • Mario Bouchard

Detail Information

Publications32

  1. ncbi request reprint Challenges at the frontiers of structural biology
    Helen R Saibil
    Helen R Saibil is in the Department of Crystallography, Birkbeck College London, Malet Street, London, WC1E 7HX, United Kingdom
    Nat Struct Biol 9:414-5. 2002
    ....
  2. pmc Heritable yeast prions have a highly organized three-dimensional architecture with interfiber structures
    Helen R Saibil
    Crystallography and Institute for Structural and Molecular Biology, Birkbeck College, London WC1E 7HX, United Kingdom
    Proc Natl Acad Sci U S A 109:14906-11. 2012
    ..We suggest these structures constitute a self-organizing mechanism that coordinates fiber deposition and the regulation of prion inheritance...
  3. pmc ATP-triggered conformational changes delineate substrate-binding and -folding mechanics of the GroEL chaperonin
    Daniel K Clare
    Crystallography and Institute of Structural and Molecular Biology, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK
    Cell 149:113-23. 2012
    ..The extended conformation also provides a potential docking site for GroES, to trigger the final, 100° domain rotation constituting the "power stroke" that ejects substrate into the folding chamber...
  4. pmc Motor mechanism for protein threading through Hsp104
    Petra Wendler
    Department of Crystallography, Birkbeck College, London, UK
    Mol Cell 34:81-92. 2009
    ..Asymmetric reconstructions of Hsp104 in the presence of ATPgammaS or ATP support sequential rather than concerted ATP hydrolysis in the NBD1 ring...
  5. pmc Topologies of a substrate protein bound to the chaperonin GroEL
    Nadav Elad
    Department of Crystallography, Birkbeck College London, Malet Street, London WC1E 7HX, UK
    Mol Cell 26:415-26. 2007
    ..Finally, substrate binding induced adjustments in GroEL itself, observed mainly as clustering together of apical domains around sites of substrate binding...
  6. pmc Globular tetramers of beta(2)-microglobulin assemble into elaborate amyloid fibrils
    Helen E White
    Department of Crystallography and Institute of Structural and Molecular Biology, Birkbeck College, London, UK
    J Mol Biol 389:48-57. 2009
    ..These findings suggest a more complex superstructure for amyloid than hitherto suspected and prompt a re-evaluation of the defining features of the amyloid fold...
  7. pmc Atypical AAA+ subunit packing creates an expanded cavity for disaggregation by the protein-remodeling factor Hsp104
    Petra Wendler
    Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, UK
    Cell 131:1366-77. 2007
    ..The large cavity could enable the uptake of polypeptide loops without a requirement for exposed N or C termini...
  8. doi request reprint Chaperone machines in action
    Helen R Saibil
    Department of Crystallography and Institute of Structural Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, UK
    Curr Opin Struct Biol 18:35-42. 2008
    ....
  9. ncbi request reprint Molecular chaperones: containers and surfaces for folding, stabilising or unfolding proteins
    H Saibil
    Department of Crystallography, Birkbeck College, London, WC1E 7HX, UK
    Curr Opin Struct Biol 10:251-8. 2000
    ..Structures of chaperone-pilin complexes reveal the mechanism of chaperone interaction in bacterial pilus assembly and there have been major advances in understanding the structure and function of Hsp100 unfoldases...
  10. ncbi request reprint Conformational changes studied by cryo-electron microscopy
    H R Saibil
    Crystallography Department, Birkbeck College, Malet Street, London WC1E 7HX, UK
    Nat Struct Biol 7:711-4. 2000
    ..This review presents a brief summary of the principles and recent advances in macromolecular structure determination by cryo-electron microscopy...
  11. ncbi request reprint Macromolecular structure determination by cryo-electron microscopy
    H R Saibil
    Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, England
    Acta Crystallogr D Biol Crystallogr 56:1215-22. 2000
    ..This review provides a summary of the main advances and a survey of the capabilities of this approach...
  12. pmc Multiple states of a nucleotide-bound group 2 chaperonin
    Daniel K Clare
    Department of Crystallography, Birkbeck College and Institute of Structural Molecular Biology, University of London, London WC1E 7HX, United Kingdom
    Structure 16:528-34. 2008
    ....
  13. ncbi request reprint An expanded protein folding cage in the GroEL-gp31 complex
    Daniel K Clare
    School of Crystallography, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK
    J Mol Biol 358:905-11. 2006
    ..At 56 kDa, gp23 is close to the maximum size limit of a polypeptide that is thought to fit inside the GroEL-GroES folding cage...
  14. pmc The protofilament structure of insulin amyloid fibrils
    Jose L Jimenez
    Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom
    Proc Natl Acad Sci U S A 99:9196-201. 2002
    ..Comparison of the various fibril structures suggests that very small, local changes in beta-sheet twist are important in establishing the long-range coiling of the protofilaments into fibrils of diverse morphology...
  15. doi request reprint Detection and separation of heterogeneity in molecular complexes by statistical analysis of their two-dimensional projections
    Nadav Elad
    School of Crystallography, Birkbeck College and Institute of Structural Molecular Biology, Malet Street, London WC1E 7HX, UK
    J Struct Biol 162:108-20. 2008
    ..A strategy for using this technique was developed on model data sets. Here, we demonstrate the successful application of this method to both model and real EM data on chaperonin-substrate and ribosome-ligand complexes...
  16. ncbi request reprint Dodecameric structure of the small heat shock protein Acr1 from Mycobacterium tuberculosis
    Christopher K Kennaway
    School of Crystallography and Institute of Structural Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX
    J Biol Chem 280:33419-25. 2005
    ..Along with the differential regulation of these two proteins, the differences in their quaternary structures demonstrated here supports their distinct functional roles...
