Research Topics
Genomes and Genes
| Alastair W PooleSummaryAffiliation: University of Bristol Country: UK Publications
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Detail Information
Publications
PKC-interacting proteins: from function to pharmacologyAlastair W Poole
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
Trends Pharmacol Sci 25:528-35. 2004..In this article, we review recent work on C-KIPs and the implications for pharmacological and therapeutic development...- Functional interaction of protein kinase Calpha with the tyrosine kinases Syk and Src in human plateletsGiordano Pula
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
J Biol Chem 280:7194-205. 2005..Therefore, there is a complex interplay between PKCalpha, Syk, and Src involving physical interaction, phosphorylation, translocation within the cell, and functional activity regulation... - Evidence that the purinergic receptor P2Y12 potentiates platelet shape change by a Rho kinase-dependent mechanismAdam R Hardy
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
Platelets 16:415-29. 2005.... - Regulation of SHP-1 tyrosine phosphatase in human platelets by serine phosphorylation at its C terminusMatthew L Jones
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
J Biol Chem 279:40475-83. 2004.... - PKCdelta regulates collagen-induced platelet aggregation through inhibition of VASP-mediated filopodia formationGiordano Pula
Department of Pharmacology, School of Medical Sciences, University of Bristol, United Kingdom
Blood 108:4035-44. 2006..PKCdelta is therefore a critical negative regulator of filopodia, and hence platelet aggregation, through a functional interaction with the actin organizer VASP... - Distinct roles for protein kinase C isoforms in regulating platelet purinergic receptor functionStuart J Mundell
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
Mol Pharmacol 70:1132-42. 2006..This study therefore is the first to reveal distinct roles for PKC isoforms in the regulation of platelet P2Y receptor function and trafficking... 
Vasodilator-stimulated phosphoprotein (VASP) is phosphorylated on Ser157 by protein kinase C-dependent and -independent mechanisms in thrombin-stimulated human plateletsJames K T Wentworth
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
Biochem J 393:555-64. 2006..This implicates Rho kinase and PKC as the major kinases that phosphorylate VASP Ser157 in response to thrombin in platelets...- P2Y1 and P2Y12 receptors for ADP desensitize by distinct kinase-dependent mechanismsAdam R Hardy
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, BS8 1TD, United Kingdom
Blood 105:3552-60. 2005..This study is the first to show that both P2Y(1) and P2Y(12) desensitize in human platelets, and it reveals ways in which their sensitivity to ADP may be differentially and independently altered... - Coincident regulation of PKCdelta in human platelets by phosphorylation of Tyr311 and Tyr565 and phospholipase C signallingKellie J Hall
Department of Pharmacology, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, U K
Biochem J 406:501-9. 2007..PKCd is therefore regulated in a coincident fashion, PLC-dependent signals recruiting it to the plasma membrane and by phosphorylation on tyrosine residues, potentiating its activity... - Functional divergence of platelet protein kinase C (PKC) isoforms in thrombus formation on collagenKaren Gilio
Department of Physiology and Pharmacology, School of Medical Sciences, Bristol University, Bristol BS8 1TD, United Kingdom
J Biol Chem 285:23410-9. 2010.... - Distinct clathrin-coated pits sort different G protein-coupled receptor cargoStuart J Mundell
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
Traffic 7:1420-31. 2006..Our data therefore reveal distinct populations of CCPs that sort GPCR cargo at the plasma membrane using different kinase-dependent mechanisms... - Characterization of a novel focal adhesion kinase inhibitor in human plateletsMatthew L Jones
Department of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
Biochem Biophys Res Commun 389:198-203. 2009..Our data show that PF-573,228 is a useful tool for analysis of FAK function in cells and reveal that in human platelets FAK may regulate a rise in cell calcium and platelet spreading... - A SHPing tale: perspectives on the regulation of SHP-1 and SHP-2 tyrosine phosphatases by the C-terminal tailAlastair W Poole
Department of Pharmacology, University of Bristol, School of Medical Sciences, Bristol BS8 1TD, UK
Cell Signal 17:1323-32. 2005..Here we review how the differences in the tails of these proteins may regulate their activities and explain some of their functional differences... - Physical and functional interaction between protein kinase C delta and Fyn tyrosine kinase in human plateletsDavid Crosby
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
J Biol Chem 278:24533-41. 2003.... 
Critical roles for the actin cytoskeleton and cdc42 in regulating platelet integrin alpha2beta1Giordano Pula
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, UK
Platelets 19:199-210. 2008....- Store-operated calcium entry and non-capacitative calcium entry have distinct roles in thrombin-induced calcium signalling in human plateletsMatthew T Harper
School of Physiology and Pharmacology, Bristol Heart Institute, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK
Cell Calcium 50:351-8. 2011..This suggests that the route of Ca2+ entry is an important regulator of thrombin-induced PS exposure in platelets... - Reciprocal cross-talk between P2Y1 and P2Y12 receptors at the level of calcium signaling in human plateletsAdam R Hardy
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, BS8 1TD, United Kingdom
Blood 104:1745-52. 2004..It is likely that this interplay between receptors plays an important role in maintaining the delicate balance between platelet activation and inhibition during normal hemostasis... - Reciprocal feedback regulation of insulin receptor and insulin receptor substrate tyrosine phosphorylation by phosphoinositide 3-kinase in primary adipocytesIngeborg Hers
Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
Biochem J 368:875-84. 2002.... - Antibody cross-linking of human platelet P-selectin induces calcium entry by a mechanism dependent upon Fcgamma receptor IIAJean G Sathish
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, BS8 1TD, United Kingdom
Thromb Haemost 92:598-605. 2004..The calcium rise is mediated primarily by induction of a calcium entry mechanism involving the Na(+)-Ca(2+) exchanger operating in reverse mode, since it was blocked by inhibitors of Na(+)-Ca(2+) exchange, bepridil and 5 mM NiCl(2)... - GPIb potentiates GPVI-induced responses in human plateletsJulie Baker
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
Platelets 15:207-14. 2004..These findings are important in that they reveal a novel role for vWF in platelet activation as a potentiator of platelet activation by collagen... - Using zebrafish (Danio rerio) to assess gene function in thrombus formationChristopher M Williams
Department of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, Bristol, UK
Methods Mol Biol 788:305-19. 2012.... - Platelet dense-granule secretion: the [3H]-5-HT secretion assayDavid Crosby
Department of Pharmacology, University of Bristol, UK
Methods Mol Biol 272:95-6. 2004 - Interaction of Bruton's tyrosine kinase and protein kinase Ctheta in platelets. Cross-talk between tyrosine and serine/threonine kinasesDavid Crosby
Department of Pharmacology, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
J Biol Chem 277:9958-65. 2002..We propose a reciprocal feedback interaction between Btk and PKCtheta; in platelets, in which PKCtheta; positively modulates activity of Btk, which in turn feeds back negatively upon PKCtheta;... - Protein kinase Calpha: disease regulator and therapeutic targetOlga Konopatskaya
Department of Physiology and Pharmacology, School of Medical Sciences, University Walk, Bristol BS81TD, UK
Trends Pharmacol Sci 31:8-14. 2010..Selective targeting of PKCalpha therefore has potential therapeutic value in a wide variety of disease states, although will be technically complicated by the ubiquitous expression and multiple functions of the molecule... - Protein tyrosine phosphatasesMatthew L Jones
Department of Pharmacology, School of Medical Sciences, University of Bristol, UK
Methods Mol Biol 273:169-78. 2004