Research Topics
| I SabroeSummaryAffiliation: University of Sheffield Country: UK Publications
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Detail Information
Publications
"Dirty little secrets"--endotoxin contamination of recombinant proteinsSonia J Wakelin
MRC Centre for Inflammation Research, University of Edinburgh, UK
Immunol Lett 106:1-7. 2006..We propose that considerable caution is needed in the investigation of TLR4 agonists, particularly when using proteins produced in a bacterium that also houses the most ideal TLR4 agonist, LPS...- Cooperative molecular and cellular networks regulate Toll-like receptor-dependent inflammatory responsesGavin E Morris
Academic Unit of Respiratory Medicine, Division of Genomic Medicine, University of Sheffield, Sheffield, S10 2JF, UK
FASEB J 20:2153-5. 2006..Thus, inflammatory responses to pathogens are regulated by networks in which patterns of TLR expression and colocalization of tissue cells and leukocytes are critical... 
Roles of neutrophils in the regulation of the extent of human inflammation through delivery of IL-1 and clearance of chemokinesAlexander Basran
2 Department of Infection and Immunity, Faculty of Medicine, Dentistry and Health, University of Sheffield, Beech Hill Rd, Sheffield, S10 2RX, UK
J Leukoc Biol 93:7-19. 2013....- Toll-like receptor (TLR)-based networks regulate neutrophilic inflammation in respiratory diseaseI Sabroe
Academic Unit of Respiratory Medicine, Section of Infection, Inflammation and Immunity, School of Medicine and Biomedical Sciences, Royal Hallamshire Hospital, University of Sheffield, Sheffield S10 2JF, U K
Biochem Soc Trans 35:1492-5. 2007..The present review describes some of these networks and the resulting potential for their targeting in respiratory disease... - Toll-like receptors in health and disease: complex questions remainIan Sabroe
Division of Genomic Medicine, University of Sheffield, Royal Hallamshire Hospital, Sheffield, United Kingdom
J Immunol 171:1630-5. 2003 - Regulation of human neutrophil chemokine receptor expression and function by activation of Toll-like receptors 2 and 4Ian Sabroe
Academic Units of Respiratory Medicine, Section of Functional Genomics, Division of Genomic Medicine, University of Sheffield, Sheffield, UK
Immunology 115:90-8. 2005..Thus, although treatment of purified human neutrophils with TLR2 and TLR4 agonists modifies chemokine receptor expression, remaining receptors remain functionally competent... 
Ex vivo-expanded bone marrow CD34+ derived neutrophils have limited bactericidal abilityEmily Patricia Dick
Academic Unit of Respiratory Medicine, Section of Infection, Inflammation and Immunity, School of Medicine and Biomedical Sciences, Royal Hallamshire Hospital, Sheffield, United Kingdom
Stem Cells 26:2552-63. 2008..Disclosure of potential conflicts of interest is found at the end of this article...- Practical and conceptual models of chronic obstructive pulmonary diseaseIan Sabroe
Academic Unit of Respiratory Medicine, Section of Infection, Inflammation, and Immunity, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield, United Kingdom
Proc Am Thorac Soc 4:606-10. 2007.... - Toll-like receptors: their role in allergy and non-allergic inflammatory diseaseI Sabroe
Section of Functional Genomics, Division of Genomic Medicine, University of Sheffield, Sheffield, UK
Clin Exp Allergy 32:984-9. 2002..The likelihood of adverse consequences of such treatments, though possible, may be minimized by use of conjugated vaccines... - The role of TLR activation in inflammationI Sabroe
Academic Unit of Respiratory Medicine, Section of Infection, Inflammation and Immunity, School of Medicine and Biomedical Sciences, University of Sheffield, UK
J Pathol 214:126-35. 2008.... - Targeting the networks that underpin contiguous immunity in asthma and chronic obstructive pulmonary diseaseIan Sabroe
Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, L Floor, Royal Hallamshire Hospital, Sheffield, UK
Am J Respir Crit Care Med 175:306-11. 2007.... - Identifying and hurdling obstacles to translational researchIan Sabroe
Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, Royal Hallamshire Hospital, Sheffield S10 2JF, UK
