R Alan North

Summary

Affiliation: University of Sheffield
Country: UK

Publications

  1. ncbi request reprint Pharmacology of cloned P2X receptors
    R A North
    Institute of Molecular Physiology, University of Sheffield, United Kingdom
    Annu Rev Pharmacol Toxicol 40:563-80. 2000
  2. ncbi request reprint Molecular physiology of P2X receptors
    R Alan North
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield, United Kingdom
    Physiol Rev 82:1013-67. 2002
  3. ncbi request reprint The P2X3 subunit: a molecular target in pain therapeutics
    R Alan North
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK
    Curr Opin Investig Drugs 4:833-40. 2003
  4. pmc P2X3 receptors and peripheral pain mechanisms
    R Alan North
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    J Physiol 554:301-8. 2004
  5. ncbi request reprint N-methyl-D-glucamine and propidium dyes utilize different permeation pathways at rat P2X(7) receptors
    Lin Hua Jiang
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Am J Physiol Cell Physiol 289:C1295-302. 2005
  6. ncbi request reprint Subunit arrangement in P2X receptors
    Lin Hua Jiang
    Institute of Molecular Physiology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Neurosci 23:8903-10. 2003
  7. ncbi request reprint Tissue distribution of P2X4 receptors studied with an ectodomain antibody
    Xuenong Bo
    Institute of Molecular Physiology, University of Sheffield, Sheffield, S10 2TN, UK
    Cell Tissue Res 313:159-65. 2003
  8. ncbi request reprint Pharmacological and biophysical properties of the human P2X5 receptor
    Xuenong Bo
    Institute of Molecular Physiology, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield S10 2TN, UK
    Mol Pharmacol 63:1407-16. 2003
  9. pmc Interaction between cysteines introduced into each transmembrane domain of the rat P2X2 receptor
    Valeria Spelta
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Br J Pharmacol 138:131-6. 2003
  10. ncbi request reprint Reanalysis of P2X7 receptor expression in rodent brain
    Joan A Sim
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    J Neurosci 24:6307-14. 2004

