P R Rich

Summary

Affiliation: University College London
Country: UK

Publications

  1. ncbi request reprint A copper metallochaperone for photosynthesis and respiration reveals metal-specific targets, interaction with an importer, and alternative sites for copper acquisition
    Stephen Tottey
    Department of Biochemistry and Genetics, Medical School, University of Newcastle, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Biol Chem 277:5490-7. 2002
  2. ncbi request reprint Radicals associated with the catalytic intermediates of bovine cytochrome c oxidase
    Peter R Rich
    The Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, WC1E 6BT, London, UK
    Biochim Biophys Acta 1554:137-46. 2002
  3. ncbi request reprint ATR-FTIR redox difference spectroscopy of Yarrowia lipolytica and bovine complex I
    Douglas Marshall
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, UK
    Biochemistry 45:5458-67. 2006
  4. ncbi request reprint Role of the PEWY glutamate in hydroquinone-quinone oxidation-reduction catalysis in the Qo Site of cytochrome bc1
    Artur Osyczka
    Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Pennsylvania 19104, USA
    Biochemistry 45:10492-503. 2006
  5. doi request reprint A perspective on Peter Mitchell and the chemiosmotic theory
    Peter R Rich
    Glynn Laboratory of Bioenergetics, Institute of Structural and Molecular Biology, University College London, Gower Street, London, UK
    J Bioenerg Biomembr 40:407-10. 2008
  6. pmc Functions of the hydrophilic channels in protonmotive cytochrome c oxidase
    Peter R Rich
    Glynn Laboratory of Bioenergetics, Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK
    J R Soc Interface 10:20130183. 2013
  7. ncbi request reprint The molecular machinery of Keilin's respiratory chain
    P R Rich
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, U K
    Biochem Soc Trans 31:1095-105. 2003
  8. ncbi request reprint Methods to probe protein transitions with ATR infrared spectroscopy
    Peter R Rich
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London, U K
    Mol Biosyst 3:398-407. 2007
  9. ncbi request reprint A comparison of catalytic site intermediates of cytochrome c oxidase and peroxidases
    P R Rich
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, London WC1E 6BT, UK
    Biochemistry (Mosc) 72:1047-55. 2007
  10. doi request reprint Carboxyl group functions in the heme-copper oxidases: information from mid-IR vibrational spectroscopy
    Peter R Rich
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    Biochim Biophys Acta 1777:912-8. 2008

