D J Owen

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. pmc Crystal structure of the amphiphysin-2 SH3 domain and its role in the prevention of dynamin ring formation
    D J Owen
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 17:5273-85. 1998
  2. ncbi request reprint Linking endocytic cargo to clathrin: structural and functional insights into coated vesicle formation
    D J Owen
    Cambridge Institute for Medical Research and University of Cambridge Department of Clinical Biochemistry, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem Soc Trans 32:1-14. 2004
  3. pmc Molecular basis for recognition of dilysine trafficking motifs by COPI
    Lauren P Jackson
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
    Dev Cell 23:1255-62. 2012
  4. pmc Structural basis of the intracellular sorting of the SNARE VAMP7 by the AP3 adaptor complex
    Helen M Kent
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Dev Cell 22:979-88. 2012
  5. pmc A large-scale conformational change couples membrane recruitment to cargo binding in the AP2 clathrin adaptor complex
    Lauren P Jackson
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
    Cell 141:1220-9. 2010
  6. pmc The molecular basis for the endocytosis of small R-SNAREs by the clathrin adaptor CALM
    Sharon E Miller
    Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge CB2 0XY, UK
    Cell 147:1118-31. 2011
  7. pmc Molecular basis for the sorting of the SNARE VAMP7 into endocytic clathrin-coated vesicles by the ArfGAP Hrb
    Paul R Pryor
    Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge CB2 0XY, UK
    Cell 134:817-27. 2008
  8. ncbi request reprint Adaptors for clathrin coats: structure and function
    David J Owen
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Cambridge CB2 2XY, UK
    Annu Rev Cell Dev Biol 20:153-91. 2004
  9. ncbi request reprint Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes
    M G Ford
    Medical Research Council MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Science 291:1051-5. 2001
  10. ncbi request reprint Molecular architecture and functional model of the endocytic AP2 complex
    Brett M Collins
    Cambridge Institute for Medical Research, University of Cambridge, Department of Clinical Biochemistry, Wellcome Trust MRC Building, Hills Road, United Kingdom
    Cell 109:523-35. 2002