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Pharmacology of cloned P2X receptors
    R A North
    Institute of Molecular Physiology, University of Sheffield, United Kingdom
    Annu Rev Pharmacol Toxicol 40:563-80. 2000
    ..Other agonists, antagonists, and ions that can be used to differentiate among the receptors are discussed...
  2. ncbi request reprint Molecular physiology of P2X receptors
    R Alan North
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield, United Kingdom
    Physiol Rev 82:1013-67. 2002
    ..In the last case, P2X7 receptor activation stimulates cytokine release by engaging intracellular signaling pathways...
  3. ncbi request reprint The P2X3 subunit: a molecular target in pain therapeutics
    R Alan North
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK
    Curr Opin Investig Drugs 4:833-40. 2003
    ..This has been substantiated by the development by Abbott of a small molecule that selectively blocks P2X3 subunit-containing P2X receptors, and which is effective in a range of animal models of neuropathic and chronic inflammatory pain...
  4. pmc P2X3 receptors and peripheral pain mechanisms
    R Alan North
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    J Physiol 554:301-8. 2004
    ..The availability of such tools and experimental approaches promises to accelerate our understanding of the other physiological roles for P2X receptors on primary afferent neurones...
  5. ncbi request reprint N-methyl-D-glucamine and propidium dyes utilize different permeation pathways at rat P2X(7) receptors
    Lin Hua Jiang
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Am J Physiol Cell Physiol 289:C1295-302. 2005
    ..Together, the clear differential effects of extracellular sodium ions or of mutation of the receptor strongly suggest that N-methyl-d-glucamine and YO-PRO-1 do not enter the cell by the same permeation pathway...
  6. ncbi request reprint Subunit arrangement in P2X receptors
    Lin Hua Jiang
    Institute of Molecular Physiology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Neurosci 23:8903-10. 2003
    ..The results suggest a "head-to-tail" subunit arrangement in the quaternary structure of P2X receptors and show that a trimeric P2X2/3 receptor would have the composition P2X2(P2X3)2...
  7. ncbi request reprint Tissue distribution of P2X4 receptors studied with an ectodomain antibody
    Xuenong Bo
    Institute of Molecular Physiology, University of Sheffield, Sheffield, S10 2TN, UK
    Cell Tissue Res 313:159-65. 2003
    ..The findings indicate that the P2X4 receptor subunit has a very extensive distribution among mammalian tissues, and this suggests possible new functional roles...
  8. ncbi request reprint Pharmacological and biophysical properties of the human P2X5 receptor
    Xuenong Bo
    Institute of Molecular Physiology, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield S10 2TN, UK
    Mol Pharmacol 63:1407-16. 2003
    ..Cells expressing human P2X5 receptors also rapidly accumulated the propidium dye YO-PRO-1 in response to ATP...
  9. pmc Interaction between cysteines introduced into each transmembrane domain of the rat P2X2 receptor
    Valeria Spelta
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Br J Pharmacol 138:131-6. 2003
    ..It reversed the rapid current deactivation at F44C/L338C. 5 The results suggest that a methanethiosulfonate binding to L338C prevents binding to F44C; this might indicate proximity of these two residues...
  10. ncbi request reprint Reanalysis of P2X7 receptor expression in rodent brain
    Joan A Sim
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    J Neurosci 24:6307-14. 2004
    ..Either the P2X7 protein in the hippocampus is below the limits of detection by the currently available methods or it is not present...
  11. pmc P2X4, P2Y1 and P2Y2 receptors on rat alveolar macrophages
    Jonathan W Bowler
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Br J Pharmacol 140:567-75. 2003
    ..The P2Y receptors are most likely P2Y1 and P2Y2 and these couple through phospholipase C to an increase in intracellular calcium and the opening of SK type potassium channels...
  12. pmc ATP analogues with modified phosphate chains and their selectivity for rat P2X2 and P2X2/3 receptors
    Valeria Spelta
    Institute of Molecular Physiology, University of Sheffield, Sheffield S10 2TN
    Br J Pharmacol 140:1027-34. 2003
    ..that the heteromeric P2X2/3 receptor is much more tolerant of alphabeta and betagamma substitution; and (c). that a P2X2/3-selective antagonist can be obtained by introduction of additional negativity at the betagamma-methylene...
  13. pmc Methyllycaconitine, alpha-bungarotoxin and (+)-tubocurarine block fast ATP-gated currents in rat dorsal root ganglion cells
    Ulyana Lalo
    Institute of Molecular Physiology, University of Sheffield, Western Bank, Sheffield S10 2TN
    Br J Pharmacol 142:1227-32. 2004
    ..This finding suggests that these drugs should be used with care to discriminate between P2X and neuronal acetylcholine receptor types...
  14. ncbi request reprint Epithelial membrane proteins induce membrane blebbing and interact with the P2X7 receptor C terminus
    Heather L Wilson
    Institute of Molecular Physiology, University of Sheffield S10 2TN, United Kingdom
    J Biol Chem 277:34017-23. 2002
    ..These findings suggest that the P2X(7) C-terminal domain associates with EMPs, and this interaction may mediate some aspects of the downstream signaling following P2X(7) receptor activation...
  15. ncbi request reprint Kv1.3 potassium channels in human alveolar macrophages
    Amanda B Mackenzie
    Inst of Molecular Physiology, Alfred Denny Bldg, Western Bank, Univ of Sheffield, Sheffield S10 2TN, United Kingdom
    Am J Physiol Lung Cell Mol Physiol 285:L862-8. 2003
    ..Under these experimental conditions Kv1.3 sets the resting potential of the cells, but it is not required for Fc receptor-mediated phagocytosis...
  16. ncbi request reprint Sustained depolarization and ADP-ribose activate a common ionic current in rat peritoneal macrophages
    Brice Campo
    Institute of Molecular Physiology, University of Sheffield, Sheffield, United Kingdom
    J Immunol 170:1167-73. 2003
