Tom Owen-Hughes

Summary

Affiliation: University of Dundee
Country: UK

Publications

  1. pmc Identification of multiple distinct Snf2 subfamilies with conserved structural motifs
    Andrew Flaus
    Division of Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Nucleic Acids Res 34:2887-905. 2006
  2. pmc Mutations to the histone H3 alpha N region selectively alter the outcome of ATP-dependent nucleosome-remodelling reactions
    Joanna Somers
    Wellcome Trust Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nucleic Acids Res 37:2504-13. 2009
  3. pmc Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms
    Helder Ferreira
    Division of Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    J Mol Biol 374:563-79. 2007
  4. pmc Nucleosomes can invade DNA territories occupied by their neighbors
    Maik Engeholm
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, DD1 5EH, UK
    Nat Struct Mol Biol 16:151-8. 2009
  5. pmc The DNA-binding domain of the Chd1 chromatin-remodelling enzyme contains SANT and SLIDE domains
    Daniel P Ryan
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 30:2596-609. 2011
  6. pmc Making sense of transcribing chromatin
    Tom Owen-Hughes
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Curr Opin Cell Biol 24:296-304. 2012
  7. pmc Pyrosequencing positions nucleosomes precisely
    Tom Owen-Hughes
    Division of Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Genome Biol 8:217. 2007
  8. ncbi request reprint Molecular biology. Breaking the silence
    Tom Owen-Hughes
    Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Science 303:324-5. 2004
  9. ncbi request reprint Colworth memorial lecture. Pathways for remodelling chromatin
    T Owen-Hughes
    Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, Department of Biochemistry, University of Dundee, Dundee, DD1 5EH, UK
    Biochem Soc Trans 31:893-905. 2003
  10. ncbi request reprint Snf2-family proteins: chromatin remodellers for any occasion
    Daniel P Ryan
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, DD1 5EH, UK
    Curr Opin Chem Biol 15:649-56. 2011