Collaborators

Detail Information

Publications33

  1. pmc Crystal structure of the amphiphysin-2 SH3 domain and its role in the prevention of dynamin ring formation
    D J Owen
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 17:5273-85. 1998
    ..Our data suggest that amphiphysin SH3 domains are important regulators of the multimerization cycle of dynamin in endocytosis...
  2. ncbi request reprint Linking endocytic cargo to clathrin: structural and functional insights into coated vesicle formation
    D J Owen
    Cambridge Institute for Medical Research and University of Cambridge Department of Clinical Biochemistry, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem Soc Trans 32:1-14. 2004
    ..The mechanism of CCV formation is discussed in the light of this structural and biochemical data...
  3. pmc Molecular basis for recognition of dilysine trafficking motifs by COPI
    Lauren P Jackson
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
    Dev Cell 23:1255-62. 2012
    ..Flexibility between β'-COP WD-repeat domains and the location of cargo binding have implications for COPI coat assembly...
  4. pmc Structural basis of the intracellular sorting of the SNARE VAMP7 by the AP3 adaptor complex
    Helen M Kent
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Dev Cell 22:979-88. 2012
    ..The mislocalization can be rescued by transfection with wild-type δ-adaptin but not by δ-adaptin containing mutations that abolish VAMP7 binding, despite in all cases intact AP3 being present and LAMP1 trafficking being rescued...
  5. pmc A large-scale conformational change couples membrane recruitment to cargo binding in the AP2 clathrin adaptor complex
    Lauren P Jackson
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
    Cell 141:1220-9. 2010
    ..Using a range of biophysical techniques, we show that the endocytic cargo binding of AP2 is driven by its interaction with PtdIns4,5P(2)-containing membranes...
  6. pmc The molecular basis for the endocytosis of small R-SNAREs by the clathrin adaptor CALM
    Sharon E Miller
    Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge CB2 0XY, UK
    Cell 147:1118-31. 2011
    ..CALM's role in directing the endocytosis of small R-SNAREs may provide insight into the association of CALM/PICALM mutations with growth retardation, cognitive defects, and Alzheimer's disease...
  7. pmc Molecular basis for the sorting of the SNARE VAMP7 into endocytic clathrin-coated vesicles by the ArfGAP Hrb
    Paul R Pryor
    Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge CB2 0XY, UK
    Cell 134:817-27. 2008
    ..These results elucidate the mechanism of retrieval of a postfusion SNARE complex in clathrin-coated vesicles...
  8. ncbi request reprint Adaptors for clathrin coats: structure and function
    David J Owen
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Cambridge CB2 2XY, UK
    Annu Rev Cell Dev Biol 20:153-91. 2004
    ..This review focuses on the structure of clathrin adaptors and how these structures provide functional information on the mechanism of CCV formation...
  9. ncbi request reprint Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes
    M G Ford
    Medical Research Council MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Science 291:1051-5. 2001
    ..This was shown by using purified components and a budding assay on preformed lipid monolayers. In the presence of AP180, clathrin lattices formed on the monolayer. When AP2 was also present, coated pits were formed...
  10. ncbi request reprint Molecular architecture and functional model of the endocytic AP2 complex
    Brett M Collins
    Cambridge Institute for Medical Research, University of Cambridge, Department of Clinical Biochemistry, Wellcome Trust MRC Building, Hills Road, United Kingdom
    Cell 109:523-35. 2002
    ..A model for AP2 recruitment and activation is proposed...
  11. pmc The structure and function of the beta 2-adaptin appendage domain
    D J Owen
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    EMBO J 19:4216-27. 2000
    ..This results in the displacement of the other ligands from the beta 2 appendage. Thus clathrin binding to an AP2-accessory protein complex would cause the controlled release of accessory proteins at sites of vesicle formation...
  12. ncbi request reprint Molecular switches involving the AP-2 beta2 appendage regulate endocytic cargo selection and clathrin coat assembly
    Melissa A Edeling
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom
    Dev Cell 10:329-42. 2006
    ..Another interaction surface on the beta2 appendage sandwich is identified for proteins such as eps15 and clathrin, suggesting a mechanism by which clathrin displaces eps15 to lattice edges during assembly...
  13. ncbi request reprint Phosphatidylinositol-(4,5)-bisphosphate regulates sorting signal recognition by the clathrin-associated adaptor complex AP2
    Stefan Honing
    Institute for Biochemistry II, University of Gottingen, Heinrich Düker Weg 12, 37073 Gottingen, Germany
    Mol Cell 18:519-31. 2005
    ..The binding of AP2 to acidic-dileucine motifs occurs at a different site from Yxxvarphi binding and is not enhanced by mu2Thr156 phosphorylation...
  14. ncbi request reprint Functional dissection of an AP-2 beta2 appendage-binding sequence within the autosomal recessive hypercholesterolemia protein
    Sanjay K Mishra
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 280:19270-80. 2005
    ..We suggested that biased beta subunit engagement by ARH and the only other beta2 appendage selective adaptor, beta-arrestin, promotes efficient incorporation of this mechanistically distinct subset of CLASPs into clathrin-coated buds...
  15. ncbi request reprint A third specificity-determining site in mu 2 adaptin for sequences upstream of Yxx phi sorting motifs
    D J Owen
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Traffic 2:105-10. 2001
    ..This structure suggests that some sequences can function as a 'three pinned plug', in which internalization activity is not critically dependent on any one of the three interacting side chains...
  16. ncbi request reprint A SNARE-adaptor interaction is a new mode of cargo recognition in clathrin-coated vesicles
    Sharon E Miller
    University of Cambridge, CIMR, Wellcome Trust MRC Building, Hills Road, Cambridge, CB2 0XY, UK
    Nature 450:570-4. 2007
