Johanna S Rees

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. doi Method for suppressing non-specific protein interactions observed with affinity resins
    J S Rees
    Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Methods 54:407-12. 2011
  2. doi SILAC-iPAC: A quantitative method for distinguishing genuine from non-specific components of protein complexes by parallel affinity capture
    Johanna S Rees
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK Cambridge Centre for Proteomics, University of Cambridge, Cambridge CB2 1QR, UK Electronic address
    J Proteomics 115:143-56. 2015
  3. ncbi Peritrophic membrane contribution to Bt Cry delta-endotoxin susceptibility in Lepidoptera and the effect of Calcofluor
    Johanna S Rees
    Department of Biochemistry, Tennis Court Road, University of Cambridge, Cambs CB21GA, UK
    J Invertebr Pathol 100:139-46. 2009
  4. doi Enabling technologies for yeast proteome analysis
    Johanna Rees
    Cambridge Centre for Proteomics, Cambridge Systems Biology Centre, University of Cambridge, Cambridge, UK
    Methods Mol Biol 759:149-78. 2011
  5. doi Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library
    Nick Lowe
    The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Development 141:3994-4005. 2014
  6. pmc In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry
    Johanna S Rees
    Cambridge Centre for Proteomics, University of Cambridge, Cambridge, UK
    Mol Cell Proteomics 10:M110.002386. 2011

Collaborators

Detail Information

Publications6

  1. doi Method for suppressing non-specific protein interactions observed with affinity resins
    J S Rees
    Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Methods 54:407-12. 2011
    ..We found the effect varied depending on the bait used, most likely due to its endogenous abundance...
  2. doi SILAC-iPAC: A quantitative method for distinguishing genuine from non-specific components of protein complexes by parallel affinity capture
    Johanna S Rees
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK Cambridge Centre for Proteomics, University of Cambridge, Cambridge CB2 1QR, UK Electronic address
    J Proteomics 115:143-56. 2015
    ..The method is simple and quantitative and will be applicable to many problems in cell and molecular biology. We also report the first chicken beadomes. ..
  3. ncbi Peritrophic membrane contribution to Bt Cry delta-endotoxin susceptibility in Lepidoptera and the effect of Calcofluor
    Johanna S Rees
    Department of Biochemistry, Tennis Court Road, University of Cambridge, Cambs CB21GA, UK
    J Invertebr Pathol 100:139-46. 2009
    ..This study therefore concludes that Calcofluor is not as suitable as other toxin enhancing agents such as chitinase...
  4. doi Enabling technologies for yeast proteome analysis
    Johanna Rees
    Cambridge Centre for Proteomics, Cambridge Systems Biology Centre, University of Cambridge, Cambridge, UK
    Methods Mol Biol 759:149-78. 2011
    ..This comprehensive review also describes future approaches that will aid completion in identifying and characterizing the remaining 20% of the undetermined yeast proteome as well as giving new insight into protein dynamics...
  5. doi Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library
    Nick Lowe
    The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Development 141:3994-4005. 2014
    ..Our resource substantially increases the number of available protein traps in Drosophila and identifies new markers for cellular organelles and structures. ..
  6. pmc In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry
    Johanna S Rees
    Cambridge Centre for Proteomics, University of Cambridge, Cambridge, UK
    Mol Cell Proteomics 10:M110.002386. 2011
    ..melanogaster interactome. Additionally we report contaminant proteins that are persistent with affinity purifications irrespective of the tagged bait...