Daniel John Rigden

..The databases contain hitherto unnoticed close homologues of GPI-PLD from yeast and Dictyostelium discoideum...

..A group of present-day archaebacteria contain a ThrRS obtained from a bacterium by horizontal gene transfer. In some of these cases a vestigial version of the original archaeal ThrRS, of potentially novel function, is maintained...

..Assignment of a PPM fold to SidD offers an important pointer towards identification of further deAMPylases...

..Yet, in these organisms mitochondrial function can often be rapidly remodeled; we contend that in these species turnover of mitochondrial proteins is the product of intraorganellar protease activity...

..Here, distant homology between the structure of Cyt toxins and Erwinia virulence factor (Evf) is demonstrated which, along with sequence conservation analysis, allows a putative lipid binding site to be localised in the toxins...

..As a proof of principle, we have confirmed the novel MIT annotation for USP54 by in vitro profiling of binding to CHMP proteins...

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..These predictions contribute to the function annotation of thousands of proteins...

..The results are consistent with taxon-specific addition of components to a core autophagy machinery during evolution...

..Distant homologies and domain contexts suggest that the N-terminal domain is a novel carbohydrate binding module...

..The crystal structure of the RecBCD prokaryotic DNA repair machinery shows that RecB contains such a nuclease domain at its C-terminus. The RecC C-terminal region was reported as having a novel fold...

..The analysis presented here will inform ongoing efforts toward prediction of similar calcium-binding motifs from sequence information alone...

..The surprising relationship described and explored here between EPP and Sts-1 has implications for our understanding of the function(s) of both...

..Unsuccessful cases are also reported for comparison and the factors influencing the success of this route to structure solution are discussed...

..As with DeltacutA, Deltaedc3 led to reduced rates of transcript degradation. These data link P-bodies to both the optimization and regulation of transcript degradation...

..The analysis presented here has consequences for the prediction of calcium binding from sequence alone...

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..In the present work, we investigate the evolution and expression of aspartic proteases in Strongyloides ratti, which permits a unique comparison between parasitic and free-living adult forms within its life-cycle...

..SUPPLEMENTARY INFORMATION: Supplementary data are available at ftp://ftp.ncbi.nih.gov/pub/galperin/Sporulation.html...

..We propose that 3' modification of adenylated mRNA, which is likely to represent a common eukaryotic process, primes the transcript for decapping and efficient degradation...

..Attention is now turning to means of combining these predictions with information from various other sources...

..Preservation of the bioconjugates can be achieved by a combination of cathepsin inhibition and endosome disruption...

..Conserved residues in the BACON domain are also characteristic of carbohydrate binding while its biased phyletic distribution and other data suggest mucin as a potential specific target...

..Our findings describe a new type of antimicrobial protein with multiple cysteine and histidine residues, and with unique secondary structure...

..The isolated gene will facilitate the search for highly active inhibitors towards boll weevil larvae that may provide a new opportunity to control this important insect pest...