Nat Rev Immunol 7:77-82. 2007..The purpose of this Opinion article is to examine the nature of these hurdles and to provide food for thought on the main obstacles that impede this process... - Pathological networking: a new approach to understanding COPDIan Sabroe
Academic Unit of Respiratory Medicine, L Floor, Royal Hallamshire Hospital, Sheffield S10 2JF, UK
Thorax 62:733-8. 2007.... - Selective roles for Toll-like receptor (TLR)2 and TLR4 in the regulation of neutrophil activation and life spanIan Sabroe
Academic Unit of Respiratory Medicine, Section of Functional Genomics, Division of Genomic Medicine, University of Sheffield, Sheffield, United Kingdom
J Immunol 170:5268-75. 2003..TLR4 signaling presents itself as a pharmacological target that may allow therapeutic modulation of neutrophil survival by direct and indirect mechanisms at sites of inflammation... - Toll-like receptors and chronic lung diseaseNazia Chaudhuri
Academic Unit of Respiratory Medicine, Division of Genomic Medicine, University of Sheffield, M Floor, Royal Hallamshire Hospital, Sheffield, UK
Clin Sci (Lond) 109:125-33. 2005..This review will explore TLR history, molecular signalling and the potential roles of TLRs in chronic lung disease... - The role of interleukin-1beta in direct and toll-like receptor 4-mediated neutrophil activation and survivalLynne R Prince
Academic Unit of Respiratory Medicine, Division of Genomic Medicine, University of Sheffield, M Floor, Royal Hallamshire Hospital, Sheffield S10 2JF, United Kingdom
Am J Pathol 165:1819-26. 2004..Thus an important paradigm of neutrophil regulation needs to be viewed in the context of a cellular network in which actions of IL-1beta on neutrophils are indirect and mediated by other cells... - The CC chemokine eotaxin (CCL11) is a partial agonist of CC chemokine receptor 2bR Martinelli
Leukocyte Biology Section, Biomedical Sciences Division, Faculty of Medicine, Sir Alexander Fleming Building, Imperial College of Science, Technology, and Medicine, South Kensington, London SW7 2AZ, United Kingdom
J Biol Chem 276:42957-64. 2001.... - What can we learn from highly purified neutrophils?I Sabroe
Division of Genomic Medicine, University of Sheffield, M Floor, Royal Hallamshire Hospital, Sheffield S10 2JF, UK
Biochem Soc Trans 32:468-9. 2004.... - Translational mini-review series on Toll-like receptors: networks regulated by Toll-like receptors mediate innate and adaptive immunityL C Parker
Academic Unit of Respiratory Medicine, School of Medicine and Biological Sciences, University of Sheffield, UK
Clin Exp Immunol 147:199-207. 2007.... - Chemokines, innate and adaptive immunity, and respiratory diseaseI Sabroe
Division of Genomic Medicine, University of Sheffield, UK
Eur Respir J 19:350-5. 2002..Chemokines are fundamental regulators of leukocyte homeostasis and inflammation, and their antagonism by small molecule chemokine receptor antagonists may be of enormous importance in the future treatment of human respiratory disease... - The expression and roles of Toll-like receptors in the biology of the human neutrophilLisa C Parker
Academic Units of Respiratory, Medicine and Cell Biology, Section of Functional Genomics, Division of Genomic Medicine, University of Sheffield, UK
J Leukoc Biol 77:886-92. 2005.... - Toll-like receptor (TLR)2 and TLR4 agonists regulate CCR expression in human monocytic cellsLisa C Parker
Section of Functional Genomics, Division of Genomic Medicine, University of Sheffield, Sheffield, United Kingdom
J Immunol 172:4977-86. 2004..This work provides further insights into the mechanisms that regulate leukocyte recruitment and trafficking during TLR-induced inflammatory responses... - Basic science of the innate immune system and the lungNazia Chaudhuri
Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, UK
Paediatr Respir Rev 9:236-42. 2008..In chronic disease, dysregulated inflammation maintains these systems in a state of constant activation, potentially resulting in tissue damage and progressive disease... - Prolyl hydroxylase 3 (PHD3) is essential for hypoxic regulation of neutrophilic inflammation in humans and miceSarah R Walmsley
Academic Unit of Respiratory Medicine, The Medical School, University of Sheffield, Sheffield, United Kingdom