    ..We propose that conceptually similar but mechanistically different interactions will direct the post-Golgi trafficking of many SNAREs...
  17. pmc Binding partners for the COOH-terminal appendage domains of the GGAs and gamma-adaptin
    Winnie W Y Lui
    Department of Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, United Kingdom
    Mol Biol Cell 14:2385-98. 2003
    ..By overexpressing wild-type GGA and gamma appendage domains in cells, we can drive p56 and gamma-synergin, respectively, into the cytosol, suggesting a possible mechanism for selectively disrupting the two pathways...
  18. ncbi request reprint Gamma-COP appendage domain - structure and function
    Peter J Watson
    Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
    Traffic 5:79-88. 2004
    ..On the basis of mutations in the yeast homologue of gamma-COP, Sec21p, a second binding site is proposed to exist on the gamma-COP appendage that interacts with the alpha,beta',epsilon COPI subcomplex...
  19. ncbi request reprint Structural insights into clathrin-mediated endocytosis
    D J Owen
    MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, UK
    Curr Opin Cell Biol 12:467-74. 2000
    ..Recent atomic resolution structures determined by X-ray crystallography now enable the molecular basis for the interactions of some components of the endocytic machinery to be understood in detail...
  20. ncbi request reprint The structure of the GGA1-GAT domain reveals the molecular basis for ARF binding and membrane association of GGAs
    Brett M Collins
    Department of Clinical Biochemistry, University of Cambridge, Hills Road, CB2 2XY, Cambridge, United Kingdom
    Dev Cell 4:321-32. 2003
    ....
  21. ncbi request reprint Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII
    Katherine Bowers
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Wellcome Trust MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 281:5094-105. 2006
    ..Our data suggest that mammalian ESCRTII may be redundant, cargo-specific, or not required for protein sorting at the multivesicular body...
  22. ncbi request reprint Endocytosis and vesicle trafficking
    Philip R Evans
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Curr Opin Struct Biol 12:814-21. 2002
    ..Another theme is the recognition of short sequence motifs in structureless regions of other coat components, allowing dynamic multicomponent networks to be established...
  23. ncbi request reprint Structural basis for binding of accessory proteins by the appendage domain of GGAs
    Brett M Collins
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
    Nat Struct Biol 10:607-13. 2003
    ..Isothermal titration calorimetry was used to assess the affinities of different peptides for the GGA and gamma-appendage domains...
  24. ncbi request reprint Two distinct interaction motifs in amphiphysin bind two independent sites on the clathrin terminal domain beta-propeller
    Adriana E Miele
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Struct Mol Biol 11:242-8. 2004
    ..This property, along with the propensity of the N-terminal BAR domain to bind curved membranes, will preferentially localize amphiphysin and its partner, dynamin, to the periphery of invaginated clathrin lattices...
  25. ncbi request reprint Multiple roles for cyclin G-associated kinase in clathrin-mediated sorting events
    Claire X Zhang
    Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    Traffic 6:1103-13. 2005
    ..Only GAK with a functional J domain could rescue the defect, suggesting that GAK is important for clathrin uncoating. Furthermore, we demonstrated that GAK plays a role in the clathrin-dependent trafficking from the trans Golgi network...
  26. ncbi request reprint A structural explanation for the recognition of tyrosine-based endocytotic signals
    D J Owen
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 282:1327-32. 1998
    ..Specificity was conferred by hydrophobic pockets that bind the tyrosine and leucine in the peptide. In the crystal, the protein forms dimers that could increase the strength and specificity of binding to dimeric receptors...
  27. pmc Structural requirements for PACSIN/Syndapin operation during zebrafish embryonic notochord development
    Melissa A Edeling
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 4:e8150. 2009
    ..Our data confirm linkage between directional migration, endocytosis and cell specification during embryonic morphogenesis and highlight a key role for Pacsin3 in this coupling in the notochord...
  28. pmc Functional analysis of AP-2 alpha and mu2 subunits
    Alison M Motley
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom
    Mol Biol Cell 17:5298-308. 2006
    ..Finally, they establish a new model system that can be used both for additional structure-function analyses, and as a way of testing tagged constructs for function in vivo...
  29. ncbi request reprint Gamma-adaptin appendage domain: structure and binding site for Eps15 and gamma-synergin
    Helen M Kent
    MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
    Structure 10:1139-48. 2002
    ..This observation explains the demonstrated brefeldinA (BFA)-sensitive colocalization of Eps15 and AP1 at the Golgi complex...
  30. ncbi request reprint Non-canonical YXXGPhi endocytic motifs: recognition by AP2 and preferential utilization in P2X4 receptors
    Stephen J Royle
    Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
    J Cell Sci 118:3073-80. 2005
    ..These data demonstrate that the family of potential tyrosine-based endocytic signals must be expanded to include motifs with an additional glycine at Y+3 (YXXGPhi)...
  31. ncbi request reprint Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly
    Brett M Collins
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
    Nat Struct Mol Biol 12:594-602. 2005
    ....
  32. ncbi request reprint Structure of Vps26B and mapping of its interaction with the retromer protein complex
    Brett M Collins
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia
    Traffic 9:366-79. 2008
    ....
  33. doi request reprint Vesicle transport: a new player in APP trafficking
    David J Owen
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
    Curr Biol 20:R413-5. 2010
    ..A new study reveals a key role for the AP4 adaptor protein complex in the Golgi-to-endosome trafficking of APP...