  17. doi request reprint Cryo electron microscopy structures of Hsp100 proteins: crowbars in or out?
    Petra Wendler
    Department of Crystallography, Birkbeck College, Malet St, London WC1E 7HX, UK
    Biochem Cell Biol 88:89-96. 2010
    ..In addition, we present some general assessment criteria for low-resolution cryo-EM maps to offer non-structural biologists tools to evaluate these structures...
  18. doi request reprint Friend or foe: the same fold for attack and defense
    Natalya Lukoyanova
    Department of Crystallography and Institute of Structural Molecular Biology, Birkbeck College, London, UK
    Trends Immunol 29:51-3. 2008
    ....
  19. doi request reprint Structure of a type IV secretion system core complex
    Remi Fronzes
    Institute of Structural and Molecular Biology, School of Crystallography, Birkbeck College, Malet Street, London, WC1E 7HX, UK
    Science 323:266-8. 2009
    ..Overall, the T4SS core complex structure is different in both architecture and composition from the other known double membrane-spanning secretion system that has been structurally characterized...
  20. ncbi request reprint Location of auxilin within a clathrin cage
    Corinne J Smith
    Department of Crystallography, Birkbeck College, Malet Street, WC1E 7HX, England, London, UK
    J Mol Biol 336:461-71. 2004
    ..The means by which auxilin could both aid clathrin coat assembly and displace clathrin from AP2 during uncoating is discussed...
  21. ncbi request reprint The chaperonin folding machine
    Helen R Saibil
    School of Crystallography, Birkbeck College London, Malet Street, London, UK, WC1E 7HX
    Trends Biochem Sci 27:627-32. 2002
  22. ncbi request reprint The mechanism of pore formation by bacterial toxins
    Sarah J Tilley
    School of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, UK
    Curr Opin Struct Biol 16:230-6. 2006
    ..To create the transmembrane pores, these regions must extend and refold into membrane-inserted beta-barrels...
  23. ncbi request reprint Multiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26
    Helen E White
    Department of Crystallography, Birkbeck College, London WC1E 7HX, United Kingdom
    Structure 14:1197-204. 2006
    ..Hinge points between the domains allow a variety of assembly contacts, providing the flexibility required for formation of supercomplexes with non-native proteins...
  24. ncbi request reprint An expanded and flexible form of the vacuolar ATPase membrane sector
    Daniel K Clare
    School of Crystallography, Birkbeck College, University of London, Malet Street, London WC1E 7HX, United Kingdom
    Structure 14:1149-56. 2006
    ..norvegicus. The c-ring was found to be very flexible, most likely as a result of an expanded conformation of the c subunits. This structure may support a role for the vacuolar ATPase c-rings in membrane fusion...
  25. ncbi request reprint Recognition and separation of single particles with size variation by statistical analysis of their images
    Helen E White
    School of Crystallography, Birkbeck College, Malet Street, WC1E 7HX, London, UK
    J Mol Biol 336:453-60. 2004
    ..The strategy developed was used to resolve the structures of two forms of a small heat shock protein (Hsp26) that vary slightly in diameter and subunit packing...
  26. ncbi request reprint Structure determination of macromolecular assemblies by single-particle analysis of cryo-electron micrographs
    Elena V Orlova
    School of Crystallography, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK
    Curr Opin Struct Biol 14:584-90. 2004
    ..Other notable results include the near atomic level resolution structure of the nicotinic acetylcholine receptor in helical arrays and an icosahedral virus structure with an asymmetric polymerase resolved...
  27. ncbi request reprint Structural basis of pore formation by the bacterial toxin pneumolysin
    Sarah J Tilley
    School of Crystallography and Institute of Structural Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom
    Cell 121:247-56. 2005
    ..With an oligomer size of up to 44 subunits in the pore, this assembly creates a transmembrane channel 260 A in diameter lined by 176 beta strands...
  28. doi request reprint Structural basis for the regulated protease and chaperone function of DegP
    Tobias Krojer
    Research Institute for Molecular Pathology IMP, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Nature 453:885-90. 2008
    ..Oligomer reassembly and concomitant activation on substrate binding may also be critical in regulating other HtrA proteases implicated in protein-folding diseases...
  29. ncbi request reprint A mutant chaperonin with rearranged inter-ring electrostatic contacts and temperature-sensitive dissociation
    B Trevor Sewell
    Electron Microscope Unit and Department of Chemistry, University of Cape Town, Rondebosch, South Africa
    Nat Struct Mol Biol 11:1128-33. 2004
    ..Lacking the allosteric signal from the opposite ring, these complexes cannot release their GroES and become trapped, dead-end states...
  30. pmc Allosteric signaling of ATP hydrolysis in GroEL-GroES complexes
    Neil A Ranson
    Astbury Centre for Structural Molecular Biology and Institute of Molecular and Cellular Biology, University of Leeds, Leeds, LS2 9JT, UK
    Nat Struct Mol Biol 13:147-52. 2006
    ....
  31. ncbi request reprint Elongated oligomers assemble into mammalian PrP amyloid fibrils
    M Howard Tattum
    MRC Prion Unit and Department of Neurodegenerative Disease, Institute of Neurology, University College London, Queen Square, London WC1N 3BG, UK
    J Mol Biol 357:975-85. 2006
    ....
  32. pmc Folding with and without encapsulation by cis- and trans-only GroEL-GroES complexes
    George W Farr
    Howard Hughes Medical Institute and Department of Genetics, Yale School of Medicine, Boyer Center, 295 Congress Avenue, New Haven, CT 06510, USA
    EMBO J 22:3220-30. 2003
    ..We conclude that a trans mechanism, involving rounds of binding to an open ring and direct release into the bulk solution, can be generally productive although, where size permits, cis encapsulation supports more efficient folding...