J Clin Invest 121:1053-63. 2011..These data support what we believe to be a novel function for PHD3 in regulating neutrophil survival in hypoxia and may enable the development of new therapeutics for inflammatory disease... - Toll-like receptor (TLR)2 and TLR4 in human peripheral blood granulocytes: a critical role for monocytes in leukocyte lipopolysaccharide responsesIan Sabroe
Section of Functional Genomics, Division of Genomic Medicine, University of Sheffield, Sheffield, United Kingdom
J Immunol 168:4701-10. 2002..We conclude that LPS has little role in the regulation of peripheral blood eosinophil and basophil function, and that, even in neutrophils, monocytes orchestrate many previously observed leukocyte LPS response patterns... - Endotoxin tolerance induces selective alterations in neutrophil functionLisa C Parker
Division of Genomic Medicine, University of Sheffield, M Floor, Royal Hallamshire Hospital, Sheffield, S10 2JF, UK
J Leukoc Biol 78:1301-5. 2005..Induction of tolerance was associated with a loss of TLR4 surface expression. Tolerance, therefore, induces a selective reprogramming of neutrophil function, but cells retain a predominantly proinflammatory phenotype... - The rise and rise of Staphylococcus aureus: laughing in the face of granulocytesS Anwar
Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK
Clin Exp Immunol 157:216-24. 2009..Unravelling the biology of these interactions is a challenging task, but one which may yield new strategies to address this, as yet, defiant organism... - Translational mini-review series on immunology of vascular disease: inflammation, infections and Toll-like receptors in cardiovascular diseaseJ R Ward
Cardiovascular Research Unit, The University of Sheffield, Sheffield, UK
Clin Exp Immunol 156:386-94. 2009.... - A central role for monocytes in Toll-like receptor-mediated activation of the vasculatureJon R Ward
Cardiovascular Research Unit, School of Medicine and Biomedical Sciences, The University of Sheffield, Sheffield, UK
Immunology 128:58-68. 2009.... - Agonists of toll-like receptors 2 and 4 activate airway smooth muscle via mononuclear leukocytesGavin E Morris
Academic Unit of Respiratory Medicine, Division of Genomic Medicine, University of Sheffield, M Floor, Royal Hallamshire Hospital, Sheffield, S10 2JF, UK
Am J Respir Crit Care Med 171:814-22. 2005.... - Monocyte and macrophage dysfunction as a cause of HIV-1 induced dysfunction of innate immunityP Collini
Department of Infection and Immunity, University of Sheffield, Sheffield, UK
Curr Mol Med 10:727-40. 2010..Future research should focus on refining experimental models and on elucidating the physiological mechanisms of monocyte/ Mϕ dysfunction during HIV 1 infection... - Pathways regulating lipopolysaccharide-induced neutrophil survival revealed by lentiviral transduction of primary human neutrophilsEmily P Dick
University of Sheffield, UK
Immunology 127:249-55. 2009.... - Potentiation of TLR4 signalling by plasmin activityJon R Ward
Division of Genomic Medicine, University of Sheffield Medical School, University of Sheffield, Sheffield S10 2JF, UK
Biochem Biophys Res Commun 341:299-303. 2006..These studies link fibrinolysis and TLR signalling, identifying further mechanisms potentially involved in activation of innate immunity... - The role of Toll-like receptors in the regulation of neutrophil migration, activation, and apoptosisIan Sabroe
Division of Genomic Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield, UK
Clin Infect Dis 41:S421-6. 2005.... - The generation of highly purified primary human neutrophils and assessment of apoptosis in response to Toll-like receptor ligandsLisa C Parker
School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield S10 2JF, UK
Methods Mol Biol 517:191-204. 2009..A variety of methods are available to study neutrophil function: purification of human neutrophils and analysis of their lifespan are described here... - Reducing the toll of inflammatory lung diseaseNazia Chaudhuri
Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, L Floor, Royal Hallamshire Hospital, Sheffield, UK
