Affiliation: University of Cambridge
Brouwer I, Moschetti T, Candelli A, Garcin E, Modesti M, Pellegrini L, et al
. Two distinct conformational states define the interaction of human RAD51-ATP with single-stranded DNA. EMBO J. 2018;37: pubmed publisher
..Together, our findings provide evidence that hRAD51-ATP filaments can exist in two interconvertible conformational states, which might be functionally relevant for DNA homology recognition and strand exchange. ..
Bollschweiler D, Radu L, Pellegrini L. Cryo-electron tomography of SYCP3 fibers under native conditions. Methods Cell Biol. 2018;145:347-371 pubmed publisher
Pellegrini L, Costa A. New Insights into the Mechanism of DNA Duplication by the Eukaryotic Replisome. Trends Biochem Sci. 2016;41:859-871 pubmed publisher
Pellegrini L. Dual Roles of Ctf18-RFC: Loading the Clamp and Angling for the Polymerase. Structure. 2018;26:1-2 pubmed publisher
..In this issue of Structure, Grabarczyk et al. (2018) reveal that a hook-like structure formed by the unique Dcc1 and Ctf8 subunits mediates the interaction of Ctf18-RFC with the leading strand DNA polymerase ɛ. ..
Pellegrini L. The Pol ?-primase complex. Subcell Biochem. 2012;62:157-69 pubmed publisher
Kolinjivadi A, Sannino V, De Antoni A, Zadorozhny K, Kilkenny M, Técher H, et al
. Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments. Mol Cell. 2017;67:867-881.e7 pubmed publisher
..These results indicate that Brca2 and Rad51 prevent formation of abnormal DNA replication intermediates, whose processing by Smarcal1 and Mre11 predisposes to genome instability. ..
Villa F, Simon A, Ortiz Bazan M, Kilkenny M, Wirthensohn D, Wightman M, et al
. Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase. Mol Cell. 2016;63:385-96 pubmed publisher
..Most strikingly, Ctf4-dependent recruitment of CIP-box proteins couples other processes to DNA synthesis, including rDNA copy-number regulation. ..
Pellegrini L. Structural insights into Cdc45 function: was there a nuclease at the heart of the ancestral replisome?. Biophys Chem. 2017;225:10-14 pubmed publisher
..The CMG helicase of the last archaeal/eukaryotic ancestor might have harboured a single-stranded DNA nuclease activity, conserved in some modern archaea. ..
Kilkenny M, Simon A, Mainwaring J, Wirthensohn D, Holzer S, Pellegrini L. The human CTF4-orthologue AND-1 interacts with DNA polymerase ?/primase via its unique C-terminal HMG box. Open Biol. 2017;7: pubmed publisher
..Our findings provide a biochemical basis for the specific interaction between two critical components of the human replisome, and indicate that important principles of replisome architecture have changed significantly in evolution. ..