Research Topics
Genomes and Genes | Daniel J RigdenSummaryAffiliation: University of Liverpool Country: UK Publications
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Publications
Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1Jimmy H Saw
Department of Microbiology, University of Hawai i, 2538 The Mall, Honolulu, HI 96822, USA
Genome Biol 9:R161. 2008..The ability of A. flavithermus to grow in super-saturated silica solutions makes it an ideal subject to study the processes of sinter formation, which might be similar to the biomineralization processes that occurred at the dawn of life...- Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis)Gustavo R Oliveira
EMBRAPA Recursos Geneticos e Biotecnologia, PqEB Final W5 Norte Brasília, DF, Brasil
BMC Biotechnol 11:85. 2011..In vitro directed molecular evolution through DNA shuffling and phage display screening was applied to enhance the insecticidal activity of variants of the Cry8Ka1 protein of Bacillus thuringiensis... 
Understanding the cell in terms of structure and function: insights from structural genomicsDaniel J Rigden
School of Biological Sciences, University of Liverpool, Biosciences Building, Crown Street, Liverpool L69 7ZB, UK
Curr Opin Biotechnol 17:457-64. 2006..Attention is now turning to means of combining these predictions with information from various other sources...- Sequence analysis of GerM and SpoVS, uncharacterized bacterial 'sporulation' proteins with widespread phylogenetic distributionDaniel J Rigden
School of Biological Sciences, University of Liverpool, Crown St, Liverpool L69 7ZB, UK
Bioinformatics 24:1793-7. 2008..SUPPLEMENTARY INFORMATION: Supplementary data are available at ftp://ftp.ncbi.nih.gov/pub/galperin/Sporulation.html... - The PA14 domain, a conserved all-beta domain in bacterial toxins, enzymes, adhesins and signaling moleculesDaniel J Rigden
School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK
Trends Biochem Sci 29:335-9. 2004 - The DxDxDG motif for calcium binding: multiple structural contexts and implications for evolutionDaniel J Rigden
School of Biological Sciences, University of Liverpool, Crown St, Liverpool L69 7ZB, UK
J Mol Biol 343:971-84. 2004..The analysis presented here has consequences for the prediction of calcium binding from sequence alone... - An unsuspected ecdysteroid/steroid phosphatase activity in the key T-cell regulator, Sts-1: surprising relationship to insect ecdysteroid phosphate phosphataseLyndsay Davies
School of Biological Sciences, University of Liverpool, Biosciences Building, Liverpool L69 7ZB, United Kingdom
Proteins 67:720-31. 2007..The surprising relationship described and explored here between EPP and Sts-1 has implications for our understanding of the function(s) of both... - Identification of novel aspartic proteases from Strongyloides ratti and characterisation of their evolutionary relationships, stage-specific expression and molecular structureLuciane V Mello
University of Liverpool, UK
BMC Genomics 10:611. 2009..In the present work, we investigate the evolution and expression of aspartic proteases in Strongyloides ratti, which permits a unique comparison between parasitic and free-living adult forms within its life-cycle... - Regulation of ecdysteroid signalling during Drosophila development: identification, characterization and modelling of ecdysone oxidase, an enzyme involved in control of ligand concentrationHajime Takeuchi
Cellular Regulation and Signalling Division, School of Biological Sciences, University of Liverpool, Biosciences Building, Crown Street, Liverpool L69 7ZB, UK
Biochem J 389:637-45. 2005.... 
