Alastair W Poole

Summary

Affiliation: University of Bristol
Country: UK

Publications

  1. ncbi request reprint PKC-interacting proteins: from function to pharmacology
    Alastair W Poole
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Trends Pharmacol Sci 25:528-35. 2004
  2. pmc Protein kinase Calpha: disease regulator and therapeutic target
    Olga Konopatskaya
    Department of Physiology and Pharmacology, School of Medical Sciences, University Walk, Bristol BS81TD, UK
    Trends Pharmacol Sci 31:8-14. 2010
  3. pmc RhoG protein regulates platelet granule secretion and thrombus formation in mice
    Robert Goggs
    From the School of Physiology and Pharmacology
    J Biol Chem 288:34217-29. 2013
  4. pmc The small GTPase Rif is dispensable for platelet filopodia generation in mice
    Robert Goggs
    School of Physiology and Pharmacology, University of Bristol, Bristol, United Kingdom
    PLoS ONE 8:e54663. 2013
  5. pmc Genetic analysis of the role of protein kinase Ctheta in platelet function and thrombus formation
    Kellie J Hall
    Department of Physiology and Pharmacology, University of Bristol, Bristol, United Kingdom
    PLoS ONE 3:e3277. 2008
  6. pmc Absence of platelet phenotype in mice lacking the motor protein myosin Va
    Matthew T Harper
    School of Physiology and Pharmacology, University of Bristol, Bristol, United Kingdom
    PLoS ONE 8:e53239. 2013
  7. ncbi request reprint Evidence that the purinergic receptor P2Y12 potentiates platelet shape change by a Rho kinase-dependent mechanism
    Adam R Hardy
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Platelets 16:415-29. 2005
  8. ncbi request reprint Functional interaction of protein kinase Calpha with the tyrosine kinases Syk and Src in human platelets
    Giordano Pula
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 280:7194-205. 2005
  9. doi request reprint Protein kinase C mediates platelet secretion and thrombus formation through protein kinase D2
    Olga Konopatskaya
    School of Physiology and Pharmacology, Bristol Heart Institute, Bristol, United Kingdom
    Blood 118:416-24. 2011
  10. ncbi request reprint PKCdelta regulates collagen-induced platelet aggregation through inhibition of VASP-mediated filopodia formation
    Giordano Pula
    Department of Pharmacology, School of Medical Sciences, University of Bristol, United Kingdom
    Blood 108:4035-44. 2006

