Genomes and Genes
Johanna S Rees
Affiliation: University of Cambridge
- Method for suppressing non-specific protein interactions observed with affinity resinsJ S Rees
Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
Methods 54:407-12. 2011..We found the effect varied depending on the bait used, most likely due to its endogenous abundance...
- SILAC-iPAC: a quantitative method for distinguishing genuine from non-specific components of protein complexes by parallel affinity captureJohanna S Rees
Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK Cambridge Centre for Proteomics, University of Cambridge, Cambridge CB2 1QR, UK Electronic address
J Proteomics 115:143-56. 2015..The method is simple and quantitative and will be applicable to many problems in cell and molecular biology. We also report the first chicken beadomes. ..
- Peritrophic membrane contribution to Bt Cry delta-endotoxin susceptibility in Lepidoptera and the effect of CalcofluorJohanna S Rees
Department of Biochemistry, Tennis Court Road, University of Cambridge, Cambs CB21GA, UK
J Invertebr Pathol 100:139-46. 2009..This study therefore concludes that Calcofluor is not as suitable as other toxin enhancing agents such as chitinase...
- Enabling technologies for yeast proteome analysisJohanna Rees
Cambridge Centre for Proteomics, Cambridge Systems Biology Centre, University of Cambridge, Cambridge, UK
Methods Mol Biol 759:149-78. 2011..This comprehensive review also describes future approaches that will aid completion in identifying and characterizing the remaining 20% of the undetermined yeast proteome as well as giving new insight into protein dynamics...
- Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap libraryNick Lowe
The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
Development 141:3994-4005. 2014..Our resource substantially increases the number of available protein traps in Drosophila and identifies new markers for cellular organelles and structures. ..
- In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometryJohanna S Rees
Cambridge Centre for Proteomics, University of Cambridge, Cambridge, UK
Mol Cell Proteomics 10:M110.002386. 2011..melanogaster interactome. Additionally we report contaminant proteins that are persistent with affinity purifications irrespective of the tagged bait...