Ab initio protein modelling reveals novel human MIT domainsDaniel J Rigden
School of Biological Sciences, University of Liverpool, Liverpool, UK
FEBS Lett 583:872-8. 2009..As a proof of principle, we have confirmed the novel MIT annotation for USP54 by in vitro profiling of binding to CHMP proteins...- The histidine phosphatase superfamily: structure and functionDaniel J Rigden
School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK
Biochem J 409:333-48. 2008.... - Does distant homology with Evf reveal a lipid binding site in Bacillus thuringiensis cytolytic toxins?Daniel J Rigden
School of Biological Sciences, University of Liverpool, Crown Street, Liverpool, UK
FEBS Lett 583:1555-60. 2009..Here, distant homology between the structure of Cyt toxins and Erwinia virulence factor (Evf) is demonstrated which, along with sequence conservation analysis, allows a putative lipid binding site to be localised in the toxins... - A new family of bacterial DNA repair proteins annotated by the integration of non-homology, distant homology and structural bioinformatic methodsLuciane V Mello
Structural and Chemical Biology, Institute of Integrative Biology, University of Liverpool, UK School of Life Sciences, Institute of Teaching and Learning, University of Liverpool, UK
FEBS Lett 586:3908-13. 2012..The newly assigned activity is sporadically present in phyla not containing near relatives of the similarly active repair protein AlkB... - Systematic survey of deubiquitinase localization identifies USP21 as a regulator of centrosome- and microtubule-associated functionsSylvie Urbé
Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool L69 3BX, United Kingdom
Mol Biol Cell 23:1095-103. 2012.... - Analysis of glycoside hydrolase family 98: catalytic machinery, mechanism and a novel putative carbohydrate binding moduleDaniel J Rigden
School of Biological Sciences, University of Liverpool, UK
FEBS Lett 579:5466-72. 2005..Distant homologies and domain contexts suggest that the N-terminal domain is a novel carbohydrate binding module... - A distant evolutionary relationship between GPI-specific phospholipase D and bacterial phosphatidylcholine-preferring phospholipase CDaniel J Rigden
School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK
FEBS Lett 569:229-34. 2004..The databases contain hitherto unnoticed close homologues of GPI-PLD from yeast and Dictyostelium discoideum... - Archaea recruited D-Tyr-tRNATyr deacylase for editing in Thr-tRNA synthetaseDaniel J Rigden
School of Biological Sciences, University of Liverpool, Crown St, Liverpool L69 7ZB, United Kingdom
RNA 10:1845-51. 2004..A group of present-day archaebacteria contain a ThrRS obtained from a bacterium by horizontal gene transfer. In some of these cases a vestigial version of the original archaeal ThrRS, of potentially novel function, is maintained... - Structural variability of the ubiquitin specific protease DUSP-UBL double domainsPaul R Elliott
The University of Liverpool, Institute of Integrative Biology, Biosciences Building, Crown Street, Liverpool L69 7ZB, United Kingdom
FEBS Lett 585:3385-90. 2011..We identified structural features that dictate different dispositions between constituent domains, which in turn may influence respective binding properties... - Mining metagenomic data for novel domains: BACON, a new carbohydrate-binding moduleLuciane V Mello
School of Biological Sciences, University of Liverpool, Liverpool, UK
FEBS Lett 584:2421-6. 2010..Conserved residues in the BACON domain are also characteristic of carbohydrate binding while its biased phyletic distribution and other data suggest mucin as a potential specific target... - Cathepsin L digestion of nanobioconjugates upon endocytosisViolaine See
Centre for Cell Imaging, University of Liverpool, UK
ACS Nano 3:2461-8. 2009..Preservation of the bioconjugates can be achieved by a combination of cathepsin inhibition and endosome disruption... - AMPLE: a cluster-and-truncate approach to solve the crystal structures of small proteins using rapidly computed ab initio modelsJaclyn Bibby
Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, England
Acta Crystallogr D Biol Crystallogr 68:1622-31. 2012..It carries out the modelling using locally installed Rosetta, creates search ensembles and automatically performs molecular replacement and model rebuilding... - Autophagy in protists: Examples of secondary loss, lineage-specific innovations, and the conundrum of remodeling a single mitochondrionDaniel J Rigden
School of Biological Sciences, University of Liverpool, Liverpool, UK
Autophagy 5:784-94. 2009..Yet, in these organisms mitochondrial function can often be rapidly remodeled; we contend that in these species turnover of mitochondrial proteins is the product of intraorganellar protease activity... - Molecular replacement using ab initio polyalanine models generated with ROSETTADaniel J Rigden