Chest 131:1550-6. 2007..Our growing knowledge in this field will have a significant impact on the understanding of the pathogenesis of inflammatory diseases, and TLR-based therapies are already in early clinical trials to modify atopic disease severity... - A phosphatidylserine species inhibits a range of TLR- but not IL-1beta-induced inflammatory responses by disruption of membrane microdomainsLisa C Parker
Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield, United Kingdom
J Immunol 181:5606-17. 2008.... - Differential and cell-type specific regulation of responses to Toll-like receptor agonists by ISO-1Peter W West
Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield, UK
Immunology 125:101-10. 2008..ISO-1 is a promising parent molecule which inhibits TLR-induced ERK activation and inflammatory cytokine production in monocytes, whose role may be complicated by cell-type specificity... - A Bayesian approach to copy-number-polymorphism analysis in nuclear pedigreesKonstantina Kosta
School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield, UK
Am J Hum Genet 81:808-12. 2007..We have implemented the basic model for use with nuclear families, and we illustrate its application through an analysis of the CNP located in gene CCL3L1 in 201 families with asthma... - Subversion of a lysosomal pathway regulating neutrophil apoptosis by a major bacterial toxin, pyocyaninLynne R Prince
Academic Unit of Respiratory Medicine, University of Sheffield, Sheffield, UK
J Immunol 180:3502-11. 2008..This may be a pathological pathway of cell death to which neutrophils are particularly susceptible, and could be therapeutically targeted to limit neutrophil death and preserve host responses... - Inhibition of neutrophil apoptosis by ATP is mediated by the P2Y11 receptorKathryn R Vaughan
Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, UK
J Immunol 179:8544-53. 2007..Specific targeting of P2Y11 could retain key immune functions of neutrophils but reduce the injurious effects of increased neutrophil longevity during inflammation... - Impairment of apoptotic cell engulfment by pyocyanin, a toxic metabolite of Pseudomonas aeruginosaStephen M Bianchi
Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield S10 2JF, UK
Am J Respir Crit Care Med 177:35-43. 2008..Whether these metabolites, particularly pyocyanin, can also modulate apoptotic cell engulfment is unknown... - Granulocyte apoptosis in the pathogenesis and resolution of lung diseaseStephen M Bianchi
Academic Unit of Respiratory Medicine, Division of Genomic Medicine, University of Sheffield, M Floor, Royal Hallamshire Hospital, Sheffield S10 2JF, U.K
Clin Sci (Lond) 110:293-304. 2006..This review will explore the physiological roles of granulocyte apoptosis and their importance in infectious and non-infectious lung disease... - Agonists of toll-like receptor (TLR)2 and TLR4 are unable to modulate platelet activation by adenosine diphosphate and platelet activating factorJon R Ward
Section of Functional Genomics, University of Sheffield, UK
Thromb Haemost 94:831-8. 2005.... - Eotaxin (CCL11) and eotaxin-2 (CCL24) induce recruitment of eosinophils, basophils, neutrophils, and macrophages as well as features of early- and late-phase allergic reactions following cutaneous injection in human atopic and nonatopic volunteersAndrew Menzies-Gow
Allergy and Clinical Immunology, National Heart and Lung Institute, Imperial College, London, United Kingdom
J Immunol 169:2712-8. 2002..Thus, intradermal injection of eotaxin and eotaxin-2 in humans induced infiltration of eosinophils and other inflammatory cells as well as changes consistent with CC chemokine-induced mast cell degranulation... - CCR4 blockade does not inhibit allergic airways inflammationDolores M Conroy
Leukocyte Biology Section, Biomedical Sciences Division, Faculty of Medicine, Imperial College, London, United Kingdom
J Leukoc Biol 74:558-63. 2003..This study casts doubts on the validity of CCR4 as a therapeutic target in the treatment of asthma... - CCR4 in human allergen-induced late responses in the skin and lungKayhan T Nouri-Aria
Upper Respiratory Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College of Science, Technology and Medicine, London, GB
Eur J Immunol 32:1933-8. 2002..CCR4 may be important in the recruitment of T lymphocytes at sites of allergic inflammation, in a non-organ-specific manner... - The identification, characterization, and distribution of guinea pig CCR4 and epitope mapping of a blocking antibodyLouise A Jopling