Collaborators

Detail Information

Publications38

  1. ncbi request reprint PKC-interacting proteins: from function to pharmacology
    Alastair W Poole
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Trends Pharmacol Sci 25:528-35. 2004
    ..In this article, we review recent work on C-KIPs and the implications for pharmacological and therapeutic development...
  2. pmc Protein kinase Calpha: disease regulator and therapeutic target
    Olga Konopatskaya
    Department of Physiology and Pharmacology, School of Medical Sciences, University Walk, Bristol BS81TD, UK
    Trends Pharmacol Sci 31:8-14. 2010
    ..Selective targeting of PKCalpha therefore has potential therapeutic value in a wide variety of disease states, although will be technically complicated by the ubiquitous expression and multiple functions of the molecule...
  3. pmc RhoG protein regulates platelet granule secretion and thrombus formation in mice
    Robert Goggs
    From the School of Physiology and Pharmacology
    J Biol Chem 288:34217-29. 2013
    ..Interestingly, tail bleeding times were normal in RhoG(-/-) mice, suggesting that the functions of RhoG in platelets are particularly relevant to thrombotic disorders. ..
  4. pmc The small GTPase Rif is dispensable for platelet filopodia generation in mice
    Robert Goggs
    School of Physiology and Pharmacology, University of Bristol, Bristol, United Kingdom
    PLoS ONE 8:e54663. 2013
    ..The small GTPase Rif (RhoF) provides an alternative route to filopodia generation in other cell types and is expressed in both human and mouse platelets...
  5. pmc Genetic analysis of the role of protein kinase Ctheta in platelet function and thrombus formation
    Kellie J Hall
    Department of Physiology and Pharmacology, University of Bristol, Bristol, United Kingdom
    PLoS ONE 3:e3277. 2008
    ....
  6. pmc Absence of platelet phenotype in mice lacking the motor protein myosin Va
    Matthew T Harper
    School of Physiology and Pharmacology, University of Bristol, Bristol, United Kingdom
    PLoS ONE 8:e53239. 2013
    ..Therefore, we hypothesised that myosin Va may regulate granule secretion or formation in platelets...
  7. ncbi request reprint Evidence that the purinergic receptor P2Y12 potentiates platelet shape change by a Rho kinase-dependent mechanism
    Adam R Hardy
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Platelets 16:415-29. 2005
    ....
  8. ncbi request reprint Functional interaction of protein kinase Calpha with the tyrosine kinases Syk and Src in human platelets
    Giordano Pula
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 280:7194-205. 2005
    ..Therefore, there is a complex interplay between PKCalpha, Syk, and Src involving physical interaction, phosphorylation, translocation within the cell, and functional activity regulation...
  9. doi request reprint Protein kinase C mediates platelet secretion and thrombus formation through protein kinase D2
    Olga Konopatskaya
    School of Physiology and Pharmacology, Bristol Heart Institute, Bristol, United Kingdom
    Blood 118:416-24. 2011
    ..Our results show that in the molecular pathway to secretion, PKD2 is a key component of the PKC-mediated pathway to platelet activation and thrombus formation through its selective regulation of dense granule secretion...
  10. ncbi request reprint PKCdelta regulates collagen-induced platelet aggregation through inhibition of VASP-mediated filopodia formation
    Giordano Pula
    Department of Pharmacology, School of Medical Sciences, University of Bristol, United Kingdom
    Blood 108:4035-44. 2006
    ..PKCdelta is therefore a critical negative regulator of filopodia, and hence platelet aggregation, through a functional interaction with the actin organizer VASP...
  11. ncbi request reprint Regulation of SHP-1 tyrosine phosphatase in human platelets by serine phosphorylation at its C terminus
    Matthew L Jones
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 279:40475-83. 2004
    ....
  12. pmc Functional divergence of platelet protein kinase C (PKC) isoforms in thrombus formation on collagen
    Karen Gilio
    Department of Physiology and Pharmacology, School of Medical Sciences, Bristol University, Bristol BS8 1TD, United Kingdom
    J Biol Chem 285:23410-9. 2010
    ....
  13. pmc Protein kinase Ctheta negatively regulates store-independent Ca2+ entry and phosphatidylserine exposure downstream of glycoprotein VI in platelets
    Matthew T Harper
    Department of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, BS8 1TD Bristol, United Kingdom
    J Biol Chem 285:19865-73. 2010
    ..We propose that PKCtheta normally acts to restrict store-independent Ca(2+) entry during GPVI signaling, which results in reduced PS exposure, limiting platelet procoagulant activity during thrombus formation...