School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, England
Acta Crystallogr D Biol Crystallogr 64:1288-91. 2008..Unsuccessful cases are also reported for comparison and the factors influencing the success of this route to structure solution are discussed... - Family-wide characterization of the DENN domain Rab GDP-GTP exchange factorsShin ichiro Yoshimura
Cancer Research Centre and 2 Institute of Integrative Biology, University of Liverpool, Liverpool L3 9TA, England, UK
J Cell Biol 191:367-81. 2010..DENND1C, DENND3, DENND5A/5B, MTMR5/13, and MADD activate Rab13, Rab12, Rab39, Rab28, and Rab27A/27B, respectively. Together, these findings provide a basis for future studies on Rab regulation and function... - Genomic determinants of sporulation in Bacilli and Clostridia: towards the minimal set of sporulation-specific genesMichael Y Galperin
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA Institute of Integrative Biology, University of Liverpool, Crown St, Liverpool L69 7ZB, UK
Environ Microbiol 14:2870-90. 2012..These profiles should help further experimental studies of uncharacterized widespread sporulation genes, which would ultimately allow delineation of the minimal set(s) of sporulation-specific genes in Bacilli and Clostridia... - A new type of antimicrobial protein with multiple histidines from the hard tick, Amblyomma hebraeumRen Lai
Cellular Regulation and Signaling Division, School of Biological Sciences, University of Liverpool, Liverpool, UK
FASEB J 18:1447-9. 2004..Our findings describe a new type of antimicrobial protein with multiple cysteine and histidine residues, and with unique secondary structure... - Identification and modelling of a PPM protein phosphatase fold in the Legionella pneumophila deAMPylase SidDDaniel J Rigden
University of Liverpool, Institute of Integrative Biology, Liverpool, UK
FEBS Lett 585:2749-54. 2011..Assignment of a PPM fold to SidD offers an important pointer towards identification of further deAMPylases... - Nucleotide sequence and phylogenetic analyses of the DNA polymerase gene of Anticarsia gemmatalis nucleopolyhedrovirusCaren Cristina Dalmolin
, , W5 Norte Final, , Brazil
Virus Res 110:99-109. 2005.... - 6-Phosphofructo-2-kinase and fructose-2,6-bisphosphatase in Trypanosomatidae. Molecular characterization, database searches, modelling studies and evolutionary analysisNathalie Chevalier
Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry, , Brussels, Belgium
FEBS J 272:3542-60. 2005..A possible explanation for the evolution of multiple monofunctional enzymes and for the presence of the ankyrin-motif repeats in the PFK-2 isoenzymes is presented... - Design of new benzoxazole-2-thione-derived inhibitors of Streptococcus pneumoniae hyaluronan lyase: structure of a complex with a 2-phenylindoleDaniel J Rigden
Institute of Pharmacy, University of Regensburg, 93040 Regensburg, Germany
Glycobiology 16:757-65. 2006..These benzoxazole-2-thiones constitute a new class of inhibitors of bacterial Hyals and are well suited for further optimization of their selectivity, potency, and pharmacokinetic properties... - Genome-based identification and characterization of a putative mucin-binding protein from the surface of Streptococcus pneumoniaeDaniela Bumbaca
Center for Immunobiology and Vaccine Development, Children s Hospital Oakland Research Institute, Oakland, California 94609, USA
Proteins 66:547-58. 2007..It appears to be one of the very few unambiguous pneumococcal adhesin molecules known to date. A hypothetical model constructed by ab initio techniques predicts a novel beta-sandwich protein structure... - Order within a mosaic distribution of mitochondrial c-type cytochrome biogenesis systems?James W A Allen
Department of Biochemistry, University of Oxford, UK
FEBS J 275:2385-402. 2008.... - Sequence analysis and characterization of a novel fibronectin-binding repeat domain from the surface of Streptococcus pneumoniaeDaniela Bumbaca
Children's Hospital Oakland Research Institute, Oakland, California 94609, USA
OMICS 8:341-56. 2004..pneumoniae SP0082 protein and its homologs in other streptococci as fibronectin-binding surface adhesins. The SSURE domain is likely to contain a novel protein fold, which was tentatively modeled using ab initio modeling methods... - Structure and mechanism of action of a cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus with broad specificity phosphatase activityDaniel J Rigden
National Centre of Genetic Resources and Biotechnology, Cenargen Embrapa, S A I N Parque Rural, Final W5, Asa Norte, Brasilia, 70770 900, Brazil
J Mol Biol 315:1129-43. 2002..This PhoE structure will therefore serve as a valuable basis for inference of structural and functional characteristics of these proteins... - Fold recognition analysis of glycosyltransferase families: further members of structural superfamiliesOctavio L Franco
