Affiliation: University of Dundee
Rocha S, Campbell K, Roche K, Perkins N. The p53-inhibitor pifithrin-alpha inhibits firefly luciferase activity in vivo and in vitro. BMC Mol Biol. 2003;4:9 pubmed
..Furthermore, these results demonstrate that when using novel compounds, the choice of vectors used in the experimental procedures might be of great importance for the correct conclusions to be made. ..
Moniz S, Biddlestone J, Rocha S. Growâ‚‚: the HIF system, energy homeostasis and the cell cycle. Histol Histopathol. 2014;29:589-600 pubmed publisher
Biddlestone J, Druker J, Shmakova A, Ferguson G, Swedlow J, Rocha S. Analysis of global RNA synthesis at the single cell level following hypoxia. J Vis Exp. 2014;: pubmed publisher
..This analysis allows the visualization of temporal and cell-to-cell changes in global RNA synthesis following hypoxic stress. ..
D Ignazio L, Rocha S. Hypoxia Induced NF-?B. Cells. 2016;5: pubmed publisher
..In addition, we will discuss the role of NF-?B in the control of the cellular response to hypoxia. ..
Batie M, Druker J, D Ignazio L, Rocha S. KDM2 Family Members are Regulated by HIF-1 in Hypoxia. Cells. 2017;6: pubmed publisher
..Taken together, these results demonstrate that KDM2 is a novel HIF target that can help coordinate the cellular response to hypoxia. In addition, these results might explain why KDM2 levels are often deregulated in human cancers. ..
D Ignazio L, Batie M, Rocha S. Hypoxia and Inflammation in Cancer, Focus on HIF and NF-ÎºB. Biomedicines. 2017;5: pubmed publisher
..We discuss possible new points of therapeutic intervention aiming to harness the current understanding of the HIF-NF-ÎºB crosstalk. ..
Bandarra D, Biddlestone J, Mudie S, MÃ¼ller H, Rocha S. HIF-1Î± restricts NF-ÎºB-dependent gene expression to control innate immunity signals. Dis Model Mech. 2015;8:169-81 pubmed publisher
..These results indicate that HIF-1Î± is required to restrain the NF-ÎºB response, and thus prevents excessive and damaging pro-inflammatory responses. ..
Moniz S, Bandarra D, Biddlestone J, Campbell K, Komander D, Bremm A, et al
. Cezanne regulates E2F1-dependent HIF2Î± expression. J Cell Sci. 2015;128:3082-93 pubmed publisher
..Our results thus suggest that HIF2Î± is controlled transcriptionally in a cell-cycle-dependent manner and in response to oncogenic signalling. ..
Ortmann B, Bensaddek D, Carvalhal S, Moser S, Mudie S, Griffis E, et al
. CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells. J Cell Sci. 2016;129:191-205 pubmed publisher
..These results establish a mechanism by which cell cycle mediators, such as CDKs, temporally control the activity of PHD1, directly altering the regulation of HIF1Î± and Cep192. ..