    ..The results suggest that prolonged depolarization leads to an increased intracellular level of ADP-ribose, which in turn activates this nonselective conductance(s)...
  17. pmc Kinetics of antagonist actions at rat P2X2/3 heteromeric receptors
    Valeria Spelta
    Institute of Molecular Physiology, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield S10 2TN, UK
    Br J Pharmacol 135:1524-30. 2002
    ..PPADS (K(D) approximately 0.1 microM) associated and dissociated some 100 - 10,000 times more slowly than the other antagonists...
  18. ncbi request reprint Spontaneous autocrine release of protons activates ASIC-mediated currents in HEK293 cells
    Ulyana Lalo
    Faculty of Life Sciences, The University of Manchester, Manchester, UK
    J Cell Physiol 212:473-80. 2007
    ..We concluded that spontaneous currents represent activation of acid-sensitive ion channels (ASICs) by autocrine vesicular release of protons from HEK cells...
  19. ncbi request reprint P2X receptors as cell-surface ATP sensors in health and disease
    Baljit S Khakh
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 442:527-32. 2006
    ..P2X receptors have a molecular architecture distinct from other ion channel protein families, and have several unique functional properties...
  20. ncbi request reprint An intracellular P2X receptor required for osmoregulation in Dictyostelium discoideum
    Samuel J Fountain
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
    Nature 448:200-3. 2007
    ..Cell swelling in these mutant cells was accompanied by a marked inhibition of contractile vacuole emptying. These findings demonstrate a new functional role for P2X receptors on intracellular organelles, in this case in osmoregulation...
  21. ncbi request reprint Thr339-to-serine substitution in rat P2X2 receptor second transmembrane domain causes constitutive opening and indicates a gating role for Lys308
    Lishuang Cao
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PL, United Kingdom
    J Neurosci 27:12916-23. 2007
    ..The correction of this gating defect, in the absence of any agonist, by the second mutation K308A shows that Lys308 is also involved in channel gating. A similar interpretation can account for the results with suramin and TNP-ATP...
  22. pmc Permeation properties of a P2X receptor in the green algae Ostreococcus tauri
    Samuel J Fountain
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, United Kingdom
    J Biol Chem 283:15122-6. 2008
    ..The results indicate that the ability of ATP to gate cation permeation across membranes exists in cells that diverged in evolutionary terms from animals about 1 billion years ago...
  23. ncbi request reprint A C-terminal lysine that controls human P2X4 receptor desensitization
    Samuel J Fountain
    Faculty of Life Sciences, Michael Smith Building, University of Manchester, Manchester, M13 9PT, United Kingdom
    J Biol Chem 281:15044-9. 2006
    ..These residues lie between previously identified motifs involved in membrane trafficking (YXXXK and YXXGL) and implicates the C-terminal also in rearrangements leading to channel closing during the presence of agonist...
  24. ncbi request reprint Identification of a trafficking motif involved in the stabilization and polarization of P2X receptors
    Severine Chaumont
    Departement de Pharmacologie, Laboratoire de Génomique Fonctionnelle, CNRS UPR2580, 141 rue de la Cardonille, 34396 Montpellier, France
    J Biol Chem 279:29628-38. 2004
    ....
  25. pmc Quantal release of ATP in mouse cortex
    Yuriy Pankratov
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK
    J Gen Physiol 129:257-65. 2007
    ..Furthermore, the packets of ATP appear to be released from vesicle in a subset of glutamate-containing terminals...
  26. doi request reprint P2X1 and P2X5 subunits form the functional P2X receptor in mouse cortical astrocytes
    Ulyana Lalo
    Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, United Kingdom
    J Neurosci 28:5473-80. 2008
    ....
  27. ncbi request reprint Altered hippocampal synaptic potentiation in P2X4 knock-out mice
    Joan A Sim
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Neurosci 26:9006-9. 2006
    ..We suggest that calcium entry through subsynaptic P2X4 receptors during high-frequency stimulation contributes to synaptic strengthening...
  28. ncbi request reprint Role of ectodomain lysines in the subunits of the heteromeric P2X2/3 receptor
    William J Wilkinson
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
    Mol Pharmacol 70:1159-63. 2006
    ..The failure to rescue function in the P2X2 subunit with both lysines mutated by wild-type P2X3 suggests that these residues from two different subunits interact in agonist binding or channel opening...
  29. ncbi request reprint Purinergic transmission in the central nervous system
    R Alan North
    Faculty of Life Sciences, The University of Manchester, 1 124 Stopford Building, Oxford Road, Manchester, M13 9PT, UK
    Pflugers Arch 452:479-85. 2006
    ..In addition, ATP can be released from astroglial cells, thereby acting as a mediator of glial-glial and glial-neuronal signaling...
  30. ncbi request reprint Vesicular release of ATP at central synapses
    Yuri Pankratov
    Faculty of Life Sciences, The University of Manchester, 1 124 Stopford Building, Oxford Road, Manchester, M13 9PT, UK
    Pflugers Arch 452:589-97. 2006
    ....
  31. ncbi request reprint NMDA receptors mediate neuron-to-glia signaling in mouse cortical astrocytes
    Ulyana Lalo
    Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, United Kingdom
    J Neurosci 26:2673-83. 2006
    ..We conclude that cortical astrocytes express functional NMDA receptors that are devoid of Mg2+ block, and these receptors are involved in neuronal-glial signal transmission...
  32. pmc Molecular shape, architecture, and size of P2X4 receptors determined using fluorescence resonance energy transfer and electron microscopy
    Mark T Young
    Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7DN, United Kingdom
    J Biol Chem 283:26241-51. 2008
    ..1 nm, which closely matches FRET data. Thus, our data provide the first views of the architecture, shape, and size of single P2X receptors, furthering our understanding of this important family of ligand-gated ion channels...