Embrapa Genetic Resources and Biotechnology, Cenargen Embrapa, Brasilia DF, Brazil
Glycobiology 13:707-12. 2003..Furthermore, bioinformatics studies indicate the apparent ease with which mechanism-inverting or retaining-may change during evolution... - Structural diversity of calcium-binding proteins in bacteria: single-handed EF-hands?Daniel J Rigden
National Center of Genetic Resources and Biotechnology, Cenargen Embrapa, Brasilia, D F 70770 900, Brazil
Trends Microbiol 11:295-7. 2003 - Structures of phosphate and trivanadate complexes of Bacillus stearothermophilus phosphatase PhoE: structural and functional analysis in the cofactor-dependent phosphoglycerate mutase superfamilyDaniel J Rigden
National Centre of Genetic Resources and Biotechnology, Cenargen Embrapa, SAIN Parque Rural, Final W5, Asa Norte, 70770 900 Brasília, Brazil
J Mol Biol 325:411-20. 2003..Even within this group substrate specificity is clearly variable highlighting the difficulties of computational functional annotation in the cofactor-dependent phosphoglycerate mutase superfamily... - Unexpected catalytic site variation in phosphoprotein phosphatase homologues of cofactor-dependent phosphoglycerate mutaseDaniel J Rigden
Embrapa Genetic Resources and Biotechnology, Cenargen Embrapa, Parque Estação Biológica, Final W3 Norte, 70770 900 Brasília, Brazil
FEBS Lett 536:77-84. 2003..Unexpected variation in catalytic and substrate-binding residues is observed in the models... - An extracellular calcium-binding domain in bacteria with a distant relationship to EF-handsDaniel J Rigden
National Center of Genetic Resources and Biotechnology, Cenargen/Embrapa, Brasilia D.F. 70770-900, Brazil
FEMS Microbiol Lett 221:103-10. 2003..This loop is thus more widespread than hitherto recognized and the evolution of EF-hand-like domains is probably more complex than previously appreciated... - PrfA protein of Bacillus species: prediction and demonstration of endonuclease activity on DNADaniel J Rigden
National Centre of Genetic Resources and Biotechnology, Cenargen Embrapa, Brasilia, Brazil, D F 70770 900
Protein Sci 11:2370-81. 2002..This activity, much lower on linear or relaxed circular double-stranded DNA or on single-stranded DNA, is consistent with a role for this protein in chromosome segregation, DNA recombination, or DNA repair... - The structure of the D49 phospholipase A2 piratoxin III from Bothrops pirajai reveals unprecedented structural displacement of the calcium-binding loop: possiblerelationship to cooperative substrate bindingDaniel J Rigden
EMBRAPA Recursos Geneticos e Biotecnologia, Cenargen Embrapa, Brasilia, DF, Brazil
Acta Crystallogr D Biol Crystallogr 59:255-62. 2003.... - Sequence conservation in the chagasin family suggests a common trend in cysteine proteinase binding by unrelated protein inhibitorsDaniel J Rigden
National Centre of Genetic Resources and Biotechnology, Cenargen Embrapa, S A I N Parque Rural, Final W5 Norte, 70770 900 Brasília, Brazil
Protein Sci 11:1971-7. 2002.... - Structures of Streptococcus pneumoniae hyaluronate lyase in complex with chondroitin and chondroitin sulfate disaccharides. Insights into specificity and mechanism of actionDaniel J Rigden
Children s Hospital Oakland Research Institute, Oakland, California 94609, USA
J Biol Chem 278:50596-606. 2003..The more efficient processive degradation mechanism has come to be preferred for the unsulfated substrate hyaluronan... - The structure-function relationship in the clostripain family of peptidasesNikolaos E Labrou
Laboratory of Enzyme Technology, Department of Agricultural Biotechnology, Agricultural University of Athens, Greece
Eur J Biochem 271:983-92. 2004..Two possible binding sites for the calcium required for auto-activation could be located. Database searches show that clostripain homologues are not confined to bacterial lineages and reveal an intriguing variety of domain architectures... - Genome-based identification of a carbohydrate binding module in Streptococcus pneumoniae hyaluronate lyaseDaniel J Rigden
Embrapa Genetic Resources and Biotechnology, Cenargen Embrapa, Brasilia DF, Brazil
Proteins 52:203-11. 2003..This observation may help the identification and classification of other carbohydrate binding modules... - Inhibition of trypsin by cowpea thionin: characterization, molecular modeling, and dockingFrancislete R Melo
Departamento de Biologia Celular, Universidade de Brasilia, Brasilia DF, Brasil
Proteins 48:311-9. 2002..In the final model, Lys11 occupies the S1 specificity pocket of trypsin as part of a canonical style interaction... - Use of covariance analysis for the prediction of structural domain boundaries from multiple protein sequence alignmentsDaniel J Rigden
Embrapa Genetic Resources and Biotechnology, Cenargen Embrapa, S A I N Parque Rural, Final W5, Asa Norte, 70770 900, Brasilia, Brazil