Leukocyte Biology Section, Biomedical Sciences Division, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Exhibition Road, London SW7 2AZ, United Kingdom
J Biol Chem 277:6864-73. 2002..Subsequent analysis of chimeric chemokine receptors indicated that 10E4, a functional inhibitor of gpCCR4 responses, recognized the amino terminus of CCR4... - The role of interleukin-8 and its receptors in inflammatory lung disease: implications for therapyJames E Pease
Leukocyte Biology Section, Biomedical Sciences Division, Faculty of Medicine, Imperial College of Science, Technology and Medicine, South Kensington, London SW7 2AZ, UK
Am J Respir Med 1:19-25. 2002..Clinical trials of such compounds will ultimately provide crucial information currently lacking and will define whether or not IL-8 blockade provides future therapy in pulmonary disease... - Tolerance to microbial TLR ligands: molecular mechanisms and relevance to diseaseAndrei E Medvedev
Department of Microbiology and Immunology, University of Maryland, Baltimore UMB, Baltimore, Maryland 21201, USA
J Endotoxin Res 12:133-50. 2006..In this review, we present current theories of the molecular mechanisms that underlie induction and maintenance of 'microbial tolerance', and discuss the possible relevance of tolerance to several infectious and non-infectious diseases... - The carboxyl terminus of the chemokine receptor CCR3 contains distinct domains which regulate chemotactic signaling and receptor down-regulation in a ligand-dependent mannerIan Sabroe
Leukocyte Biology Section, Biomedical Sciences Division, Imperial College London, UK
Eur J Immunol 35:1301-10. 2005..Thus, the CCR3 C terminus contains distinct domains responsible for the regulation of receptor desensitization and for coupling to chemotactic responses... - Incapacitating the immune system in cystic fibrosisIan Sabroe
Nat Med 13:1417-8. 2007 - Variations in eosinophil chemokine responses: an investigation of CCR1 and CCR3 function, expression in atopy, and identification of a functional CCR1 promoterRhian M Phillips
Leukocyte Biology Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, London, United Kingdom
J Immunol 170:6190-201. 2003..Identification of a functional CCR1 promoter will facilitate the study of possible genetic determinants underlying this potentially important clinical phenotype... - Delta 12-prostaglandin J2, a plasma metabolite of prostaglandin D2, causes eosinophil mobilization from the bone marrow and primes eosinophils for chemotaxisAkos Heinemann
Department of Experimental and Clinical Pharmacology, Karl Franzens University, Graz, Austria
J Immunol 170:4752-8. 2003.... - Responses of leukocytes to chemokines in whole blood and their antagonism by novel CC-chemokine receptor 3 antagonistsShannon A Bryan
National Heart and Lung Institute, and Leukocyte Biology Section, Biomedical Sciences Division, Faculty of Medicine, Imperial College School of Medicine, London, United Kingdom
Am J Respir Crit Care Med 165:1602-9. 2002..Such whole blood methods may also be employed in the investigation of other small molecule chemokine receptor antagonists... - Indomethacin causes prostaglandin D(2)-like and eotaxin-like selective responses in eosinophils and basophilsVictoria E L Stubbs
Leukocyte Biology Section, Biomedical Sciences Division, Imperial College Faculty of Medicine, Imperial College of Science, Technology and Medicine, South Kensington, London SW7 2AZ, United Kingdom
J Biol Chem 277:26012-20. 2002..Indomethacin and its analogues may therefore provide a structural basis from which selective PGD(2) receptor small molecule antagonists may be designed and which may have utility in the treatment of allergic inflammatory disease... - CXCR1+CD4+ T cells in human allergic diseaseJames N Francis
Upper Respiratory Medicine, National Heart and Lung Institute, Imperial College, Dovehouse Street, London SW3 6LY, United Kingdom
J Immunol 172:268-73. 2004..Phenotypic analysis reveals that IFN-gamma expression was lower in the CXCR1+CD4+ cells. The identification of CXCR1 as a marker of allergic rhinitis reveals a possible target for therapeutic intervention in atopic disease...