Collaborators

Detail Information

Publications34

  1. ncbi request reprint A copper metallochaperone for photosynthesis and respiration reveals metal-specific targets, interaction with an importer, and alternative sites for copper acquisition
    Stephen Tottey
    Department of Biochemistry and Genetics, Medical School, University of Newcastle, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Biol Chem 277:5490-7. 2002
    ..Conversely, activity in Deltaatx1DeltactaA was less than in either respective single mutant, revealing that Atx1 can function without the major copper importer and consistent with a role in recycling endogenous copper...
  2. ncbi request reprint Radicals associated with the catalytic intermediates of bovine cytochrome c oxidase
    Peter R Rich
    The Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, WC1E 6BT, London, UK
    Biochim Biophys Acta 1554:137-46. 2002
    ..rad;). These protonation changes may mimic a key step of the proton translocation process...
  3. ncbi request reprint ATR-FTIR redox difference spectroscopy of Yarrowia lipolytica and bovine complex I
    Douglas Marshall
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, UK
    Biochemistry 45:5458-67. 2006
    ..This is the first report of IR properties of ubiquinone in complex I, and the data could be used to estimate a stoichiometry of 0.2-0.4 per complex I...
  4. ncbi request reprint Role of the PEWY glutamate in hydroquinone-quinone oxidation-reduction catalysis in the Qo Site of cytochrome bc1
    Artur Osyczka
    Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Pennsylvania 19104, USA
    Biochemistry 45:10492-503. 2006
    ....
  5. doi request reprint A perspective on Peter Mitchell and the chemiosmotic theory
    Peter R Rich
    Glynn Laboratory of Bioenergetics, Institute of Structural and Molecular Biology, University College London, Gower Street, London, UK
    J Bioenerg Biomembr 40:407-10. 2008
    ..I here review some of his views of that time on structures and mechanisms of several key bioenergetic components in relation to the subsequent advances that have been made...
  6. pmc Functions of the hydrophilic channels in protonmotive cytochrome c oxidase
    Peter R Rich
    Glynn Laboratory of Bioenergetics, Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK
    J R Soc Interface 10:20130183. 2013
    ..Functions of the K, D and H channels found in A1-type cytochrome c oxidases are reviewed in relation to these ideas. Possible control of function by dielectric channels and their evolutionary relation to proton channels is explored. ..
  7. ncbi request reprint The molecular machinery of Keilin's respiratory chain
    P R Rich
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, U K
    Biochem Soc Trans 31:1095-105. 2003
    ..These advances, together with emerging evidence of further diverse roles of mitochondria in health and disease, have prompted a new era of interest in mitochondrial function...
  8. ncbi request reprint Methods to probe protein transitions with ATR infrared spectroscopy
    Peter R Rich
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London, U K
    Mol Biosyst 3:398-407. 2007
    ..Combination of such analyses with atomic 3D structural models derived from X-ray and NMR studies can lead to a deeper understanding of molecular mechanisms of enzymatic reactions...
  9. ncbi request reprint A comparison of catalytic site intermediates of cytochrome c oxidase and peroxidases
    P R Rich
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, London WC1E 6BT, UK
    Biochemistry (Mosc) 72:1047-55. 2007
    ..These comparisons can provide insights into the nature of biological oxygen reduction chemistry and guidance for the engineering of biomimetic synthetic materials...
  10. doi request reprint Carboxyl group functions in the heme-copper oxidases: information from mid-IR vibrational spectroscopy
    Peter R Rich
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    Biochim Biophys Acta 1777:912-8. 2008
    ..These data and their interpretations are discussed in relation to current models of the mechanism of proton and electron coupling in the protonmotive CcO superfamily...
  11. ncbi request reprint Attenuated total reflection Fourier transform infrared studies of redox changes in bovine cytochrome c oxidase: resolution of the redox Fourier transform infrared difference spectrum of heme a(3)
    Peter R Rich
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    Biochemistry 41:967-73. 2002
    ..This difference spectrum is compared with available vibrational and Raman data on homologous oxidases and on heme A model compounds...
  12. doi request reprint The mitochondrial respiratory chain
    Peter R Rich
    Glynn Laboratory of Bioenergetics, Department of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK
    Essays Biochem 47:1-23. 2010
    ..Additional respiratory components that are found in mammalian and plant, fungal and algal mitochondria are also reviewed...
  13. ncbi request reprint The sites of interaction of triphenyltetrazolium chloride with mitochondrial respiratory chains
    P R Rich
    Department of Biology, University College London, Gower Street, London, WC1E 6BT, UK
    FEMS Microbiol Lett 202:181-7. 2001
    ..This revision of the mode of interaction of TTC with respiratory chains has implications for the types of respiratory-defective mutants that might be detected by TTC screening...
  14. ncbi request reprint FTIR studies of the CO and cyanide adducts of fully reduced bovine cytochrome c oxidase
    P R Rich
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, U K
    Biochemistry 40:6441-9. 2001
    ..Data are discussed in relation to the structure of the binuclear center and protonatable groups in its vicinity...
  15. ncbi request reprint Protonation reactions in relation to the coupling mechanism of bovine cytochrome c oxidase
    P R Rich
    The Glynn Laboratory of Bioenergetics, Department of Biology, University College of London, UK
    Biochim Biophys Acta 1459:475-80. 2000
    ..5-8.5. This includes the conserved glutamic acid residue E242 whose pK appears to be above 8.5 even in the fully oxidised enzyme. The results are discussed in relation to recent ideas on coupling mechanism...
  16. ncbi request reprint The quinone chemistry of bc complexes
    Peter R Rich
    The Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    Biochim Biophys Acta 1658:165-71. 2004
    ....
  17. ncbi request reprint Effects of mutation of the conserved glutamic acid-286 in subunit I of cytochrome c oxidase from Rhodobacter sphaeroides
    S Jünemann
    Glynn Laboratory of Bioenergetics, Department of Biology, University College, London, U K
    Biochemistry 38:5248-55. 1999