Collaborators

Detail Information

Publications40

  1. pmc Identification of multiple distinct Snf2 subfamilies with conserved structural motifs
    Andrew Flaus
    Division of Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Nucleic Acids Res 34:2887-905. 2006
    ....
  2. pmc Mutations to the histone H3 alpha N region selectively alter the outcome of ATP-dependent nucleosome-remodelling reactions
    Joanna Somers
    Wellcome Trust Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nucleic Acids Res 37:2504-13. 2009
    ..They also show how alterations to histones can affect the products generated as a result of ATP-dependent remodelling reactions...
  3. pmc Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms
    Helder Ferreira
    Division of Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    J Mol Biol 374:563-79. 2007
    ..These observations illustrate a range of different means by which modifications to histones can influence the action of remodelling enzymes...
  4. pmc Nucleosomes can invade DNA territories occupied by their neighbors
    Maik Engeholm
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, DD1 5EH, UK
    Nat Struct Mol Biol 16:151-8. 2009
    ....
  5. pmc The DNA-binding domain of the Chd1 chromatin-remodelling enzyme contains SANT and SLIDE domains
    Daniel P Ryan
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 30:2596-609. 2011
    ..Through inclusion of Chd1 sequences in homology searches SLIDE domains were identified in CHD6-9 proteins. Point mutations to conserved amino acids within the human CHD7 SLIDE domain have been identified in patients with CHARGE syndrome...
  6. pmc Making sense of transcribing chromatin
    Tom Owen-Hughes
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Curr Opin Cell Biol 24:296-304. 2012
    ..This conundrum is at its most flagrant when considering the need for nucleic acid polymerase's to transit 1000's of based pairs of DNA wrapped as arrays of nucleosomes...
  7. pmc Pyrosequencing positions nucleosomes precisely
    Tom Owen-Hughes
    Division of Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Genome Biol 8:217. 2007
    ..New parallel-sequencing technology has recently been used to map with unprecedented accuracy the positions of nucleosomes enriched for the histone variant H2A.Z throughout the yeast genome...
  8. ncbi request reprint Molecular biology. Breaking the silence
    Tom Owen-Hughes
    Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Science 303:324-5. 2004
  9. ncbi request reprint Colworth memorial lecture. Pathways for remodelling chromatin
    T Owen-Hughes
    Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, Department of Biochemistry, University of Dundee, Dundee, DD1 5EH, UK
    Biochem Soc Trans 31:893-905. 2003
    ..This provides a means by which they could alter both the twist and writhe of DNA on the surface of nucleosomes, and so accelerate nucleosome repositioning...
  10. ncbi request reprint Snf2-family proteins: chromatin remodellers for any occasion
    Daniel P Ryan
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, DD1 5EH, UK
    Curr Opin Chem Biol 15:649-56. 2011
    ..Recent developments include new insights into the mechanism for nucleosome spacing and histone dimer exchange; together with growing evidence for the involvement of Snf2 proteins in DNA repair...
  11. pmc Evidence for DNA translocation by the ISWI chromatin-remodeling enzyme
    Iestyn Whitehouse
    Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Mol Cell Biol 23:1935-45. 2003
    ..In combination, these observations suggest that ISWI is a 3'-5'-strand-specific, ATP-dependent DNA translocase that may be capable of forcing DNA over the surface of nucleosomes...
  12. pmc Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities
    Michael Bruno
    Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Cell 12:1599-606. 2003
    ..This provides eukaryotes with additional means by which they may manipulate chromatin structure...
  13. pmc Analysis of nucleosome repositioning by yeast ISWI and Chd1 chromatin remodeling complexes
    Chris Stockdale
    Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 281:16279-88. 2006
    ..These observations suggest that the accessibility of linker DNA together with the positioning properties of the underlying DNA play important roles in determining the outcome of remodeling by these enzymes...
  14. pmc Structural plasticity of histones H3-H4 facilitates their allosteric exchange between RbAp48 and ASF1
    Wei Zhang
    Department of Biochemistry, University of Cambridge, Cambridge, UK
    Nat Struct Mol Biol 20:29-35. 2013
    ..Exchange of histones H3-H4 between these two histone chaperones has a central role in the assembly of new nucleosomes, and we show here that the H3-H4 complex has an unexpected structural plasticity, which is important for this exchange...
  15. pmc A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization
    Triantaffyllos Gkikopoulos
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    Science 333:1758-60. 2011
    ..These observations indicate that adenosine triphosphate-dependent remodeling enzymes are responsible for directing the positioning of the majority of nucleosomes within the Saccharomyces cerevisiae genome...
  16. pmc The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4
    Triantaffyllos Gkikopoulos
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 30:1919-27. 2011
    ..This suggests a role for Snf2 in the maintenance of point centromeres involving the removal of Cse4 from ectopic sites...
  17. pmc Histone tails and the H3 alphaN helix regulate nucleosome mobility and stability
    Helder Ferreira
    Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, Department of Biochemistry, University of Dundee, Dundee, United Kingdom
    Mol Cell Biol 27:4037-48. 2007
    ..This raises the possibility that modifications to histone proteins may provide a means of fine-tuning specific aspects of the dynamic properties of nucleosomes to the context in which they are located...
  18. pmc The histone chaperones Nap1 and Vps75 bind histones H3 and H4 in a tetrameric conformation
    Andrew Bowman
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD15EH, UK
    Mol Cell 41:398-408. 2011
    ..This alternate mode of histone interaction provides a potential means of maintaining the integrity of the histone tetramer during cycles of nucleosome reassembly...
  19. pmc Sin mutations alter inherent nucleosome mobility
    Andrew Flaus
    Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 23:343-53. 2004
    ..Thus, variation of histone sequence or subtype provides a means by which eukaryotes may regulate access to chromatin through alterations to nucleosome mobility...
  20. ncbi request reprint Site-specific attachment of reporter compounds to recombinant histones
    Michael Bruno
    Division of Gene Regulation and Expression, Wellcome Trust Biocentre, Department of Biochemistry, University of Dundee, Dundee, DD1 5EH, Scotland, United Kingdom
    Methods Enzymol 375:211-28. 2004
