Adolfo Saiardi

Summary

Affiliation: University College London
Country: UK

Publications

  1. doi request reprint The new world of inorganic polyphosphates
    Cristina Azevedo
    Medical Research Council Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, U K
    Biochem Soc Trans 44:13-7. 2016
  2. pmc Analysis of Dictyostelium discoideum inositol pyrophosphate metabolism by gel electrophoresis
    Francesca Pisani
    Medical Research Council Laboratory for Molecular Cell Biology, University College London, London, United Kingdom Department of Biology, Ecology and Earth Science, University of Calabria, Rende, Italy
    PLoS ONE 9:e85533. 2014
  3. doi request reprint How inositol pyrophosphates control cellular phosphate homeostasis?
    Adolfo Saiardi
    Cell Biology Unit, Medical Research Council Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Adv Biol Regul 52:351-9. 2012
  4. doi request reprint Cell signalling by inositol pyrophosphates
    Adolfo Saiardi
    MRC LMCB, Department of Cell and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, UK
    Subcell Biochem 59:413-43. 2012
  5. pmc IP6K gene identification in plant genomes by tag searching
    Fabio Fassetti
    DEIS, Universita della Calabria, Via Pietro Bucci 41C Rende CS Italy
    BMC Proc 5:S1. 2011
  6. pmc Inositol pyrophosphates regulate JMJD2C-dependent histone demethylation
    Adam Burton
    Medical Research Council Cell Biology Unit and Department of Cell and Developmental Biology, Medical Research Council Laboratory for Molecular Cell Biology, and Department of Neuroscience, Physiology, and Pharmacology, University College London, London WC1E 6BT, United Kingdom
    Proc Natl Acad Sci U S A 110:18970-5. 2013
  7. doi request reprint Influence of inositol pyrophosphates on cellular energy dynamics
    Zsolt Szijgyarto
    Cell Biology Unit, Medical Research Council Laboratory for Molecular Cell Biology, and Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Science 334:802-5. 2011
  8. doi request reprint Synthesis of InsP7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1)
    Cristina Azevedo
    Medical Research Council Cell Biology Unit and Laboratory for Molecular Cell Biology, Department of Cell Biology, University College London, London, UK
    Methods Mol Biol 645:73-85. 2010
  9. pmc Inositol pyrophosphate mediated pyrophosphorylation of AP3B1 regulates HIV-1 Gag release
    Cristina Azevedo
    Cell Biology Unit, Medical Research Council Laboratory for Molecular Cell Biology, and Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    Proc Natl Acad Sci U S A 106:21161-6. 2009
  10. pmc Identification of an evolutionarily conserved family of inorganic polyphosphate endopolyphosphatases
    Annalisa Lonetti
    Medical Research Council Cell Biology Unit, Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 286:31966-74. 2011

Collaborators

  • Cristina Azevedo
  • Antonella Riccio
  • Daniel Bosch
  • Shamshad Cockcroft
  • Stephen J Nurrish
  • Assegid Garedew
  • Miranda S C Wilson
  • Adam Burton
  • Zsolt Szijgyarto
  • Thomas M Livermore
  • Bernadett Kolozsv├íri
  • Francesca Pisani
  • Sara Maria Nancy Onnebo
  • Omar Loss
  • Fabio Fassetti
  • Annalisa Lonetti
  • Oriana Losito
  • Suzumi M Tokuoka
  • Simon J Bulley
  • Robin F Irvine
  • Jonathan Robert Chubb
  • Thomas Livermore
  • Giuseppina Rose
  • Marco Gaspari
  • Federica Parisi
  • Catia Andreassi
  • Chun Ting Wu
  • Ofelia Leone
  • Luigi Palopoli
  • Simona E Rombo
  • Adam Cain Resnick
  • Xiaowen Hu

