Genomes and Genes
Steven M Pollard
Affiliation: University of Cambridge
- Neural stem cells, neurons, and gliaSteven M Pollard
Institute for Stem Research, University of Edinburgh, Edinburgh, United Kingdom
Methods Enzymol 418:151-69. 2006..It also reports on the current status in transfer of these approaches to human ES cells...
- Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiationSteven M Pollard
Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK
Mol Cell Neurosci 38:393-403. 2008..We speculate that the self-renewing NS cell state may be generated in vitro following transcriptional resetting induced by FGF...
- Investigating radial glia in vitroSteven M Pollard
Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
Prog Neurobiol 83:53-67. 2007..We discuss how these approaches, together with an ability to expand radial glia-like neural stem (NS) cell lines, may offer unique opportunities in basic and applied neurobiology...
- Sox2 and Pax6 maintain the proliferative and developmental potential of gliogenic neural stem cells In vitroSandra Gomez-Lopez
Wellcome Trust Centre for Stem Cell Research and Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QR, United Kingdom
Glia 59:1588-99. 2011..Overall these findings indicate that Sox2 and Pax6 are both critical for self-renewal of differentiation-competent radial glia-like NS cells...
- Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screensSteven M Pollard
Wellcome Trust Centre for Stem Cell Research and Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
Cell Stem Cell 4:568-80. 2009..A proof-of-principle live cell imaging-based chemical screen (450 FDA-approved drugs) identifies both differential sensitivities of GNS cells and a common susceptibility to perturbation of serotonin signaling...
- Imaging-based chemical screens using normal and glioma-derived neural stem cellsDavide Danovi
Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
Biochem Soc Trans 38:1067-71. 2010..These studies provide a framework for future higher-throughput screens...
- Interplay between FGF2 and BMP controls the self-renewal, dormancy and differentiation of rat neural stem cellsYirui Sun
Wellcome Trust Centre for Stem Cell Research and Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB21QR, UK
J Cell Sci 124:1867-77. 2011....
- Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assaysLilian Hook
Stem Cell Sciences UK Ltd, Cambridge, UK
Neurochem Int 59:432-44. 2011..Here, we present validation and utility of a human neural lineage-restricted stem cell-based assay platform, including scale-up and automation, genetic engineering and functional characterization of differentiated progeny...
- Adherent neural stem (NS) cells from fetal and adult forebrainSteven M Pollard
Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, Edinburgh EH9 3JQ, UK
Cereb Cortex 16:i112-20. 2006..We also characterize the growth factor requirements for NS cell derivation and self-renewal. We discuss our current understanding of the relationship of NS cell lines to physiological progenitor cells of fetal and adult CNS...
- Niche-independent symmetrical self-renewal of a mammalian tissue stem cellLuciano Conti
Institute for Stem Cell Research, University of Edinburgh, Edinburgh, United Kingdom
PLoS Biol 3:e283. 2005..These homogenous cultures will enable delineation of molecular mechanisms that define a tissue-specific stem cell and allow direct comparison with pluripotent ES cells...
- Zebrafish mutants identify an essential role for laminins in notochord formationMichael J Parsons
Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
Development 129:3137-46. 2002....