  21. pmc EPR distance measurements in deuterated proteins
    Richard Ward
    School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    J Magn Reson 207:164-7. 2010
    ..Longer T(m) gives greatly enhanced sensitivity, much extended distance measurement, more reliable distance distribution calculation and better baseline correction...
  22. ncbi request reprint Mechanisms for ATP-dependent chromatin remodelling: farewell to the tuna-can octamer?
    Andrew Flaus
    Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    Curr Opin Genet Dev 14:165-73. 2004
    ..This provides a new means by which the incorporation of histone variants can be directed. Additional observations support roles for ATP-dependent remodelling enzymes throughout the transcription cycle...
  23. pmc Dynamic properties of nucleosomes during thermal and ATP-driven mobilization
    Andrew Flaus
    Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Cell Biol 23:7767-79. 2003
    ..The dynamic properties of nucleosomes we describe are likely to influence their role in gene regulation...
  24. pmc Probing the (H3-H4)2 histone tetramer structure using pulsed EPR spectroscopy combined with site-directed spin labelling
    Andrew Bowman
    Wellcome Trust Centre for Gene Regulation and Expression, Nucleic Acid Structure Research Group, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nucleic Acids Res 38:695-707. 2010
    ..The approach described here promises to prove a powerful system for investigating the structure of additional assemblies of histones with other important factors in chromatin assembly/fluidity...
  25. ncbi request reprint ATP-dependent chromatin remodeling activities
    K Havas
    Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, University of Dundee, Scotland, United Kingdom
    Cell Mol Life Sci 58:673-82. 2001
    ....
  26. pmc Micrococcal nuclease does not substantially bias nucleosome mapping
    James Allan
    Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK
    J Mol Biol 417:152-64. 2012
    ..These results indicate that biases in nucleosome positioning data collected using MNase are, under our conditions, not significant...
  27. ncbi request reprint The histone chaperones Vps75 and Nap1 form ring-like, tetrameric structures in solution
    Andrew Bowman
    Centre for Gene Regulation and Expression, University of Dundee, Dundee, DD1 5EH, UK
    Nucleic Acids Res 42:6038-51. 2014
    ..The tetramerisation of NAP-1 fold histone chaperones may act to shield acidic surfaces in the absence of histone cargo thus providing a 'self-chaperoning' type mechanism. ..
  28. ncbi request reprint Mechanisms for ATP-dependent chromatin remodelling
    A Flaus
    The Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, DD1 5EH, Dundee, UK
    Curr Opin Genet Dev 11:148-54. 2001
    ....
  29. pmc The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4
    Aleksandra A Watson
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    J Mol Biol 422:3-17. 2012
    ....
  30. ncbi request reprint Sulfyhydryl-reactive site-directed cross-linking as a method for probing the tetrameric structure of histones H3 and H4
    Andrew Bowman
    The Wellcome Trust Biocentre, University of Dundee, Dundee, Scotland, UK
    Methods Mol Biol 833:373-87. 2012
    ..The approaches described may be generally applicable for monitoring the interactions of a diverse range of multi-protein complexes...
  31. ncbi request reprint The FHA domain protein SNIP1 is a regulator of the cell cycle and cyclin D1 expression
    Kevin C Roche
    Division of Gene Regulation and Expression, School of Life Sciences, MSI WTB complex, Dow Street, University of Dundee, Dundee, DD1 5EH, UK
    Oncogene 23:8185-95. 2004
    ..These results define both a new function for SNIP1 and identify a previously unrecognized regulator of the cell cycle and cyclin D1 expression...
  32. ncbi request reprint Mechanisms for nucleosome mobilization
    Andrew Flaus
    Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Scotland
    Biopolymers 68:563-78. 2003
    ..Either, or both, mechanisms could occur, and careful experimentation focusing on their uniquely distinguishing features will be required to determine their relative contributions to chromatin dynamics...
  33. pmc CENP-A confers a reduction in height on octameric nucleosomes
    Matthew D D Miell
    Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, UK
    Nat Struct Mol Biol 20:763-5. 2013
    ..We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes...
  34. pmc Long distance PELDOR measurements on the histone core particle
    Richard Ward
    School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    J Am Chem Soc 131:1348-9. 2009
    ..Relatively minor differences to the crystal structures have been observed...
  35. pmc Evolutionary insights into genome-wide nucleosome positioning
    Vijender Singh
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    Genome Biol 13:170. 2012
    ..A new study takes an evolutionary approach to investigate to what extent nucleosome positioning is determined by underlying sequence or by trans-acting factors...
  36. ncbi request reprint The TAF(II)250 subunit of TFIID has histone acetyltransferase activity
    C A Mizzen
    Department of Biology, University of Rochester, New York 14627, USA
    Cell 87:1261-70. 1996
    ..Our findings suggest that targeted histone acetylation at specific promoters by TAF(II)250 may be involved in mechanisms by which TFIID gains access to transcriptionally repressed chromatin...
  37. pmc Repression of GCN5 histone acetyltransferase activity via bromodomain-mediated binding and phosphorylation by the Ku-DNA-dependent protein kinase complex
    N A Barlev
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 18:1349-58. 1998
    ..These findings suggest a possible regulatory mechanism of HAT activity...
  38. ncbi request reprint The effect of DnaA protein levels and the rate of initiation at oriC on transcription originating in the ftsQ and ftsA genes: in vivo experiments
    M Masters
    Department of Molecular Biology, University of Edinburgh, Scotland
    Mol Gen Genet 216:475-83. 1989
    ..Strains integratively suppressed with pKN500 behave anomalously; neither fts nor dnaA transcription is significantly increased when DnaA is inactivated in these strains...
  39. ncbi request reprint Histone-like protein H1 (H-NS), DNA supercoiling, and gene expression in bacteria
    C S Hulton
    Imperial Cancer Research Fund, University of Oxford, England
    Cell 63:631-42. 1990
    ..These data imply a regulatory role for one of the major components of bacterial chromatin and provide support for the notion that changes in DNA topology and/or chromatin structure play a role in regulating gene expression...
  40. ncbi request reprint Protein H1: a role for chromatin structure in the regulation of bacterial gene expression and virulence?
    C F Higgins
    ICRF Laboratories, University of Oxford, John Radcliffe Hospital, UK
    Mol Microbiol 4:2007-12. 1990
    ....