  14. doi request reprint Transient receptor potential channels function as a coincidence signal detector mediating phosphatidylserine exposure
    Matthew T Harper
    School of Physiology and Pharmacology, University of Bristol, Bristol BS8 1TD, UK
    Sci Signal 6:ra50. 2013
    ....
  15. ncbi request reprint Dysfunction of the PI3 kinase/Rap1/integrin α(IIb)β(3) pathway underlies ex vivo platelet hypoactivity in essential thrombocythemia
    Samantha F Moore
    School of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, Bristol, United Kingdom
    Blood 121:1209-19. 2013
    ..These results demonstrate that the PI3 kinase/Rap1 pathway is intrinsically impaired in platelets from JAK2V617F-positive ET patients, resulting in diminished thrombin and thrombopoietin-mediated integrin α(IIb)β(3) activation...
  16. doi request reprint Store-operated calcium entry and non-capacitative calcium entry have distinct roles in thrombin-induced calcium signalling in human platelets
    Matthew T Harper
    School of Physiology and Pharmacology, Bristol Heart Institute, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK
    Cell Calcium 50:351-8. 2011
    ..This suggests that the route of Ca2+ entry is an important regulator of thrombin-induced PS exposure in platelets...
  17. pmc Coincident regulation of PKCdelta in human platelets by phosphorylation of Tyr311 and Tyr565 and phospholipase C signalling
    Kellie J Hall
    Department of Pharmacology, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, U K
    Biochem J 406:501-9. 2007
    ..PKCd is therefore regulated in a coincident fashion, PLC-dependent signals recruiting it to the plasma membrane and by phosphorylation on tyrosine residues, potentiating its activity...
  18. pmc Dual regulation of glycogen synthase kinase 3 (GSK3)α/β by protein kinase C (PKC)α and Akt promotes thrombin-mediated integrin αIIbβ3 activation and granule secretion in platelets
    Samantha F Moore
    School of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 288:3918-28. 2013
    ..Together, these results demonstrate that PKCα and Akt modulate platelet function by phosphorylating and inhibiting GSK3α/β, thereby relieving the negative effect of GSK3α/β on thrombin-mediated platelet activation...
  19. ncbi request reprint P2Y1 and P2Y12 receptors for ADP desensitize by distinct kinase-dependent mechanisms
    Adam R Hardy
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, BS8 1TD, United Kingdom
    Blood 105:3552-60. 2005
    ..This study is the first to show that both P2Y(1) and P2Y(12) desensitize in human platelets, and it reveals ways in which their sensitivity to ADP may be differentially and independently altered...
  20. ncbi request reprint Distinct roles for protein kinase C isoforms in regulating platelet purinergic receptor function
    Stuart J Mundell
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
    Mol Pharmacol 70:1132-42. 2006
    ..This study therefore is the first to reveal distinct roles for PKC isoforms in the regulation of platelet P2Y receptor function and trafficking...
  21. pmc Vasodilator-stimulated phosphoprotein (VASP) is phosphorylated on Ser157 by protein kinase C-dependent and -independent mechanisms in thrombin-stimulated human platelets
    James K T Wentworth
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Biochem J 393:555-64. 2006
    ..This implicates Rho kinase and PKC as the major kinases that phosphorylate VASP Ser157 in response to thrombin in platelets...
  22. pmc PKCalpha regulates platelet granule secretion and thrombus formation in mice
    Olga Konopatskaya
    Department of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, Bristol, United Kingdom
    J Clin Invest 119:399-407. 2009
    ..In summary, the effects of PKCalpha ablation on thrombus formation and granule secretion may implicate PKCalpha as a drug target for antithrombotic therapy...
  23. ncbi request reprint Reciprocal cross-talk between P2Y1 and P2Y12 receptors at the level of calcium signaling in human platelets
    Adam R Hardy
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, BS8 1TD, United Kingdom
    Blood 104:1745-52. 2004
    ..It is likely that this interplay between receptors plays an important role in maintaining the delicate balance between platelet activation and inhibition during normal hemostasis...
  24. doi request reprint Protein kinase C alpha enhances sodium-calcium exchange during store-operated calcium entry in mouse platelets
    Matthew T Harper
    School of Physiology and Pharmacology, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, United Kingdom
    Cell Calcium 48:333-40. 2010
    ..These treatments had no further effect in PKCα(-/-) platelets. These data suggest that PKCα enhances the extent of SOCE in mouse platelets by regulating Ca(2+) entry through the Na(+)/Ca(2+) exchanger...