Detail Information

Publications28

  1. doi request reprint The new world of inorganic polyphosphates
    Cristina Azevedo
    Medical Research Council Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, U K
    Biochem Soc Trans 44:13-7. 2016
    ..Modulation of cellular levels of polyP regulates Top1 activity by modifying its polyphosphorylation status. Here we discuss the significance of the recently identified new role of inorganic polyP. ..
  2. pmc Analysis of Dictyostelium discoideum inositol pyrophosphate metabolism by gel electrophoresis
    Francesca Pisani
    Medical Research Council Laboratory for Molecular Cell Biology, University College London, London, United Kingdom Department of Biology, Ecology and Earth Science, University of Calabria, Rende, Italy
    PLoS ONE 9:e85533. 2014
    ....
  3. doi request reprint How inositol pyrophosphates control cellular phosphate homeostasis?
    Adolfo Saiardi
    Cell Biology Unit, Medical Research Council Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Adv Biol Regul 52:351-9. 2012
    ..In this review I will describe the recent literature on this topic, with in the hope of inspiring further research in this fascinating area of biology...
  4. doi request reprint Cell signalling by inositol pyrophosphates
    Adolfo Saiardi
    MRC LMCB, Department of Cell and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, UK
    Subcell Biochem 59:413-43. 2012
    ..The extraordinary range of cellular function controlled by inositol pyrophosphate underline their great importance...
  5. pmc IP6K gene identification in plant genomes by tag searching
    Fabio Fassetti
    DEIS, Universita della Calabria, Via Pietro Bucci 41C Rende CS Italy
    BMC Proc 5:S1. 2011
    ..The IP6K are evolutionary conserved enzymes identified in several mammalian, fungi and amoebae species. Although IP6K has not yet been identified in plant chromosomes, there are many clues suggesting its presences in vegetal cells...
  6. pmc Inositol pyrophosphates regulate JMJD2C-dependent histone demethylation
    Adam Burton
    Medical Research Council Cell Biology Unit and Department of Cell and Developmental Biology, Medical Research Council Laboratory for Molecular Cell Biology, and Department of Neuroscience, Physiology, and Pharmacology, University College London, London WC1E 6BT, United Kingdom
    Proc Natl Acad Sci U S A 110:18970-5. 2013
    ..Importantly, these effects lead to changes in JMJD2C-target gene transcription. Our findings demonstrate that inositol pyrophosphate signaling influences nuclear functions by regulating histone modifications. ..
  7. doi request reprint Influence of inositol pyrophosphates on cellular energy dynamics
    Zsolt Szijgyarto
    Cell Biology Unit, Medical Research Council Laboratory for Molecular Cell Biology, and Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Science 334:802-5. 2011
    ..Metabolic reprogramming through inositol pyrophosphates is an evolutionary conserved mechanism that is also preserved in mammalian systems...
  8. doi request reprint Synthesis of InsP7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1)
    Cristina Azevedo
    Medical Research Council Cell Biology Unit and Laboratory for Molecular Cell Biology, Department of Cell Biology, University College London, London, UK
    Methods Mol Biol 645:73-85. 2010
    ..With purified IP6K1, we produce cold InsP(7) and 5beta[(32)P] InsP(7) that we subsequently use in vitro experiments to phosphorylate proteins extracts from different species...
  9. pmc Inositol pyrophosphate mediated pyrophosphorylation of AP3B1 regulates HIV-1 Gag release
    Cristina Azevedo
    Cell Biology Unit, Medical Research Council Laboratory for Molecular Cell Biology, and Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    Proc Natl Acad Sci U S A 106:21161-6. 2009
    ..This study identifies a cellular process that is regulated by IP(7)-mediated pyrophosphorylation...
  10. pmc Identification of an evolutionarily conserved family of inorganic polyphosphate endopolyphosphatases
    Annalisa Lonetti
    Medical Research Council Cell Biology Unit, Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 286:31966-74. 2011
    ..In addition, we prove that this is an evolutionarily conserved feature because mammalian Nudix hydrolase family members, the three Ddp1 homologues in human cells (DIPP1, DIPP2, and DIPP3), are also capable of degrading poly-P...
  11. doi request reprint Phosphate, inositol and polyphosphates
    Thomas M Livermore
    Medical Research Council Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, U K
    Biochem Soc Trans 44:253-9. 2016
    ..Here we explore the intimate links between phosphate, inositol phosphates and polyP, speculating on the evolution of these relationships. ..
  12. doi request reprint Inositol pyrophosphates modulate hydrogen peroxide signalling
    Sara Maria Nancy Onnebo
    Department of Cell and Developmental Biology, University College London, UK
    Biochem J 423:109-18. 2009
    ..Furthermore, parallel cysteine mutagenesis studies performed on mammalian IP6K1 are suggestive that the ROS signal might be transduced by the direct modification of this evolutionarily conserved class of enzymes...
  13. doi request reprint Inositol pyrophosphates: between signalling and metabolism
    Miranda S C Wilson
    Medical Research Council MRC Cell Biology Unit and Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Biochem J 452:369-79. 2013
    ..Despite the advances that have been made, many aspects of inositol pyrophosphate biology remain far from clear. By evaluating the literature, the present review hopes to promote further research in this absorbing area of biology...
  14. pmc Arginine transcriptional response does not require inositol phosphate synthesis
    Daniel Bosch
    Cell Biology Unit, Medical Research Council Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 287:38347-55. 2012