Protein Eng 15:65-77. 2002..Finally, a prediction is made for geminivirus AL1 protein which is in full agreement with biochemical data, yielding a plausible, novel threading result... - Insights into the catalytic mechanism of cofactor-independent phosphoglycerate mutase from X-ray crystallography, simulated dynamics and molecular modelingDaniel J Rigden
National Centre of Genetic Resources and Biotechnology, Cenargen Embrapa, S A I N Parque Rural, Final W5, Asa Norte, 70770 900 Brasília, Brazil
J Mol Biol 328:909-20. 2003..These studies will facilitate the development of iPGM inhibitors which, due to the demonstrated importance of this enzyme in many bacteria, would be of great potential clinical significance... - Plant-like traits associated with metabolism of Trypanosoma parasitesVeronique Hannaert
Research Unit for Tropical Diseases and Laboratory of Biochemistry, Christian de Duve Institute of Cellular Pathology and Universite catholique de Louvain, B 1200 Brussels, Belgium
Proc Natl Acad Sci U S A 100:1067-71. 2003..Several of the proteins encoded by these genes are now, in the parasites, found inside highly specialized peroxisomes, called glycosomes, absent from all other eukaryotes, including euglenoids... - Beta-helical catalytic domains in glycoside hydrolase families 49, 55 and 87: domain architecture, modelling and assignment of catalytic residuesDaniel J Rigden
Embrapa Genetic Resources and Biotechnology, Cenargen Embrapa, S A I N Parque Rural, Final W5, Asa Norte, 70770 900 Brasília DF, Brazil
FEBS Lett 530:225-32. 2002..Further analysis of domain architecture reveals the association of carbohydrate binding modules with catalytic GH beta-helices, as well as an unexpected pair of beta-helix domains in GH family 55... - Overlapping binding sites for trypsin and papain on a Kunitz-type proteinase inhibitor from Prosopis julifloraOctavio L Franco
EMBRAPA Recursos Geneticos e Biotecnologia, Cenargen, Brasilia, Brazil
Proteins 49:335-41. 2002..The predicted coincidence of trypsin and papain binding sites, once confirmed, would facilitate the search, by phage display for example, for mutants highly active against both proteinases... - Leishmania donovani phosphofructokinase. Gene characterization, biochemical properties and structure-modeling studiesClaudia Lopez
Instituto de Biologia Experimental, Universidad Central de Venezuela, Caracas, Venezuela
Eur J Biochem 269:3978-89. 2002..Modelling studies and site-directed mutagenesis were employed to shed light on the structural basis for the AMP effector specificity and on ATP/PPi specificity among PFKs... - Iterative database searches demonstrate that glycoside hydrolase families 27, 31, 36 and 66 share a common evolutionary origin with family 13Daniel J Rigden
Embrapa Genetic Resources and Biotechnology, Cenargen Embrapa, S A I N Parque Rural, Final W5, Asa Norte, 70770 900, Brasilia, Brazil
FEBS Lett 523:17-22. 2002..The catalytic, nucleophilic residue common to all these families is thereby determined and candidate catalytic proton donors identified within each family... - Analysis of structure and function of putative surface-exposed proteins encoded in the Streptococcus pneumoniae genome: a bioinformatics-based approach to vaccine and drug designDaniel J Rigden
National Center of Genetic Resources and Biotechnology, Cenargen/Embrapa, , Brazil, D.F. 70770-900
Crit Rev Biochem Mol Biol 38:143-68. 2003..The information obtained from the genome analysis will be instrumental in the development of a more effective prophylactic and/or therapeutic agents to prevent and to treat infections due to S. pneumoniae... - Identification and analysis of catalytic TIM barrel domains in seven further glycoside hydrolase familiesDaniel J Rigden
Embrapa Genetic Resources and Biotechnology, Cenargen Embrapa, Estação Parque Biológico, Final W5, Asa Norte, 70770 900, Brasilia DF, Brazil
FEBS Lett 544:103-11. 2003..Only 327 GH sequences still have no structurally identified catalytic domain... - L-Ascorbic acid 6-hexadecanoate, a potent hyaluronidase inhibitor. X-ray structure and molecular modeling of enzyme-inhibitor complexesAlexander Botzki
Institute of Pharmacy, University of Regensburg, Universitatsstrasse 31, 93040 Regensburg, Germany
J Biol Chem 279:45990-7. 2004..The long alkyl chain seemed to interact with an extended, hydrophobic channel formed by mostly conserved amino acids Ala-84, Leu-91, Tyr-93, Tyr-220, and Leu-344 in BTH... - Characterization of the ligandin site of maize glutathione S-transferase IIrine A Axarli
Laboratory of Enzyme Technology, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855-Athens, Greece