  25. pmc Characterization of a novel focal adhesion kinase inhibitor in human platelets
    Matthew L Jones
    Department of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Biochem Biophys Res Commun 389:198-203. 2009
    ..Our data show that PF-573,228 is a useful tool for analysis of FAK function in cells and reveal that in human platelets FAK may regulate a rise in cell calcium and platelet spreading...
  26. doi request reprint Critical roles for the actin cytoskeleton and cdc42 in regulating platelet integrin alpha2beta1
    Giordano Pula
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, UK
    Platelets 19:199-210. 2008
    ....
  27. ncbi request reprint Physical and functional interaction between protein kinase C delta and Fyn tyrosine kinase in human platelets
    David Crosby
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 278:24533-41. 2003
    ....
  28. pmc Reciprocal feedback regulation of insulin receptor and insulin receptor substrate tyrosine phosphorylation by phosphoinositide 3-kinase in primary adipocytes
    Ingeborg Hers
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Biochem J 368:875-84. 2002
    ....
  29. ncbi request reprint Distinct clathrin-coated pits sort different G protein-coupled receptor cargo
    Stuart J Mundell
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Traffic 7:1420-31. 2006
    ..Our data therefore reveal distinct populations of CCPs that sort GPCR cargo at the plasma membrane using different kinase-dependent mechanisms...
  30. ncbi request reprint A SHPing tale: perspectives on the regulation of SHP-1 and SHP-2 tyrosine phosphatases by the C-terminal tail
    Alastair W Poole
    Department of Pharmacology, University of Bristol, School of Medical Sciences, Bristol BS8 1TD, UK
    Cell Signal 17:1323-32. 2005
    ..Here we review how the differences in the tails of these proteins may regulate their activities and explain some of their functional differences...
  31. ncbi request reprint Interaction of Bruton's tyrosine kinase and protein kinase Ctheta in platelets. Cross-talk between tyrosine and serine/threonine kinases
    David Crosby
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 277:9958-65. 2002
    ..We propose a reciprocal feedback interaction between Btk and PKCtheta; in platelets, in which PKCtheta; positively modulates activity of Btk, which in turn feeds back negatively upon PKCtheta;...
  32. doi request reprint Platelet signaling-a primer
    Robert Goggs
    School of Physiology and Pharmacology, Faculty of Medical and Veterinary Sciences, University of Bristol, UK
    J Vet Emerg Crit Care (San Antonio) 22:5-29. 2012
    ..To review the receptors and signal transduction pathways involved in platelet plug formation and to highlight links between platelets, leukocytes, endothelium, and the coagulation system...
  33. ncbi request reprint GPIb potentiates GPVI-induced responses in human platelets
    Julie Baker
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
    Platelets 15:207-14. 2004
    ..These findings are important in that they reveal a novel role for vWF in platelet activation as a potentiator of platelet activation by collagen...
  34. ncbi request reprint Antibody cross-linking of human platelet P-selectin induces calcium entry by a mechanism dependent upon Fcgamma receptor IIA
    Jean G Sathish
    Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, BS8 1TD, United Kingdom
    Thromb Haemost 92:598-605. 2004
    ..The calcium rise is mediated primarily by induction of a calcium entry mechanism involving the Na(+)-Ca(2+) exchanger operating in reverse mode, since it was blocked by inhibitors of Na(+)-Ca(2+) exchange, bepridil and 5 mM NiCl(2)...
  35. doi request reprint Using zebrafish (Danio rerio) to assess gene function in thrombus formation
    Christopher M Williams
    Department of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, Bristol, UK
    Methods Mol Biol 788:305-19. 2012
    ....
  36. ncbi request reprint Platelet dense-granule secretion: the [3H]-5-HT secretion assay
    David Crosby
    Department of Pharmacology, University of Bristol, UK
    Methods Mol Biol 272:95-6. 2004
  37. doi request reprint PKCα negatively regulates in vitro proplatelet formation and in vivo platelet production in mice
    Christopher M Williams
    School of Physiology and Pharmacology, Bristol Heart Institute, Bristol Platelet Group, Medical Sciences Building, University of Bristol, University Walk, Bristol, BS8 1TD, UK
    Platelets 25:62-8. 2014
    ..In vivo, these observations may culminate in the observed ability of Prkca(-)(/)(-) mice to recover more rapidly from a thrombocytopenic insult...
  38. ncbi request reprint Protein tyrosine phosphatases
    Matthew L Jones
    Department of Pharmacology, School of Medical Sciences, University of Bristol, UK
    Methods Mol Biol 273:169-78. 2004