    ..We propose that the glutamate is implicated in several steps in the catalytic cycle, O --> R, P --> F, and, possibly, F --> O. The results are discussed in relation to the "glutamate trap" model for proton translocation...
  18. ncbi request reprint Direct observation of redox-linked histidine protonation changes in the iron-sulfur protein of the cytochrome bc1 complex by ATR-FTIR spectroscopy
    Masayo Iwaki
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, UK
    Biochemistry 44:4230-7. 2005
    ..It is relatively sharp, has a high extinction coefficient, and provides a novel marker band for the detection of imidazolate intermediates in enzymatic mechanisms generally...
  19. ncbi request reprint An IR study of protonation changes associated with heme-heme electron transfer in bovine cytochrome c oxidase
    Masayo Iwaki
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Am Chem Soc 129:2923-9. 2007
    ....
  20. ncbi request reprint ATR-FTIR spectroscopy of the P(M) and F intermediates of bovine and Paracoccus denitrificans cytochrome c oxidase
    Masayo Iwaki
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, UK
    Biochemistry 42:8809-17. 2003
    ..From comparison with available IR data on model compounds, it is suggested that these reflect changes in the covalent tyrosine-histidine ligand to Cu(B). These findings are discussed in relation to the oxidase catalytic cycle...
  21. ncbi request reprint Redox-induced transitions in bovine cytochrome bc1 complex studied by perfusion-induced ATR-FTIR spectroscopy
    Masayo Iwaki
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, UK
    Biochemistry 42:11109-19. 2003
    ..Some tentative assignments of further signals to specific amino acids are made on the basis of known crystal structures...
  22. ncbi request reprint Second-site reversion analysis is not a reliable method to determine distances in membrane proteins: an assessment using mutations in yeast cytochrome c oxidase subunits I and II
    B Meunier
    Department of Biology, University College London, Gower Street, London, WC1E 6BT, UK
    J Mol Biol 283:727-30. 1998
    ..However, the reversions are not randomly located in the structure but reveal regions essential for assembly or functional units...
  23. ncbi request reprint ATR-FTIR spectroscopy and isotope labeling of the PM intermediate of Paracoccus denitrificans cytochrome c oxidase
    Masayo Iwaki
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, UK
    Biochemistry 43:14370-8. 2004
    ....
  24. ncbi request reprint ATR-FTIR difference spectroscopy of the P(M) intermediate of bovine cytochrome c oxidase
    Masayo Iwaki
    The Glynn Laboratory of Bioenergetics, Department of Biology, University College London, UK
    Biochim Biophys Acta 1555:116-21. 2002
    ..Tentative assignments are made based on vibrational data of related proteins and model compounds...
  25. pmc Three redox states of Trypanosoma brucei alternative oxidase identified by infrared spectroscopy and electrochemistry
    Amandine Maréchal
    Glynn Laboratory of Bioenergetics, Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 284:31827-33. 2009
    ..In addition, when both signals 1 and 2 were reduced, introduction of oxygen caused rapid oxidation of both components. These data are interpreted in terms of the possible active site structure and mechanism of oxygen reduction to water...
  26. doi request reprint Time-resolved FTIR study of CO recombination with horseradish peroxidase
    Amandine Maréchal
    Glynn Laboratory of Bioenergetics, Institute of Structural and Molecular Biology, University College London, London, UK
    Biochem Soc Trans 36:1165-8. 2008
    ..Other signals arising from protein and haem in the 1700-1200 cm(-1) range can also be time-resolved with similar kinetics...
  27. ncbi request reprint Direct detection of formate ligation in cytochrome c oxidase by ATR-FTIR spectroscopy
    Masayo Iwaki
    The Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    J Am Chem Soc 126:2386-9. 2004
    ....
  28. ncbi request reprint ATR-FTIR spectroscopy studies of iron-sulfur protein and cytochrome c1 in the Rhodobacter capsulatus cytochrome bc1 complex
    Masayo Iwaki
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    Biochemistry 43:9477-86. 2004
    ....
  29. ncbi request reprint Structural and chemical changes of the P(M) intermediate of paracoccus denitrificans cytochrome c oxidase revealed by IR spectroscopy with labeled tyrosines and histidine
    Masayo Iwaki
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, UK
    Biochemistry 45:10873-85. 2006
    ..This structural change is proposed to mediate the perturbation of the IR band of the protonated Glu278 headgroup...
  30. doi request reprint CO binding and ligand discrimination in human myeloperoxidase
    Emma J Murphy
    Centre for Molecular Medicine, Division of Medicine, University College London, 5 University Street, London WC1E 6JJ, UK
    Biochemistry 49:2150-8. 2010
    ....
  31. ncbi request reprint Ligand, cofactor, and residue vibrations in the catalytic site of endothelial nitric oxide synthase
    W John Ingledew
    School of Biology, University of St Andrews, UK
    Biochemistry 44:4238-46. 2005
    ..The FTIR data can be modeled on the known structure of the catalytic site and indicate the extent of modulation of vibrational modes upon photolysis of the CO compound...
  32. ncbi request reprint Neuronal nitric oxide synthase ligand and protein vibrations at the substrate binding site. A study by FTIR
    W John Ingledew
    School of Biology, University of St Andrews, St Andrews KY16 9JF, UK
    Biochemistry 41:8377-84. 2002
    ..Preliminary assignments of vibrations are made on the basis of frequencies and the effects of hydrogen/deuterium exchange, and in the light of known atomic structures...
  33. ncbi request reprint Assignments of the Pfr-Pr FTIR difference spectrum of cyanobacterial phytochrome Cph1 using 15N and 13C isotopically labeled phycocyanobilin chromophore
    Jasper J van Thor
    Laboratory of Molecular Biophysics, Rex Richards Building, University of Oxford, South Parks Road, Oxford OX1 3QU, U K
    J Phys Chem B 109:20597-604. 2005
    ..These assignments are discussed in light of the 15Z-E photoisomerization model of phototransformation and the associated protein conformational changes...
  34. ncbi request reprint Molecular recognition between protein and nicotinamide dinucleotide in intact, proton-translocating transhydrogenase studied by ATR-FTIR Spectroscopy
    Masayo Iwaki
    School of Biosciences, University of Birmingham, UK
    J Am Chem Soc 128:2621-9. 2006
    ..The data suggest that NADP(H) binding leads to perturbation of a deeply buried part of the polypeptide backbone and to protonation of a carboxylic acid residue...