Biochem J 382:885-93. 2004..The inactivation of other GST isoenzymes by VBAR was also investigated, and it was concluded that VBAR may have wide applicability as an affinity label for probing structure-function relationships of GST isoenzymes... - Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae,contains a RecQ helicase-like genePeter W Inglis
EMBRAPA Recursos Geneticos e Biotecnologia, Parque Estação Biológica, Final Av W 5 Norte, Brasilia DF, CEP 70770900, C P 02372, Brazil
Mol Genet Genomics 274:79-90. 2005..Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated... - Molecular modeling and inhibitory activity of cowpea cystatin against bean bruchid pestsJuliana M Aguiar
, UENF, Campos dos Goytacazes, RJ, Brazil
Proteins 63:662-70. 2006..The application of this knowledge in plant protein mutation programs aiming at enhancement of plant defenses to pests is discussed... - Alternate structural conformations of Streptococcus pneumoniae hyaluronan lyase: insights into enzyme flexibility and underlying molecular mechanism of actionDaniel J Rigden
Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
J Mol Biol 358:1165-78. 2006..One of these new structures resembles the S.agalactiae enzyme conformation, and provides evidence of a uniform mechanistic/dynamic behavior of this protein across different bacteria... - Use of phage display to select novel cystatins specific for Acanthoscelides obtectus cysteine proteinasesFrancislete R Melo
EMBRAPA/CENARGEN, , Final W3, Asa Norte, , DF, Brazil
Biochim Biophys Acta 1651:146-52. 2003..obtectus cysteine proteinases than the original sequence and could represent good candidate genes for the production of transgenic plants resistant to this insect pest... - Mutagenic definition of a papain-like catalytic triad, sufficiency of the N-terminal domain for single-site core catalytic enzyme acylation, and C-terminal domain for augmentative metal activation of a eukaryotic phytochelatin synthaseNataliya D Romanyuk
Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Plant Physiol 141:858-69. 2006.... - Evolutionary analysis of fructose 2,6-bisphosphate metabolismPaul A M Michels
Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry, Universite Catholique de Louvain, Brussels, Belgium
IUBMB Life 58:133-41. 2006.... - Autophagy and related processes in trypanosomatids: insights from genomic and bioinformatic analysesMurielle Herman
Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry, , Brussels, Belgium
Autophagy 2:107-18. 2006..A bioinformatics analysis of the trypanosomatidal proteins was carried out, highlighting the paucity of information available regarding their structures and enabling prioritization of targets for future structural biology work... - The crystal structure of Trypanosoma brucei enolase: visualisation of the inhibitory metal binding site III and potential as target for selective, irreversible inhibitionMaria Teresa da Silva Giotto
, , , Brazil
J Mol Biol 331:653-65. 2003..Evidence suggests that this is the first direct visualization of the elusive inhibitory metal site, whose existence has hitherto only been inferred from kinetic data... - Acyl carrier protein phosphodiesterase (AcpH) of Escherichia coli is a non-canonical member of the HD phosphatase/phosphodiesterase familyJacob Thomas
Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
Biochemistry 46:129-36. 2007..We conclude that AcpH is a member of the HD protein family despite the lack of the signature histidine residue... 
The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylaseZhong Yu
Division of Molecular Biology and Centre of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
Nucleic Acids Res 35:2107-15. 2007..We propose that JBP1 is a thymidine hydroxylase responsible for the local amplification of J inserted by JBP2, another putative thymidine hydroxylase...- Structural flexibility in Trypanosoma brucei enolase revealed by X-ray crystallography and molecular dynamicsMarcos V de A S Navarro
Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Sao Carlos SP, Brazil
FEBS J 274:5077-89. 2007..One of the new sulfate structures contains a novel metal-binding site IV within the catalytic site cleft... - Kinetic characterization, structure modelling studies and crystallization of Trypanosoma brucei enolaseVeronique Hannaert
Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry, Universite Catholique de Louvain, Brussels, Belgium
Eur J Biochem 270:3205-13. 2003..T. brucei enolase crystals diffracting at 2.3 A resolution were obtained and will permit us to pursue the determination of structure... - Characterization and functional investigation of an Arabidopsis cDNA encoding a homologue to the d-PGMase superfamilyFabienne Bourgis
, , CP 242, Bd du Triomphe, B-1050 Bruxelles, Belgium
J Exp Bot 56:1129-42. 2005..Two overexpressing At-74 lines showed a clear increase in glucose uptake. This paper introduces the At-74 homologue of the d-PGMase superfamily members and supports a possible role of At-74 in carbohydrate metabolism...