    ..Our work indicates that inositol phosphates do not regulate arginine-dependent gene expression...
  15. doi request reprint The signaling role of inositol hexakisphosphate kinases (IP6Ks)
    Cristina Azevedo
    Cell Biology Unit, Medical Research Council Laboratory for Molecular Cell Biology MRC LMCB, University College London, London, UK
    Adv Enzyme Regul 51:74-82. 2011
    ..This will hopefully precipitate the development of new ways to detect this modification, for example through the production of antibodies that specifically recognize pyrophosphorylated serines...
  16. doi request reprint Are inositol pyrophosphates signalling molecules?
    Adam Burton
    Medical Research Council, Cell Biology Unit, Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, London, UK
    J Cell Physiol 220:8-15. 2009
    ....
  17. doi request reprint Human ITPK1: a reversible inositol phosphate kinase/phosphatase that links receptor-dependent phospholipase C to Ca2+-activated chloride channels
    Adolfo Saiardi
    Medical Research Council Cell Biology Unit and Laboratory of Molecular Cell Biology, University College London, London, UK
    Sci Signal 1:pe5. 2008
    ..This phenomenon of "intersubstrate" transfer is found only in the human enzyme, which can use Ins(1,3,4)P3 to promote increased cellular concentrations of Ins(3,4,5,6)P4...
  18. doi request reprint Inositol phosphates induce DAPI fluorescence shift
    Bernadett Kolozsvári
    Medical Research Council Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, U K
    Biochem J 460:377-85. 2014
    ..2 mg/g of dry mass). The newly identified fluorescence properties of the DAPI-IP5 and DAPI-IP6 complexes allow the levels and enzymatic conversion of these two important messengers to be rapidly and reliably monitored...
  19. ncbi request reprint Extraction and analysis of soluble inositol polyphosphates from yeast
    Cristina Azevedo
    Medical Research Council Cell Biology Unit, Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK
    Nat Protoc 1:2416-22. 2006
    ..It can easily be applied to other systems and we have included two adaptations of the protocol, one optimized for mammalian cells and the other for Arabidopsis thaliana...
  20. ncbi request reprint Inositol pyrophosphates get the vip1 treatment
    Sara Maria Nancy Onnebo
    Medical Research Council MRC Cell Biology Unit and Laboratory for Molecular Cell Biology, Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK
    Cell 129:647-9. 2007
    ..They identify yeast Vip1 as a new inositol pyrophosphate synthase and show that the products of Vip1 activity regulate a cyclin/cyclin-dependent kinase complex...
  21. doi request reprint Why always lysine? The ongoing tale of one of the most modified amino acids
    Cristina Azevedo
    Medical Research Council Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK Electronic address
    Adv Biol Regul 60:144-50. 2016
    ....
  22. pmc A novel method for the purification of inositol phosphates from biological samples reveals that no phytate is present in human plasma or urine
    Miranda S C Wilson
    Medical Research Council Laboratory for Molecular Cell Biology, University College London, London, UK
    Open Biol 5:150014. 2015
    ..These observations seriously question reports that InsP6 is present in human biofluids and the advisability of using InsP6 as a dietary supplement. ..
  23. doi request reprint Protein polyphosphorylation of lysine residues by inorganic polyphosphate
    Cristina Azevedo
    Medical Research Council Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK Electronic address
    Mol Cell 58:71-82. 2015
    ..We propose that polyphosphorylation adds an additional layer of regulation to nuclear signaling, where many PASK-containing proteins are known to play important roles. ..
  24. pmc Inositol pyrophosphates and their unique metabolic complexity: analysis by gel electrophoresis
    Oriana Losito
    Department of Cell and Developmental Biology, Medical Research Council Cell Biology Unit and Laboratory for Molecular Cell Biology, University College London, London, United States of America
    PLoS ONE 4:e5580. 2009
    ..Though likely involved in a wide range of cellular functions, the study of inositol pyrophosphates has suffered from a lack of readily available methods for their analysis...
  25. doi request reprint Functions of inorganic polyphosphates in eukaryotic cells: a coat of many colours
    Cristina Azevedo
    Cell Biology Unit, MRC Laboratory for Molecular Cell Biology, and Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, U K
    Biochem Soc Trans 42:98-102. 2014
    ..However, polyP analysis and function in higher eukaryotes has been gaining momentum recently. In the present review, we mainly aim to discuss the proposed intracellular functions of polyP in higher eukaryotes and its detection methods. ..
  26. pmc Modulation of inositol polyphosphate levels regulates neuronal differentiation
    Omar Loss
    Cell Biology Unit and Laboratory for Molecular Cell Biology, Medical Research Council, University College London, London WC1E 6BT, United Kingdom Department of Cell and Developmental Biology, University College London, London WC1E 6BT, United Kingdom Department of Neuroscience, University College London, London WC1E 6BT, United Kingdom
    Mol Biol Cell 24:2981-9. 2013
    ..Therefore maintaining appropriate intracellular levels of inositol polyphosphates is necessary for neuronal survival and differentiation...
  27. pmc The mood stabilizer valproate inhibits both inositol- and diacylglycerol-signaling pathways in Caenorhabditis elegans
    Suzumi M Tokuoka
    MRC Cell Biology Unit, MRC Laboratory for Molecular Cell Biology, Department of Neuroscience, Physiology, and Pharmacology, University College London, London WC1E 6BT, United Kingdom
    Mol Biol Cell 19:2241-50. 2008
    ....